---
_id: '8930'
abstract:
- lang: eng
text: Phenomenological relations such as Ohm’s or Fourier’s law have a venerable
history in physics but are still scarce in biology. This situation restrains predictive
theory. Here, we build on bacterial “growth laws,” which capture physiological
feedback between translation and cell growth, to construct a minimal biophysical
model for the combined action of ribosome-targeting antibiotics. Our model predicts
drug interactions like antagonism or synergy solely from responses to individual
drugs. We provide analytical results for limiting cases, which agree well with
numerical results. We systematically refine the model by including direct physical
interactions of different antibiotics on the ribosome. In a limiting case, our
model provides a mechanistic underpinning for recent predictions of higher-order
interactions that were derived using entropy maximization. We further refine the
model to include the effects of antibiotics that mimic starvation and the presence
of resistance genes. We describe the impact of a starvation-mimicking antibiotic
on drug interactions analytically and verify it experimentally. Our extended model
suggests a change in the type of drug interaction that depends on the strength
of resistance, which challenges established rescaling paradigms. We experimentally
show that the presence of unregulated resistance genes can lead to altered drug
interaction, which agrees with the prediction of the model. While minimal, the
model is readily adaptable and opens the door to predicting interactions of second
and higher-order in a broad range of biological systems.
article_processing_charge: No
author:
- first_name: Bor
full_name: Kavcic, Bor
id: 350F91D2-F248-11E8-B48F-1D18A9856A87
last_name: Kavcic
orcid: 0000-0001-6041-254X
citation:
ama: Kavcic B. Analysis scripts and research data for the paper “Minimal biophysical
model of combined antibiotic action.” 2020. doi:10.15479/AT:ISTA:8930
apa: Kavcic, B. (2020). Analysis scripts and research data for the paper “Minimal
biophysical model of combined antibiotic action.” Institute of Science and Technology
Austria. https://doi.org/10.15479/AT:ISTA:8930
chicago: Kavcic, Bor. “Analysis Scripts and Research Data for the Paper ‘Minimal
Biophysical Model of Combined Antibiotic Action.’” Institute of Science and Technology
Austria, 2020. https://doi.org/10.15479/AT:ISTA:8930.
ieee: B. Kavcic, “Analysis scripts and research data for the paper ‘Minimal biophysical
model of combined antibiotic action.’” Institute of Science and Technology Austria,
2020.
ista: Kavcic B. 2020. Analysis scripts and research data for the paper ‘Minimal
biophysical model of combined antibiotic action’, Institute of Science and Technology
Austria, 10.15479/AT:ISTA:8930.
mla: Kavcic, Bor. Analysis Scripts and Research Data for the Paper “Minimal Biophysical
Model of Combined Antibiotic Action.” Institute of Science and Technology
Austria, 2020, doi:10.15479/AT:ISTA:8930.
short: B. Kavcic, (2020).
contributor:
- contributor_type: supervisor
first_name: Gašper
id: 3D494DCA-F248-11E8-B48F-1D18A9856A87
last_name: Tkačik
orcid: 0000-0002-6699-1455
- contributor_type: supervisor
first_name: Tobias
id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
last_name: Bollenbach
date_created: 2020-12-09T15:04:02Z
date_published: 2020-12-10T00:00:00Z
date_updated: 2024-02-21T12:41:42Z
day: '10'
ddc:
- '570'
department:
- _id: GaTk
doi: 10.15479/AT:ISTA:8930
file:
- access_level: open_access
checksum: 60a818edeffaa7da1ebf5f8fbea9ba18
content_type: application/zip
creator: bkavcic
date_created: 2020-12-09T15:00:19Z
date_updated: 2020-12-09T15:00:19Z
file_id: '8932'
file_name: PLoSCompBiol2020_datarep.zip
file_size: 315494370
relation: main_file
success: 1
file_date_updated: 2020-12-09T15:00:19Z
has_accepted_license: '1'
keyword:
- Escherichia coli
- antibiotic combinations
- translation
- growth laws
- drug interactions
- bacterial physiology
- translation inhibitors
month: '12'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '8997'
relation: used_in_publication
status: public
status: public
title: Analysis scripts and research data for the paper "Minimal biophysical model
of combined antibiotic action"
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '8951'
abstract:
- lang: eng
text: Gene expression levels are influenced by multiple coexisting molecular mechanisms.
Some of these interactions, such as those of transcription factors and promoters
have been studied extensively. However, predicting phenotypes of gene regulatory
networks remains a major challenge. Here, we use a well-defined synthetic gene
regulatory network to study how network phenotypes depend on local genetic context,
i.e. the genetic neighborhood of a transcription factor and its relative position.
We show that one gene regulatory network with fixed topology can display not only
quantitatively but also qualitatively different phenotypes, depending solely on
the local genetic context of its components. Our results demonstrate that changes
in local genetic context can place a single transcriptional unit within two separate
regulons without the need for complex regulatory sequences. We propose that relative
order of individual transcriptional units, with its potential for combinatorial
complexity, plays an important role in shaping phenotypes of gene regulatory networks.
article_processing_charge: No
author:
- first_name: Anna A
full_name: Nagy-Staron, Anna A
id: 3ABC5BA6-F248-11E8-B48F-1D18A9856A87
last_name: Nagy-Staron
orcid: 0000-0002-1391-8377
citation:
ama: Nagy-Staron AA. Sequences of gene regulatory network permutations for the article
“Local genetic context shapes the function of a gene regulatory network.” 2020.
doi:10.15479/AT:ISTA:8951
apa: Nagy-Staron, A. A. (2020). Sequences of gene regulatory network permutations
for the article “Local genetic context shapes the function of a gene regulatory
network.” Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:8951
chicago: Nagy-Staron, Anna A. “Sequences of Gene Regulatory Network Permutations
for the Article ‘Local Genetic Context Shapes the Function of a Gene Regulatory
Network.’” Institute of Science and Technology Austria, 2020. https://doi.org/10.15479/AT:ISTA:8951.
ieee: A. A. Nagy-Staron, “Sequences of gene regulatory network permutations for
the article ‘Local genetic context shapes the function of a gene regulatory network.’”
Institute of Science and Technology Austria, 2020.
ista: Nagy-Staron AA. 2020. Sequences of gene regulatory network permutations for
the article ‘Local genetic context shapes the function of a gene regulatory network’,
Institute of Science and Technology Austria, 10.15479/AT:ISTA:8951.
mla: Nagy-Staron, Anna A. Sequences of Gene Regulatory Network Permutations for
the Article “Local Genetic Context Shapes the Function of a Gene Regulatory Network.”
Institute of Science and Technology Austria, 2020, doi:10.15479/AT:ISTA:8951.
short: A.A. Nagy-Staron, (2020).
contributor:
- contributor_type: project_member
first_name: Anna A
id: 3ABC5BA6-F248-11E8-B48F-1D18A9856A87
last_name: Nagy-Staron
- contributor_type: project_member
first_name: Kathrin
id: 3AEC8556-F248-11E8-B48F-1D18A9856A87
last_name: Tomasek
- contributor_type: project_member
first_name: Caroline
last_name: Caruso Carter
- contributor_type: project_member
first_name: Elisabeth
last_name: Sonnleitner
- contributor_type: project_member
first_name: Bor
id: 350F91D2-F248-11E8-B48F-1D18A9856A87
last_name: Kavcic
orcid: 0000-0001-6041-254X
- contributor_type: project_member
first_name: Tiago
last_name: Paixão
- contributor_type: project_manager
first_name: Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
date_created: 2020-12-20T10:00:26Z
date_published: 2020-12-21T00:00:00Z
date_updated: 2024-02-21T12:41:57Z
day: '21'
ddc:
- '570'
department:
- _id: CaGu
doi: 10.15479/AT:ISTA:8951
file:
- access_level: open_access
checksum: f57862aeee1690c7effd2b1117d40ed1
content_type: text/plain
creator: bkavcic
date_created: 2020-12-20T09:52:52Z
date_updated: 2020-12-20T09:52:52Z
file_id: '8952'
file_name: readme.txt
file_size: 523
relation: main_file
success: 1
- access_level: open_access
checksum: f2c6d5232ec6d551b6993991e8689e9f
content_type: application/octet-stream
creator: bkavcic
date_created: 2020-12-20T22:01:44Z
date_updated: 2020-12-20T22:01:44Z
file_id: '8954'
file_name: GRNs Research depository.gb
file_size: 379228
relation: main_file
success: 1
file_date_updated: 2020-12-20T22:01:44Z
has_accepted_license: '1'
keyword:
- Gene regulatory networks
- Gene expression
- Escherichia coli
- Synthetic Biology
month: '12'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '9283'
relation: used_in_publication
status: public
status: public
title: Sequences of gene regulatory network permutations for the article "Local genetic
context shapes the function of a gene regulatory network"
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '7383'
abstract:
- lang: eng
text: Organisms cope with change by employing transcriptional regulators. However,
when faced with rare environments, the evolution of transcriptional regulators
and their promoters may be too slow. We ask whether the intrinsic instability
of gene duplication and amplification provides a generic alternative to canonical
gene regulation. By real-time monitoring of gene copy number mutations in E. coli,
we show that gene duplications and amplifications enable adaptation to fluctuating
environments by rapidly generating copy number, and hence expression level, polymorphism.
This ‘amplification-mediated gene expression tuning’ occurs on timescales similar
to canonical gene regulation and can deal with rapid environmental changes. Mathematical
modeling shows that amplifications also tune gene expression in stochastic environments
where transcription factor-based schemes are hard to evolve or maintain. The fleeting
nature of gene amplifications gives rise to a generic population-level mechanism
that relies on genetic heterogeneity to rapidly tune expression of any gene, without
leaving any genomic signature.
article_processing_charge: No
author:
- first_name: Rok
full_name: Grah, Rok
id: 483E70DE-F248-11E8-B48F-1D18A9856A87
last_name: Grah
orcid: 0000-0003-2539-3560
citation:
ama: 'Grah R. Matlab scripts for the Paper: Gene Amplification as a Form of Population-Level
Gene Expression regulation. 2020. doi:10.15479/AT:ISTA:7383'
apa: 'Grah, R. (2020). Matlab scripts for the Paper: Gene Amplification as a Form
of Population-Level Gene Expression regulation. Institute of Science and Technology
Austria. https://doi.org/10.15479/AT:ISTA:7383'
chicago: 'Grah, Rok. “Matlab Scripts for the Paper: Gene Amplification as a Form
of Population-Level Gene Expression Regulation.” Institute of Science and Technology
Austria, 2020. https://doi.org/10.15479/AT:ISTA:7383.'
ieee: 'R. Grah, “Matlab scripts for the Paper: Gene Amplification as a Form of Population-Level
Gene Expression regulation.” Institute of Science and Technology Austria, 2020.'
ista: 'Grah R. 2020. Matlab scripts for the Paper: Gene Amplification as a Form
of Population-Level Gene Expression regulation, Institute of Science and Technology
Austria, 10.15479/AT:ISTA:7383.'
mla: 'Grah, Rok. Matlab Scripts for the Paper: Gene Amplification as a Form of
Population-Level Gene Expression Regulation. Institute of Science and Technology
Austria, 2020, doi:10.15479/AT:ISTA:7383.'
short: R. Grah, (2020).
contributor:
- contributor_type: project_leader
first_name: Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
date_created: 2020-01-28T10:41:49Z
date_published: 2020-01-28T00:00:00Z
date_updated: 2024-02-21T12:42:31Z
day: '28'
department:
- _id: CaGu
- _id: GaTk
doi: 10.15479/AT:ISTA:7383
file:
- access_level: open_access
checksum: 9d292cf5207b3829225f44c044cdb3fd
content_type: application/zip
creator: rgrah
date_created: 2020-01-28T10:39:40Z
date_updated: 2020-07-14T12:47:57Z
file_id: '7384'
file_name: Scripts.zip
file_size: 73363365
relation: main_file
- access_level: open_access
checksum: 4076ceab32ef588cc233802bab24c1ab
content_type: text/plain
creator: rgrah
date_created: 2020-01-28T10:39:30Z
date_updated: 2020-07-14T12:47:57Z
file_id: '7385'
file_name: READ_ME_MAIN.txt
file_size: 962
relation: main_file
file_date_updated: 2020-07-14T12:47:57Z
has_accepted_license: '1'
keyword:
- Matlab scripts
- analysis of microfluidics
- mathematical model
month: '01'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '7652'
relation: used_in_publication
status: public
status: public
title: 'Matlab scripts for the Paper: Gene Amplification as a Form of Population-Level
Gene Expression regulation'
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '9222'
article_processing_charge: No
author:
- first_name: Georgios
full_name: Katsaros, Georgios
id: 38DB5788-F248-11E8-B48F-1D18A9856A87
last_name: Katsaros
orcid: 0000-0001-8342-202X
citation:
ama: 'Katsaros G. Transport data for: Site‐controlled uniform Ge/Si Hut wires with
electrically tunable spin–orbit coupling. 2020. doi:10.15479/AT:ISTA:9222'
apa: 'Katsaros, G. (2020). Transport data for: Site‐controlled uniform Ge/Si Hut
wires with electrically tunable spin–orbit coupling. Institute of Science and
Technology Austria. https://doi.org/10.15479/AT:ISTA:9222'
chicago: 'Katsaros, Georgios. “Transport Data for: Site‐controlled Uniform Ge/Si
Hut Wires with Electrically Tunable Spin–Orbit Coupling.” Institute of Science
and Technology Austria, 2020. https://doi.org/10.15479/AT:ISTA:9222.'
ieee: 'G. Katsaros, “Transport data for: Site‐controlled uniform Ge/Si Hut wires
with electrically tunable spin–orbit coupling.” Institute of Science and Technology
Austria, 2020.'
ista: 'Katsaros G. 2020. Transport data for: Site‐controlled uniform Ge/Si Hut wires
with electrically tunable spin–orbit coupling, Institute of Science and Technology
Austria, 10.15479/AT:ISTA:9222.'
mla: 'Katsaros, Georgios. Transport Data for: Site‐controlled Uniform Ge/Si Hut
Wires with Electrically Tunable Spin–Orbit Coupling. Institute of Science
and Technology Austria, 2020, doi:10.15479/AT:ISTA:9222.'
short: G. Katsaros, (2020).
contributor:
- contributor_type: research_group
first_name: Georgios
id: 38DB5788-F248-11E8-B48F-1D18A9856A87
last_name: Katsaros
date_created: 2021-03-05T18:00:47Z
date_published: 2020-03-16T00:00:00Z
date_updated: 2024-02-21T12:42:13Z
day: '16'
ddc:
- '530'
department:
- _id: GeKa
doi: 10.15479/AT:ISTA:9222
file:
- access_level: open_access
checksum: 41b66e195ed3dbd73077feee77b05652
content_type: application/x-zip-compressed
creator: gkatsaro
date_created: 2021-03-05T17:50:45Z
date_updated: 2021-03-05T17:50:45Z
file_id: '9223'
file_name: DOI_SiteControlledHWs.zip
file_size: 13317557
relation: main_file
- access_level: open_access
checksum: a1dc5f710ba4b3bb7f248195ba754ab2
content_type: text/plain
creator: dernst
date_created: 2021-03-10T07:31:50Z
date_updated: 2021-03-10T07:31:50Z
file_id: '9233'
file_name: Readme.txt
file_size: 3515
relation: main_file
success: 1
file_date_updated: 2021-03-10T07:31:50Z
has_accepted_license: '1'
month: '03'
oa: 1
oa_version: Published Version
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '7541'
relation: used_in_publication
status: public
status: public
title: 'Transport data for: Site‐controlled uniform Ge/Si Hut wires with electrically
tunable spin–orbit coupling'
tmp:
image: /images/cc_0.png
legal_code_url: https://creativecommons.org/publicdomain/zero/1.0/legalcode
name: Creative Commons Public Domain Dedication (CC0 1.0)
short: CC0 (1.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '8375'
abstract:
- lang: eng
text: 'Supplementary movies showing the following sequences for spatio-temporarily
programmed shells: input geometry and actuation time landscape; comparison of
morphing processes from a camera recording and a simulation; final actuated shape.'
article_processing_charge: No
author:
- first_name: Ruslan
full_name: Guseinov, Ruslan
id: 3AB45EE2-F248-11E8-B48F-1D18A9856A87
last_name: Guseinov
orcid: 0000-0001-9819-5077
citation:
ama: 'Guseinov R. Supplementary data for “Computational design of curved thin shells:
from glass façades to programmable matter.” 2020. doi:10.15479/AT:ISTA:8375'
apa: 'Guseinov, R. (2020). Supplementary data for “Computational design of curved
thin shells: from glass façades to programmable matter.” Institute of Science
and Technology Austria. https://doi.org/10.15479/AT:ISTA:8375'
chicago: 'Guseinov, Ruslan. “Supplementary Data for ‘Computational Design of Curved
Thin Shells: From Glass Façades to Programmable Matter.’” Institute of Science
and Technology Austria, 2020. https://doi.org/10.15479/AT:ISTA:8375.'
ieee: 'R. Guseinov, “Supplementary data for ‘Computational design of curved thin
shells: from glass façades to programmable matter.’” Institute of Science and
Technology Austria, 2020.'
ista: 'Guseinov R. 2020. Supplementary data for ‘Computational design of curved
thin shells: from glass façades to programmable matter’, Institute of Science
and Technology Austria, 10.15479/AT:ISTA:8375.'
mla: 'Guseinov, Ruslan. Supplementary Data for “Computational Design of Curved
Thin Shells: From Glass Façades to Programmable Matter.” Institute of Science
and Technology Austria, 2020, doi:10.15479/AT:ISTA:8375.'
short: R. Guseinov, (2020).
contributor:
- contributor_type: researcher
first_name: Ruslan
id: 3AB45EE2-F248-11E8-B48F-1D18A9856A87
last_name: Guseinov
orcid: 0000-0001-9819-5077
- contributor_type: researcher
first_name: Connor
last_name: McMahan
- contributor_type: researcher
first_name: Jesus
id: 2DC83906-F248-11E8-B48F-1D18A9856A87
last_name: Perez Rodriguez
- contributor_type: researcher
first_name: Chiara
last_name: Daraio
- contributor_type: researcher
first_name: Bernd
id: 49876194-F248-11E8-B48F-1D18A9856A87
last_name: Bickel
orcid: 0000-0001-6511-9385
date_created: 2020-09-11T09:52:54Z
date_published: 2020-09-21T00:00:00Z
date_updated: 2024-02-21T12:44:29Z
day: '21'
ddc:
- '000'
department:
- _id: BeBi
doi: 10.15479/AT:ISTA:8375
ec_funded: 1
file:
- access_level: open_access
checksum: 4029ffd65fb82ef2366b2fc2a4908e16
content_type: video/mp4
creator: rguseino
date_created: 2020-09-11T09:45:21Z
date_updated: 2020-09-11T09:45:21Z
file_id: '8376'
file_name: supplementary_movie_1.mp4
file_size: 29214988
relation: main_file
success: 1
- access_level: open_access
checksum: 8ed03b04d80f1a4e622cb22e6100afd8
content_type: video/mp4
creator: rguseino
date_created: 2020-09-11T09:45:25Z
date_updated: 2020-09-11T09:45:25Z
file_id: '8377'
file_name: supplementary_movie_2.mp4
file_size: 28449475
relation: main_file
success: 1
- access_level: open_access
checksum: ad6864afb5e694e5c52a88fba4e02eea
content_type: video/mp4
creator: rguseino
date_created: 2020-09-11T09:45:28Z
date_updated: 2020-09-11T09:45:28Z
file_id: '8378'
file_name: supplementary_movie_3.mp4
file_size: 26315853
relation: main_file
success: 1
- access_level: open_access
checksum: b079cef7871fe1afb69af0e2b099f3b1
content_type: video/mp4
creator: rguseino
date_created: 2020-09-11T09:45:33Z
date_updated: 2020-09-11T09:45:33Z
file_id: '8379'
file_name: supplementary_movie_4.mp4
file_size: 25198755
relation: main_file
success: 1
- access_level: open_access
checksum: 9d1d48a8ed5c109a999c51b044ee523d
content_type: video/mp4
creator: rguseino
date_created: 2020-09-11T09:45:36Z
date_updated: 2020-09-11T09:45:36Z
file_id: '8380'
file_name: supplementary_movie_5.mp4
file_size: 29011354
relation: main_file
success: 1
- access_level: open_access
checksum: d414d0059e982d752d218756b3c3ce05
content_type: text/plain
creator: rguseino
date_created: 2020-09-11T09:52:36Z
date_updated: 2020-09-11T09:52:36Z
file_id: '8381'
file_name: readme.txt
file_size: 586
relation: main_file
success: 1
file_date_updated: 2020-09-11T09:52:36Z
has_accepted_license: '1'
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 24F9549A-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715767'
name: 'MATERIALIZABLE: Intelligent fabrication-oriented Computational Design and
Modeling'
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '8366'
relation: used_in_publication
status: public
status: public
title: 'Supplementary data for "Computational design of curved thin shells: from glass
façades to programmable matter"'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...