--- _id: '8966' abstract: - lang: eng text: During development, a single cell is transformed into a highly complex organism through progressive cell division, specification and rearrangement. An important prerequisite for the emergence of patterns within the developing organism is to establish asymmetries at various scales, ranging from individual cells to the entire embryo, eventually giving rise to the different body structures. This becomes especially apparent during gastrulation, when the earliest major lineage restriction events lead to the formation of the different germ layers. Traditionally, the unfolding of the developmental program from symmetry breaking to germ layer formation has been studied by dissecting the contributions of different signaling pathways and cellular rearrangements in the in vivo context of intact embryos. Recent efforts, using the intrinsic capacity of embryonic stem cells to self-assemble and generate embryo-like structures de novo, have opened new avenues for understanding the many ways by which an embryo can be built and the influence of extrinsic factors therein. Here, we discuss and compare divergent and conserved strategies leading to germ layer formation in embryos as compared to in vitro systems, their upstream molecular cascades and the role of extrinsic factors in this process. acknowledgement: We thank Nicoletta Petridou, Diana Pinheiro, Cornelia Schwayer and Stefania Tavano for feedback on the manuscript. Research in the Heisenberg lab is supported by an ERC Advanced Grant (MECSPEC 742573) to C.-P.H. A.S. is a recipient of a DOC Fellowship of the Austrian Academy of Science. article_processing_charge: Yes (via OA deal) article_type: original author: - first_name: Alexandra full_name: Schauer, Alexandra id: 30A536BA-F248-11E8-B48F-1D18A9856A87 last_name: Schauer orcid: 0000-0001-7659-9142 - first_name: Carl-Philipp J full_name: Heisenberg, Carl-Philipp J id: 39427864-F248-11E8-B48F-1D18A9856A87 last_name: Heisenberg orcid: 0000-0002-0912-4566 citation: ama: Schauer A, Heisenberg C-PJ. Reassembling gastrulation. Developmental Biology. 2021;474:71-81. doi:10.1016/j.ydbio.2020.12.014 apa: Schauer, A., & Heisenberg, C.-P. J. (2021). Reassembling gastrulation. Developmental Biology. Elsevier. https://doi.org/10.1016/j.ydbio.2020.12.014 chicago: Schauer, Alexandra, and Carl-Philipp J Heisenberg. “Reassembling Gastrulation.” Developmental Biology. Elsevier, 2021. https://doi.org/10.1016/j.ydbio.2020.12.014. ieee: A. Schauer and C.-P. J. Heisenberg, “Reassembling gastrulation,” Developmental Biology, vol. 474. Elsevier, pp. 71–81, 2021. ista: Schauer A, Heisenberg C-PJ. 2021. Reassembling gastrulation. Developmental Biology. 474, 71–81. mla: Schauer, Alexandra, and Carl-Philipp J. Heisenberg. “Reassembling Gastrulation.” Developmental Biology, vol. 474, Elsevier, 2021, pp. 71–81, doi:10.1016/j.ydbio.2020.12.014. short: A. Schauer, C.-P.J. Heisenberg, Developmental Biology 474 (2021) 71–81. date_created: 2020-12-22T09:53:34Z date_published: 2021-06-01T00:00:00Z date_updated: 2023-08-07T13:30:01Z day: '01' ddc: - '570' department: - _id: CaHe doi: 10.1016/j.ydbio.2020.12.014 ec_funded: 1 external_id: isi: - '000639461800008' file: - access_level: open_access checksum: fa2a5731fd16ab171b029f32f031c440 content_type: application/pdf creator: kschuh date_created: 2021-08-11T10:28:06Z date_updated: 2021-08-11T10:28:06Z file_id: '9880' file_name: 2021_DevBiology_Schauer.pdf file_size: 1440321 relation: main_file success: 1 file_date_updated: 2021-08-11T10:28:06Z has_accepted_license: '1' intvolume: ' 474' isi: 1 keyword: - Developmental Biology - Cell Biology - Molecular Biology language: - iso: eng license: https://creativecommons.org/licenses/by-nc-nd/4.0/ month: '06' oa: 1 oa_version: Published Version page: 71-81 project: - _id: 260F1432-B435-11E9-9278-68D0E5697425 call_identifier: H2020 grant_number: '742573' name: Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation - _id: 26B1E39C-B435-11E9-9278-68D0E5697425 grant_number: '25239' name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues' publication: Developmental Biology publication_identifier: issn: - 0012-1606 publication_status: published publisher: Elsevier quality_controlled: '1' related_material: record: - id: '12891' relation: dissertation_contains status: public scopus_import: '1' status: public title: Reassembling gastrulation tmp: image: /images/cc_by_nc_nd.png legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) short: CC BY-NC-ND (4.0) type: journal_article user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8 volume: 474 year: '2021' ... --- _id: '7545' abstract: - lang: eng text: Neuronal activity often leads to alterations in gene expression and cellular architecture. The nematode Caenorhabditis elegans, owing to its compact translucent nervous system, is a powerful system in which to study conserved aspects of the development and plasticity of neuronal morphology. Here we focus on one pair of sensory neurons, termed URX, which the worm uses to sense and avoid high levels of environmental oxygen. Previous studies have reported that the URX neuron pair has variable branched endings at its dendritic sensory tip. By controlling oxygen levels and analyzing mutants, we found that these microtubule-rich branched endings grow over time as a consequence of neuronal activity in adulthood. We also find that the growth of these branches correlates with an increase in cellular sensitivity to particular ranges of oxygen that is observable in the behavior of older worms. Given the strengths of C. elegans as a model organism, URX may serve as a potent system for uncovering genes and mechanisms involved in activity-dependent morphological changes in neurons and possible adaptive changes in the aging nervous system. article_processing_charge: No article_type: original author: - first_name: Jesse A. full_name: Cohn, Jesse A. last_name: Cohn - first_name: Elizabeth R. full_name: Cebul, Elizabeth R. last_name: Cebul - first_name: Giulio full_name: Valperga, Giulio last_name: Valperga - first_name: Lotti full_name: Brose, Lotti last_name: Brose - first_name: Mario full_name: de Bono, Mario id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87 last_name: de Bono orcid: 0000-0001-8347-0443 - first_name: Maxwell G. full_name: Heiman, Maxwell G. last_name: Heiman - first_name: Jonathan T. full_name: Pierce, Jonathan T. last_name: Pierce citation: ama: Cohn JA, Cebul ER, Valperga G, et al. Long-term activity drives dendritic branch elaboration of a C. elegans sensory neuron. Developmental Biology. 2020;461(1):66-74. doi:10.1016/j.ydbio.2020.01.005 apa: Cohn, J. A., Cebul, E. R., Valperga, G., Brose, L., de Bono, M., Heiman, M. G., & Pierce, J. T. (2020). Long-term activity drives dendritic branch elaboration of a C. elegans sensory neuron. Developmental Biology. Elsevier. https://doi.org/10.1016/j.ydbio.2020.01.005 chicago: Cohn, Jesse A., Elizabeth R. Cebul, Giulio Valperga, Lotti Brose, Mario de Bono, Maxwell G. Heiman, and Jonathan T. Pierce. “Long-Term Activity Drives Dendritic Branch Elaboration of a C. Elegans Sensory Neuron.” Developmental Biology. Elsevier, 2020. https://doi.org/10.1016/j.ydbio.2020.01.005. ieee: J. A. Cohn et al., “Long-term activity drives dendritic branch elaboration of a C. elegans sensory neuron,” Developmental Biology, vol. 461, no. 1. Elsevier, pp. 66–74, 2020. ista: Cohn JA, Cebul ER, Valperga G, Brose L, de Bono M, Heiman MG, Pierce JT. 2020. Long-term activity drives dendritic branch elaboration of a C. elegans sensory neuron. Developmental Biology. 461(1), 66–74. mla: Cohn, Jesse A., et al. “Long-Term Activity Drives Dendritic Branch Elaboration of a C. Elegans Sensory Neuron.” Developmental Biology, vol. 461, no. 1, Elsevier, 2020, pp. 66–74, doi:10.1016/j.ydbio.2020.01.005. short: J.A. Cohn, E.R. Cebul, G. Valperga, L. Brose, M. de Bono, M.G. Heiman, J.T. Pierce, Developmental Biology 461 (2020) 66–74. date_created: 2020-02-28T10:38:32Z date_published: 2020-05-01T00:00:00Z date_updated: 2021-01-12T08:14:06Z day: '01' doi: 10.1016/j.ydbio.2020.01.005 extern: '1' intvolume: ' 461' issue: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1101/685339 month: '05' oa: 1 oa_version: Preprint page: 66-74 publication: Developmental Biology publication_identifier: issn: - 0012-1606 publication_status: published publisher: Elsevier quality_controlled: '1' status: public title: Long-term activity drives dendritic branch elaboration of a C. elegans sensory neuron type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 461 year: '2020' ...