@inbook{8450, abstract = {Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in E. coli-based protein expression, as exemplified with the mitochondrial carrier GGC.}, author = {Kurauskas, Vilius and Schanda, Paul and Sounier, Remy}, booktitle = {Membrane protein structure and function characterization}, isbn = {9781493971497}, issn = {1064-3745}, pages = {109--123}, publisher = {Springer Nature}, title = {{Methyl-specific isotope labeling strategies for NMR studies of membrane proteins}}, doi = {10.1007/978-1-4939-7151-0_6}, volume = {1635}, year = {2017}, }