[{"date_updated":"2023-09-07T12:39:44Z","date_created":"2018-12-11T11:45:49Z","author":[{"full_name":"Leithner, Alexander F","first_name":"Alexander F","last_name":"Leithner","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1073-744X"}],"related_material":{"record":[{"id":"1321","relation":"part_of_dissertation","status":"public"}]},"publication_status":"published","department":[{"_id":"MiSi"}],"publisher":"Institute of Science and Technology Austria","acknowledgement":"First of all I would like to thank Michael Sixt for giving me the opportunity to work in \r\nhis group and for his support throughout the years. He is a truly inspiring person and \r\nthe best boss one can imagine. I would also like to thank all current and past \r\nmembers of the Sixt group for their help and the great working atmosphere in the lab. \r\nIt is a true privilege to work with such a bright, funny and friendly group of people and \r\nI’m proud that I could be part of it. Furthermore, I would like to say ‘thank you’ to Daria Siekhaus for all the meetings and discussion we had throughout the years \r\nand to Federica Benvenuti for being part of my committee. I am also grateful to Jack \r\nMerrin in the nanofabrication facility and all the people working in the bioimaging-\r\n, the electron microscopy- and the preclinical facilities.","year":"2018","file_date_updated":"2021-02-11T23:30:17Z","publist_id":"7542","acknowledged_ssus":[{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"EM-Fac"}],"degree_awarded":"PhD","supervisor":[{"full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","last_name":"Sixt"}],"language":[{"iso":"eng"}],"doi":"10.15479/AT:ISTA:th_998","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"month":"04","publication_identifier":{"issn":["2663-337X"]},"oa_version":"Published Version","file":[{"checksum":"d5e3edbac548c26c1fa43a4b37a54a4c","date_created":"2019-04-05T09:23:11Z","date_updated":"2021-02-11T23:30:17Z","file_id":"6219","relation":"source_file","creator":"dernst","file_size":29027671,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","access_level":"closed","file_name":"PhD_thesis_AlexLeithner_final_version.docx","embargo_to":"open_access"},{"content_type":"application/pdf","file_size":66045341,"creator":"dernst","access_level":"open_access","file_name":"PhD_thesis_AlexLeithner.pdf","checksum":"071f7476db29e41146824ebd0697cb10","date_updated":"2021-02-11T11:17:16Z","date_created":"2019-04-05T09:23:11Z","relation":"main_file","file_id":"6220","embargo":"2019-04-15"}],"pubrep_id":"998","status":"public","ddc":["571","599","610"],"title":"Branched actin networks in dendritic cell biology","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"323","abstract":[{"lang":"eng","text":"In the here presented thesis, we explore the role of branched actin networks in cell migration and antigen presentation, the two most relevant processes in dendritic cell biology. Branched actin networks construct lamellipodial protrusions at the leading edge of migrating cells. These are typically seen as adhesive structures, which mediate force transduction to the extracellular matrix that leads to forward locomotion. We ablated Arp2/3 nucleation promoting factor WAVE in DCs and found that the resulting cells lack lamellipodial protrusions. Instead, depending on the maturation state, one or multiple filopodia were formed. By challenging these cells in a variety of migration assays we found that lamellipodial protrusions are dispensable for the locomotion of leukocytes and actually dampen the speed of migration. However, lamellipodia are critically required to negotiate complex environments that DCs experience while they travel to the next draining lymph node. Taken together our results suggest that leukocyte lamellipodia have rather a sensory- than a force transducing function. Furthermore, we show for the first time structure and dynamics of dendritic cell F-actin at the immunological synapse with naïve T cells. Dendritic cell F-actin appears as dynamic foci that are nucleated by the Arp2/3 complex. WAVE ablated dendritic cells show increased membrane tension, leading to an altered ultrastructure of the immunological synapse and severe T cell priming defects. These results point towards a previously unappreciated role of the cellular mechanics of dendritic cells in T cell activation. Additionally, we present a novel cell culture based system for the differentiation of dendritic cells from conditionally immortalized hematopoietic precursors. These precursor cells are genetically tractable via the CRISPR/Cas9 system while they retain their ability to differentiate into highly migratory dendritic cells and other immune cells. This will foster the study of all aspects of dendritic cell biology and beyond. "}],"alternative_title":["ISTA Thesis"],"type":"dissertation","date_published":"2018-04-12T00:00:00Z","page":"99","citation":{"ama":"Leithner AF. Branched actin networks in dendritic cell biology. 2018. doi:10.15479/AT:ISTA:th_998","ista":"Leithner AF. 2018. Branched actin networks in dendritic cell biology. Institute of Science and Technology Austria.","apa":"Leithner, A. F. (2018). Branched actin networks in dendritic cell biology. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_998","ieee":"A. F. Leithner, “Branched actin networks in dendritic cell biology,” Institute of Science and Technology Austria, 2018.","mla":"Leithner, Alexander F. Branched Actin Networks in Dendritic Cell Biology. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_998.","short":"A.F. Leithner, Branched Actin Networks in Dendritic Cell Biology, Institute of Science and Technology Austria, 2018.","chicago":"Leithner, Alexander F. “Branched Actin Networks in Dendritic Cell Biology.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_998."},"day":"12","article_processing_charge":"No","has_accepted_license":"1"},{"_id":"539","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","title":"Identification and characterization of novel auxin-cytokinin cross-talk components","ddc":["570"],"pubrep_id":"930","file":[{"date_updated":"2020-12-02T23:30:08Z","date_created":"2019-04-05T09:37:56Z","checksum":"0c9d6d1c80d9857e6e545213467bbcb2","file_id":"6226","relation":"source_file","creator":"dernst","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_size":28112114,"file_name":"2018_Hurny_thesis_source.docx","embargo_to":"open_access","access_level":"closed"},{"content_type":"application/pdf","file_size":12524427,"creator":"dernst","access_level":"open_access","file_name":"2018_Hurny_thesis.pdf","checksum":"ecbe481a1413d270bd501b872c7ed54f","date_updated":"2020-12-02T09:52:16Z","date_created":"2019-04-05T09:37:55Z","relation":"main_file","embargo":"2019-07-10","file_id":"6227"}],"oa_version":"Published Version","type":"dissertation","alternative_title":["ISTA Thesis"],"abstract":[{"text":"The whole life cycle of plants as well as their responses to environmental stimuli is governed by a complex network of hormonal regulations. A number of studies have demonstrated an essential role of both auxin and cytokinin in the regulation of many aspects of plant growth and development including embryogenesis, postembryonic organogenic processes such as root, and shoot branching, root and shoot apical meristem activity and phyllotaxis. Over the last decades essential knowledge on the key molecular factors and pathways that spatio-temporally define auxin and cytokinin activities in the plant body has accumulated. However, how both hormonal pathways are interconnected by a complex network of interactions and feedback circuits that determines the final outcome of the individual hormone actions is still largely unknown. Root system architecture establishment and in particular formation of lateral organs is prime example of developmental process at whose regulation both auxin and cytokinin pathways converge. To dissect convergence points and pathways that tightly balance auxin - cytokinin antagonistic activities that determine the root branching pattern transcriptome profiling was applied. Genome wide expression analyses of the xylem pole pericycle, a tissue giving rise to lateral roots, led to identification of genes that are highly responsive to combinatorial auxin and cytokinin treatments and play an essential function in the auxin-cytokinin regulated root branching. SYNERGISTIC AUXIN CYTOKININ 1 (SYAC1) gene, which encodes for a protein of unknown function, was detected among the top candidate genes of which expression was synergistically up-regulated by simultaneous hormonal treatment. Plants with modulated SYAC1 activity exhibit severe defects in the root system establishment and attenuate developmental responses to both auxin and cytokinin. To explore the biological function of the SYAC1, we employed different strategies including expression pattern analysis, subcellular localization and phenotypic analyses of the syac1 loss-of-function and gain-of-function transgenic lines along with the identification of the SYAC1 interaction partners. Detailed functional characterization revealed that SYAC1 acts as a developmentally specific regulator of the secretory pathway to control deposition of cell wall components and thereby rapidly fine tune elongation growth.","lang":"eng"}],"citation":{"apa":"Hurny, A. (2018). Identification and characterization of novel auxin-cytokinin cross-talk components. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_930","ieee":"A. Hurny, “Identification and characterization of novel auxin-cytokinin cross-talk components,” Institute of Science and Technology Austria, 2018.","ista":"Hurny A. 2018. Identification and characterization of novel auxin-cytokinin cross-talk components. Institute of Science and Technology Austria.","ama":"Hurny A. Identification and characterization of novel auxin-cytokinin cross-talk components. 2018. doi:10.15479/AT:ISTA:th_930","chicago":"Hurny, Andrej. “Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_930.","short":"A. Hurny, Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components, Institute of Science and Technology Austria, 2018.","mla":"Hurny, Andrej. Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_930."},"page":"147","date_published":"2018-01-01T00:00:00Z","day":"01","has_accepted_license":"1","article_processing_charge":"No","year":"2018","publication_status":"published","publisher":"Institute of Science and Technology Austria","department":[{"_id":"EvBe"}],"author":[{"id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3638-1426","first_name":"Andrej","last_name":"Hurny","full_name":"Hurny, Andrej"}],"related_material":{"record":[{"id":"1024","relation":"part_of_dissertation","status":"public"}]},"date_created":"2018-12-11T11:47:03Z","date_updated":"2023-09-07T12:41:06Z","file_date_updated":"2020-12-02T23:30:08Z","publist_id":"7277","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"doi":"10.15479/AT:ISTA:th_930","degree_awarded":"PhD","supervisor":[{"last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva"}],"language":[{"iso":"eng"}],"month":"01","publication_identifier":{"issn":["2663-337X"]}},{"abstract":[{"lang":"eng","text":"The hippocampus is a key brain region for spatial memory and navigation and is needed at all stages of memory, including encoding, consolidation, and recall. Hippocampal place cells selectively discharge at specific locations of the environment to form a cognitive map of the space. During the rest period and sleep following spatial navigation and/or learning, the waking activity of the place cells is reactivated within high synchrony events. This reactivation is thought to be important for memory consolidation and stabilization of the spatial representations. The aim of my thesis was to directly test whether the reactivation content encoded in firing patterns of place cells is important for consolidation of spatial memories. In particular, I aimed to test whether, in cases when multiple spatial memory traces are acquired during learning, the specific disruption of the reactivation of a subset of these memories leads to the selective disruption of the corresponding memory traces or through memory interference the other learned memories are disrupted as well. In this thesis, using a modified cheeseboard paradigm and a closed-loop recording setup with feedback optogenetic stimulation, I examined how the disruption of the reactivation of specific spiking patterns affects consolidation of the corresponding memory traces. To obtain multiple distinctive memories, animals had to perform a spatial task in two distinct cheeseboard environments and the reactivation of spiking patterns associated with one of the environments (target) was disrupted after learning during four hours rest period using a real-time decoding method. This real-time decoding method was capable of selectively affecting the firing rates and cofiring correlations of the target environment-encoding cells. The selective disruption led to behavioural impairment in the memory tests after the rest periods in the target environment but not in the other undisrupted control environment. In addition, the map of the target environment was less stable in the impaired memory tests compared to the learning session before than the map of the control environment. However, when the animal relearned the task, the same map recurred in the target environment that was present during learning before the disruption. Altogether my work demonstrated that the reactivation content is important: assembly-related disruption of reactivation can lead to a selective memory impairment and deficiency in map stability. These findings indeed suggest that reactivated assembly patterns reflect processes associated with the consolidation of memory traces. "}],"type":"dissertation","alternative_title":["ISTA Thesis"],"pubrep_id":"1042","oa_version":"Published Version","file":[{"file_size":7666687,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","creator":"dernst","embargo_to":"open_access","file_name":"2018_Thesis_Gridchyn_source.docx","access_level":"closed","date_updated":"2021-02-11T23:30:22Z","date_created":"2019-04-08T13:36:01Z","checksum":"7db4415e435590fa33542c7b0a0321d7","relation":"source_file","file_id":"6236"},{"relation":"main_file","file_id":"6237","embargo":"2019-08-29","date_updated":"2021-02-11T11:17:18Z","date_created":"2019-04-08T13:36:01Z","checksum":"f96f3fe8979f7b1e6db6acaca962b10c","file_name":"2018_Thesis_Gridchyn.pdf","access_level":"open_access","content_type":"application/pdf","file_size":6034153,"creator":"dernst"}],"_id":"48","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","ddc":["573"],"title":"Reactivation content is important for consolidation of spatial memory","status":"public","day":"27","article_processing_charge":"No","has_accepted_license":"1","date_published":"2018-08-27T00:00:00Z","citation":{"mla":"Gridchyn, Igor. Reactivation Content Is Important for Consolidation of Spatial Memory. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th_1042.","short":"I. Gridchyn, Reactivation Content Is Important for Consolidation of Spatial Memory, Institute of Science and Technology Austria, 2018.","chicago":"Gridchyn, Igor. “Reactivation Content Is Important for Consolidation of Spatial Memory.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_1042.","ama":"Gridchyn I. Reactivation content is important for consolidation of spatial memory. 2018. doi:10.15479/AT:ISTA:th_1042","ista":"Gridchyn I. 2018. Reactivation content is important for consolidation of spatial memory. Institute of Science and Technology Austria.","ieee":"I. Gridchyn, “Reactivation content is important for consolidation of spatial memory,” Institute of Science and Technology Austria, 2018.","apa":"Gridchyn, I. (2018). Reactivation content is important for consolidation of spatial memory. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_1042"},"page":"104","file_date_updated":"2021-02-11T23:30:22Z","publist_id":"8006","author":[{"full_name":"Gridchyn, Igor","first_name":"Igor","last_name":"Gridchyn","id":"4B60654C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1807-1929"}],"date_created":"2018-12-11T11:44:21Z","date_updated":"2023-09-07T12:42:44Z","year":"2018","publication_status":"published","publisher":"Institute of Science and Technology Austria","department":[{"_id":"JoCs"}],"month":"08","publication_identifier":{"issn":["2663-337X"]},"doi":"10.15479/AT:ISTA:th_1042","supervisor":[{"first_name":"Jozsef L","last_name":"Csicsvari","id":"3FA14672-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5193-4036","full_name":"Csicsvari, Jozsef L"}],"degree_awarded":"PhD","language":[{"iso":"eng"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"}},{"month":"07","publication_identifier":{"issn":["2663-337X"]},"degree_awarded":"PhD","supervisor":[{"full_name":"Siekhaus, Daria E","last_name":"Siekhaus","first_name":"Daria E","orcid":"0000-0001-8323-8353","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87"}],"language":[{"iso":"eng"}],"doi":"10.15479/AT:ISTA:th1064","oa":1,"file_date_updated":"2021-02-11T11:17:16Z","publist_id":"8047","date_created":"2018-12-11T11:44:08Z","date_updated":"2023-09-07T12:43:10Z","author":[{"full_name":"Belyaeva, Vera","id":"47F080FE-F248-11E8-B48F-1D18A9856A87","last_name":"Belyaeva","first_name":"Vera"}],"publication_status":"published","publisher":"Institute of Science and Technology Austria","department":[{"_id":"DaSi"}],"year":"2018","day":"01","article_processing_charge":"No","has_accepted_license":"1","date_published":"2018-07-01T00:00:00Z","page":"96","citation":{"chicago":"Belyaeva, Vera. “Transcriptional Regulation of Macrophage Migration in the Drosophila Melanogaster Embryo .” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th1064.","short":"V. Belyaeva, Transcriptional Regulation of Macrophage Migration in the Drosophila Melanogaster Embryo , Institute of Science and Technology Austria, 2018.","mla":"Belyaeva, Vera. Transcriptional Regulation of Macrophage Migration in the Drosophila Melanogaster Embryo . Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:th1064.","ieee":"V. Belyaeva, “Transcriptional regulation of macrophage migration in the Drosophila melanogaster embryo ,” Institute of Science and Technology Austria, 2018.","apa":"Belyaeva, V. (2018). Transcriptional regulation of macrophage migration in the Drosophila melanogaster embryo . Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th1064","ista":"Belyaeva V. 2018. Transcriptional regulation of macrophage migration in the Drosophila melanogaster embryo . Institute of Science and Technology Austria.","ama":"Belyaeva V. Transcriptional regulation of macrophage migration in the Drosophila melanogaster embryo . 2018. doi:10.15479/AT:ISTA:th1064"},"abstract":[{"text":"Immune cells migrating to the sites of infection navigate through diverse tissue architectures and switch their migratory mechanisms upon demand. However, little is known about systemic regulators that could allow the acquisition of these mechanisms. We performed a genetic screen in Drosophila melanogaster to identify regulators of germband invasion by embryonic macrophages into the confined space between the ectoderm and mesoderm. We have found that bZIP circadian transcription factors (TFs) Kayak (dFos) and Vrille (dNFIL3) have opposite effects on macrophage germband infiltration: Kayak facilitated and Vrille inhibited it. These TFs are enriched in the macrophages during migration and genetically interact to control it. Kayak sets a less coordinated mode of migration of the macrophage group and increases the probability and length of Levy walks. Intriguingly, the motility of kayak mutant macrophages was also strongly affected during initial germband invasion but not along another less confined route. Inhibiting Rho1 signaling within the tail ectoderm partially rescued the Kayak mutant phenotype, strongly suggesting that migrating macrophages have to overcome a barrier imposed by the stiffness of the ectoderm. Also, Kayak appeared to be important for the maintenance of the round cell shape and the rear edge translocation of the macrophages invading the germband. Complementary to this, the cortical actin cytoskeleton of Kayak- deficient macrophages was strongly affected. RNA sequencing revealed the filamin Cheerio and tetraspanin TM4SF to be downstream of Kayak. Chromatin immunoprecipitation and immunostaining revealed that the formin Diaphanous is another downstream target of Kayak. Immunostaining revealed that the formin Diaphanous is another downstream target of Kayak. Indeed, Cheerio, TM4SF and Diaphanous are required within macrophages for germband invasion, and expression of constitutively active Diaphanous in macrophages was able to rescue the kayak mutant phenotype. Moreover, Cher and Diaphanous are also reduced in the macrophages overexpressing Vrille. We hypothesize that Kayak, through its targets, increases actin polymerization and cortical tension in macrophages and thus allows extra force generation necessary for macrophage dissemination and migration through confined stiff tissues, while Vrille counterbalances it.","lang":"eng"}],"alternative_title":["ISTA Thesis"],"type":"dissertation","file":[{"date_updated":"2020-07-14T12:48:14Z","date_created":"2019-04-08T14:13:12Z","checksum":"d27b2465cb70d0c9678a0381b9b6ced1","relation":"source_file","file_id":"6243","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_size":102737483,"creator":"dernst","embargo_to":"open_access","file_name":"2018_Thesis_Belyaeva_source.docx","access_level":"closed"},{"date_updated":"2021-02-11T11:17:16Z","date_created":"2019-04-08T14:14:08Z","checksum":"a2939b61bde2de7b8ced77bbae0eaaed","relation":"main_file","file_id":"6244","embargo":"2019-11-19","content_type":"application/pdf","file_size":88077843,"creator":"dernst","file_name":"2018_Thesis_Belyaeva.pdf","access_level":"open_access"}],"oa_version":"Published Version","pubrep_id":"1064","ddc":["570"],"title":"Transcriptional regulation of macrophage migration in the Drosophila melanogaster embryo ","status":"public","_id":"9","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1"},{"alternative_title":["ISTA Thesis"],"type":"dissertation","abstract":[{"text":"A major challenge in neuroscience research is to dissect the circuits that orchestrate behavior in health and disease. Proteins from a wide range of non-mammalian species, such as microbial opsins, have been successfully transplanted to specific neuronal targets to override their natural communication patterns. The goal of our work is to manipulate synaptic communication in a manner that closely incorporates the functional intricacies of synapses by preserving temporal encoding (i.e. the firing pattern of the presynaptic neuron) and connectivity (i.e. target specific synapses rather than specific neurons). Our strategy to achieve this goal builds on the use of non-mammalian transplants to create a synthetic synapse. The mode of modulation comes from pre-synaptic uptake of a synthetic neurotransmitter (SN) into synaptic vesicles by means of a genetically targeted transporter selective for the SN. Upon natural vesicular release, exposure of the SN to the synaptic cleft will modify the post-synaptic potential through an orthogonal ligand gated ion channel. To achieve this goal we have functionally characterized a mixed cationic methionine-gated ion channel from Arabidopsis thaliana, designed a method to functionally characterize a synthetic transporter in isolated synaptic vesicles without the need for transgenic animals, identified and extracted multiple prokaryotic uptake systems that are substrate specific for methionine (Met), and established a primary/cell line co-culture system that would allow future combinatorial testing of this orthogonal transmitter-transporter-channel trifecta. Synthetic synapses will provide a unique opportunity to manipulate synaptic communication while maintaining the electrophysiological integrity of the pre-synaptic cell. In this way, information may be preserved that was generated in upstream circuits and that could be essential for concerted function and information processing. ","lang":"eng"}],"ddc":["571","573"],"title":"Design and characterization of methods and biological components to realize synthetic neurotransmission ","status":"public","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"6266","file":[{"date_updated":"2021-02-11T11:17:16Z","date_created":"2019-04-09T14:12:40Z","checksum":"9d2c2dca04b00e485470c28b262af59a","file_id":"6267","embargo":"2019-11-24","relation":"main_file","creator":"dernst","file_size":4906420,"content_type":"application/pdf","file_name":"2018_Thesis_McKenzie.pdf","access_level":"open_access"},{"file_id":"6268","relation":"source_file","date_updated":"2020-07-14T12:47:25Z","date_created":"2019-04-09T14:12:40Z","checksum":"50b58c272899601bc6fd9642c4dc97f1","file_name":"2018_Thesis_McKenzie_source.docx","embargo_to":"open_access","access_level":"closed","creator":"dernst","file_size":5053545,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document"}],"oa_version":"Published Version","pubrep_id":"1055","article_processing_charge":"No","has_accepted_license":"1","day":"31","page":"95","citation":{"apa":"Mckenzie, C. (2018). Design and characterization of methods and biological components to realize synthetic neurotransmission . Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:th_1055","ieee":"C. Mckenzie, “Design and characterization of methods and biological components to realize synthetic neurotransmission ,” Institute of Science and Technology Austria, 2018.","ista":"Mckenzie C. 2018. Design and characterization of methods and biological components to realize synthetic neurotransmission . Institute of Science and Technology Austria.","ama":"Mckenzie C. Design and characterization of methods and biological components to realize synthetic neurotransmission . 2018. doi:10.15479/at:ista:th_1055","chicago":"Mckenzie, Catherine. “Design and Characterization of Methods and Biological Components to Realize Synthetic Neurotransmission .” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/at:ista:th_1055.","short":"C. Mckenzie, Design and Characterization of Methods and Biological Components to Realize Synthetic Neurotransmission , Institute of Science and Technology Austria, 2018.","mla":"Mckenzie, Catherine. Design and Characterization of Methods and Biological Components to Realize Synthetic Neurotransmission . Institute of Science and Technology Austria, 2018, doi:10.15479/at:ista:th_1055."},"date_published":"2018-10-31T00:00:00Z","file_date_updated":"2021-02-11T11:17:16Z","department":[{"_id":"HaJa"}],"publisher":"Institute of Science and Technology Austria","publication_status":"published","year":"2018","date_created":"2019-04-09T14:13:39Z","date_updated":"2023-09-07T13:02:37Z","related_material":{"record":[{"id":"7132","relation":"new_edition","status":"public"}]},"author":[{"last_name":"Mckenzie","first_name":"Catherine","id":"3EEDE19A-F248-11E8-B48F-1D18A9856A87","full_name":"Mckenzie, Catherine"}],"publication_identifier":{"issn":["2663-337X"]},"month":"10","oa":1,"language":[{"iso":"eng"}],"degree_awarded":"PhD","supervisor":[{"first_name":"Harald L","last_name":"Janovjak","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8023-9315","full_name":"Janovjak, Harald L"}],"doi":"10.15479/at:ista:th_1055"}]