@article{6161, abstract = {The tra-1 gene is a terminal regulator of somatic sex in Caenorhabditis elegans: high tra-1 activity elicits female development, low tra-1 activity elicits male development. To investigate the function and evolution of tra- 1, we examined the tra-1 gene from the closely related nematode C. briggsae. Ce-tra-1 and Cb-tra-1 are unusually divergent. Each gene generates two transcripts, but only one of these is present in both species. This common transcript encodes TRA-1A, which shows only 44% amino acid identity between the species, a figure much lower than that for previously compared genes. A Cb-tra-1 transgene rescues many tissues of tra-1(null) mutants of C. elegans but not the somatic gonad or germ line. This transgene also causes nongonadal feminization of XO animals, indicating incorrect sexual regulation. Alignment of Ce-TRA-1A and Cb-TRA-1A defined several conserved regions likely to be important for tra-1 function. The phenotype differences between Ce-tra- 1(null) mutants rescued by Cb-tra-1 transgenes and wild-type C. elegans indicate significant divergence of regulatory regions. These molecular and functional studies suggest that evolution of sex determination in nematodes is rapid and genetically complex.}, author = {de Bono, Mario and Hodgkin, J.}, issn = {00166731}, journal = {Genetics}, keywords = {amino acid sequence, article, caenorhabditis elegans, evolution, genetic variability, nonhuman, priority journal, sex determination, Amino Acid Sequence, Animals, Animals, Genetically Modified, Base Sequence, Caenorhabditis, Caenorhabditis elegans, Caenorhabditis elegans Proteins, DNA, Helminth, DNA-Binding Proteins, Evolution, Molecular, Female, Helminth Proteins, Membrane Proteins, Molecular Sequence Data, Mutagenesis, RNA, Messenger, Sequence Homology, Amino Acid, Sex Determination (Analysis), Transcription Factors, Transgenes, Turner Syndrome, Animalia, Caenorhabditis, Caenorhabditis briggsae, Caenorhabditis elegans, Nematoda}, number = {2}, pages = {587--595}, publisher = {Genetics Society of America}, title = {{Evolution of sex determination in Caenorhabditis: Unusually high divergence of tra-1 and its functional consequences}}, volume = {144}, year = {1996}, }