---
_id: '1209'
abstract:
- lang: eng
text: 'NADH-ubiquinone oxidoreductase (complex I) is the largest (∼1 MDa) and the
least characterized complex of the mitochondrial electron transport chain. Because
of the ease of sample availability, previous work has focused almost exclusively
on bovine complex I. However, only medium resolution structural analyses of this
complex have been reported. Working with other mammalian complex I homologues
is a potential approach for overcoming these limitations. Due to the inherent
difficulty of expressing large membrane protein complexes, screening of complex
I homologues is limited to large mammals reared for human consumption. The high
sequence identity among these available sources may preclude the benefits of screening.
Here, we report the characterization of complex I purified from Ovis aries (ovine)
heart mitochondria. All 44 unique subunits of the intact complex were identified
by mass spectrometry. We identified differences in the subunit composition of
subcomplexes of ovine complex I as compared with bovine, suggesting differential
stability of inter-subunit interactions within the complex. Furthermore, the 42-kDa
subunit, which is easily lost from the bovine enzyme, remains tightly bound to
ovine complex I. Additionally, we developed a novel purification protocol for
highly active and stable mitochondrial complex I using the branched-chain detergent
lauryl maltose neopentyl glycol. Our data demonstrate that, although closely related,
significant differences exist between the biochemical properties of complex I
prepared from ovine and bovine mitochondria and that ovine complex I represents
a suitable alternative target for further structural studies. '
acknowledgement: "J.A.S supported in part by a Medical Research D.G.Council UK Ph.D.
fellowship.\r\nThis work was supported in part by European Union's 2020 Research
and Innovation Program under Grant 701309. \r\n"
author:
- first_name: James A
full_name: Letts, James A
id: 322DA418-F248-11E8-B48F-1D18A9856A87
last_name: Letts
orcid: 0000-0002-9864-3586
- first_name: Gianluca
full_name: Degliesposti, Gianluca
last_name: Degliesposti
- first_name: Karol
full_name: Fiedorczuk, Karol
id: 5BFF67CE-02D1-11E9-B11A-A5A4D7DFFFD0
last_name: Fiedorczuk
- first_name: Mark
full_name: Skehel, Mark
last_name: Skehel
- first_name: Leonid A
full_name: Sazanov, Leonid A
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
citation:
ama: Letts JA, Degliesposti G, Fiedorczuk K, Skehel M, Sazanov LA. Purification
of ovine respiratory complex i results in a highly active and stable preparation.
Journal of Biological Chemistry. 2016;291(47):24657-24675. doi:10.1074/jbc.M116.735142
apa: Letts, J. A., Degliesposti, G., Fiedorczuk, K., Skehel, M., & Sazanov,
L. A. (2016). Purification of ovine respiratory complex i results in a highly
active and stable preparation. Journal of Biological Chemistry. American
Society for Biochemistry and Molecular Biology. https://doi.org/10.1074/jbc.M116.735142
chicago: Letts, James A, Gianluca Degliesposti, Karol Fiedorczuk, Mark Skehel, and
Leonid A Sazanov. “Purification of Ovine Respiratory Complex i Results in a Highly
Active and Stable Preparation.” Journal of Biological Chemistry. American
Society for Biochemistry and Molecular Biology, 2016. https://doi.org/10.1074/jbc.M116.735142.
ieee: J. A. Letts, G. Degliesposti, K. Fiedorczuk, M. Skehel, and L. A. Sazanov,
“Purification of ovine respiratory complex i results in a highly active and stable
preparation,” Journal of Biological Chemistry, vol. 291, no. 47. American
Society for Biochemistry and Molecular Biology, pp. 24657–24675, 2016.
ista: Letts JA, Degliesposti G, Fiedorczuk K, Skehel M, Sazanov LA. 2016. Purification
of ovine respiratory complex i results in a highly active and stable preparation.
Journal of Biological Chemistry. 291(47), 24657–24675.
mla: Letts, James A., et al. “Purification of Ovine Respiratory Complex i Results
in a Highly Active and Stable Preparation.” Journal of Biological Chemistry,
vol. 291, no. 47, American Society for Biochemistry and Molecular Biology, 2016,
pp. 24657–75, doi:10.1074/jbc.M116.735142.
short: J.A. Letts, G. Degliesposti, K. Fiedorczuk, M. Skehel, L.A. Sazanov, Journal
of Biological Chemistry 291 (2016) 24657–24675.
date_created: 2018-12-11T11:50:44Z
date_published: 2016-11-18T00:00:00Z
date_updated: 2021-01-12T06:49:06Z
day: '18'
department:
- _id: LeSa
doi: 10.1074/jbc.M116.735142
ec_funded: 1
intvolume: ' 291'
issue: '47'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114416/
month: '11'
oa: 1
oa_version: Submitted Version
page: 24657 - 24675
project:
- _id: 2593EBD6-B435-11E9-9278-68D0E5697425
name: Atomic-Resolution Structures of Mitochondrial Respiratory Chain Supercomplexes
(FEBS)
- _id: 2590DB08-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '701309'
name: Atomic-Resolution Structures of Mitochondrial Respiratory Chain Supercomplexes
(H2020)
publication: Journal of Biological Chemistry
publication_status: published
publisher: American Society for Biochemistry and Molecular Biology
publist_id: '6139'
quality_controlled: '1'
scopus_import: 1
status: public
title: Purification of ovine respiratory complex i results in a highly active and
stable preparation
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 291
year: '2016'
...
---
_id: '1210'
abstract:
- lang: eng
text: Mechanisms for cell protection are essential for survival of multicellular
organisms. In plants, the apical hook, which is transiently formed in darkness
when the germinating seedling penetrates towards the soil surface, plays such
protective role and shields the vitally important shoot apical meristem and cotyledons
from damage. The apical hook is formed by bending of the upper hypocotyl soon
after germination, and it is maintained in a closed stage while the hypocotyl
continues to penetrate through the soil and rapidly opens when exposed to light
in proximity of the soil surface. To uncover the complex molecular network orchestrating
this spatiotemporally tightly coordinated process, monitoring of the apical hook
development in real time is indispensable. Here we describe an imaging platform
that enables high-resolution kinetic analysis of this dynamic developmental process.
© Springer Science+Business Media New York 2017.
acknowledgement: "We thank Herman \r\nHöfte \r\n, Todor Asenov, Robert Hauschield,
and \r\nMarcal Gallemi for help with the establishment of the real-time
\ \r\nimaging platform and technical support. This work was supported \r\nby the
Czech Science Foundation (GA13-39982S) to Eva Benková. \r\nDominique Van Der
\ Straeten acknowledges the Research \r\nFoundation Flanders for fi\r\n
\ nancial support (G.0656.13N). Dajo \r\nSmet holds a PhD fellowship of the
Research Foundation Flanders. "
alternative_title:
- Methods in Molecular Biology
author:
- first_name: Qiang
full_name: Zhu, Qiang
id: 40A4B9E6-F248-11E8-B48F-1D18A9856A87
last_name: Zhu
- first_name: Petra
full_name: Žádníková, Petra
last_name: Žádníková
- first_name: Dajo
full_name: Smet, Dajo
last_name: Smet
- first_name: Dominique
full_name: Van Der Straeten, Dominique
last_name: Van Der Straeten
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: 'Zhu Q, Žádníková P, Smet D, Van Der Straeten D, Benková E. Real time analysis
of the apical hook development. In: Plant Hormones. Vol 1497. Humana Press;
2016:1-8. doi:10.1007/978-1-4939-6469-7_1'
apa: Zhu, Q., Žádníková, P., Smet, D., Van Der Straeten, D., & Benková, E. (2016).
Real time analysis of the apical hook development. In Plant Hormones (Vol.
1497, pp. 1–8). Humana Press. https://doi.org/10.1007/978-1-4939-6469-7_1
chicago: Zhu, Qiang, Petra Žádníková, Dajo Smet, Dominique Van Der Straeten, and
Eva Benková. “Real Time Analysis of the Apical Hook Development.” In Plant
Hormones, 1497:1–8. Humana Press, 2016. https://doi.org/10.1007/978-1-4939-6469-7_1.
ieee: Q. Zhu, P. Žádníková, D. Smet, D. Van Der Straeten, and E. Benková, “Real
time analysis of the apical hook development,” in Plant Hormones, vol.
1497, Humana Press, 2016, pp. 1–8.
ista: 'Zhu Q, Žádníková P, Smet D, Van Der Straeten D, Benková E. 2016.Real time
analysis of the apical hook development. In: Plant Hormones. Methods in Molecular
Biology, vol. 1497, 1–8.'
mla: Zhu, Qiang, et al. “Real Time Analysis of the Apical Hook Development.” Plant
Hormones, vol. 1497, Humana Press, 2016, pp. 1–8, doi:10.1007/978-1-4939-6469-7_1.
short: Q. Zhu, P. Žádníková, D. Smet, D. Van Der Straeten, E. Benková, in:, Plant
Hormones, Humana Press, 2016, pp. 1–8.
date_created: 2018-12-11T11:50:44Z
date_published: 2016-11-19T00:00:00Z
date_updated: 2021-01-12T06:49:07Z
day: '19'
department:
- _id: EvBe
doi: 10.1007/978-1-4939-6469-7_1
intvolume: ' 1497'
language:
- iso: eng
month: '11'
oa_version: None
page: 1 - 8
publication: Plant Hormones
publication_status: published
publisher: Humana Press
publist_id: '6135'
quality_controlled: '1'
scopus_import: 1
status: public
title: Real time analysis of the apical hook development
type: book_chapter
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 1497
year: '2016'
...
---
_id: '1212'
abstract:
- lang: eng
text: 'Plants adjust their growth according to gravity. Gravitropism involves gravity
perception, signal transduction, and asymmetric growth response, with organ bending
as a consequence [1]. Asymmetric growth results from the asymmetric distribution
of the plant-specific signaling molecule auxin [2] that is generated by lateral
transport, mediated in the hypocotyl predominantly by the auxin transporter PIN-FORMED3
(PIN3) [3–5]. Gravity stimulation polarizes PIN3 to the bottom sides of endodermal
cells, correlating with increased auxin accumulation in adjacent tissues at the
lower side of the stimulated organ, where auxin induces cell elongation and, hence,
organ bending. A curvature response allows the hypocotyl to resume straight growth
at a defined angle [6], implying that at some point auxin symmetry is restored
to prevent overbending. Here, we present initial insights into cellular and molecular
mechanisms that lead to the termination of the tropic response. We identified
an auxin feedback on PIN3 polarization as underlying mechanism that restores symmetry
of the PIN3-dependent auxin flow. Thus, two mechanistically distinct PIN3 polarization
events redirect auxin fluxes at different time points of the gravity response:
first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower
hypocotyl side, where auxin gradually accumulates and promotes growth, and later
PIN3 polarization to the opposite cell side, depleting this auxin maximum to end
the bending. Accordingly, genetic or pharmacological interference with the late
PIN3 polarization prevents termination of the response and leads to hypocotyl
overbending. This observation reveals a role of auxin feedback on PIN polarity
in the termination of the tropic response. © 2016 Elsevier Ltd'
acknowledgement: "We thank Dr. Jie Li (Key Laboratory of Plant Molecular Physiology,
Chinese Academy of Science, China) for the pPIN3::PIN3-GFP/DII::VENUS line and Martine
De Cock for help in preparing the manuscript. This work was supported by the European
Research Council (project ERC-2011-StG-20101109-PSDP), by the Czech Science Foundation
GAČR (GA13-40637S) to J.F., and by the Ministry of Education, Youth and Sports of
the Czech Republic under the project CEITEC 2020 (LQ1601) to H.S.R. H.R. is indebted
to the Agency for Innovation by Science and Technology (IWT) for a predoctoral fellowship.\r\n"
author:
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
- first_name: Mohamad
full_name: Abbas, Mohamad
id: 47E8FC1C-F248-11E8-B48F-1D18A9856A87
last_name: Abbas
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Siyuan
full_name: Song, Siyuan
last_name: Song
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. Termination of shoot
gravitropic responses by auxin feedback on PIN3 polarity. Current Biology.
2016;26(22):3026-3032. doi:10.1016/j.cub.2016.08.067
apa: Rakusová, H., Abbas, M., Han, H., Song, S., Robert, H., & Friml, J. (2016).
Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity.
Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2016.08.067
chicago: Rakusová, Hana, Mohamad Abbas, Huibin Han, Siyuan Song, Hélène Robert,
and Jiří Friml. “Termination of Shoot Gravitropic Responses by Auxin Feedback
on PIN3 Polarity.” Current Biology. Cell Press, 2016. https://doi.org/10.1016/j.cub.2016.08.067.
ieee: H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, and J. Friml, “Termination
of shoot gravitropic responses by auxin feedback on PIN3 polarity,” Current
Biology, vol. 26, no. 22. Cell Press, pp. 3026–3032, 2016.
ista: Rakusová H, Abbas M, Han H, Song S, Robert H, Friml J. 2016. Termination of
shoot gravitropic responses by auxin feedback on PIN3 polarity. Current Biology.
26(22), 3026–3032.
mla: Rakusová, Hana, et al. “Termination of Shoot Gravitropic Responses by Auxin
Feedback on PIN3 Polarity.” Current Biology, vol. 26, no. 22, Cell Press,
2016, pp. 3026–32, doi:10.1016/j.cub.2016.08.067.
short: H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, J. Friml, Current Biology
26 (2016) 3026–3032.
date_created: 2018-12-11T11:50:44Z
date_published: 2016-11-21T00:00:00Z
date_updated: 2021-01-12T06:49:08Z
day: '21'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1016/j.cub.2016.08.067
ec_funded: 1
file:
- access_level: open_access
checksum: 79ed2498185a027cf51a8f88100379e6
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:33Z
date_updated: 2020-07-14T12:44:39Z
file_id: '4757'
file_name: IST-2018-1008-v1+1_Rakusova_CurrBiol_2016_proof.pdf
file_size: 5391923
relation: main_file
file_date_updated: 2020-07-14T12:44:39Z
has_accepted_license: '1'
intvolume: ' 26'
issue: '22'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Submitted Version
page: 3026 - 3032
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '6138'
pubrep_id: '1008'
quality_controlled: '1'
scopus_import: 1
status: public
title: Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 26
year: '2016'
...
---
_id: '1214'
abstract:
- lang: eng
text: 'With the accelerated development of robot technologies, optimal control becomes
one of the central themes of research. In traditional approaches, the controller,
by its internal functionality, finds appropriate actions on the basis of the history
of sensor values, guided by the goals, intentions, objectives, learning schemes,
and so forth. While very successful with classical robots, these methods run into
severe difficulties when applied to soft robots, a new field of robotics with
large interest for human-robot interaction. We claim that a novel controller paradigm
opens new perspective for this field. This paper applies a recently developed
neuro controller with differential extrinsic synaptic plasticity to a muscle-tendon
driven arm-shoulder system from the Myorobotics toolkit. In the experiments, we
observe a vast variety of self-organized behavior patterns: when left alone, the
arm realizes pseudo-random sequences of different poses. By applying physical
forces, the system can be entrained into definite motion patterns like wiping
a table. Most interestingly, after attaching an object, the controller gets in
a functional resonance with the object''s internal dynamics, starting to shake
spontaneously bottles half-filled with water or sensitively driving an attached
pendulum into a circular mode. When attached to the crank of a wheel the neural
system independently develops to rotate it. In this way, the robot discovers affordances
of objects its body is interacting with.'
acknowledgement: RD thanks for the hospitality at the Max-Planck-Institute and for
helpful discussions with Nihat Ay and Keyan Zahedi.
article_number: '7759138'
author:
- first_name: Georg S
full_name: Martius, Georg S
id: 3A276B68-F248-11E8-B48F-1D18A9856A87
last_name: Martius
- first_name: Raphael
full_name: Hostettler, Raphael
last_name: Hostettler
- first_name: Alois
full_name: Knoll, Alois
last_name: Knoll
- first_name: Ralf
full_name: Der, Ralf
last_name: Der
citation:
ama: 'Martius GS, Hostettler R, Knoll A, Der R. Compliant control for soft robots:
Emergent behavior of a tendon driven anthropomorphic arm. In: Vol 2016-November.
IEEE; 2016. doi:10.1109/IROS.2016.7759138'
apa: 'Martius, G. S., Hostettler, R., Knoll, A., & Der, R. (2016). Compliant
control for soft robots: Emergent behavior of a tendon driven anthropomorphic
arm (Vol. 2016–November). Presented at the IEEE RSJ International Conference on
Intelligent Robots and Systems IROS , Daejeon, Korea: IEEE. https://doi.org/10.1109/IROS.2016.7759138'
chicago: 'Martius, Georg S, Raphael Hostettler, Alois Knoll, and Ralf Der. “Compliant
Control for Soft Robots: Emergent Behavior of a Tendon Driven Anthropomorphic
Arm,” Vol. 2016–November. IEEE, 2016. https://doi.org/10.1109/IROS.2016.7759138.'
ieee: 'G. S. Martius, R. Hostettler, A. Knoll, and R. Der, “Compliant control for
soft robots: Emergent behavior of a tendon driven anthropomorphic arm,” presented
at the IEEE RSJ International Conference on Intelligent Robots and Systems IROS
, Daejeon, Korea, 2016, vol. 2016–November.'
ista: 'Martius GS, Hostettler R, Knoll A, Der R. 2016. Compliant control for soft
robots: Emergent behavior of a tendon driven anthropomorphic arm. IEEE RSJ International
Conference on Intelligent Robots and Systems IROS vol. 2016–November, 7759138.'
mla: 'Martius, Georg S., et al. Compliant Control for Soft Robots: Emergent Behavior
of a Tendon Driven Anthropomorphic Arm. Vol. 2016–November, 7759138, IEEE,
2016, doi:10.1109/IROS.2016.7759138.'
short: G.S. Martius, R. Hostettler, A. Knoll, R. Der, in:, IEEE, 2016.
conference:
end_date: 2016-09-14
location: Daejeon, Korea
name: 'IEEE RSJ International Conference on Intelligent Robots and Systems IROS '
start_date: 2016-09-09
date_created: 2018-12-11T11:50:45Z
date_published: 2016-11-28T00:00:00Z
date_updated: 2021-01-12T06:49:08Z
day: '28'
department:
- _id: ChLa
- _id: GaTk
doi: 10.1109/IROS.2016.7759138
language:
- iso: eng
month: '11'
oa_version: None
publication_status: published
publisher: IEEE
publist_id: '6121'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Compliant control for soft robots: Emergent behavior of a tendon driven anthropomorphic
arm'
type: conference
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 2016-November
year: '2016'
...
---
_id: '1216'
abstract:
- lang: eng
text: 'A framework fo r extracting features in 2D transient flows, based on the
acceleration field to ensure Galilean invariance is proposed in this paper. The
minima of the acceleration magnitude (a superset of acceleration zeros) are extracted
and discriminated into vortices and saddle points, based on the spectral properties
of the velocity Jacobian. The extraction of topological features is performed
with purely combinatorial algorithms from discrete computational topology. The
feature points are prioritized with persistence, as a physically meaningful importance
measure. These feature points are tracked in time with a robust algorithm for
tracking features. Thus, a space-time hierarchy of the minima is built and vortex
merging events are detected. We apply the acceleration feature extraction strategy
to three two-dimensional shear flows: (1) an incompressible periodic cylinder
wake, (2) an incompressible planar mixing layer and (3) a weakly compressible
planar jet. The vortex-like acceleration feature points are shown to be well aligned
with acceleration zeros, maxima of the vorticity magnitude, minima of the pressure
field and minima of λ2.'
acknowledgement: "The authors acknowledge funding of the German Re-\r\nsearch Foundation
\ (DFG) via the Collaborative Re-\r\nsearch Center (SFB 557) \\Control of
\ Complex Turbu-\r\nlent Shear Flows\" and the Emmy Noether Program.\r\nFurther
\ funding was provided by the Zuse Institute\r\nBerlin (ZIB), the DFG-CNRS
\ research group \\Noise\r\nGeneration in Turbulent Flows\" (2003{2010), the Chaire\r\nd'Excellence
'Closed-loop control of turbulent shear ows\r\nusing reduced-order models' (TUCOROM)
of the French\r\nAgence Nationale de la Recherche (ANR), and the Eu-\r\nropean Social
\ Fund (ESF App. No. 100098251). We\r\nthank the Ambrosys Ltd. Society
\ for Complex Sys-\r\ntems Management and the Bernd R. Noack Cybernet-\r\nics
\ Foundation for additional support. A part of this\r\nwork was performed
using HPC resources from GENCI-[CCRT/CINES/IDRIS] supported by the Grant 2011-\r\n[x2011020912"
author:
- first_name: Jens
full_name: Kasten, Jens
last_name: Kasten
- first_name: Jan
full_name: Reininghaus, Jan
id: 4505473A-F248-11E8-B48F-1D18A9856A87
last_name: Reininghaus
- first_name: Ingrid
full_name: Hotz, Ingrid
last_name: Hotz
- first_name: Hans
full_name: Hege, Hans
last_name: Hege
- first_name: Bernd
full_name: Noack, Bernd
last_name: Noack
- first_name: Guillaume
full_name: Daviller, Guillaume
last_name: Daviller
- first_name: Marek
full_name: Morzyński, Marek
last_name: Morzyński
citation:
ama: Kasten J, Reininghaus J, Hotz I, et al. Acceleration feature points of unsteady
shear flows. Archives of Mechanics. 2016;68(1):55-80.
apa: Kasten, J., Reininghaus, J., Hotz, I., Hege, H., Noack, B., Daviller, G., &
Morzyński, M. (2016). Acceleration feature points of unsteady shear flows. Archives
of Mechanics. Polish Academy of Sciences Publishing House.
chicago: Kasten, Jens, Jan Reininghaus, Ingrid Hotz, Hans Hege, Bernd Noack, Guillaume
Daviller, and Marek Morzyński. “Acceleration Feature Points of Unsteady Shear
Flows.” Archives of Mechanics. Polish Academy of Sciences Publishing House,
2016.
ieee: J. Kasten et al., “Acceleration feature points of unsteady shear flows,”
Archives of Mechanics, vol. 68, no. 1. Polish Academy of Sciences Publishing
House, pp. 55–80, 2016.
ista: Kasten J, Reininghaus J, Hotz I, Hege H, Noack B, Daviller G, Morzyński M.
2016. Acceleration feature points of unsteady shear flows. Archives of Mechanics.
68(1), 55–80.
mla: Kasten, Jens, et al. “Acceleration Feature Points of Unsteady Shear Flows.”
Archives of Mechanics, vol. 68, no. 1, Polish Academy of Sciences Publishing
House, 2016, pp. 55–80.
short: J. Kasten, J. Reininghaus, I. Hotz, H. Hege, B. Noack, G. Daviller, M. Morzyński,
Archives of Mechanics 68 (2016) 55–80.
date_created: 2018-12-11T11:50:46Z
date_published: 2016-01-01T00:00:00Z
date_updated: 2021-01-12T06:49:09Z
day: '01'
department:
- _id: HeEd
intvolume: ' 68'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://am.ippt.pan.pl/am/article/viewFile/v68p55/pdf
month: '01'
oa: 1
oa_version: Published Version
page: 55 - 80
publication: Archives of Mechanics
publication_status: published
publisher: Polish Academy of Sciences Publishing House
publist_id: '6118'
quality_controlled: '1'
scopus_import: 1
status: public
title: Acceleration feature points of unsteady shear flows
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 68
year: '2016'
...
---
_id: '1217'
abstract:
- lang: eng
text: Understanding the regulation of T-cell responses during inflammation and auto-immunity
is fundamental for designing efficient therapeutic strategies against immune diseases.
In this regard, prostaglandin E 2 (PGE 2) is mostly considered a myeloid-derived
immunosuppressive molecule. We describe for the first time that T cells secrete
PGE 2 during T-cell receptor stimulation. In addition, we show that autocrine
PGE 2 signaling through EP receptors is essential for optimal CD4 + T-cell activation
in vitro and in vivo, and for T helper 1 (Th1) and regulatory T cell differentiation.
PGE 2 was found to provide additive co-stimulatory signaling through AKT activation.
Intravital multiphoton microscopy showed that triggering EP receptors in T cells
is also essential for the stability of T cell-dendritic cell (DC) interactions
and Th-cell accumulation in draining lymph nodes (LNs) during inflammation. We
further demonstrated that blocking EP receptors in T cells during the initial
phase of collagen-induced arthritis in mice resulted in a reduction of clinical
arthritis. This could be attributable to defective T-cell activation, accompanied
by a decline in activated and interferon-γ-producing CD4 + Th1 cells in draining
LNs. In conclusion, we prove that T lymphocytes secret picomolar concentrations
of PGE 2, which in turn provide additive co-stimulatory signaling, enabling T
cells to attain a favorable activation threshold. PGE 2 signaling in T cells is
also required for maintaining long and stable interactions with DCs within LNs.
Blockade of EP receptors in vivo impairs T-cell activation and development of
T cell-mediated inflammatory responses. This may have implications in various
pathophysiological settings.
acknowledgement: This manuscript has been supported by grants SAF2007-61716 and S-SAL-0159/2006
awarded by the Spanish Ministry of Science and Education and the Community of Madrid
to Dr M Fresno.
author:
- first_name: Vinatha
full_name: Sreeramkumar, Vinatha
last_name: Sreeramkumar
- first_name: Miroslav
full_name: Hons, Miroslav
id: 4167FE56-F248-11E8-B48F-1D18A9856A87
last_name: Hons
orcid: 0000-0002-6625-3348
- first_name: Carmen
full_name: Punzón, Carmen
last_name: Punzón
- first_name: Jens
full_name: Stein, Jens
last_name: Stein
- first_name: David
full_name: Sancho, David
last_name: Sancho
- first_name: Manuel
full_name: Fresno Forcelledo, Manuel
last_name: Fresno Forcelledo
- first_name: Natalia
full_name: Cuesta, Natalia
last_name: Cuesta
citation:
ama: Sreeramkumar V, Hons M, Punzón C, et al. Efficient T-cell priming and activation
requires signaling through prostaglandin E2 (EP) receptors. Immunology and
Cell Biology. 2016;94(1):39-51. doi:10.1038/icb.2015.62
apa: Sreeramkumar, V., Hons, M., Punzón, C., Stein, J., Sancho, D., Fresno Forcelledo,
M., & Cuesta, N. (2016). Efficient T-cell priming and activation requires
signaling through prostaglandin E2 (EP) receptors. Immunology and Cell Biology.
Nature Publishing Group. https://doi.org/10.1038/icb.2015.62
chicago: Sreeramkumar, Vinatha, Miroslav Hons, Carmen Punzón, Jens Stein, David
Sancho, Manuel Fresno Forcelledo, and Natalia Cuesta. “Efficient T-Cell Priming
and Activation Requires Signaling through Prostaglandin E2 (EP) Receptors.” Immunology
and Cell Biology. Nature Publishing Group, 2016. https://doi.org/10.1038/icb.2015.62.
ieee: V. Sreeramkumar et al., “Efficient T-cell priming and activation requires
signaling through prostaglandin E2 (EP) receptors,” Immunology and Cell Biology,
vol. 94, no. 1. Nature Publishing Group, pp. 39–51, 2016.
ista: Sreeramkumar V, Hons M, Punzón C, Stein J, Sancho D, Fresno Forcelledo M,
Cuesta N. 2016. Efficient T-cell priming and activation requires signaling through
prostaglandin E2 (EP) receptors. Immunology and Cell Biology. 94(1), 39–51.
mla: Sreeramkumar, Vinatha, et al. “Efficient T-Cell Priming and Activation Requires
Signaling through Prostaglandin E2 (EP) Receptors.” Immunology and Cell Biology,
vol. 94, no. 1, Nature Publishing Group, 2016, pp. 39–51, doi:10.1038/icb.2015.62.
short: V. Sreeramkumar, M. Hons, C. Punzón, J. Stein, D. Sancho, M. Fresno Forcelledo,
N. Cuesta, Immunology and Cell Biology 94 (2016) 39–51.
date_created: 2018-12-11T11:50:46Z
date_published: 2016-01-01T00:00:00Z
date_updated: 2021-01-12T06:49:09Z
day: '01'
department:
- _id: MiSi
doi: 10.1038/icb.2015.62
intvolume: ' 94'
issue: '1'
language:
- iso: eng
month: '01'
oa_version: None
page: 39 - 51
publication: Immunology and Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '6116'
quality_controlled: '1'
scopus_import: 1
status: public
title: Efficient T-cell priming and activation requires signaling through prostaglandin
E2 (EP) receptors
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 94
year: '2016'
...
---
_id: '1218'
abstract:
- lang: eng
text: Investigating the physiology of cyanobacteria cultured under a diel light
regime is relevant for a better understanding of the resulting growth characteristics
and for specific biotechnological applications that are foreseen for these photosynthetic
organisms. Here, we present the results of a multiomics study of the model cyanobacterium
Synechocystis sp. strain PCC 6803, cultured in a lab-scale photobioreactor in
physiological conditions relevant for large-scale culturing. The culture was sparged
withN2 andCO2, leading to an anoxic environment during the dark period. Growth
followed the availability of light. Metabolite analysis performed with 1Hnuclear
magnetic resonance analysis showed that amino acids involved in nitrogen and sulfur
assimilation showed elevated levels in the light. Most protein levels, analyzed
through mass spectrometry, remained rather stable. However, several high-light-response
proteins and stress-response proteins showed distinct changes at the onset of
the light period. Microarray-based transcript analysis found common patterns of~56%
of the transcriptome following the diel regime. These oscillating transcripts
could be grouped coarsely into genes that were upregulated and downregulated in
the dark period. The accumulated glycogen was degraded in the anaerobic environment
in the dark. A small part was degraded gradually, reflecting basic maintenance
requirements of the cells in darkness. Surprisingly, the largest part was degraded
rapidly in a short time span at the end of the dark period. This degradation could
allow rapid formation of metabolic intermediates at the end of the dark period,
preparing the cells for the resumption of growth at the start of the light period.
acknowledgement: "Dutch Ministry of Economic Affairs, Agriculture, and Innovation
through the program BioSolar CellsS. Andreas Angermayr,Pascal van Alphen, Klaas
J. Hellingwerf\r\nWe thank Naira Quintana (presently at Rousselot, Belgium) for
the ini-\r\ntiative at the 10th Cyanobacterial Molecular Biology Workshop\r\n(CMBW),
June 2010, Lake Arrowhead, Los Angeles, CA, USA, to start the\r\ncollaborative endeavor
reported here. We thank Timo Maarleveld from\r\nCWI/VU (Amsterdam) for a custom-made
Python script handling the output from the NMR analysis and for evaluating and visualizing
the\r\nseparate metabolites for their evaluation. We thank Rob Verpoorte from\r\nLeiden
University (metabolome analysis) and Hans Aerts from the AMC\r\n(proteome analysis)
for lab space and equipment. We thank Robert Leh-\r\nmann (Humboldt University Berlin)
and Ilka Axmann (University of\r\nDüsseldorf) for sharing the R-code for the LOS
transformation of the\r\ntranscript data. We thank Hans C. P. Matthijs from IBED
for inspiring\r\ndialogues and insightful thoughts on continuous culturing of cyanobac-\r\nteria.
We thank Sandra Waaijenborg for performing the transcript nor-\r\nmalization and
Johan Westerhuis from BDA, Jeroen van der Steen and\r\nFilipe Branco dos Santos
from MMP, and Lucas Stal from IBED/NIOZ for\r\nhelpful discussions. We thank Milou
Schuurmans from MMP for help\r\nwith sampling and glycogen determination. We thank
the members of the\r\nRNA Biology & Applied Bioinformatics group at SILS, in particular
Selina\r\nvan Leeuwen, Elisa Hoekstra, and Martijs Jonker, for the microarray anal-\r\nysis.
We thank the reviewers of this work for their insightful comments\r\nwhich improved
the quality of the manuscript. This work, including the efforts of S. Andreas Angermayr,
Pascal van\r\nAlphen, and Klaas J. Hellingwerf, was funded by Dutch Ministry of
Eco-\r\nnomic Affairs, Agriculture, and Innovation through the program BioSolar\r\nCells."
author:
- first_name: Andreas
full_name: Angermayr, Andreas
id: 4677C796-F248-11E8-B48F-1D18A9856A87
last_name: Angermayr
orcid: 0000-0001-8619-2223
- first_name: Pascal
full_name: Van Alphen, Pascal
last_name: Van Alphen
- first_name: Dicle
full_name: Hasdemir, Dicle
last_name: Hasdemir
- first_name: Gertjan
full_name: Kramer, Gertjan
last_name: Kramer
- first_name: Muzamal
full_name: Iqbal, Muzamal
last_name: Iqbal
- first_name: Wilmar
full_name: Van Grondelle, Wilmar
last_name: Van Grondelle
- first_name: Huub
full_name: Hoefsloot, Huub
last_name: Hoefsloot
- first_name: Younghae
full_name: Choi, Younghae
last_name: Choi
- first_name: Klaas
full_name: Hellingwerf, Klaas
last_name: Hellingwerf
citation:
ama: Angermayr A, Van Alphen P, Hasdemir D, et al. Culturing synechocystis sp. Strain
pcc 6803 with N2 and CO2 in a diel regime reveals multiphase glycogen dynamics
with low maintenance costs. Applied and Environmental Microbiology. 2016;82(14):4180-4189.
doi:10.1128/AEM.00256-16
apa: Angermayr, A., Van Alphen, P., Hasdemir, D., Kramer, G., Iqbal, M., Van Grondelle,
W., … Hellingwerf, K. (2016). Culturing synechocystis sp. Strain pcc 6803 with
N2 and CO2 in a diel regime reveals multiphase glycogen dynamics with low maintenance
costs. Applied and Environmental Microbiology. American Society for Microbiology.
https://doi.org/10.1128/AEM.00256-16
chicago: Angermayr, Andreas, Pascal Van Alphen, Dicle Hasdemir, Gertjan Kramer,
Muzamal Iqbal, Wilmar Van Grondelle, Huub Hoefsloot, Younghae Choi, and Klaas
Hellingwerf. “Culturing Synechocystis Sp. Strain Pcc 6803 with N2 and CO2 in a
Diel Regime Reveals Multiphase Glycogen Dynamics with Low Maintenance Costs.”
Applied and Environmental Microbiology. American Society for Microbiology,
2016. https://doi.org/10.1128/AEM.00256-16.
ieee: A. Angermayr et al., “Culturing synechocystis sp. Strain pcc 6803 with
N2 and CO2 in a diel regime reveals multiphase glycogen dynamics with low maintenance
costs,” Applied and Environmental Microbiology, vol. 82, no. 14. American
Society for Microbiology, pp. 4180–4189, 2016.
ista: Angermayr A, Van Alphen P, Hasdemir D, Kramer G, Iqbal M, Van Grondelle W,
Hoefsloot H, Choi Y, Hellingwerf K. 2016. Culturing synechocystis sp. Strain pcc
6803 with N2 and CO2 in a diel regime reveals multiphase glycogen dynamics with
low maintenance costs. Applied and Environmental Microbiology. 82(14), 4180–4189.
mla: Angermayr, Andreas, et al. “Culturing Synechocystis Sp. Strain Pcc 6803 with
N2 and CO2 in a Diel Regime Reveals Multiphase Glycogen Dynamics with Low Maintenance
Costs.” Applied and Environmental Microbiology, vol. 82, no. 14, American
Society for Microbiology, 2016, pp. 4180–89, doi:10.1128/AEM.00256-16.
short: A. Angermayr, P. Van Alphen, D. Hasdemir, G. Kramer, M. Iqbal, W. Van Grondelle,
H. Hoefsloot, Y. Choi, K. Hellingwerf, Applied and Environmental Microbiology
82 (2016) 4180–4189.
date_created: 2018-12-11T11:50:46Z
date_published: 2016-07-01T00:00:00Z
date_updated: 2021-01-12T06:49:10Z
day: '01'
department:
- _id: ToBo
doi: 10.1128/AEM.00256-16
intvolume: ' 82'
issue: '14'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959195/
month: '07'
oa: 1
oa_version: Submitted Version
page: 4180 - 4189
publication: Applied and Environmental Microbiology
publication_status: published
publisher: American Society for Microbiology
publist_id: '6117'
quality_controlled: '1'
scopus_import: 1
status: public
title: Culturing synechocystis sp. Strain pcc 6803 with N2 and CO2 in a diel regime
reveals multiphase glycogen dynamics with low maintenance costs
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 82
year: '2016'
...
---
_id: '1219'
abstract:
- lang: eng
text: We consider N×N random matrices of the form H = W + V where W is a real symmetric
or complex Hermitian Wigner matrix and V is a random or deterministic, real, diagonal
matrix whose entries are independent of W. We assume subexponential decay for
the matrix entries of W, and we choose V so that the eigenvalues ofW and V are
typically of the same order. For a large class of diagonal matrices V , we show
that the local statistics in the bulk of the spectrum are universal in the limit
of large N.
acknowledgement: "J.C. was supported in part by National Research Foundation of Korea
Grant 2011-0013474 and TJ Park Junior Faculty Fellowship.\r\nK.S. was supported
by ERC Advanced Grant RANMAT, No. 338804, and the \"Fund for Math.\"\r\nB.S. was
supported by NSF GRFP Fellowship DGE-1144152.\r\nH.Y. was supported in part by NSF
Grant DMS-13-07444 and Simons investigator fellowship. We thank Paul Bourgade, László
Erd ̋os and Antti Knowles for helpful comments. We are grateful to the Taida Institute
for Mathematical\r\nSciences and National Taiwan Universality for their hospitality
during part of this\r\nresearch. We thank Thomas Spencer and the Institute for Advanced
Study for their\r\nhospitality during the academic year 2013–2014. "
author:
- first_name: Jioon
full_name: Lee, Jioon
last_name: Lee
- first_name: Kevin
full_name: Schnelli, Kevin
id: 434AD0AE-F248-11E8-B48F-1D18A9856A87
last_name: Schnelli
orcid: 0000-0003-0954-3231
- first_name: Ben
full_name: Stetler, Ben
last_name: Stetler
- first_name: Horngtzer
full_name: Yau, Horngtzer
last_name: Yau
citation:
ama: Lee J, Schnelli K, Stetler B, Yau H. Bulk universality for deformed wigner
matrices. Annals of Probability. 2016;44(3):2349-2425. doi:10.1214/15-AOP1023
apa: Lee, J., Schnelli, K., Stetler, B., & Yau, H. (2016). Bulk universality
for deformed wigner matrices. Annals of Probability. Institute of Mathematical
Statistics. https://doi.org/10.1214/15-AOP1023
chicago: Lee, Jioon, Kevin Schnelli, Ben Stetler, and Horngtzer Yau. “Bulk Universality
for Deformed Wigner Matrices.” Annals of Probability. Institute of Mathematical
Statistics, 2016. https://doi.org/10.1214/15-AOP1023.
ieee: J. Lee, K. Schnelli, B. Stetler, and H. Yau, “Bulk universality for deformed
wigner matrices,” Annals of Probability, vol. 44, no. 3. Institute of Mathematical
Statistics, pp. 2349–2425, 2016.
ista: Lee J, Schnelli K, Stetler B, Yau H. 2016. Bulk universality for deformed
wigner matrices. Annals of Probability. 44(3), 2349–2425.
mla: Lee, Jioon, et al. “Bulk Universality for Deformed Wigner Matrices.” Annals
of Probability, vol. 44, no. 3, Institute of Mathematical Statistics, 2016,
pp. 2349–425, doi:10.1214/15-AOP1023.
short: J. Lee, K. Schnelli, B. Stetler, H. Yau, Annals of Probability 44 (2016)
2349–2425.
date_created: 2018-12-11T11:50:47Z
date_published: 2016-01-01T00:00:00Z
date_updated: 2021-01-12T06:49:10Z
day: '01'
department:
- _id: LaEr
doi: 10.1214/15-AOP1023
ec_funded: 1
intvolume: ' 44'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/1405.6634
month: '01'
oa: 1
oa_version: Preprint
page: 2349 - 2425
project:
- _id: 258DCDE6-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '338804'
name: Random matrices, universality and disordered quantum systems
publication: Annals of Probability
publication_status: published
publisher: Institute of Mathematical Statistics
publist_id: '6115'
quality_controlled: '1'
scopus_import: 1
status: public
title: Bulk universality for deformed wigner matrices
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 44
year: '2016'
...
---
_id: '1220'
abstract:
- lang: eng
text: Theoretical and numerical aspects of aerodynamic efficiency of propulsion
systems coupled to the boundary layer of a fuselage are studied. We discuss the
effects of local flow fields, which are affected both by conservative flow acceleration
as well as total pressure losses, on the efficiency of boundary layer immersed
propulsion devices. We introduce the concept of a boundary layer retardation turbine
that helps reduce skin friction over the fuselage. We numerically investigate
efficiency gains offered by boundary layer and wake interacting devices. We discuss
the results in terms of a total energy consumption framework and show that efficiency
gains of any device depend on all the other elements of the propulsion system.
author:
- first_name: Gregor
full_name: Mikić, Gregor
last_name: Mikić
- first_name: Alex
full_name: Stoll, Alex
last_name: Stoll
- first_name: Joe
full_name: Bevirt, Joe
last_name: Bevirt
- first_name: Rok
full_name: Grah, Rok
id: 483E70DE-F248-11E8-B48F-1D18A9856A87
last_name: Grah
orcid: 0000-0003-2539-3560
- first_name: Mark
full_name: Moore, Mark
last_name: Moore
citation:
ama: 'Mikić G, Stoll A, Bevirt J, Grah R, Moore M. Fuselage boundary layer ingestion
propulsion applied to a thin haul commuter aircraft for optimal efficiency. In:
AIAA; 2016:1-19. doi:10.2514/6.2016-3764'
apa: 'Mikić, G., Stoll, A., Bevirt, J., Grah, R., & Moore, M. (2016). Fuselage
boundary layer ingestion propulsion applied to a thin haul commuter aircraft for
optimal efficiency (pp. 1–19). Presented at the AIAA: Aviation Technology, Integration,
and Operations Conference, Washington, D.C., USA: AIAA. https://doi.org/10.2514/6.2016-3764'
chicago: Mikić, Gregor, Alex Stoll, Joe Bevirt, Rok Grah, and Mark Moore. “Fuselage
Boundary Layer Ingestion Propulsion Applied to a Thin Haul Commuter Aircraft for
Optimal Efficiency,” 1–19. AIAA, 2016. https://doi.org/10.2514/6.2016-3764.
ieee: 'G. Mikić, A. Stoll, J. Bevirt, R. Grah, and M. Moore, “Fuselage boundary
layer ingestion propulsion applied to a thin haul commuter aircraft for optimal
efficiency,” presented at the AIAA: Aviation Technology, Integration, and Operations
Conference, Washington, D.C., USA, 2016, pp. 1–19.'
ista: 'Mikić G, Stoll A, Bevirt J, Grah R, Moore M. 2016. Fuselage boundary layer
ingestion propulsion applied to a thin haul commuter aircraft for optimal efficiency.
AIAA: Aviation Technology, Integration, and Operations Conference, 1–19.'
mla: Mikić, Gregor, et al. Fuselage Boundary Layer Ingestion Propulsion Applied
to a Thin Haul Commuter Aircraft for Optimal Efficiency. AIAA, 2016, pp. 1–19,
doi:10.2514/6.2016-3764.
short: G. Mikić, A. Stoll, J. Bevirt, R. Grah, M. Moore, in:, AIAA, 2016, pp. 1–19.
conference:
end_date: 2016-06-17
location: Washington, D.C., USA
name: 'AIAA: Aviation Technology, Integration, and Operations Conference'
start_date: 2016-06-13
date_created: 2018-12-11T11:50:47Z
date_published: 2016-06-01T00:00:00Z
date_updated: 2023-02-21T10:17:50Z
day: '01'
department:
- _id: CaGu
- _id: GaTk
doi: 10.2514/6.2016-3764
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://ntrs.nasa.gov/search.jsp?R=20160010167&hterms=Fuselage+boundary+layer+ingestion+propulsion+applied+thin+haul+commuter+aircraft+optimal+efficiency&qs=N%3D0%26Ntk%3DAll%26Ntt%3DFuselage%2520boundary%2520layer%2520ingestion%2520propulsion%2520applied%2520to%2520a%2520thin%2520haul%2520commuter%2520aircraft%2520for%2520optimal%2520efficiency%26Ntx%3Dmode%2520matchallpartial%26Nm%3D123%7CCollection%7CNASA%2520STI%7C%7C17%7CCollection%7CNACA
month: '06'
oa: 1
oa_version: Preprint
page: 1 - 19
publication_status: published
publisher: AIAA
publist_id: '6114'
quality_controlled: '1'
scopus_import: 1
status: public
title: Fuselage boundary layer ingestion propulsion applied to a thin haul commuter
aircraft for optimal efficiency
type: conference
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
year: '2016'
...
---
_id: '1221'
abstract:
- lang: eng
text: The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in
plants. Since decades ago, it has been the prime receptor candidate for the plant
hormone auxin with a plethora of described functions in auxin signaling and development.
The developmental importance of ABP1 has recently been questioned by identification
of Arabidopsis thaliana abp1 knock-out alleles that show no obvious phenotypes
under normal growth conditions. In this study, we examined the contradiction between
the normal growth and development of the abp1 knock-outs and the strong morphological
defects observed in three different ethanol-inducible abp1 knock-down mutants
( abp1-AS, SS12K, SS12S). By analyzing segregating populations of abp1 knock-out
vs. abp1 knock-down crosses we show that the strong morphological defects that
were believed to be the result of conditional down-regulation of ABP1 can be reproduced
also in the absence of the functional ABP1 protein. This data suggests that the
phenotypes in abp1 knock-down lines are due to the off-target effects and asks
for further reflections on the biological function of ABP1 or alternative explanations
for the missing phenotypic defects in the abp1 loss-of-function alleles.
acknowledgement: "This work was supported by ERC Independent Research grant (ERC-2011-StG-20101109-PSDP
to JF). JM internship was supported by the grant “Action Austria – Slovakia”. MG
was supported by the scholarship \"Stipendien der Stipendienstiftung der Republik
Österreich\". Work by EH and CPR were supported by ANR blanc ANR-14-CE11-0018. We
would like to thank Mark Estelle and Yunde Zhao for provid\r\n-\r\ning \r\nabp1-c1\r\n,
\r\nabp1-TD1 \r\nand \r\nabp1-WTc1 \r\nseeds. We thank Emeline \r\nHuault for technical
assistance."
article_number: '86'
article_processing_charge: No
article_type: original
author:
- first_name: Jaroslav
full_name: Michalko, Jaroslav
id: 483727CA-F248-11E8-B48F-1D18A9856A87
last_name: Michalko
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Catherine
full_name: Perrot Rechenmann, Catherine
last_name: Perrot Rechenmann
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Michalko J, Glanc M, Perrot Rechenmann C, Friml J. Strong morphological defects
in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional
ABP1 protein. F1000 Research . 2016;5. doi:10.12688/f1000research.7654.1
apa: Michalko, J., Glanc, M., Perrot Rechenmann, C., & Friml, J. (2016). Strong
morphological defects in conditional Arabidopsis abp1 knock-down mutants generated
in absence of functional ABP1 protein. F1000 Research . F1000 Research.
https://doi.org/10.12688/f1000research.7654.1
chicago: Michalko, Jaroslav, Matous Glanc, Catherine Perrot Rechenmann, and Jiří
Friml. “Strong Morphological Defects in Conditional Arabidopsis Abp1 Knock-down
Mutants Generated in Absence of Functional ABP1 Protein.” F1000 Research .
F1000 Research, 2016. https://doi.org/10.12688/f1000research.7654.1.
ieee: J. Michalko, M. Glanc, C. Perrot Rechenmann, and J. Friml, “Strong morphological
defects in conditional Arabidopsis abp1 knock-down mutants generated in absence
of functional ABP1 protein,” F1000 Research , vol. 5. F1000 Research, 2016.
ista: Michalko J, Glanc M, Perrot Rechenmann C, Friml J. 2016. Strong morphological
defects in conditional Arabidopsis abp1 knock-down mutants generated in absence
of functional ABP1 protein. F1000 Research . 5, 86.
mla: Michalko, Jaroslav, et al. “Strong Morphological Defects in Conditional Arabidopsis
Abp1 Knock-down Mutants Generated in Absence of Functional ABP1 Protein.” F1000
Research , vol. 5, 86, F1000 Research, 2016, doi:10.12688/f1000research.7654.1.
short: J. Michalko, M. Glanc, C. Perrot Rechenmann, J. Friml, F1000 Research 5
(2016).
date_created: 2018-12-11T11:50:47Z
date_published: 2016-01-20T00:00:00Z
date_updated: 2022-03-24T09:12:49Z
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title: Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants
generated in absence of functional ABP1 protein
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