[{"publication":"iScience","citation":{"chicago":"Maes, Margaret E, Gloria Colombo, Florianne E Schoot Uiterkamp, Felix Sternberg, Alessandro Venturino, Elena E. Pohl, and Sandra Siegert. “Mitochondrial Network Adaptations of Microglia Reveal Sex-Specific Stress Response after Injury and UCP2 Knockout.” IScience. Elsevier, 2023. https://doi.org/10.1016/j.isci.2023.107780.","mla":"Maes, Margaret E., et al. “Mitochondrial Network Adaptations of Microglia Reveal Sex-Specific Stress Response after Injury and UCP2 Knockout.” IScience, vol. 26, no. 10, 107780, Elsevier, 2023, doi:10.1016/j.isci.2023.107780.","short":"M.E. Maes, G. Colombo, F.E. Schoot Uiterkamp, F. Sternberg, A. Venturino, E.E. Pohl, S. Siegert, IScience 26 (2023).","ista":"Maes ME, Colombo G, Schoot Uiterkamp FE, Sternberg F, Venturino A, Pohl EE, Siegert S. 2023. Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout. iScience. 26(10), 107780.","ieee":"M. E. Maes et al., “Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout,” iScience, vol. 26, no. 10. Elsevier, 2023.","apa":"Maes, M. E., Colombo, G., Schoot Uiterkamp, F. E., Sternberg, F., Venturino, A., Pohl, E. E., & Siegert, S. (2023). Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout. IScience. Elsevier. https://doi.org/10.1016/j.isci.2023.107780","ama":"Maes ME, Colombo G, Schoot Uiterkamp FE, et al. Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout. iScience. 2023;26(10). doi:10.1016/j.isci.2023.107780"},"article_type":"original","date_published":"2023-10-20T00:00:00Z","scopus_import":"1","day":"20","has_accepted_license":"1","article_processing_charge":"Yes","_id":"14363","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout","ddc":["570"],"status":"public","intvolume":" 26","oa_version":"Published Version","file":[{"file_id":"14497","relation":"main_file","date_created":"2023-11-07T08:53:21Z","date_updated":"2023-11-07T08:53:21Z","success":1,"checksum":"be1a560efdd96d20712311f4fc54aac2","file_name":"2023_iScience_Maes.pdf","access_level":"open_access","creator":"dernst","file_size":8197935,"content_type":"application/pdf"}],"type":"journal_article","abstract":[{"text":"Mitochondrial networks remodel their connectivity, content, and subcellular localization to support optimized energy production in conditions of increased environmental or cellular stress. Microglia rely on mitochondria to respond to these stressors, however our knowledge about mitochondrial networks and their adaptations in microglia in vivo is limited. Here, we generate a mouse model that selectively labels mitochondria in microglia. We identify that mitochondrial networks are more fragmented with increased content and perinuclear localization in vitro vs. in vivo. Mitochondrial networks adapt similarly in microglia closest to the injury site after optic nerve crush. Preventing microglial UCP2 increase after injury by selective knockout induces cellular stress. This results in mitochondrial hyperfusion in male microglia, a phenotype absent in females due to circulating estrogens. Our results establish the foundation for mitochondrial network analysis of microglia in vivo, emphasizing the importance of mitochondrial-based sex effects of microglia in other pathologies.","lang":"eng"}],"issue":"10","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["001080403500001"],"pmid":["37731609"]},"oa":1,"quality_controlled":"1","isi":1,"doi":"10.1016/j.isci.2023.107780","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"language":[{"iso":"eng"}],"month":"10","publication_identifier":{"eissn":["2589-0042"]},"acknowledgement":"We thank the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging and Optics Facility (IOF), the Lab Support Facility (LSF), and the Pre-Clinical Facility (PCF) team, specifically Sonja Haslinger and Michael Schunn for excellent mouse colony management and support. This research was supported by the FWF Sonderforschungsbereich F83 (to E.E.P). We thank Bálint Nagy, Ryan John A. Cubero, Marco Benevento and all members of the Siegert group for constant feedback on the project and article.","year":"2023","pmid":1,"publication_status":"published","department":[{"_id":"SaSi"}],"publisher":"Elsevier","author":[{"full_name":"Maes, Margaret E","first_name":"Margaret E","last_name":"Maes","id":"3838F452-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9642-1085"},{"full_name":"Colombo, Gloria","first_name":"Gloria","last_name":"Colombo","id":"3483CF6C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9434-8902"},{"full_name":"Schoot Uiterkamp, Florianne E","first_name":"Florianne E","last_name":"Schoot Uiterkamp","id":"3526230C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Sternberg, Felix","last_name":"Sternberg","first_name":"Felix"},{"full_name":"Venturino, Alessandro","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2356-9403","first_name":"Alessandro","last_name":"Venturino"},{"last_name":"Pohl","first_name":"Elena E.","full_name":"Pohl, Elena E."},{"orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","last_name":"Siegert","first_name":"Sandra","full_name":"Siegert, Sandra"}],"date_created":"2023-09-24T22:01:11Z","date_updated":"2023-12-13T12:27:30Z","volume":26,"article_number":"107780","file_date_updated":"2023-11-07T08:53:21Z"},{"article_type":"original","citation":{"chicago":"Maes, Margaret E, Ryan J. Donahue, Cassandra L. Schlamp, Olivia J. Marola, Richard T. Libby, and Robert W. Nickells. “BAX Activation in Mouse Retinal Ganglion Cells Occurs in Two Temporally and Mechanistically Distinct Steps.” Molecular Neurodegeneration. Springer Nature, 2023. https://doi.org/10.1186/s13024-023-00659-8.","mla":"Maes, Margaret E., et al. “BAX Activation in Mouse Retinal Ganglion Cells Occurs in Two Temporally and Mechanistically Distinct Steps.” Molecular Neurodegeneration, vol. 18, 67, Springer Nature, 2023, doi:10.1186/s13024-023-00659-8.","short":"M.E. Maes, R.J. Donahue, C.L. Schlamp, O.J. Marola, R.T. Libby, R.W. Nickells, Molecular Neurodegeneration 18 (2023).","ista":"Maes ME, Donahue RJ, Schlamp CL, Marola OJ, Libby RT, Nickells RW. 2023. BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps. Molecular Neurodegeneration. 18, 67.","apa":"Maes, M. E., Donahue, R. J., Schlamp, C. L., Marola, O. J., Libby, R. T., & Nickells, R. W. (2023). BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps. Molecular Neurodegeneration. Springer Nature. https://doi.org/10.1186/s13024-023-00659-8","ieee":"M. E. Maes, R. J. Donahue, C. L. Schlamp, O. J. Marola, R. T. Libby, and R. W. Nickells, “BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps,” Molecular Neurodegeneration, vol. 18. Springer Nature, 2023.","ama":"Maes ME, Donahue RJ, Schlamp CL, Marola OJ, Libby RT, Nickells RW. BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps. Molecular Neurodegeneration. 2023;18. doi:10.1186/s13024-023-00659-8"},"publication":"Molecular Neurodegeneration","date_published":"2023-09-26T00:00:00Z","scopus_import":"1","article_processing_charge":"Yes","has_accepted_license":"1","day":"26","intvolume":" 18","status":"public","ddc":["570"],"title":"BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"14401","oa_version":"Published Version","file":[{"relation":"main_file","file_id":"14917","date_updated":"2024-01-30T14:33:31Z","date_created":"2024-01-30T14:33:31Z","checksum":"3aa218ddea4a082d8fd5e196ae55ca06","success":1,"file_name":"2023_MolecularNeurodegeneration_Maes.pdf","access_level":"open_access","content_type":"application/pdf","file_size":11568350,"creator":"dernst"}],"type":"journal_article","abstract":[{"lang":"eng","text":"Background: \r\nPro-apoptotic BAX is a central mediator of retinal ganglion cell (RGC) death after optic nerve damage. BAX activation occurs in two stages including translocation of latent BAX to the mitochondrial outer membrane (MOM) and then permeabilization of the MOM to facilitate the release of apoptotic signaling molecules. As a critical component of RGC death, BAX is an attractive target for neuroprotective therapies and an understanding of the kinetics of BAX activation and the mechanisms controlling the two stages of this process in RGCs is potentially valuable in informing the development of a neuroprotective strategy.\r\nMethods:\r\nThe kinetics of BAX translocation were assessed by both static and live-cell imaging of a GFP-BAX fusion protein introduced into RGCs using AAV2-mediated gene transfer in mice. Activation of BAX was achieved using an acute optic nerve crush (ONC) protocol. Live-cell imaging of GFP-BAX was achieved using explants of mouse retina harvested 7 days after ONC. Kinetics of translocation in RGCs were compared to GFP-BAX translocation in 661W tissue culture cells. Permeabilization of GFP-BAX was assessed by staining with the 6A7 monoclonal antibody, which recognizes a conformational change in this protein after MOM insertion. Assessment of individual kinases associated with both stages of activation was made using small molecule inhibitors injected into the vitreous either independently or in concert with ONC surgery. The contribution of the Dual Leucine Zipper-JUN-N-Terminal Kinase cascade was evaluated using mice with a double conditional knock-out of both Mkk4 and Mkk7.\r\nResults:\r\nONC induces the translocation of GFP-BAX in RGCs at a slower rate and with less intracellular synchronicity than 661W cells, but exhibits less variability among mitochondrial foci within a single cell. GFP-BAX was also found to translocate in all compartments of an RGC including the dendritic arbor and axon. Approximately 6% of translocating RGCs exhibited retrotranslocation of BAX immediately following translocation. Unlike tissue culture cells, which exhibit simultaneous translocation and permeabilization, RGCs exhibited a significant delay between these two stages, similar to detached cells undergoing anoikis. Translocation, with minimal permeabilization could be induced in a subset of RGCs using an inhibitor of Focal Adhesion Kinase (PF573228). Permeabilization after ONC, in a majority of RGCs, could be inhibited with a broad spectrum kinase inhibitor (sunitinib) or a selective inhibitor for p38/MAPK14 (SB203580). Intervention of DLK-JNK axis signaling abrogated GFP-BAX translocation after ONC.\r\nConclusions:\r\nA comparison between BAX activation kinetics in tissue culture cells and in cells of a complex tissue environment shows distinct differences indicating that caution should be used when translating findings from one condition to the other. RGCs exhibit both a delay between translocation and permeabilization and the ability for translocated BAX to be retrotranslocated, suggesting several stages at which intervention of the activation process could be exploited in the design of a therapeutic strategy."}],"isi":1,"quality_controlled":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"pmid":["37292963"],"isi":["001071403800001"]},"language":[{"iso":"eng"}],"doi":"10.1186/s13024-023-00659-8","publication_identifier":{"eissn":["1750-1326"]},"month":"09","publisher":"Springer Nature","department":[{"_id":"SaSi"}],"publication_status":"published","pmid":1,"acknowledgement":"The authors would like to thank Mr. Joel Dietz for management of the mouse colony and helpful advice for conducting quantitative PCR studies and Mr. Santoshi Kinoshita at the Translational Research Initiative in Pathology laboratory at the University of Wisconsin-Madison for cutting sections analyzed in this study.\r\nThis work was supported by National Eye Institute grants R01 EY030123 (RWN), R01 EY018606 (RTL), P30 EY016665 (Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison), T32 EY027721 (RJD) and F31 EY030739 (OJM). Additional funding was provided by the BrightFocus Foundation (RWN) and unrestricted grants from Research to Prevent Blindness, Inc to the Department of Ophthalmology and Visual Sciences (University of Wisconsin-Madison) and to the Department of Ophthalmology (University of Rochester).","year":"2023","volume":18,"date_created":"2023-10-08T22:01:15Z","date_updated":"2024-01-30T14:34:21Z","author":[{"orcid":"0000-0001-9642-1085","id":"3838F452-F248-11E8-B48F-1D18A9856A87","last_name":"Maes","first_name":"Margaret E","full_name":"Maes, Margaret E"},{"first_name":"Ryan J.","last_name":"Donahue","full_name":"Donahue, Ryan J."},{"full_name":"Schlamp, Cassandra L.","first_name":"Cassandra L.","last_name":"Schlamp"},{"first_name":"Olivia J.","last_name":"Marola","full_name":"Marola, Olivia J."},{"last_name":"Libby","first_name":"Richard T.","full_name":"Libby, Richard T."},{"full_name":"Nickells, Robert W.","first_name":"Robert W.","last_name":"Nickells"}],"article_number":"67","file_date_updated":"2024-01-30T14:33:31Z"},{"month":"08","project":[{"grant_number":"I03600","_id":"265CB4D0-B435-11E9-9278-68D0E5697425","name":"Optical control of synaptic function via adhesion molecules","call_identifier":"FWF"},{"_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24","name":"Molecular Drug Targets","call_identifier":"FWF"},{"name":"The Wittgenstein Prize","call_identifier":"FWF","grant_number":"Z00312","_id":"25C5A090-B435-11E9-9278-68D0E5697425"},{"_id":"23889792-32DE-11EA-91FC-C7463DDC885E","name":"High content imaging to decode human immune cell interactions in health and allergic disease"},{"_id":"25444568-B435-11E9-9278-68D0E5697425","grant_number":"715508","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","call_identifier":"H2020"},{"_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glumatergic synapse","call_identifier":"H2020"},{"call_identifier":"H2020","name":"International IST Doctoral Program","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","grant_number":"101026635","call_identifier":"H2020","name":"Synaptic computations of the hippocampal CA3 circuitry"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","image":"/images/cc_by_nc_sa.png","short":"CC BY-NC-SA (4.0)"},"oa":1,"acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"doi":"10.15479/AT:ISTA:13126","license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","ec_funded":1,"file_date_updated":"2023-08-04T13:19:47Z","publisher":"Institute of Science and Technology Austria","department":[{"_id":"JoDa"},{"_id":"SaSi"},{"_id":"GaNo"},{"_id":"PeJo"},{"_id":"Bio"},{"_id":"RySh"}],"acknowledgement":"We thank Jakob Vorlaufer, Nathalie Agudelo-Dueñas, Wiebke Jahr, Andreas Wartak for microscope maintenance and troubleshooting, Caroline Kreuzinger, Anna Freeman, and Irene Erber for technical assistance and Matthias Tomschik for support with obtaining human samples. We gratefully acknowledge Eder Miguel for setting up webKnossos and Marek Šuplata for computational support and hardware control. We are grateful to Ryuichi Shigemoto and Bernd Bickel for generous support, and Michael Sixt and Scott Boyd (Stanford University) for discussions and critical reading of the manuscript. PSD95-HaloTag mice were kindly provided by Seth Grant (University of Edinburgh). We acknowledge expert support by IST Austria’s scientific computing, imaging and optics, preclinical, and lab support facilities, and by the Library and Miba machine shop.\r\nWe gratefully acknowledge funding by the following sources: \r\nAustrian Science Fund (FWF) grant I3600-B27 (JGD)\r\nAustrian Science Fund (FWF) grant DK W1232 (JGD, JMM)\r\nAustrian Science Fund (FWF) grant Z 312-B27, Wittgenstein award (PJ)\r\nAustrian Science Funds (FWF) projects I4685-B, I6565-B (SYNABS) and DOC 33-B27 (RH)\r\nGesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (JGD)\r\nEuropean Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 715508 – REVERSEAUTISM (GN)\r\nEuropean Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 692692 – GIANTSYN (PJ)\r\nMarie Skłodowska-Curie Actions Fellowship GA no. 665385 under the EU Horizon 2020 program (JMM, JL)\r\nMarie Skłodowska-Curie Actions Individual Fellowship 101026635 under the EU Horizon 2020 program (JFW)","year":"2023","date_updated":"2024-02-21T12:18:19Z","date_created":"2023-06-07T07:15:12Z","related_material":{"link":[{"url":"https://pub.ista.ac.at/group_danzl/data/CATS/","description":"Original data for Fig. 5d, Fig. 5d (N2V) and Fig. 5f-i, provided via an external link due to the large size (>10GB) of the datasets. ","relation":"research_data"}],"record":[{"status":"public","relation":"used_in_publication","id":"14257"}]},"contributor":[{"id":"443DB6DE-F248-11E8-B48F-1D18A9856A87","last_name":"Michalska","first_name":"Julia M"},{"first_name":"Julia","last_name":"Lyudchik","id":"46E28B80-F248-11E8-B48F-1D18A9856A87"},{"id":"39BDC62C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2340-7431","first_name":"Philipp","last_name":"Velicky"},{"id":"ee3cb6ca-ec98-11ea-ae11-ff703e2254ed","first_name":"Hana","last_name":"Stefanickova"},{"orcid":"0000-0002-8698-3823","id":"63836096-4690-11EA-BD4E-32803DDC885E","last_name":"Watson","first_name":"Jake"},{"first_name":"Alban","last_name":"Cenameri","id":"9ac8f577-2357-11eb-997a-e566c5550886"},{"id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","first_name":"Christoph M","last_name":"Sommer"},{"orcid":"0000-0002-3183-8207","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","last_name":"Amberg","first_name":"Nicole"},{"id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2356-9403","first_name":"Alessandro","last_name":"Venturino"},{"last_name":"Roessler","first_name":"Karl"},{"first_name":"Thomas","last_name":"Czech"},{"first_name":"Romana","last_name":"Höftberger"},{"last_name":"Siegert","first_name":"Sandra","orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87"},{"id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178","first_name":"Gaia","last_name":"Novarino"},{"last_name":"Jonas","first_name":"Peter M","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"author":[{"full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","first_name":"Johann G"}],"article_processing_charge":"No","has_accepted_license":"1","day":"04","citation":{"chicago":"Danzl, Johann G. “Research Data for the Publication ‘Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.’” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/AT:ISTA:13126.","short":"J.G. Danzl, (2023).","mla":"Danzl, Johann G. Research Data for the Publication “Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.” Institute of Science and Technology Austria, 2023, doi:10.15479/AT:ISTA:13126.","ieee":"J. G. Danzl, “Research data for the publication ‘Imaging brain tissue architecture across millimeter to nanometer scales.’” Institute of Science and Technology Austria, 2023.","apa":"Danzl, J. G. (2023). Research data for the publication “Imaging brain tissue architecture across millimeter to nanometer scales.” Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:13126","ista":"Danzl JG. 2023. Research data for the publication ‘Imaging brain tissue architecture across millimeter to nanometer scales’, Institute of Science and Technology Austria, 10.15479/AT:ISTA:13126.","ama":"Danzl JG. Research data for the publication “Imaging brain tissue architecture across millimeter to nanometer scales.” 2023. doi:10.15479/AT:ISTA:13126"},"date_published":"2023-08-04T00:00:00Z","type":"research_data","abstract":[{"lang":"eng","text":"Mapping the complex and dense arrangement of cells and their connectivity in brain tissue demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels at visualizing specific molecules and individual cells but fails to provide tissue context. Here, we developed Comprehensive Analysis of Tissues across Scales (CATS), a technology to densely map brain tissue architecture from millimeter regional to nanometer synaptic scales in diverse chemically fixed brain preparations, including rodent and human. CATS uses fixation-compatible extracellular labeling and optical imaging, including stimulated emission depletion or expansion microscopy, to comprehensively delineate cellular structures. It enables three-dimensional reconstruction of single synapses and mapping of synaptic connectivity by identification and analysis of putative synaptic cleft regions. Applying CATS to the mouse hippocampal mossy fiber circuitry, we reconstructed and quantified the synaptic input and output structure of identified neurons. We furthermore demonstrate applicability to clinically derived human tissue samples, including formalin-fixed paraffin-embedded routine diagnostic specimens, for visualizing the cellular architecture of brain tissue in health and disease."}],"title":"Research data for the publication \"Imaging brain tissue architecture across millimeter to nanometer scales\"","ddc":["610"],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"13126","oa_version":"Published Version","file":[{"content_type":"text/plain","file_size":541,"creator":"jdanzl","access_level":"open_access","file_name":"Readme_Michalska_2023.txt","checksum":"6f18ce9b89b47ce5abeb379869ff5c49","success":1,"date_updated":"2023-08-04T13:19:47Z","date_created":"2023-08-04T13:19:47Z","relation":"main_file","file_id":"13961"},{"file_id":"13482","relation":"main_file","date_updated":"2023-08-03T11:29:29Z","date_created":"2023-08-03T11:29:29Z","success":1,"checksum":"2098e8c5285c5e86cb69075e1b5dcf39","file_name":"Fig1_b_top-left.tif","access_level":"open_access","creator":"jdanzl","file_size":64582744,"content_type":"image/tiff"},{"success":1,"checksum":"9affab3dacf847096cc8e8d5ff99467e","date_updated":"2023-08-03T11:29:28Z","date_created":"2023-08-03T11:29:28Z","file_id":"13483","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":1442113,"access_level":"open_access","file_name":"Fig1_b_top-right.tif"},{"relation":"main_file","file_id":"13484","checksum":"d7fec0296123a17f6bcdbfee17411424","success":1,"date_updated":"2023-08-03T11:29:29Z","date_created":"2023-08-03T11:29:29Z","access_level":"open_access","file_name":"Fig1_c_1.tif","content_type":"image/tiff","file_size":40078276,"creator":"jdanzl"},{"file_name":"Fig1_c_2.tif","access_level":"open_access","file_size":7984315,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13485","date_updated":"2023-08-03T11:29:29Z","date_created":"2023-08-03T11:29:29Z","checksum":"5195a180c4f26a7adc994bad0b3c53c7","success":1},{"checksum":"df1105d8f41ebd5373cacc597ffa91f3","success":1,"date_updated":"2023-08-03T11:29:29Z","date_created":"2023-08-03T11:29:29Z","relation":"main_file","file_id":"13486","file_size":8693320,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Fig1_c_3.tif"},{"file_size":6003063,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Fig1_c_4.tif","checksum":"00edbb0c9f42032c830d2291ec55c4c0","success":1,"date_updated":"2023-08-03T11:29:30Z","date_created":"2023-08-03T11:29:30Z","relation":"main_file","file_id":"13487"},{"success":1,"checksum":"a58399e98ec4b6506a5dae13e387f391","date_updated":"2023-08-03T11:29:30Z","date_created":"2023-08-03T11:29:30Z","file_id":"13488","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":24003063,"access_level":"open_access","file_name":"Fig1_d.tif"},{"creator":"jdanzl","content_type":"image/tiff","file_size":18063,"access_level":"open_access","file_name":"Fig1_e_zoom.tif","success":1,"checksum":"699df4bc104df14607359678963b3d2d","date_updated":"2023-08-03T11:29:30Z","date_created":"2023-08-03T11:29:30Z","file_id":"13489","relation":"main_file"},{"relation":"main_file","file_id":"13490","checksum":"ea7b1278da490d221fc7698fd6bc39a4","success":1,"date_created":"2023-08-03T11:29:30Z","date_updated":"2023-08-03T11:29:30Z","access_level":"open_access","file_name":"Fig1_e.tif","file_size":186813,"content_type":"image/tiff","creator":"jdanzl"},{"file_size":17494,"content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","creator":"jdanzl","file_name":"Fig1_f.xlsx","access_level":"open_access","date_created":"2023-08-03T11:29:30Z","date_updated":"2023-08-03T11:29:30Z","checksum":"37b05ef3a36b9f6bf4ec46199230874a","success":1,"relation":"main_file","file_id":"13491"},{"file_id":"13492","relation":"main_file","success":1,"checksum":"872eb3dc1a94afb26947a365bfae581a","date_created":"2023-08-03T11:40:43Z","date_updated":"2023-08-03T11:40:43Z","access_level":"open_access","file_name":"Fig2_a_1.tif","creator":"jdanzl","content_type":"image/tiff","file_size":816786862},{"file_name":"Fig2_a_2.tif","access_level":"open_access","file_size":8654692,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13493","date_updated":"2023-08-03T11:40:13Z","date_created":"2023-08-03T11:40:13Z","checksum":"cab6f72d7d546a81c105c229747949ef","success":1},{"date_updated":"2023-08-03T11:40:14Z","date_created":"2023-08-03T11:40:14Z","checksum":"c85bc3ffe7f6bdf16a81290761ed6253","success":1,"relation":"main_file","file_id":"13494","content_type":"image/tiff","file_size":8654692,"creator":"jdanzl","file_name":"Fig2_a_3.tif","access_level":"open_access"},{"file_id":"13495","relation":"main_file","date_created":"2023-08-03T11:40:24Z","date_updated":"2023-08-03T11:40:24Z","success":1,"checksum":"5a3fd5484e566c34e210a4acb2b97791","file_name":"Fig2_a_4.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":272262438},{"checksum":"ae5cbacf56e3fdf934a6d6fd489f5fe0","success":1,"date_updated":"2023-08-03T11:40:37Z","date_created":"2023-08-03T11:40:37Z","relation":"main_file","file_id":"13496","content_type":"image/tiff","file_size":272262438,"creator":"jdanzl","access_level":"open_access","file_name":"Fig2_a_5.tif"},{"content_type":"image/tiff","file_size":204301228,"creator":"jdanzl","file_name":"Fig2_a_6.tif","access_level":"open_access","date_created":"2023-08-03T11:40:44Z","date_updated":"2023-08-03T11:40:44Z","checksum":"e6da436b83bbe3a34138b52e5de49d69","success":1,"relation":"main_file","file_id":"13497"},{"date_updated":"2023-08-03T11:40:49Z","date_created":"2023-08-03T11:40:49Z","success":1,"checksum":"855f0a0bcf5b3d7cc7d50561f2c8f625","file_id":"13498","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":68094350,"file_name":"Fig2_a_7-8.tif","access_level":"open_access"},{"access_level":"open_access","file_name":"Fig2_a_roi.roi","creator":"jdanzl","file_size":156,"content_type":"application/octet-stream","file_id":"13499","relation":"main_file","success":1,"checksum":"ff709f9f8ebaa8cf50a4b2fca5ce9813","date_updated":"2023-08-03T11:40:48Z","date_created":"2023-08-03T11:40:48Z"},{"creator":"jdanzl","file_size":1036927887,"content_type":"image/tiff","file_name":"Fig2_c-i_1_n2v.tif","access_level":"open_access","date_created":"2023-08-03T11:41:37Z","date_updated":"2023-08-03T11:41:37Z","success":1,"checksum":"ce9b774ff5163ebfa4e3fb162f4cfffe","file_id":"13500","relation":"main_file"},{"file_name":"Fig2_c-i_1.tif","access_level":"open_access","creator":"jdanzl","file_size":518519275,"content_type":"image/tiff","file_id":"13501","relation":"main_file","date_updated":"2023-08-03T11:41:25Z","date_created":"2023-08-03T11:41:25Z","success":1,"checksum":"21f1bb8d2a00c88bdd6febe6285acc2a"},{"checksum":"9af5b66b74c2ff4b2b99b0d94f2864e6","success":1,"date_updated":"2023-08-03T11:42:48Z","date_created":"2023-08-03T11:42:48Z","relation":"main_file","file_id":"13502","file_size":1444202302,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Fig2_c-i_3_n2v.tif"},{"success":1,"checksum":"c83261e88821747b301197314d92f2b2","date_updated":"2023-08-03T11:42:57Z","date_created":"2023-08-03T11:42:57Z","file_id":"13503","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":1075338708,"access_level":"open_access","file_name":"Fig2_c-i_3.tif"},{"file_name":"Fig2_c-i_2_n2v.tif","access_level":"open_access","creator":"jdanzl","file_size":851145493,"content_type":"image/tiff","file_id":"13504","relation":"main_file","date_updated":"2023-08-03T11:44:05Z","date_created":"2023-08-03T11:44:05Z","success":1,"checksum":"5d1278ba4b7e085c0c186ea49fce535b"},{"relation":"main_file","file_id":"13505","checksum":"5aaf1eba65582cf437ff3ce753eba4d7","success":1,"date_updated":"2023-08-03T11:43:47Z","date_created":"2023-08-03T11:43:47Z","access_level":"open_access","file_name":"Fig2_c-i_2.tif","file_size":432099834,"content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","file_size":1036927887,"file_name":"Fig2_h_n2v.tif","access_level":"open_access","date_created":"2023-08-03T11:44:41Z","date_updated":"2023-08-03T11:44:41Z","success":1,"checksum":"ce9b774ff5163ebfa4e3fb162f4cfffe","file_id":"13506","relation":"main_file"},{"creator":"jdanzl","content_type":"image/tiff","file_size":518519275,"access_level":"open_access","file_name":"Fig2_h.tif","success":1,"checksum":"21f1bb8d2a00c88bdd6febe6285acc2a","date_updated":"2023-08-03T11:44:36Z","date_created":"2023-08-03T11:44:36Z","file_id":"13507","relation":"main_file"},{"content_type":"image/tiff","file_size":19287397,"creator":"jdanzl","file_name":"Fig2_j_1.tif","access_level":"open_access","date_updated":"2023-08-03T11:44:40Z","date_created":"2023-08-03T11:44:40Z","checksum":"b37f25a90ef42fa4358649fdd6b41f35","success":1,"relation":"main_file","file_id":"13508"},{"relation":"main_file","file_id":"13509","date_updated":"2023-08-03T11:44:41Z","date_created":"2023-08-03T11:44:41Z","checksum":"50f0969c6c2c1209ff776bec4861030a","success":1,"file_name":"Fig2_j_2.tif","access_level":"open_access","content_type":"image/tiff","file_size":617552,"creator":"jdanzl"},{"content_type":"image/tiff","file_size":19287392,"creator":"jdanzl","file_name":"Fig2_j_3.tif","access_level":"open_access","date_updated":"2023-08-03T11:44:41Z","date_created":"2023-08-03T11:44:41Z","checksum":"2a8cc93471378ac4c3b640e4318d89eb","success":1,"relation":"main_file","file_id":"13510"},{"access_level":"open_access","file_name":"Fig2_j_4.tif","content_type":"image/tiff","file_size":617552,"creator":"jdanzl","relation":"main_file","file_id":"13511","checksum":"b90965d20133fea73c99050c32eb7695","success":1,"date_created":"2023-08-03T11:44:42Z","date_updated":"2023-08-03T11:44:42Z"},{"file_name":"Fig2_j_coCATS_N2V.tif","access_level":"open_access","creator":"jdanzl","file_size":19287339,"content_type":"image/tiff","file_id":"13512","relation":"main_file","date_updated":"2023-08-03T11:44:43Z","date_created":"2023-08-03T11:44:43Z","success":1,"checksum":"568a1e0efaa62527b51e66a7aabfd05c"},{"relation":"main_file","file_id":"13513","date_created":"2023-08-03T11:44:44Z","date_updated":"2023-08-03T11:44:44Z","checksum":"c70447cebe073dd78e0ac34ecc36ad85","success":1,"file_name":"Fig2_j_roi.roi","access_level":"open_access","file_size":148,"content_type":"application/octet-stream","creator":"jdanzl"},{"access_level":"open_access","file_name":"Fig3_a_n2v.tif","creator":"jdanzl","file_size":316842690,"content_type":"image/tiff","file_id":"13514","relation":"main_file","success":1,"checksum":"505252cc4b443b76c2e41ecf5879a542","date_updated":"2023-08-03T11:44:52Z","date_created":"2023-08-03T11:44:52Z"},{"date_updated":"2023-08-03T11:45:01Z","date_created":"2023-08-03T11:45:01Z","checksum":"35463183611e8ee4fba2025772c40eb2","success":1,"relation":"main_file","file_id":"13515","file_size":172839057,"content_type":"image/tiff","creator":"jdanzl","file_name":"Fig3_a.tif","access_level":"open_access"},{"access_level":"open_access","file_name":"Fig3_g_n2v.tif","creator":"jdanzl","content_type":"image/tiff","file_size":309407894,"file_id":"13516","relation":"main_file","success":1,"checksum":"e0469102a87332b46904b4ba35b8fb2f","date_created":"2023-08-03T11:45:05Z","date_updated":"2023-08-03T11:45:05Z"},{"file_size":11327792,"content_type":"image/tiff","creator":"jdanzl","file_name":"Fig4_a_MIP.tif","access_level":"open_access","date_updated":"2023-08-03T11:51:15Z","date_created":"2023-08-03T11:51:15Z","checksum":"854c8fe02acd920f8e8f6a3173458e86","success":1,"relation":"main_file","file_id":"13517"},{"creator":"jdanzl","file_size":106696700,"content_type":"image/tiff","file_name":"Fig4_c_dghilus_bouton2_n2v.tif","access_level":"open_access","date_created":"2023-08-03T11:51:18Z","date_updated":"2023-08-03T11:51:18Z","success":1,"checksum":"ab35811ffbe3f40733d0e054d9d6c4e6","file_id":"13518","relation":"main_file"},{"creator":"jdanzl","content_type":"image/tiff","file_size":53367287,"file_name":"Fig4_c_dghilus_bouton2.tif","access_level":"open_access","date_updated":"2023-08-03T11:51:17Z","date_created":"2023-08-03T11:51:17Z","success":1,"checksum":"e3b6410d68e42004ef3857967707aeb3","file_id":"13519","relation":"main_file"},{"relation":"main_file","file_id":"13520","checksum":"9ef0e3f3ec6f0b7b2666e3a0d7e6f355","success":1,"date_created":"2023-08-03T11:51:20Z","date_updated":"2023-08-03T11:51:20Z","access_level":"open_access","file_name":"Fig4_c_dghilus_bouton5_n2v.tif","file_size":103816699,"content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","file_size":51927287,"access_level":"open_access","file_name":"Fig4_c_dghilus_bouton5.tif","success":1,"checksum":"d5d846a4edaf0168ca6cc53d8cc39dde","date_created":"2023-08-03T11:51:21Z","date_updated":"2023-08-03T11:51:21Z","file_id":"13521","relation":"main_file"},{"file_size":76391971,"content_type":"image/tiff","creator":"jdanzl","file_name":"Fig4_c_stratum_bouton11_n2v.tif","access_level":"open_access","date_updated":"2023-08-03T11:51:24Z","date_created":"2023-08-03T11:51:24Z","checksum":"690a87182441c9742da2550189641e5a","success":1,"relation":"main_file","file_id":"13522"},{"file_name":"Fig4_c_stratum_bouton11.tif","access_level":"open_access","file_size":38217920,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13523","date_updated":"2023-08-03T11:51:23Z","date_created":"2023-08-03T11:51:23Z","checksum":"4dc575424729c5ba181bd9a3962d4781","success":1},{"relation":"main_file","file_id":"13524","date_created":"2023-08-03T11:51:29Z","date_updated":"2023-08-03T11:51:29Z","checksum":"9fdd51636928f45e8e534cc5a37a48ff","success":1,"file_name":"Fig4_c_stratum_bouton13_n2v.tif","access_level":"open_access","file_size":154149020,"content_type":"image/tiff","creator":"jdanzl"},{"file_id":"13525","relation":"main_file","date_updated":"2023-08-03T11:51:28Z","date_created":"2023-08-03T11:51:28Z","success":1,"checksum":"643169464252d67753ac6f8493668f34","file_name":"Fig4_c_stratum_bouton13.tif","access_level":"open_access","creator":"jdanzl","file_size":77099447,"content_type":"image/tiff"},{"checksum":"ab35811ffbe3f40733d0e054d9d6c4e6","success":1,"date_created":"2023-08-03T11:51:32Z","date_updated":"2023-08-03T11:51:32Z","relation":"main_file","file_id":"13526","content_type":"image/tiff","file_size":106696700,"creator":"jdanzl","access_level":"open_access","file_name":"Fig4_d.tif"},{"success":1,"checksum":"9fdd51636928f45e8e534cc5a37a48ff","date_updated":"2023-08-03T11:51:35Z","date_created":"2023-08-03T11:51:35Z","file_id":"13527","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":154149020,"access_level":"open_access","file_name":"Fig4_e.tif"},{"content_type":"image/tiff","file_size":5986760,"creator":"jdanzl","access_level":"open_access","file_name":"Fig4_f_1_top.tif","checksum":"5380d0a98e97d7d8462b38bd0a96644c","success":1,"date_updated":"2023-08-03T11:51:32Z","date_created":"2023-08-03T11:51:32Z","relation":"main_file","file_id":"13528"},{"file_id":"13529","relation":"main_file","success":1,"checksum":"7d1309e806eb31588ebafcf40388e13f","date_updated":"2023-08-03T11:51:33Z","date_created":"2023-08-03T11:51:33Z","access_level":"open_access","file_name":"Fig4_f_2_top.tif","creator":"jdanzl","file_size":6001178,"content_type":"image/tiff"},{"success":1,"checksum":"a71c80207655d508cbc7626bc144d7a1","date_created":"2023-08-03T11:51:33Z","date_updated":"2023-08-03T11:51:33Z","file_id":"13530","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":6023318,"access_level":"open_access","file_name":"Fig4_f_3_top.tif"},{"relation":"main_file","file_id":"13531","checksum":"5d76b2ad7d7acbb1afa27cf52194b1cb","success":1,"date_created":"2023-08-03T11:51:34Z","date_updated":"2023-08-03T11:51:34Z","access_level":"open_access","file_name":"Fig4_f_4_top.tif","file_size":6038186,"content_type":"image/tiff","creator":"jdanzl"},{"access_level":"open_access","file_name":"Fig4_f_5_top.tif","content_type":"image/tiff","file_size":2324158,"creator":"jdanzl","relation":"main_file","file_id":"13532","checksum":"9cee6b3387bd880843f2c6cdfa0d4a8d","success":1,"date_created":"2023-08-03T11:51:34Z","date_updated":"2023-08-03T11:51:34Z"},{"relation":"main_file","file_id":"13533","date_created":"2023-08-03T11:51:34Z","date_updated":"2023-08-03T11:51:34Z","checksum":"a48fc9e1bb2710cf28466ae6d2a20c19","success":1,"file_name":"Fig4_f_1_bottom.png","access_level":"open_access","content_type":"image/png","file_size":1150264,"creator":"jdanzl"},{"success":1,"checksum":"ff47ca30f814e17561974b73b0913a1a","date_created":"2023-08-03T11:51:34Z","date_updated":"2023-08-03T11:51:34Z","file_id":"13534","relation":"main_file","creator":"jdanzl","content_type":"image/png","file_size":1157424,"access_level":"open_access","file_name":"Fig4_f_2_bottom.png"},{"creator":"jdanzl","content_type":"image/png","file_size":1152715,"file_name":"Fig4_f_3_bottom.png","access_level":"open_access","date_created":"2023-08-03T11:51:34Z","date_updated":"2023-08-03T11:51:34Z","success":1,"checksum":"2ea3fb8d79cee8ae9de7ca31d003910f","file_id":"13535","relation":"main_file"},{"file_id":"13536","relation":"main_file","date_updated":"2023-08-03T11:51:34Z","date_created":"2023-08-03T11:51:34Z","success":1,"checksum":"d25e5ecf2f0c3be7c20a7e25a7a334b8","file_name":"Fig4_f_4_bottom.png","access_level":"open_access","creator":"jdanzl","content_type":"image/png","file_size":1105240},{"file_name":"Fig4_f_5_bottom.png","access_level":"open_access","creator":"jdanzl","file_size":1228157,"content_type":"image/png","file_id":"13537","relation":"main_file","date_updated":"2023-08-03T11:51:35Z","date_created":"2023-08-03T11:51:35Z","success":1,"checksum":"76535f569a57b967e378df89b7d2982a"},{"checksum":"d46fefe73daa3ec08b847631e9de6828","success":1,"date_updated":"2023-08-03T11:53:52Z","date_created":"2023-08-03T11:53:52Z","relation":"main_file","file_id":"13538","file_size":91181184,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Fig5_a_1.tif"},{"creator":"jdanzl","file_size":176024124,"content_type":"image/tiff","file_name":"Fig5_a_2.tif","access_level":"open_access","date_updated":"2023-08-03T11:53:54Z","date_created":"2023-08-03T11:53:54Z","success":1,"checksum":"354c0ba876a33672eca37cd61dac7073","file_id":"13539","relation":"main_file"},{"file_id":"13540","relation":"main_file","success":1,"checksum":"d07925040ef0c3c51edbdca8cf3ec1fa","date_updated":"2023-08-03T11:53:53Z","date_created":"2023-08-03T11:53:53Z","access_level":"open_access","file_name":"Fig5_a_3.tif","creator":"jdanzl","content_type":"image/tiff","file_size":15812494},{"creator":"jdanzl","content_type":"image/tiff","file_size":11117689,"file_name":"Fig5_a_4.tif","access_level":"open_access","date_created":"2023-08-03T11:53:53Z","date_updated":"2023-08-03T11:53:53Z","success":1,"checksum":"7c34b19a1764e2f501f11dcabd442ce2","file_id":"13541","relation":"main_file"},{"relation":"main_file","file_id":"13542","checksum":"c49af4b96532e00b3a51574a2a94decb","success":1,"date_created":"2023-08-03T11:53:53Z","date_updated":"2023-08-03T11:53:53Z","access_level":"open_access","file_name":"Fig5_a_5.tif","content_type":"image/tiff","file_size":111665,"creator":"jdanzl"},{"creator":"jdanzl","file_size":16676449,"content_type":"image/tiff","file_name":"Fig5_b_1.tif","access_level":"open_access","date_created":"2023-08-03T11:53:54Z","date_updated":"2023-08-03T11:53:54Z","success":1,"checksum":"1b42eadcdd71f2dc00d82784673aa577","file_id":"13543","relation":"main_file"},{"creator":"jdanzl","content_type":"image/tiff","file_size":427651,"file_name":"Fig5_b_2-4.tif","access_level":"open_access","date_updated":"2023-08-03T11:53:54Z","date_created":"2023-08-03T11:53:54Z","success":1,"checksum":"0d27266bf216f71fcb5496731ce76dd5","file_id":"13544","relation":"main_file"},{"date_updated":"2023-08-03T11:53:55Z","date_created":"2023-08-03T11:53:55Z","checksum":"c4232930068a0a8f7c8a70ce0ea48dc2","success":1,"relation":"main_file","file_id":"13545","file_size":11117705,"content_type":"image/tiff","creator":"jdanzl","file_name":"Fig5_c_1-3.tif","access_level":"open_access"},{"content_type":"image/tiff","file_size":254153,"creator":"jdanzl","file_name":"Fig5_c_2-4.tif","access_level":"open_access","date_created":"2023-08-03T11:53:55Z","date_updated":"2023-08-03T11:53:55Z","checksum":"c9caad749be0ef04ef8013cfdb69f8fc","success":1,"relation":"main_file","file_id":"13546"},{"file_id":"13547","relation":"main_file","date_created":"2023-08-03T11:54:14Z","date_updated":"2023-08-03T11:54:14Z","success":1,"checksum":"5c6d36b96bde1e090cc428b269aba6ec","file_name":"Fig5_e_1.tif","access_level":"open_access","creator":"jdanzl","file_size":737290746,"content_type":"image/tiff"},{"creator":"jdanzl","content_type":"image/tiff","file_size":31366288,"file_name":"Fig5_e_2.tif","access_level":"open_access","date_updated":"2023-08-03T11:53:57Z","date_created":"2023-08-03T11:53:57Z","success":1,"checksum":"f97632984b6a73e06cc0c8c947cdcc71","file_id":"13548","relation":"main_file"},{"date_updated":"2023-08-03T11:53:57Z","date_created":"2023-08-03T11:53:57Z","success":1,"checksum":"8c471eeaa59008eed9e91afe5e1f1698","file_id":"13549","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":1216304,"file_name":"Fig5_e_3.tif","access_level":"open_access"},{"date_created":"2023-08-03T11:53:59Z","date_updated":"2023-08-03T11:53:59Z","checksum":"31c10c3a1d874b5028e35e2dd8800a68","success":1,"relation":"main_file","file_id":"13550","content_type":"image/tiff","file_size":35669193,"creator":"jdanzl","file_name":"Fig5_g.tif","access_level":"open_access"},{"checksum":"0e9fe7b7ee06a630837e9d3c41b80e01","success":1,"date_created":"2023-08-03T11:53:59Z","date_updated":"2023-08-03T11:53:59Z","relation":"main_file","file_id":"13551","content_type":"image/tiff","file_size":7492349,"creator":"jdanzl","access_level":"open_access","file_name":"Fig5_h.tif"},{"content_type":"image/tiff","file_size":121446390,"creator":"jdanzl","access_level":"open_access","file_name":"Fig6_a_1.tif","checksum":"d25b3d04bb8364089cafdf8deb6846b6","success":1,"date_updated":"2023-08-03T11:59:18Z","date_created":"2023-08-03T11:59:18Z","relation":"main_file","file_id":"13552"},{"success":1,"checksum":"4361e10db6ec48c2d4579ac089ff3ca3","date_updated":"2023-08-03T11:59:22Z","date_created":"2023-08-03T11:59:22Z","file_id":"13553","relation":"main_file","creator":"jdanzl","file_size":264412158,"content_type":"image/tiff","access_level":"open_access","file_name":"Fig6_a_2.tif"},{"content_type":"image/tiff","file_size":57399051,"creator":"jdanzl","file_name":"Fig6_a_3.tif","access_level":"open_access","date_updated":"2023-08-03T11:59:21Z","date_created":"2023-08-03T11:59:21Z","checksum":"fe3a00ae75fb3388438e53ced92a9498","success":1,"relation":"main_file","file_id":"13554"},{"date_updated":"2023-08-03T11:59:21Z","date_created":"2023-08-03T11:59:21Z","success":1,"checksum":"4372c13d6dd2274aa749799b8e96bf06","file_id":"13555","relation":"main_file","creator":"jdanzl","file_size":6003163,"content_type":"image/tiff","file_name":"Fig6_b_left.tif","access_level":"open_access"},{"file_name":"Fig6_b_right.tif","access_level":"open_access","creator":"jdanzl","file_size":511265,"content_type":"image/tiff","file_id":"13556","relation":"main_file","date_updated":"2023-08-03T11:59:21Z","date_created":"2023-08-03T11:59:21Z","success":1,"checksum":"f7ae4e50f2f4d414c598bddd691b722a"},{"file_id":"13557","relation":"main_file","success":1,"checksum":"cde658a9ca666bdef1b29613ab72092d","date_created":"2023-08-03T11:59:39Z","date_updated":"2023-08-03T11:59:39Z","access_level":"open_access","file_name":"Fig6_c_n2v.tif","creator":"jdanzl","content_type":"image/tiff","file_size":467125933},{"content_type":"image/tiff","file_size":322251440,"creator":"jdanzl","file_name":"Fig6_c.tif","access_level":"open_access","date_updated":"2023-08-03T11:59:36Z","date_created":"2023-08-03T11:59:36Z","checksum":"57e73c1fedcee4bd8ab62518baa0e0e5","success":1,"relation":"main_file","file_id":"13558"},{"file_id":"13559","relation":"main_file","success":1,"checksum":"53175073b83ab569241ae64ef7f180b3","date_updated":"2023-08-03T11:59:38Z","date_created":"2023-08-03T11:59:38Z","access_level":"open_access","file_name":"Fig6_d_1.tif","creator":"jdanzl","content_type":"image/tiff","file_size":37641738},{"checksum":"af97423e1077af85465a5e39fa25d1e4","success":1,"date_updated":"2023-08-03T11:59:40Z","date_created":"2023-08-03T11:59:40Z","relation":"main_file","file_id":"13560","content_type":"image/tiff","file_size":70596929,"creator":"jdanzl","access_level":"open_access","file_name":"Fig6_d_2.tif"},{"success":1,"checksum":"f1fd7a9fa2a12a930fe35bdf53ba3ff7","date_updated":"2023-08-03T11:59:42Z","date_created":"2023-08-03T11:59:42Z","file_id":"13561","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":15303146,"access_level":"open_access","file_name":"Fig6_d_3.tif"},{"date_updated":"2023-08-03T11:59:41Z","date_created":"2023-08-03T11:59:41Z","checksum":"95655df5cdb05f09912298de9a3711b4","success":1,"relation":"main_file","file_id":"13562","file_size":893051,"content_type":"image/tiff","creator":"jdanzl","file_name":"Fig6_d_4.tif","access_level":"open_access"},{"access_level":"open_access","file_name":"Fig6_e.tif","content_type":"image/tiff","file_size":381232200,"creator":"jdanzl","relation":"main_file","file_id":"13563","checksum":"4d722b4e09ce617252d284f78ea2ee30","success":1,"date_updated":"2023-08-03T11:59:58Z","date_created":"2023-08-03T11:59:58Z"},{"file_name":"Fig6_f.tif","access_level":"open_access","content_type":"image/tiff","file_size":345804470,"creator":"jdanzl","relation":"main_file","file_id":"13564","date_updated":"2023-08-03T11:59:58Z","date_created":"2023-08-03T11:59:58Z","checksum":"d1eda9599f422a614404dd112f0c3185","success":1},{"access_level":"open_access","file_name":"Fig6_g.tif","creator":"jdanzl","content_type":"image/tiff","file_size":346119927,"file_id":"13565","relation":"main_file","success":1,"checksum":"1cd202570f964b78e1f55c987d5d5de1","date_created":"2023-08-03T12:00:08Z","date_updated":"2023-08-03T12:00:08Z"},{"date_created":"2023-08-03T12:00:02Z","date_updated":"2023-08-03T12:00:02Z","success":1,"checksum":"78cbf1906ff8746ed524c62f8f580f52","file_id":"13566","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":7064977,"file_name":"Fig6_h.tif","access_level":"open_access"},{"content_type":"image/tiff","file_size":7110129,"creator":"jdanzl","file_name":"Fig6_i.tif","access_level":"open_access","date_created":"2023-08-03T12:00:03Z","date_updated":"2023-08-03T12:00:03Z","checksum":"21635ce59f1ae5a334aaad3f4c0b171c","success":1,"relation":"main_file","file_id":"13567"},{"content_type":"image/tiff","file_size":110297540,"creator":"jdanzl","file_name":"Fig6_j.tif","access_level":"open_access","date_updated":"2023-08-03T12:00:07Z","date_created":"2023-08-03T12:00:07Z","checksum":"704962856f2a024ce1f321a1785556e3","success":1,"relation":"main_file","file_id":"13568"},{"relation":"main_file","file_id":"13569","checksum":"4b74ba5ae1e2bb2201bf51c74d733268","success":1,"date_created":"2023-08-03T12:00:07Z","date_updated":"2023-08-03T12:00:07Z","access_level":"open_access","file_name":"Fig6_k.tif","file_size":11117689,"content_type":"image/tiff","creator":"jdanzl"},{"access_level":"open_access","file_name":"Extdata_01_a_1.tif","creator":"jdanzl","file_size":1287107,"content_type":"image/tiff","file_id":"13570","relation":"main_file","success":1,"checksum":"a8efa3007df4e9e0f80c7a2bbc451178","date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z"},{"checksum":"7f24b6c84ce96a67347eb5ef4c43fac1","success":1,"date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z","relation":"main_file","file_id":"13571","file_size":58307,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Extdata_01_a_2.tif"},{"content_type":"image/tiff","file_size":1283209,"creator":"jdanzl","access_level":"open_access","file_name":"Extdata_01_a_3.tif","checksum":"fa7ae4eefc11b9b60c4ab80f6e839b73","success":1,"date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z","relation":"main_file","file_id":"13572"},{"checksum":"cec9a5eb87f1b56e64626a00c6d0e53d","success":1,"date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z","relation":"main_file","file_id":"13573","file_size":54409,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Extdata_01_a_4.tif"},{"relation":"main_file","file_id":"13574","date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z","checksum":"450e4786d66d5059028420153908a4dc","success":1,"file_name":"Extdata_01_c_1.png","access_level":"open_access","file_size":2431,"content_type":"image/png","creator":"jdanzl"},{"file_id":"13575","relation":"main_file","date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z","success":1,"checksum":"feb758609b399cf644dd3ae7cd75fb96","file_name":"Extdata_01_c_2.png","access_level":"open_access","creator":"jdanzl","file_size":612,"content_type":"image/png"},{"content_type":"image/tiff","file_size":433981,"creator":"jdanzl","access_level":"open_access","file_name":"Extdata_01_d_1.tif","checksum":"52f43300a50f83f9a6ff1409324086ee","success":1,"date_updated":"2023-08-03T12:05:41Z","date_created":"2023-08-03T12:05:41Z","relation":"main_file","file_id":"13576"},{"creator":"jdanzl","file_size":19649,"content_type":"image/tiff","file_name":"Extdata_01_d_2.tif","access_level":"open_access","date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z","success":1,"checksum":"28925dcf8792f524e286beb16b0c532c","file_id":"13577","relation":"main_file"},{"file_id":"13578","relation":"main_file","date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z","success":1,"checksum":"c93b2f0426c80d18e252435f1e146568","file_name":"Extdata_01_d_3.tif","access_level":"open_access","creator":"jdanzl","file_size":433981,"content_type":"image/tiff"},{"date_updated":"2023-08-03T12:05:41Z","date_created":"2023-08-03T12:05:41Z","checksum":"c0ed722a768001431f47646ef98c44da","success":1,"relation":"main_file","file_id":"13579","file_size":19649,"content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata_01_d_4.tif","access_level":"open_access"},{"file_id":"13580","relation":"main_file","date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z","success":1,"checksum":"db9d386276b985b8e702f797e910bd0b","file_name":"Extdata_01_f_1.png","access_level":"open_access","creator":"jdanzl","file_size":4752,"content_type":"image/png"},{"relation":"main_file","file_id":"13581","date_created":"2023-08-03T12:05:41Z","date_updated":"2023-08-03T12:05:41Z","checksum":"244f6645d191c50d997c0dc6a15535ec","success":1,"file_name":"Extdata_01_f_2.png","access_level":"open_access","content_type":"image/png","file_size":2698,"creator":"jdanzl"},{"checksum":"3d6100b836d850b38e66e0f5a7919495","success":1,"date_updated":"2023-08-03T12:07:27Z","date_created":"2023-08-03T12:07:27Z","relation":"main_file","file_id":"13582","content_type":"image/tiff","file_size":24003247,"creator":"jdanzl","access_level":"open_access","file_name":"Extdata_02_a.tif"},{"creator":"jdanzl","file_size":1164723,"content_type":"image/tiff","access_level":"open_access","file_name":"Extdata_02_b.tif","success":1,"checksum":"3cd6a3b7bf2061cf5c7810c0001236e5","date_updated":"2023-08-03T12:07:27Z","date_created":"2023-08-03T12:07:27Z","file_id":"13583","relation":"main_file"},{"date_updated":"2023-08-03T12:10:32Z","date_created":"2023-08-03T12:10:32Z","checksum":"d87c82d663ffea379cc86c56981e2e16","success":1,"relation":"main_file","file_id":"13584","file_size":7109763,"content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata_03_SYPH1_xy_1.tif","access_level":"open_access"},{"checksum":"a3703d0d26393578d4ba466681fc6be8","success":1,"date_created":"2023-08-03T12:10:32Z","date_updated":"2023-08-03T12:10:32Z","relation":"main_file","file_id":"13585","file_size":27791,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Extdata_03_SYPH1_xz_1-2.tif"},{"file_size":27721,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Extdata_03_SYPH1_xz_3-4.tif","checksum":"f653ac7d531cf9896cbd6a9edf2a7759","success":1,"date_updated":"2023-08-03T12:10:32Z","date_created":"2023-08-03T12:10:32Z","relation":"main_file","file_id":"13586"},{"relation":"main_file","file_id":"13587","date_created":"2023-08-03T12:10:33Z","date_updated":"2023-08-03T12:10:33Z","checksum":"04edbb337df4d5470e033a4f53c07300","success":1,"file_name":"Extdata_03_SYPH1_xz_n2v.tif","access_level":"open_access","content_type":"image/tiff","file_size":57657482,"creator":"jdanzl"},{"checksum":"09e1fe2dd3a1e8349693fda55d0985ca","success":1,"date_updated":"2023-08-03T12:10:34Z","date_created":"2023-08-03T12:10:34Z","relation":"main_file","file_id":"13588","file_size":57657527,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Extdata_03_SYPH1_xz.tif"},{"access_level":"open_access","file_name":"Extdata_03_SYP1_xy_1.tif","content_type":"image/tiff","file_size":7109763,"creator":"jdanzl","relation":"main_file","file_id":"13589","checksum":"458f8d486cb63876c6664cde4a9be27c","success":1,"date_updated":"2023-08-03T12:12:25Z","date_created":"2023-08-03T12:12:25Z"},{"file_name":"Extdata_03_SYP1_xy_2-3.tif","access_level":"open_access","creator":"jdanzl","file_size":72963,"content_type":"image/tiff","file_id":"13590","relation":"main_file","date_updated":"2023-08-03T12:12:25Z","date_created":"2023-08-03T12:12:25Z","success":1,"checksum":"e2dfccc882d85b660d65a596e1a8cbac"},{"creator":"jdanzl","file_size":27724,"content_type":"image/tiff","access_level":"open_access","file_name":"Extdata_03_SYP1_xz_1-2.tif","success":1,"checksum":"a331be6052c68cc027cdb79f15447d9a","date_updated":"2023-08-03T12:12:25Z","date_created":"2023-08-03T12:12:25Z","file_id":"13591","relation":"main_file"},{"content_type":"image/tiff","file_size":27724,"creator":"jdanzl","access_level":"open_access","file_name":"Extdata_03_SYP1_xz_3-4.tif","checksum":"4de626b035b43feaacd923fca411e6a2","success":1,"date_updated":"2023-08-03T12:12:25Z","date_created":"2023-08-03T12:12:25Z","relation":"main_file","file_id":"13592"},{"date_updated":"2023-08-03T12:12:30Z","date_created":"2023-08-03T12:12:30Z","checksum":"d8076e927a074f024924d9bdb6fdd5e6","success":1,"relation":"main_file","file_id":"13593","content_type":"image/tiff","file_size":214613644,"creator":"jdanzl","file_name":"Extdata_03_SYP1_xz_n2v.tif","access_level":"open_access"},{"date_created":"2023-08-03T12:12:30Z","date_updated":"2023-08-03T12:12:30Z","success":1,"checksum":"79cf7d0f450c397caa877dd06b52f8df","file_id":"13594","relation":"main_file","creator":"jdanzl","file_size":214613687,"content_type":"image/tiff","file_name":"Extdata_03_SYP1_xz.tif","access_level":"open_access"},{"success":1,"checksum":"60ac6ea988d9b8bdaba2f09e0e1ef370","date_updated":"2023-08-03T12:14:29Z","date_created":"2023-08-03T12:14:29Z","file_id":"13595","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":7109747,"access_level":"open_access","file_name":"Extdata03_VAMP2_xy_1.tif"},{"access_level":"open_access","file_name":"Extdata03_VAMP2_xy_2-3.tif","creator":"jdanzl","file_size":72947,"content_type":"image/tiff","file_id":"13596","relation":"main_file","success":1,"checksum":"92bf312da2dd356aa4fc9a0e7d811af2","date_created":"2023-08-03T12:14:29Z","date_updated":"2023-08-03T12:14:29Z"},{"file_id":"13597","relation":"main_file","date_created":"2023-08-03T12:14:29Z","date_updated":"2023-08-03T12:14:29Z","success":1,"checksum":"9319e293092eeee24e3c658fc178af1a","file_name":"Extdata03_VAMP2_xz_1-2.tif","access_level":"open_access","creator":"jdanzl","file_size":27724,"content_type":"image/tiff"},{"relation":"main_file","file_id":"13598","checksum":"a627d7fd9a89bdde968e6d9bff846254","success":1,"date_updated":"2023-08-03T12:14:29Z","date_created":"2023-08-03T12:14:29Z","access_level":"open_access","file_name":"Extdata03_VAMP2_xz_3-4.tif","file_size":27708,"content_type":"image/tiff","creator":"jdanzl"},{"file_id":"13599","relation":"main_file","success":1,"checksum":"7a5363a185edce59ce57535f7d855092","date_created":"2023-08-03T12:14:32Z","date_updated":"2023-08-03T12:14:32Z","access_level":"open_access","file_name":"Extdata03_VAMP2_xz_n2v.tif","creator":"jdanzl","file_size":102457485,"content_type":"image/tiff"},{"creator":"jdanzl","file_size":102457527,"content_type":"image/tiff","file_name":"Extdata03_VAMP2_xz.tif","access_level":"open_access","date_created":"2023-08-03T12:14:32Z","date_updated":"2023-08-03T12:14:32Z","success":1,"checksum":"8e7fc4d00fff8ec6d9340e969f37e176","file_id":"13600","relation":"main_file"},{"access_level":"open_access","file_name":"Extdata04_VGLUT_xy_1.tif","content_type":"image/tiff","file_size":11117763,"creator":"jdanzl","relation":"main_file","file_id":"13601","checksum":"be17472f73ed8cd02a700b861f1dbac0","success":1,"date_created":"2023-08-03T12:16:08Z","date_updated":"2023-08-03T12:16:08Z"},{"content_type":"image/tiff","file_size":72947,"creator":"jdanzl","file_name":"Extdata04_VGLUT_xy_2-3.tif","access_level":"open_access","date_updated":"2023-08-03T12:16:08Z","date_created":"2023-08-03T12:16:08Z","checksum":"dbc3ec477f491ce3e98ab40475bd90ca","success":1,"relation":"main_file","file_id":"13602"},{"access_level":"open_access","file_name":"Extdata04_VGLUT_xz_1-2.tif","creator":"jdanzl","file_size":53367,"content_type":"image/tiff","file_id":"13603","relation":"main_file","success":1,"checksum":"8ee877e6b843bb0e38febb0481962d69","date_created":"2023-08-03T12:16:08Z","date_updated":"2023-08-03T12:16:08Z"},{"access_level":"open_access","file_name":"Extdata04_VGLUT_xz_3-4.tif","content_type":"image/tiff","file_size":53358,"creator":"jdanzl","relation":"main_file","file_id":"13604","checksum":"9873f54d50955a2c204d0559c87aa13d","success":1,"date_updated":"2023-08-03T12:16:08Z","date_created":"2023-08-03T12:16:08Z"},{"creator":"jdanzl","content_type":"image/tiff","file_size":144076208,"file_name":"Extdata04_VGLUT_xz_n2v.tif","access_level":"open_access","date_updated":"2023-08-03T12:16:11Z","date_created":"2023-08-03T12:16:11Z","success":1,"checksum":"8eda08ae5981fcfa3758d0320b1923af","file_id":"13605","relation":"main_file"},{"checksum":"bc0b10229d498f60996a5b05cb87c428","success":1,"date_created":"2023-08-03T12:16:10Z","date_updated":"2023-08-03T12:16:10Z","relation":"main_file","file_id":"13606","file_size":72071440,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Extdata04_VGLUT_xz.tif"},{"file_name":"Extdata03_VGAT_xy_1.tif","access_level":"open_access","content_type":"image/tiff","file_size":7109763,"creator":"jdanzl","relation":"main_file","file_id":"13607","date_created":"2023-08-03T12:32:32Z","date_updated":"2023-08-03T12:32:32Z","checksum":"e8d093ce7d98ea5647d8a80e3084c40b","success":1},{"creator":"jdanzl","content_type":"image/tiff","file_size":72947,"file_name":"Extdata03_VGAT_xy_2-3.tif","access_level":"open_access","date_updated":"2023-08-03T12:32:32Z","date_created":"2023-08-03T12:32:32Z","success":1,"checksum":"36590ca7a586de7ad76d3ad659c38995","file_id":"13608","relation":"main_file"},{"checksum":"3914c7709f69d4d18f23a9fb6dc64572","success":1,"date_created":"2023-08-03T12:32:32Z","date_updated":"2023-08-03T12:32:32Z","relation":"main_file","file_id":"13609","content_type":"image/tiff","file_size":53355,"creator":"jdanzl","access_level":"open_access","file_name":"Extdata03_VGAT_xz_1-2.tif"},{"access_level":"open_access","file_name":"Extdata03_VGAT_xz_3-4.tif","creator":"jdanzl","file_size":53339,"content_type":"image/tiff","file_id":"13610","relation":"main_file","success":1,"checksum":"fa98aa521adaefb9e4476fe519e88e59","date_created":"2023-08-03T12:32:32Z","date_updated":"2023-08-03T12:32:32Z"},{"content_type":"image/tiff","file_size":204861338,"creator":"jdanzl","file_name":"Extdata03_VGAT_xz_n2v.tif","access_level":"open_access","date_created":"2023-08-03T12:32:36Z","date_updated":"2023-08-03T12:32:36Z","checksum":"cd6f384c51756977e37f45043c913252","success":1,"relation":"main_file","file_id":"13611"},{"relation":"main_file","file_id":"13612","date_updated":"2023-08-03T12:32:35Z","date_created":"2023-08-03T12:32:35Z","checksum":"fb7e46feda5ac07877a91ad963da0e31","success":1,"file_name":"Extdata03_VGAT_xz.tif","access_level":"open_access","content_type":"image/tiff","file_size":102457527,"creator":"jdanzl"},{"access_level":"open_access","file_name":"Extdata04_MUNC_xy_1.png","creator":"jdanzl","file_size":4348932,"content_type":"image/png","file_id":"13613","relation":"main_file","success":1,"checksum":"fe033e0a377d391cd8e4af04987d9900","date_updated":"2023-08-03T12:36:46Z","date_created":"2023-08-03T12:36:46Z"},{"relation":"main_file","file_id":"13614","date_updated":"2023-08-03T12:35:58Z","date_created":"2023-08-03T12:35:58Z","checksum":"771d8e2b9192b6620f54ab30e4fad594","success":1,"file_name":"Extdata04_MUNC_xy_2-3.tif","access_level":"open_access","file_size":72947,"content_type":"image/tiff","creator":"jdanzl"},{"date_updated":"2023-08-03T12:35:58Z","date_created":"2023-08-03T12:35:58Z","success":1,"checksum":"4256d8f06fa2077b352776cf76b46618","file_id":"13615","relation":"main_file","creator":"jdanzl","file_size":53435,"content_type":"image/tiff","file_name":"Extdata04_MUNC_xz_1-2.tif","access_level":"open_access"},{"date_updated":"2023-08-03T12:35:58Z","date_created":"2023-08-03T12:35:58Z","success":1,"checksum":"0b9365638c81d545e773170cf04b04b8","file_id":"13616","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":55941,"file_name":"Extdata04_MUNC_xz_3-4.tif","access_level":"open_access"},{"creator":"jdanzl","file_size":204861404,"content_type":"image/tiff","access_level":"open_access","file_name":"Extdata04_MUNC_xz_n2v.tif","success":1,"checksum":"9fa62688dab03c592cec47bf5d1c304d","date_updated":"2023-08-03T12:36:04Z","date_created":"2023-08-03T12:36:04Z","file_id":"13617","relation":"main_file"},{"relation":"main_file","file_id":"13618","date_updated":"2023-08-03T12:36:02Z","date_created":"2023-08-03T12:36:02Z","checksum":"39f710080a26136dbbe4c8916b434c65","success":1,"file_name":"Extdata04_MUNC_xz.tif","access_level":"open_access","file_size":102457527,"content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","file_size":11117757,"access_level":"open_access","file_name":"Extdata04_BASSOON_xy_1.tif","success":1,"checksum":"eaabc8112645a73f4326e621b4e6ad0a","date_created":"2023-08-03T12:38:39Z","date_updated":"2023-08-03T12:38:39Z","file_id":"13619","relation":"main_file"},{"relation":"main_file","file_id":"13620","checksum":"15f624d29c473ce6e0ae7e466efc059a","success":1,"date_updated":"2023-08-03T12:38:39Z","date_created":"2023-08-03T12:38:39Z","access_level":"open_access","file_name":"Extdata04_BASSOON_xy_2-3.tif","content_type":"image/tiff","file_size":74041,"creator":"jdanzl"},{"relation":"main_file","file_id":"13621","checksum":"1cb4b4d219767a30072c804a07140240","success":1,"date_created":"2023-08-03T12:38:39Z","date_updated":"2023-08-03T12:38:39Z","access_level":"open_access","file_name":"Extdata04_BASSOON_xz_1-2.tif","file_size":53435,"content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","file_size":53362,"access_level":"open_access","file_name":"Extdata04_BASSOON_xz_3-4.tif","success":1,"checksum":"1bac9c192da9375d81cb3747f48c9be4","date_updated":"2023-08-03T12:38:39Z","date_created":"2023-08-03T12:38:39Z","file_id":"13622","relation":"main_file"},{"date_updated":"2023-08-03T12:38:50Z","date_created":"2023-08-03T12:38:50Z","checksum":"57fa91bfb1e2937e3449e57096b6fc74","success":1,"relation":"main_file","file_id":"13623","file_size":576071410,"content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata04_BASSOON_xz_n2v.tif","access_level":"open_access"},{"relation":"main_file","file_id":"13624","checksum":"ac51a734dcf7d1b94afcd4a471f10626","success":1,"date_updated":"2023-08-03T12:38:46Z","date_created":"2023-08-03T12:38:46Z","access_level":"open_access","file_name":"Extdata04_BASSOON_xz.tif","content_type":"image/tiff","file_size":288066640,"creator":"jdanzl"},{"access_level":"open_access","file_name":"Extdata04_HOMER1_xy_1.tif","file_size":7109763,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13625","checksum":"244043f7acba250d560e29123702dcd7","success":1,"date_created":"2023-08-03T12:40:57Z","date_updated":"2023-08-03T12:40:57Z"},{"success":1,"checksum":"eff51ff97539f6491989bfc3d3f88860","date_updated":"2023-08-03T12:40:57Z","date_created":"2023-08-03T12:40:57Z","file_id":"13626","relation":"main_file","creator":"jdanzl","file_size":72963,"content_type":"image/tiff","access_level":"open_access","file_name":"Extdata04_HOMER1_xy_2-3.tif"},{"content_type":"image/tiff","file_size":53375,"creator":"jdanzl","file_name":"Extdata04_HOMER1_xz_1-2.tif","access_level":"open_access","date_updated":"2023-08-03T12:40:57Z","date_created":"2023-08-03T12:40:57Z","checksum":"df35046d9bca4e97a5625c5a55218cfb","success":1,"relation":"main_file","file_id":"13627"},{"creator":"jdanzl","file_size":53359,"content_type":"image/tiff","access_level":"open_access","file_name":"Extdata04_HOMER1_xz_3-4.tif","success":1,"checksum":"5dc7536f7aaea084dfc329537d8412ce","date_updated":"2023-08-03T12:40:57Z","date_created":"2023-08-03T12:40:57Z","file_id":"13628","relation":"main_file"},{"checksum":"0b407669a26b9d7730a41361ceffda9a","success":1,"date_created":"2023-08-03T12:41:00Z","date_updated":"2023-08-03T12:41:00Z","relation":"main_file","file_id":"13629","content_type":"image/tiff","file_size":204861339,"creator":"jdanzl","access_level":"open_access","file_name":"Extdata04_HOMER1_xz_n2v.tif"},{"file_name":"Extdata04_HOMER1_xz.tif","access_level":"open_access","file_size":102457527,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13630","date_created":"2023-08-03T12:40:59Z","date_updated":"2023-08-03T12:40:59Z","checksum":"f95a0da9bea8b605fc2ed310190836b3","success":1},{"file_id":"13631","relation":"main_file","date_updated":"2023-08-03T12:45:08Z","date_created":"2023-08-03T12:45:08Z","success":1,"checksum":"cf74307696548e7aa90e59a0935a74a1","file_name":"Extdata04_SHANK2_xy_1.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":7120361},{"file_id":"13632","relation":"main_file","date_updated":"2023-08-03T12:45:08Z","date_created":"2023-08-03T12:45:08Z","success":1,"checksum":"7b3490246df4f5bb68d0df682161820f","file_name":"Extdata04_SHANK2_xy_2-3.tif","access_level":"open_access","creator":"jdanzl","file_size":72973,"content_type":"image/tiff"},{"file_size":53443,"content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata04_SHANK2_xz_1-2.tif","access_level":"open_access","date_updated":"2023-08-03T12:45:08Z","date_created":"2023-08-03T12:45:08Z","checksum":"c599d6830e61c386219e266f4717fe7c","success":1,"relation":"main_file","file_id":"13633"},{"date_updated":"2023-08-03T12:45:08Z","date_created":"2023-08-03T12:45:08Z","success":1,"checksum":"5b9912b2a408d224ad669f39dc8d5bc6","file_id":"13634","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":53362,"file_name":"Extdata04_SHANK2_xz_3-4.tif","access_level":"open_access"},{"date_updated":"2023-08-03T12:45:30Z","date_created":"2023-08-03T12:45:30Z","success":1,"checksum":"17f2064ceeb99cbb51208c27b1961ed5","file_id":"13635","relation":"main_file","creator":"jdanzl","file_size":691291157,"content_type":"image/tiff","file_name":"Extdata04_SHANK2_xz_n2v.tif","access_level":"open_access"},{"content_type":"image/tiff","file_size":518519275,"creator":"jdanzl","access_level":"open_access","file_name":"Extdata04_SHANK2_xz.tif","checksum":"21f1bb8d2a00c88bdd6febe6285acc2a","success":1,"date_updated":"2023-08-03T12:45:26Z","date_created":"2023-08-03T12:45:26Z","relation":"main_file","file_id":"13636"},{"file_id":"13637","relation":"main_file","success":1,"checksum":"47597ca873f1ea81cd4f7e9bfbb34aa4","date_created":"2023-08-03T12:46:25Z","date_updated":"2023-08-03T12:46:25Z","access_level":"open_access","file_name":"Extdata04_GEPHYRIN_xy_1.tif","creator":"jdanzl","content_type":"image/tiff","file_size":752109},{"date_created":"2023-08-03T12:46:25Z","date_updated":"2023-08-03T12:46:25Z","checksum":"14ed7ae64eb783e6b30f824f21959d0f","success":1,"relation":"main_file","file_id":"13638","content_type":"image/tiff","file_size":72909,"creator":"jdanzl","file_name":"Extdata04_GEPHYRIN_xy_2-3.tif","access_level":"open_access"},{"date_created":"2023-08-03T12:46:25Z","date_updated":"2023-08-03T12:46:25Z","success":1,"checksum":"e361e226428d7b23618eed35b1cad080","file_id":"13639","relation":"main_file","creator":"jdanzl","file_size":53375,"content_type":"image/tiff","file_name":"Extdata04_GEPHYRIN_xz_1-2.tif","access_level":"open_access"},{"relation":"main_file","file_id":"13640","date_updated":"2023-08-03T12:46:25Z","date_created":"2023-08-03T12:46:25Z","checksum":"abe7cee22f69fc2bf1a3ff9955ccf45a","success":1,"file_name":"Extdata04_GEPHYRIN_xz_3-4.tif","access_level":"open_access","content_type":"image/tiff","file_size":53360,"creator":"jdanzl"},{"date_created":"2023-08-03T12:46:27Z","date_updated":"2023-08-03T12:46:27Z","checksum":"59b49b451a63c5ac06fa31a17640c1a2","success":1,"relation":"main_file","file_id":"13641","file_size":73174939,"content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata04_GEPHYRIN_xz_n2v.tif","access_level":"open_access"},{"date_created":"2023-08-03T12:46:26Z","date_updated":"2023-08-03T12:46:26Z","success":1,"checksum":"1ee1feea85c8319ebc6c49147502e412","file_id":"13642","relation":"main_file","creator":"jdanzl","file_size":36614327,"content_type":"image/tiff","file_name":"Extdata04_GEPHYRIN_xz.tif","access_level":"open_access"},{"creator":"jdanzl","file_size":2195652677,"content_type":"image/tiff","access_level":"open_access","file_name":"Extdata06_a-b_n2v.tif","success":1,"checksum":"64d9d868ef89c5cfc65cb199aff831b4","date_updated":"2023-08-03T12:52:23Z","date_created":"2023-08-03T12:52:23Z","file_id":"13643","relation":"main_file"},{"date_updated":"2023-08-03T12:51:41Z","date_created":"2023-08-03T12:51:41Z","success":1,"checksum":"df41d97987798715b1f66e6e72be169b","file_id":"13644","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":1097875840,"file_name":"Extdata06_a-b.tif","access_level":"open_access"},{"date_created":"2023-08-03T12:52:57Z","date_updated":"2023-08-03T12:52:57Z","success":1,"checksum":"95a3eaa3b70e964f6871a5765a53659b","file_id":"13645","relation":"main_file","creator":"jdanzl","file_size":3564577,"content_type":"image/tiff","file_name":"Extdata07_01_bottom.tif","access_level":"open_access"},{"success":1,"checksum":"80b41ad0de73fd61eb67a35262627fac","date_updated":"2023-08-03T12:52:57Z","date_created":"2023-08-03T12:52:57Z","file_id":"13646","relation":"main_file","creator":"jdanzl","file_size":11958844,"content_type":"image/tiff","access_level":"open_access","file_name":"Extdata07_01_top.tif"},{"date_created":"2023-08-03T12:54:27Z","date_updated":"2023-08-03T12:54:27Z","checksum":"f694a0f11e2f782702cb4c411941873a","success":1,"relation":"main_file","file_id":"13647","content_type":"image/tiff","file_size":3564581,"creator":"jdanzl","file_name":"Extdata07_2_bottom.tif","access_level":"open_access"},{"date_created":"2023-08-03T12:54:27Z","date_updated":"2023-08-03T12:54:27Z","success":1,"checksum":"b8b1a1339a25806615c0aa7906a032ff","file_id":"13648","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":11973592,"file_name":"Extdata07_2_top.tif","access_level":"open_access"},{"relation":"main_file","file_id":"13649","checksum":"8a24eb623d65d809fe8afbd527528a77","success":1,"date_created":"2023-08-03T12:54:27Z","date_updated":"2023-08-03T12:54:27Z","access_level":"open_access","file_name":"Extdata07_3_bottom.tif","content_type":"image/tiff","file_size":1281901,"creator":"jdanzl"},{"file_size":16569776,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Extdata07_3_top.tif","checksum":"816b35def703bc48d55b0293fcd8212f","success":1,"date_updated":"2023-08-03T12:54:27Z","date_created":"2023-08-03T12:54:27Z","relation":"main_file","file_id":"13650"},{"relation":"main_file","file_id":"13651","checksum":"7f1375ab8cf28cd136e2e44d1fdfd87d","success":1,"date_created":"2023-08-03T12:54:27Z","date_updated":"2023-08-03T12:54:27Z","access_level":"open_access","file_name":"Extdata07_04_bottom.tif","content_type":"image/tiff","file_size":3564595,"creator":"jdanzl"},{"file_name":"Extdata07_04_top.tif","access_level":"open_access","content_type":"image/tiff","file_size":5896404,"creator":"jdanzl","relation":"main_file","file_id":"13652","date_updated":"2023-08-03T12:54:27Z","date_created":"2023-08-03T12:54:27Z","checksum":"b1dbda28e2a010968716c0411568f139","success":1},{"file_id":"13653","relation":"main_file","success":1,"checksum":"d6e96b7c8e6a7084c14eda83a4dd778a","date_updated":"2023-08-03T12:54:28Z","date_created":"2023-08-03T12:54:28Z","access_level":"open_access","file_name":"Extdata07_5_bottom.tif","creator":"jdanzl","content_type":"image/tiff","file_size":3564609},{"content_type":"image/tiff","file_size":15555618,"creator":"jdanzl","file_name":"Extdata07_5_top.tif","access_level":"open_access","date_updated":"2023-08-03T12:54:28Z","date_created":"2023-08-03T12:54:28Z","checksum":"a647f89a3048f71512cc58c9e53ec022","success":1,"relation":"main_file","file_id":"13654"},{"file_id":"13655","relation":"main_file","success":1,"checksum":"66becb046fe591914df572617d720202","date_created":"2023-08-03T12:54:28Z","date_updated":"2023-08-03T12:54:28Z","access_level":"open_access","file_name":"Extdata07_6_bottom.tif","creator":"jdanzl","file_size":3564577,"content_type":"image/tiff"},{"file_name":"Extdata07_6_top.tif","access_level":"open_access","file_size":6038186,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13656","date_updated":"2023-08-03T12:54:28Z","date_created":"2023-08-03T12:54:28Z","checksum":"5d76b2ad7d7acbb1afa27cf52194b1cb","success":1},{"file_name":"Extdata07_7_bottom.tif","access_level":"open_access","file_size":3560937,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13657","date_created":"2023-08-03T12:54:28Z","date_updated":"2023-08-03T12:54:28Z","checksum":"7f137bdea0f0acb3db75c983ce44ab44","success":1},{"creator":"jdanzl","content_type":"image/tiff","file_size":12031850,"access_level":"open_access","file_name":"Extdata07_7_top.tif","success":1,"checksum":"479e26106280eb61a2d74e29137a2e43","date_created":"2023-08-03T12:54:28Z","date_updated":"2023-08-03T12:54:28Z","file_id":"13658","relation":"main_file"},{"access_level":"open_access","file_name":"Extdata07_8_bottom.tif","content_type":"image/tiff","file_size":3560937,"creator":"jdanzl","relation":"main_file","file_id":"13659","checksum":"8426197ad283c1bc83e3193f323d5f5a","success":1,"date_updated":"2023-08-03T12:54:28Z","date_created":"2023-08-03T12:54:28Z"},{"creator":"jdanzl","file_size":22877176,"content_type":"image/tiff","access_level":"open_access","file_name":"Extdata07_8_top.tif","success":1,"checksum":"b9f75a82b564e1c5c363a495d078f6b7","date_updated":"2023-08-03T12:54:28Z","date_created":"2023-08-03T12:54:28Z","file_id":"13660","relation":"main_file"},{"creator":"jdanzl","file_size":3564605,"content_type":"image/tiff","file_name":"Extdata07_9_bottom.tif","access_level":"open_access","date_updated":"2023-08-03T12:54:28Z","date_created":"2023-08-03T12:54:28Z","success":1,"checksum":"7f1a9f3bb9611362995ae832b2607f4d","file_id":"13661","relation":"main_file"},{"success":1,"checksum":"40286bcb876b655d7421534d0215e86b","date_updated":"2023-08-03T12:54:28Z","date_created":"2023-08-03T12:54:28Z","file_id":"13662","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":2744190,"access_level":"open_access","file_name":"Extdata07_9_top.tif"},{"relation":"main_file","file_id":"13663","date_updated":"2023-08-03T13:00:03Z","date_created":"2023-08-03T13:00:03Z","checksum":"4af220986cc94d412c2354aa7e6c8cce","success":1,"file_name":"Extdata08_6_left.png","access_level":"open_access","content_type":"image/png","file_size":7243611,"creator":"jdanzl"},{"file_name":"Extdata08_6_right.tif","access_level":"open_access","content_type":"image/tiff","file_size":5562487,"creator":"jdanzl","relation":"main_file","file_id":"13664","date_created":"2023-08-03T13:00:03Z","date_updated":"2023-08-03T13:00:03Z","checksum":"af9c2299f1005a3ccc39f41082cea642","success":1},{"file_size":34745956,"content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata08_7_left.tif","access_level":"open_access","date_updated":"2023-08-03T13:00:04Z","date_created":"2023-08-03T13:00:04Z","checksum":"63ef28a174215c70e31d2cabf67d90fc","success":1,"relation":"main_file","file_id":"13665"},{"date_updated":"2023-08-03T13:00:03Z","date_created":"2023-08-03T13:00:03Z","success":1,"checksum":"46de918dfe5f3c23e497ee3daa608dd9","file_id":"13666","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":5562483,"file_name":"Extdata08_7_right.tif","access_level":"open_access"},{"creator":"jdanzl","file_size":31506150,"content_type":"image/tiff","file_name":"Extdata08_b_1_left.tif","access_level":"open_access","date_created":"2023-08-03T13:00:04Z","date_updated":"2023-08-03T13:00:04Z","success":1,"checksum":"8243a7985d2902446183adc35519a0c8","file_id":"13667","relation":"main_file"},{"access_level":"open_access","file_name":"Extdata08_b_1_right.tif","creator":"jdanzl","content_type":"image/tiff","file_size":11117705,"file_id":"13668","relation":"main_file","success":1,"checksum":"857cffa31569efcd5917f9802a106d58","date_updated":"2023-08-03T13:00:04Z","date_created":"2023-08-03T13:00:04Z"},{"file_id":"13669","relation":"main_file","date_created":"2023-08-03T13:00:04Z","date_updated":"2023-08-03T13:00:04Z","success":1,"checksum":"e34902de9119d2cd9f1910f610627c5f","file_name":"Extdata08_b_2_left.png","access_level":"open_access","creator":"jdanzl","content_type":"image/png","file_size":3540493},{"creator":"jdanzl","content_type":"image/tiff","file_size":5562553,"access_level":"open_access","file_name":"Extdata08_b_2_right.tif","success":1,"checksum":"1247d636ac5af22e8950ffd2ff213fea","date_updated":"2023-08-03T13:00:05Z","date_created":"2023-08-03T13:00:05Z","file_id":"13670","relation":"main_file"},{"access_level":"open_access","file_name":"Extdata08_b_3_left.png","file_size":3541547,"content_type":"image/png","creator":"jdanzl","relation":"main_file","file_id":"13671","checksum":"f47c2d3f6824e08853ae2631f6ee7c5f","success":1,"date_updated":"2023-08-03T13:00:05Z","date_created":"2023-08-03T13:00:05Z"},{"file_size":5562553,"content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata08_b_3_right.tif","access_level":"open_access","date_updated":"2023-08-03T13:00:05Z","date_created":"2023-08-03T13:00:05Z","checksum":"1247d636ac5af22e8950ffd2ff213fea","success":1,"relation":"main_file","file_id":"13672"},{"file_id":"13673","relation":"main_file","date_created":"2023-08-03T13:00:05Z","date_updated":"2023-08-03T13:00:05Z","success":1,"checksum":"e90a95ec902a683678acd70336852fd9","file_name":"Extdata08_b_4_left.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":25819844},{"access_level":"open_access","file_name":"Extdata08_b_4_right.tif","file_size":5561131,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13674","checksum":"94cd44cd1c8c7a87ffd5861bf88561a1","success":1,"date_updated":"2023-08-03T13:00:05Z","date_created":"2023-08-03T13:00:05Z"},{"checksum":"fd4ca56dc5c48ad4546df22daef04efc","success":1,"date_created":"2023-08-03T13:00:06Z","date_updated":"2023-08-03T13:00:06Z","relation":"main_file","file_id":"13675","file_size":38786287,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Extdata08_b_5_left.tif"},{"file_id":"13676","relation":"main_file","success":1,"checksum":"52c04890160c4e0f23d3fe2d3ed0c8e7","date_updated":"2023-08-03T13:00:06Z","date_created":"2023-08-03T13:00:06Z","access_level":"open_access","file_name":"Extdata08_b_5_right.tif","creator":"jdanzl","content_type":"image/tiff","file_size":5562487},{"file_size":2133527,"content_type":"image/png","creator":"jdanzl","access_level":"open_access","file_name":"Extdata08_a_1-1.png","checksum":"47ee6f4b083130d5bb720bcf8a832443","success":1,"date_updated":"2023-08-03T13:04:52Z","date_created":"2023-08-03T13:04:52Z","relation":"main_file","file_id":"13677"},{"file_id":"13678","relation":"main_file","date_updated":"2023-08-03T13:04:52Z","date_created":"2023-08-03T13:04:52Z","success":1,"checksum":"5ca2279c372b3b7dbce3ea5658a68399","file_name":"Extdata08_a_1-2.png","access_level":"open_access","creator":"jdanzl","file_size":1782607,"content_type":"image/png"},{"content_type":"image/png","file_size":5117469,"creator":"jdanzl","file_name":"Extdata08_a_2.png","access_level":"open_access","date_created":"2023-08-03T13:04:52Z","date_updated":"2023-08-03T13:04:52Z","checksum":"34b7781e3e4abf9f30a0c85db156b6d5","success":1,"relation":"main_file","file_id":"13679"},{"content_type":"image/png","file_size":6911658,"creator":"jdanzl","access_level":"open_access","file_name":"Extdata08_a_3.png","checksum":"25717007393682098d2208bd0676a0f7","success":1,"date_updated":"2023-08-03T13:04:52Z","date_created":"2023-08-03T13:04:52Z","relation":"main_file","file_id":"13680"},{"file_id":"13681","relation":"main_file","date_updated":"2023-08-03T13:04:53Z","date_created":"2023-08-03T13:04:53Z","success":1,"checksum":"b4b003809acec33c71628066744c7ea9","file_name":"Extdata08_a_4.png","access_level":"open_access","creator":"jdanzl","file_size":8579427,"content_type":"image/png"},{"file_id":"13682","relation":"main_file","date_updated":"2023-08-03T13:04:53Z","date_created":"2023-08-03T13:04:53Z","success":1,"checksum":"4410976cf867ecacf8f197dbd28cc5a0","file_name":"Extdata08_a_5.png","access_level":"open_access","creator":"jdanzl","content_type":"image/png","file_size":9675065},{"date_updated":"2023-08-03T13:04:53Z","date_created":"2023-08-03T13:04:53Z","checksum":"a235dd204f28d3323883e044b031b984","success":1,"relation":"main_file","file_id":"13683","file_size":9769588,"content_type":"image/png","creator":"jdanzl","file_name":"Extdata08_a_6.png","access_level":"open_access"},{"content_type":"image/png","file_size":9114791,"creator":"jdanzl","access_level":"open_access","file_name":"Extdata08_a_7.png","checksum":"0e2b768e4eb7200cf6fe28ba511a5ef6","success":1,"date_updated":"2023-08-03T13:04:53Z","date_created":"2023-08-03T13:04:53Z","relation":"main_file","file_id":"13684"},{"success":1,"checksum":"df4a01c62233ee8ed601c4b90d7780d0","date_created":"2023-08-03T13:04:57Z","date_updated":"2023-08-03T13:04:57Z","file_id":"13685","relation":"main_file","creator":"jdanzl","file_size":124540688,"content_type":"image/png","access_level":"open_access","file_name":"Extdata08_a_8.png"},{"content_type":"image/tiff","file_size":204861336,"creator":"jdanzl","file_name":"Exdata09_c_n2v.tif","access_level":"open_access","date_created":"2023-08-03T13:11:30Z","date_updated":"2023-08-03T13:11:30Z","checksum":"1f407c7915e2717a56fec258708e7b7a","success":1,"relation":"main_file","file_id":"13686"},{"file_size":252149,"content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata09_a_zoom.tif","access_level":"open_access","date_updated":"2023-08-03T13:11:26Z","date_created":"2023-08-03T13:11:26Z","checksum":"9089dc7c9914edf9750eebd65819708d","success":1,"relation":"main_file","file_id":"13687"},{"content_type":"image/tiff","file_size":11117705,"creator":"jdanzl","file_name":"Extdata09_a.tif","access_level":"open_access","date_created":"2023-08-03T13:11:26Z","date_updated":"2023-08-03T13:11:26Z","checksum":"62d295a62cb8dd36c268b28cf0251322","success":1,"relation":"main_file","file_id":"13688"},{"access_level":"open_access","file_name":"Extdata09_b_n2v.tif","file_size":256074660,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13689","checksum":"e874d8ee287f924c1076134b5b06bdb2","success":1,"date_created":"2023-08-03T13:11:31Z","date_updated":"2023-08-03T13:11:31Z"},{"creator":"jdanzl","file_size":127914854,"content_type":"image/tiff","access_level":"open_access","file_name":"Extdata10_n2v_CLAHE.tif","success":1,"checksum":"488068fff7b42bcf5728739e2a06d742","date_updated":"2023-08-03T13:12:53Z","date_created":"2023-08-03T13:12:53Z","file_id":"13690","relation":"main_file"},{"file_name":"Supplfig01_a_1.png","access_level":"open_access","content_type":"image/png","file_size":430878,"creator":"jdanzl","relation":"main_file","file_id":"13691","date_created":"2023-08-03T13:21:35Z","date_updated":"2023-08-03T13:21:35Z","checksum":"35351f5af21ce854d41577813a7bdb57","success":1},{"file_id":"13692","relation":"main_file","date_updated":"2023-08-03T13:21:35Z","date_created":"2023-08-03T13:21:35Z","success":1,"checksum":"36204e9e604206366ffc6f61ec91915b","file_name":"Supplfig01_a_2.png","access_level":"open_access","creator":"jdanzl","content_type":"image/png","file_size":438843},{"creator":"jdanzl","file_size":426713,"content_type":"image/png","access_level":"open_access","file_name":"Supplfig01_a_3.png","success":1,"checksum":"92ccc934c4603b29f4f7a96573e49c83","date_updated":"2023-08-03T13:21:35Z","date_created":"2023-08-03T13:21:35Z","file_id":"13693","relation":"main_file"},{"file_id":"13694","relation":"main_file","date_created":"2023-08-03T13:21:35Z","date_updated":"2023-08-03T13:21:35Z","success":1,"checksum":"e71faa54934bee8ac3e66ce692f542ab","file_name":"Supplfig01_a_4.tif","access_level":"open_access","creator":"jdanzl","file_size":723221,"content_type":"image/tiff"},{"date_created":"2023-08-03T13:21:35Z","date_updated":"2023-08-03T13:21:35Z","checksum":"95dc33563f48b650e5ac020bdfd10c48","success":1,"relation":"main_file","file_id":"13695","content_type":"image/tiff","file_size":723235,"creator":"jdanzl","file_name":"Supplfig01_a_5.tif","access_level":"open_access"},{"creator":"jdanzl","content_type":"image/tiff","file_size":1442129,"access_level":"open_access","file_name":"Supplfig01_b_1.tif","success":1,"checksum":"c62df0a9faf1c47af121d77eb1582edd","date_updated":"2023-08-03T13:21:35Z","date_created":"2023-08-03T13:21:35Z","file_id":"13696","relation":"main_file"},{"relation":"main_file","file_id":"13697","checksum":"8ae157c5a4a543da1193cb8ca12b1919","success":1,"date_created":"2023-08-03T13:21:35Z","date_updated":"2023-08-03T13:21:35Z","access_level":"open_access","file_name":"Supplfig01_b_2.tif","file_size":725407,"content_type":"image/tiff","creator":"jdanzl"},{"access_level":"open_access","file_name":"Supplfig01_c_1.tif","content_type":"image/tiff","file_size":721641,"creator":"jdanzl","relation":"main_file","file_id":"13698","checksum":"388370d954ce842781c0d62edfb448a7","success":1,"date_updated":"2023-08-03T13:21:35Z","date_created":"2023-08-03T13:21:35Z"},{"relation":"main_file","file_id":"13699","date_created":"2023-08-03T13:21:35Z","date_updated":"2023-08-03T13:21:35Z","checksum":"6cae58ba3c98bc8253e1c769e93657a7","success":1,"file_name":"Supplfig01_c_2.tif","access_level":"open_access","file_size":6003079,"content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","file_size":2003311,"access_level":"open_access","file_name":"Supplfig01_c_3.tif","success":1,"checksum":"85cf013232c72895aefc07f5411a765a","date_created":"2023-08-03T13:21:35Z","date_updated":"2023-08-03T13:21:35Z","file_id":"13700","relation":"main_file"},{"creator":"jdanzl","content_type":"image/tiff","file_size":723271,"access_level":"open_access","file_name":"Supplfig01_c_4.tif","success":1,"checksum":"dfb33b91066e3f34d3803f59a4105aba","date_created":"2023-08-03T13:21:35Z","date_updated":"2023-08-03T13:21:35Z","file_id":"13701","relation":"main_file"},{"creator":"jdanzl","file_size":721901,"content_type":"image/tiff","file_name":"Supplfig01_d_1.tif","access_level":"open_access","date_updated":"2023-08-03T13:21:35Z","date_created":"2023-08-03T13:21:35Z","success":1,"checksum":"8b899826fe48d7e7536b0b02a2645e87","file_id":"13702","relation":"main_file"},{"relation":"main_file","file_id":"13703","date_updated":"2023-08-03T13:21:35Z","date_created":"2023-08-03T13:21:35Z","checksum":"67529fa6e0f12c272275e823f6045f6d","success":1,"file_name":"Supplfig01_d_2.tif","access_level":"open_access","content_type":"image/tiff","file_size":721901,"creator":"jdanzl"},{"date_updated":"2023-08-03T13:21:35Z","date_created":"2023-08-03T13:21:35Z","checksum":"111a9231f63c775340d3356109715cb0","success":1,"relation":"main_file","file_id":"13704","content_type":"image/tiff","file_size":723223,"creator":"jdanzl","file_name":"Supplfig01_e_1.tif","access_level":"open_access"},{"date_updated":"2023-08-03T13:21:35Z","date_created":"2023-08-03T13:21:35Z","success":1,"checksum":"7685bf02408dcb095a00854ff22763ed","file_id":"13705","relation":"main_file","creator":"jdanzl","file_size":723223,"content_type":"image/tiff","file_name":"Supplfig01_e_2.tif","access_level":"open_access"},{"access_level":"open_access","file_name":"Supplfig02_b_01.tif","file_size":34821298,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13706","checksum":"455f5cb9aa8a87b91586a27aca122610","success":1,"date_updated":"2023-08-03T13:26:00Z","date_created":"2023-08-03T13:26:00Z"},{"access_level":"open_access","file_name":"Supplfig02_b_02.tif","content_type":"image/tiff","file_size":32131123,"creator":"jdanzl","relation":"main_file","file_id":"13707","checksum":"311545b39aaa2c72755fd8ae190803a5","success":1,"date_updated":"2023-08-03T13:26:00Z","date_created":"2023-08-03T13:26:00Z"},{"file_size":36872674,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig02_b_03.tif","access_level":"open_access","date_created":"2023-08-03T13:26:01Z","date_updated":"2023-08-03T13:26:01Z","checksum":"682deb61f57d9ca54716160ddd52a6cf","success":1,"relation":"main_file","file_id":"13708"},{"creator":"jdanzl","file_size":39964115,"content_type":"image/tiff","file_name":"Supplfig02_b_04.tif","access_level":"open_access","date_updated":"2023-08-03T13:26:01Z","date_created":"2023-08-03T13:26:01Z","success":1,"checksum":"4f9579c91b2a3f36ff6e66b592907973","file_id":"13709","relation":"main_file"},{"checksum":"7ae7053673b53dbed59ffb92618f969a","success":1,"date_updated":"2023-08-03T13:26:02Z","date_created":"2023-08-03T13:26:02Z","relation":"main_file","file_id":"13710","file_size":43053091,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig02_b_05.tif"},{"access_level":"open_access","file_name":"Supplfig02_b_06.tif","creator":"jdanzl","content_type":"image/tiff","file_size":46998147,"file_id":"13711","relation":"main_file","success":1,"checksum":"827b26d3d2b6ddc9bced9d6113023ced","date_updated":"2023-08-03T13:26:02Z","date_created":"2023-08-03T13:26:02Z"},{"file_name":"Supplfig02_b_07.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":45179219,"file_id":"13712","relation":"main_file","date_updated":"2023-08-03T13:26:03Z","date_created":"2023-08-03T13:26:03Z","success":1,"checksum":"bc2e7e8badb42f8e1facc8b7bc96fdda"},{"relation":"main_file","file_id":"13713","date_updated":"2023-08-03T13:26:03Z","date_created":"2023-08-03T13:26:03Z","checksum":"c2b78aa0f433928afa10a743c5fb73b8","success":1,"file_name":"Supplfig02_b_08.tif","access_level":"open_access","file_size":46978802,"content_type":"image/tiff","creator":"jdanzl"},{"file_name":"Supplfig02_b_09.tif","access_level":"open_access","creator":"jdanzl","file_size":48840915,"content_type":"image/tiff","file_id":"13714","relation":"main_file","date_created":"2023-08-03T13:26:04Z","date_updated":"2023-08-03T13:26:04Z","success":1,"checksum":"9cd1ab61cced2c0981e813aeae930855"},{"success":1,"checksum":"3086126338aee6ec4ebc1d3350551197","date_created":"2023-08-03T13:26:04Z","date_updated":"2023-08-03T13:26:04Z","file_id":"13715","relation":"main_file","creator":"jdanzl","file_size":45907059,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig02_b_10.tif"},{"relation":"main_file","file_id":"13716","checksum":"69f52c2b3bb5b5f9fef057860b3386fa","success":1,"date_created":"2023-08-03T13:26:05Z","date_updated":"2023-08-03T13:26:05Z","access_level":"open_access","file_name":"Supplfig02_b_11.tif","content_type":"image/tiff","file_size":32231538,"creator":"jdanzl"},{"date_created":"2023-08-03T13:26:05Z","date_updated":"2023-08-03T13:26:05Z","success":1,"checksum":"defdbeab1e672456bfeafdd1983e779e","file_id":"13717","relation":"main_file","creator":"jdanzl","file_size":35264963,"content_type":"image/tiff","file_name":"Supplfig02_b_12.tif","access_level":"open_access"},{"content_type":"image/png","file_size":63326,"creator":"jdanzl","file_name":"Supplfig03_a_std_xy.png","access_level":"open_access","date_updated":"2023-08-03T13:28:40Z","date_created":"2023-08-03T13:28:40Z","checksum":"02c566f781b9ad44f04ce0088a1fa27a","success":1,"relation":"main_file","file_id":"13718"},{"checksum":"c6c3630ee9a6ed5d207cb68e330da566","success":1,"date_created":"2023-08-03T13:28:40Z","date_updated":"2023-08-03T13:28:40Z","relation":"main_file","file_id":"13719","file_size":50211,"content_type":"image/png","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig03_a_std_xz.png"},{"date_created":"2023-08-03T13:28:41Z","date_updated":"2023-08-03T13:28:41Z","checksum":"0dcd4d19e83a7ff259d6aa04d746cd90","success":1,"relation":"main_file","file_id":"13720","file_size":52629,"content_type":"image/png","creator":"jdanzl","file_name":"Supplfig03_b_std_xy.png","access_level":"open_access"},{"content_type":"image/png","file_size":72245,"creator":"jdanzl","access_level":"open_access","file_name":"Supplfig03_b_std_xz.png","checksum":"4785de4a6dcb23b25a06b0da29df5bb5","success":1,"date_created":"2023-08-03T13:28:41Z","date_updated":"2023-08-03T13:28:41Z","relation":"main_file","file_id":"13721"},{"access_level":"open_access","file_name":"Supplfig03_model1.tif","creator":"jdanzl","file_size":98570368,"content_type":"image/tiff","file_id":"13722","relation":"main_file","success":1,"checksum":"19d0f198bd8a25073e45e7950d7a78c2","date_updated":"2023-08-03T13:28:45Z","date_created":"2023-08-03T13:28:45Z"},{"date_created":"2023-08-03T13:28:45Z","date_updated":"2023-08-03T13:28:45Z","success":1,"checksum":"51ff687df4a0867f39b69c2c7a4ee3ca","file_id":"13723","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":98570367,"file_name":"Supplfig03_model2.tif","access_level":"open_access"},{"access_level":"open_access","file_name":"Supplfig03_model3.tif","creator":"jdanzl","content_type":"image/tiff","file_size":98570365,"file_id":"13724","relation":"main_file","success":1,"checksum":"ec9d9a041ca85fe632c612baf981fcc5","date_updated":"2023-08-03T13:28:49Z","date_created":"2023-08-03T13:28:49Z"},{"creator":"jdanzl","content_type":"image/tiff","file_size":98570365,"access_level":"open_access","file_name":"Supplfig03_model4.tif","success":1,"checksum":"a988b15a9529094f1b5519a72871e98e","date_updated":"2023-08-03T13:28:49Z","date_created":"2023-08-03T13:28:49Z","file_id":"13725","relation":"main_file"},{"file_size":98570369,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig03_model5.tif","access_level":"open_access","date_created":"2023-08-03T13:28:52Z","date_updated":"2023-08-03T13:28:52Z","checksum":"4a4fe88fcfd87fcfe118a5a73d81d6b0","success":1,"relation":"main_file","file_id":"13726"},{"access_level":"open_access","file_name":"Supplfig04_a_n2v.tif","file_size":1036927887,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13727","checksum":"ce9b774ff5163ebfa4e3fb162f4cfffe","success":1,"date_updated":"2023-08-03T13:34:27Z","date_created":"2023-08-03T13:34:27Z"},{"creator":"jdanzl","content_type":"image/tiff","file_size":518519275,"access_level":"open_access","file_name":"Supplfig04_a.tif","success":1,"checksum":"21f1bb8d2a00c88bdd6febe6285acc2a","date_created":"2023-08-03T13:34:13Z","date_updated":"2023-08-03T13:34:13Z","file_id":"13728","relation":"main_file"},{"file_id":"13729","relation":"main_file","date_updated":"2023-08-03T13:34:22Z","date_created":"2023-08-03T13:34:22Z","success":1,"checksum":"503820214ab994c01b18a241f3b62434","file_name":"Supplfig04_b.tif","access_level":"open_access","creator":"jdanzl","file_size":158440069,"content_type":"image/tiff"},{"file_name":"Supplfig04_c_n2v.tif","access_level":"open_access","file_size":309407894,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13730","date_updated":"2023-08-03T13:34:37Z","date_created":"2023-08-03T13:34:37Z","checksum":"e0469102a87332b46904b4ba35b8fb2f","success":1},{"creator":"jdanzl","file_size":154728887,"content_type":"image/tiff","file_name":"Supplfig04_c.tif","access_level":"open_access","date_created":"2023-08-03T13:34:38Z","date_updated":"2023-08-03T13:34:38Z","success":1,"checksum":"c30a0d18c43045a8fbc5501a37061bd2","file_id":"13731","relation":"main_file"},{"checksum":"69c706710d642b30cda6951494702139","success":1,"date_created":"2023-08-03T13:34:43Z","date_updated":"2023-08-03T13:34:43Z","relation":"main_file","file_id":"13732","content_type":"image/tiff","file_size":106696749,"creator":"jdanzl","access_level":"open_access","file_name":"Supplfig04_d_n2v.tif"},{"access_level":"open_access","file_name":"Supplfig04_d.tif","creator":"jdanzl","file_size":53367343,"content_type":"image/tiff","file_id":"13733","relation":"main_file","success":1,"checksum":"7dbd2e881ebf049352913188fa10224a","date_updated":"2023-08-03T13:34:41Z","date_created":"2023-08-03T13:34:41Z"},{"file_name":"Supplfig04_e_n2v.tiff","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":127917734,"file_id":"13734","relation":"main_file","date_updated":"2023-08-03T13:34:46Z","date_created":"2023-08-03T13:34:46Z","success":1,"checksum":"fd0d1ec674dabbb499fab383b7eb4d58"},{"date_updated":"2023-08-03T13:34:46Z","date_created":"2023-08-03T13:34:46Z","success":1,"checksum":"86de309d9cd434afbe371da283d8adb6","file_id":"13735","relation":"main_file","creator":"jdanzl","file_size":63974204,"content_type":"image/tiff","file_name":"Supplfig04_e.tif","access_level":"open_access"},{"file_name":"Supplfig05_a_xy_1.png","access_level":"open_access","content_type":"image/png","file_size":8403,"creator":"jdanzl","relation":"main_file","file_id":"13736","date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","checksum":"ada99b2b5900cb198d1de3cc9e37ebd1","success":1},{"file_name":"Supplfig05_a_xy_2.png","access_level":"open_access","content_type":"image/png","file_size":10087,"creator":"jdanzl","relation":"main_file","file_id":"13737","date_updated":"2023-08-03T13:35:37Z","date_created":"2023-08-03T13:35:37Z","checksum":"f2e382925a972b98560614016ae3bc55","success":1},{"date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","checksum":"ef22b640f33d677add1a7b5153f95420","success":1,"relation":"main_file","file_id":"13738","content_type":"image/png","file_size":24561,"creator":"jdanzl","file_name":"Supplfig05_a_xy_3.png","access_level":"open_access"},{"access_level":"open_access","file_name":"Supplfig05_a_xy_4.png","file_size":8353,"content_type":"image/png","creator":"jdanzl","relation":"main_file","file_id":"13739","checksum":"b854cc4a5224b8a6f5d59c74f5740ccd","success":1,"date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z"},{"relation":"main_file","file_id":"13740","checksum":"446b0362c5ea6e2a5f0e1f8359422358","success":1,"date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","access_level":"open_access","file_name":"Supplfig05_a_xy_5.png","file_size":8337,"content_type":"image/png","creator":"jdanzl"},{"file_id":"13741","relation":"main_file","date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","success":1,"checksum":"5585f7b62fa09ed5a63090b679383601","file_name":"Supplfig05_a_xz_1.png","access_level":"open_access","creator":"jdanzl","file_size":7399,"content_type":"image/png"},{"access_level":"open_access","file_name":"Supplfig05_a_xz_2.png","creator":"jdanzl","content_type":"image/png","file_size":10887,"file_id":"13742","relation":"main_file","success":1,"checksum":"190d88bcacbd11d41f4a0e2bbd140862","date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z"},{"file_name":"Supplfig05_a_xz_3.png","access_level":"open_access","creator":"jdanzl","file_size":21654,"content_type":"image/png","file_id":"13743","relation":"main_file","date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","success":1,"checksum":"0c89010651862f21032a303e159b7a1c"},{"file_size":7248,"content_type":"image/png","creator":"jdanzl","file_name":"Supplfig05_a_xz_4.png","access_level":"open_access","date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","checksum":"94e61e553aa2280721acce7d407ff24a","success":1,"relation":"main_file","file_id":"13744"},{"checksum":"ef9157274686ff5d7056f5a519d413dd","success":1,"date_updated":"2023-08-03T13:35:37Z","date_created":"2023-08-03T13:35:37Z","relation":"main_file","file_id":"13745","file_size":7214,"content_type":"image/png","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig05_a_xz_5.png"},{"relation":"main_file","file_id":"13746","date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","checksum":"4b389473cbf769e6e34670dae2224af1","success":1,"file_name":"Supplfig05_b_xy_1.png","access_level":"open_access","file_size":8544,"content_type":"image/png","creator":"jdanzl"},{"creator":"jdanzl","file_size":11135,"content_type":"image/png","access_level":"open_access","file_name":"Supplfig05_b_xy_2.png","success":1,"checksum":"b05aa1e201758a5d55e35d719452fafc","date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","file_id":"13747","relation":"main_file"},{"file_name":"Supplfig05_b_xy_3.png","access_level":"open_access","creator":"jdanzl","content_type":"image/png","file_size":26762,"file_id":"13748","relation":"main_file","date_updated":"2023-08-03T13:35:37Z","date_created":"2023-08-03T13:35:37Z","success":1,"checksum":"890f3516930251a367066c049141d6bb"},{"relation":"main_file","file_id":"13749","checksum":"36345c1d582758ff463072fd04578c0c","success":1,"date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","access_level":"open_access","file_name":"Supplfig05_b_xy_4.png","content_type":"image/png","file_size":8438,"creator":"jdanzl"},{"checksum":"c9499ad8914c405a07fc4469b19e8987","success":1,"date_updated":"2023-08-03T13:35:37Z","date_created":"2023-08-03T13:35:37Z","relation":"main_file","file_id":"13750","file_size":8434,"content_type":"image/png","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig05_b_xy_5.png"},{"file_id":"13751","relation":"main_file","date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","success":1,"checksum":"7e7a7c900ce1fc3187ab648f207f9425","file_name":"Supplfig05_b_xz_1.png","access_level":"open_access","creator":"jdanzl","content_type":"image/png","file_size":8269},{"date_updated":"2023-08-03T13:35:37Z","date_created":"2023-08-03T13:35:37Z","success":1,"checksum":"b645d1e2422ef3f05344ccd3f955468d","file_id":"13752","relation":"main_file","creator":"jdanzl","file_size":12762,"content_type":"image/png","file_name":"Supplfig05_b_xz_2.png","access_level":"open_access"},{"access_level":"open_access","file_name":"Supplfig05_b_xz_3.png","creator":"jdanzl","file_size":24819,"content_type":"image/png","file_id":"13753","relation":"main_file","success":1,"checksum":"ef0b83b49cf47ca7d8ffe3bdc609fe38","date_updated":"2023-08-03T13:35:37Z","date_created":"2023-08-03T13:35:37Z"},{"date_created":"2023-08-03T13:35:37Z","date_updated":"2023-08-03T13:35:37Z","success":1,"checksum":"68719d4d464a2ffc198cb6ad070c4579","file_id":"13754","relation":"main_file","creator":"jdanzl","file_size":8126,"content_type":"image/png","file_name":"Supplfig05_b_xz_4.png","access_level":"open_access"},{"file_name":"Supplfig05_b_xz_5.png","access_level":"open_access","file_size":8114,"content_type":"image/png","creator":"jdanzl","relation":"main_file","file_id":"13755","date_created":"2023-08-03T13:35:38Z","date_updated":"2023-08-03T13:35:38Z","checksum":"18370f47177ef37a477c6fd7bda3fea0","success":1},{"access_level":"open_access","file_name":"Supplfig05_c_xy_1.png","creator":"jdanzl","file_size":8273,"content_type":"image/png","file_id":"13756","relation":"main_file","success":1,"checksum":"013d4bd9704ace490810b9ef21b12454","date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z"},{"date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","checksum":"bb431f827386e79c54452d6284f501b9","success":1,"relation":"main_file","file_id":"13757","content_type":"image/png","file_size":12639,"creator":"jdanzl","file_name":"Supplfig05_c_xy_2.png","access_level":"open_access"},{"success":1,"checksum":"4cd54f58c5afff88832a508655f913bb","date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","file_id":"13758","relation":"main_file","creator":"jdanzl","file_size":25288,"content_type":"image/png","access_level":"open_access","file_name":"Supplfig05_c_xy_3.png"},{"relation":"main_file","file_id":"13759","date_created":"2023-08-03T13:35:38Z","date_updated":"2023-08-03T13:35:38Z","checksum":"07f86f98f6a65eac8608cec8c567309e","success":1,"file_name":"Supplfig05_c_xz_1.png","access_level":"open_access","file_size":6786,"content_type":"image/png","creator":"jdanzl"},{"file_name":"Supplfig05_c_xz_2.png","access_level":"open_access","file_size":9781,"content_type":"image/png","creator":"jdanzl","relation":"main_file","file_id":"13760","date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","checksum":"492f699b71761214272b17432e3a54fa","success":1},{"checksum":"af617da5638db849bea6ff51a08227c6","success":1,"date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","relation":"main_file","file_id":"13761","file_size":20728,"content_type":"image/png","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig05_c_xz_3.png"},{"creator":"jdanzl","file_size":8428,"content_type":"image/png","file_name":"Supplfig05_d_xy_1.png","access_level":"open_access","date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","success":1,"checksum":"5e5d5694af4a22297046d697a01fa4d6","file_id":"13762","relation":"main_file"},{"date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","success":1,"checksum":"38bf7b75f3dedd0a3722faae05aa0233","file_id":"13763","relation":"main_file","creator":"jdanzl","content_type":"image/png","file_size":10813,"file_name":"Supplfig05_d_xy_2.png","access_level":"open_access"},{"date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","success":1,"checksum":"e4ea966553f158ec2cfbdc696780390e","file_id":"13764","relation":"main_file","creator":"jdanzl","content_type":"image/png","file_size":26552,"file_name":"Supplfig05_d_xy_3.png","access_level":"open_access"},{"content_type":"image/png","file_size":7876,"creator":"jdanzl","access_level":"open_access","file_name":"Supplfig05_d_xz_1.png","checksum":"356f424d9f5f608e9aea962bb63672f1","success":1,"date_created":"2023-08-03T13:35:38Z","date_updated":"2023-08-03T13:35:38Z","relation":"main_file","file_id":"13765"},{"creator":"jdanzl","content_type":"image/png","file_size":11634,"file_name":"Supplfig05_d_xz_2.png","access_level":"open_access","date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","success":1,"checksum":"05068476beee0f394e28b98209686394","file_id":"13766","relation":"main_file"},{"content_type":"image/png","file_size":25006,"creator":"jdanzl","access_level":"open_access","file_name":"Supplfig05_d_xz_3.png","checksum":"78b3a0d93df22383a384a2f3acc80401","success":1,"date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","relation":"main_file","file_id":"13767"},{"file_id":"13768","relation":"main_file","success":1,"checksum":"f5ee6a817f11a42c9db82ad6325f58b9","date_created":"2023-08-03T13:35:38Z","date_updated":"2023-08-03T13:35:38Z","access_level":"open_access","file_name":"Supplfig05_e_xy_1.png","creator":"jdanzl","file_size":7895,"content_type":"image/png"},{"date_created":"2023-08-03T13:35:38Z","date_updated":"2023-08-03T13:35:38Z","success":1,"checksum":"019ca89e892d6e19589609bc99eab331","file_id":"13769","relation":"main_file","creator":"jdanzl","content_type":"image/png","file_size":6180,"file_name":"Supplfig05_e_xy_2.png","access_level":"open_access"},{"success":1,"checksum":"0e1a5cbbd70dc245edaecaca70d974cb","date_created":"2023-08-03T13:35:38Z","date_updated":"2023-08-03T13:35:38Z","file_id":"13770","relation":"main_file","creator":"jdanzl","file_size":18326,"content_type":"image/png","access_level":"open_access","file_name":"Supplfig05_e_xy_3.png"},{"access_level":"open_access","file_name":"Supplfig05_e_xz_1.png","creator":"jdanzl","file_size":6295,"content_type":"image/png","file_id":"13771","relation":"main_file","success":1,"checksum":"4d4d64a5bb7b096aaf8130ece4512bc2","date_created":"2023-08-03T13:35:38Z","date_updated":"2023-08-03T13:35:38Z"},{"file_name":"Supplfig05_e_xz_2.png","access_level":"open_access","file_size":4335,"content_type":"image/png","creator":"jdanzl","relation":"main_file","file_id":"13772","date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","checksum":"607302aec5ec2a14a271dbffd3465261","success":1},{"file_name":"Supplfig05_e_xz_3.png","access_level":"open_access","file_size":23243,"content_type":"image/png","creator":"jdanzl","relation":"main_file","file_id":"13773","date_updated":"2023-08-03T13:35:38Z","date_created":"2023-08-03T13:35:38Z","checksum":"a673e17ead1fd2e776b9a5f560a289ab","success":1},{"relation":"main_file","file_id":"13774","date_created":"2023-08-03T13:46:13Z","date_updated":"2023-08-03T13:46:13Z","checksum":"4349e511fc86a8bddb790a7be7175c75","success":1,"file_name":"Supplfig06_a_1.tif","access_level":"open_access","file_size":4002153,"content_type":"image/tiff","creator":"jdanzl"},{"file_size":2252137,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig06_a_2.tif","checksum":"cd12977e4c1a472d80193be01f69a895","success":1,"date_updated":"2023-08-03T13:46:13Z","date_created":"2023-08-03T13:46:13Z","relation":"main_file","file_id":"13775"},{"file_name":"Supplfig06_a_3.tif","access_level":"open_access","content_type":"image/tiff","file_size":2252137,"creator":"jdanzl","relation":"main_file","file_id":"13776","date_updated":"2023-08-03T13:46:13Z","date_created":"2023-08-03T13:46:13Z","checksum":"575e32b8c25ce658e20f0c86a5f1b9b7","success":1},{"file_id":"13777","relation":"main_file","success":1,"checksum":"b06f775e0210e7a16cb4b58aed32118c","date_updated":"2023-08-03T13:46:13Z","date_created":"2023-08-03T13:46:13Z","access_level":"open_access","file_name":"Supplfig06_a_i.tif","creator":"jdanzl","file_size":78329,"content_type":"image/tiff"},{"checksum":"56854c1a05c721c36877fe7c7454b7d7","success":1,"date_created":"2023-08-03T13:46:13Z","date_updated":"2023-08-03T13:46:13Z","relation":"main_file","file_id":"13778","file_size":78329,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig06_a_ii.tif"},{"file_id":"13779","relation":"main_file","date_updated":"2023-08-03T13:46:13Z","date_created":"2023-08-03T13:46:13Z","success":1,"checksum":"9aff42ea5138931fedb5a00643ae7a22","file_name":"Supplfig06_a_iii.tif","access_level":"open_access","creator":"jdanzl","file_size":78329,"content_type":"image/tiff"},{"file_name":"Supplfig06_d_1.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":501149,"file_id":"13780","relation":"main_file","date_created":"2023-08-03T13:46:13Z","date_updated":"2023-08-03T13:46:13Z","success":1,"checksum":"9474b7d00d4dee46659afedef75dc095"},{"relation":"main_file","file_id":"13781","date_created":"2023-08-03T13:46:13Z","date_updated":"2023-08-03T13:46:13Z","checksum":"53df41dd968b04b4657cd2b91870d636","success":1,"file_name":"Supplfig06_d_2.tif","access_level":"open_access","content_type":"image/tiff","file_size":500153,"creator":"jdanzl"},{"file_size":500153,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig06_d_3.tif","checksum":"1a39b8310598dd25d97f848b682b8de0","success":1,"date_updated":"2023-08-03T13:46:14Z","date_created":"2023-08-03T13:46:14Z","relation":"main_file","file_id":"13782"},{"relation":"main_file","file_id":"13783","checksum":"6d9b496be6aecedbb2cb4cbf68f02872","success":1,"date_updated":"2023-08-03T13:46:14Z","date_created":"2023-08-03T13:46:14Z","access_level":"open_access","file_name":"Supplfig06_d_i.tif","file_size":78329,"content_type":"image/tiff","creator":"jdanzl"},{"content_type":"image/tiff","file_size":78329,"creator":"jdanzl","file_name":"Supplfig06_d_ii.tif","access_level":"open_access","date_created":"2023-08-03T13:46:14Z","date_updated":"2023-08-03T13:46:14Z","checksum":"bc034b1636c2db8b9d9e7bd131b9780a","success":1,"relation":"main_file","file_id":"13784"},{"date_created":"2023-08-03T13:46:14Z","date_updated":"2023-08-03T13:46:14Z","checksum":"54f6e55e30a2aeea6c82eb6dbd376240","success":1,"relation":"main_file","file_id":"13785","content_type":"image/tiff","file_size":78329,"creator":"jdanzl","file_name":"Supplfig06_d_iii.tif","access_level":"open_access"},{"file_size":960011,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig06_g_1.tif","checksum":"446da3cc16897277890f23f966c2bf52","success":1,"date_updated":"2023-08-03T13:46:14Z","date_created":"2023-08-03T13:46:14Z","relation":"main_file","file_id":"13786"},{"success":1,"checksum":"71192d0ec592a33195f2386a5f95aa86","date_created":"2023-08-03T13:46:14Z","date_updated":"2023-08-03T13:46:14Z","file_id":"13787","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":960011,"access_level":"open_access","file_name":"Supplfig06_g_2.tif"},{"creator":"jdanzl","file_size":960041,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig06_g_3.tif","success":1,"checksum":"246782dbe8ff525aea360efc72e65f07","date_created":"2023-08-03T13:46:14Z","date_updated":"2023-08-03T13:46:14Z","file_id":"13788","relation":"main_file"},{"content_type":"image/tiff","file_size":960041,"creator":"jdanzl","access_level":"open_access","file_name":"Supplfig06_g_4.tif","checksum":"d49b12fec5508aa8d72587757cccc0a4","success":1,"date_updated":"2023-08-03T13:46:14Z","date_created":"2023-08-03T13:46:14Z","relation":"main_file","file_id":"13789"},{"success":1,"checksum":"27b11b1d4fc784a49ffd7660624d00b6","date_updated":"2023-08-03T13:46:14Z","date_created":"2023-08-03T13:46:14Z","file_id":"13790","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":963241,"access_level":"open_access","file_name":"Supplfig06_g_5.tif"},{"date_updated":"2023-08-03T13:51:38Z","date_created":"2023-08-03T13:51:38Z","success":1,"checksum":"9ce944049c1b88fadbf43609385f9e2f","file_id":"13791","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":19286002,"file_name":"Suppl08_a_1.tiff","access_level":"open_access"},{"access_level":"open_access","file_name":"Suppl08_a_2.tiff","creator":"jdanzl","content_type":"image/tiff","file_size":19286002,"file_id":"13792","relation":"main_file","success":1,"checksum":"06265515f06688843a415362f7b2c485","date_created":"2023-08-03T13:51:38Z","date_updated":"2023-08-03T13:51:38Z"},{"file_name":"Suppl08_a_3.tif","access_level":"open_access","creator":"jdanzl","file_size":617552,"content_type":"image/tiff","file_id":"13793","relation":"main_file","date_created":"2023-08-03T13:51:38Z","date_updated":"2023-08-03T13:51:38Z","success":1,"checksum":"50f0969c6c2c1209ff776bec4861030a"},{"file_name":"Suppl08_a_4.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":617552,"file_id":"13794","relation":"main_file","date_updated":"2023-08-03T13:51:38Z","date_created":"2023-08-03T13:51:38Z","success":1,"checksum":"b90965d20133fea73c99050c32eb7695"},{"access_level":"open_access","file_name":"Suppl08_a_coCATS_N2V.tif","creator":"jdanzl","content_type":"image/tiff","file_size":19287339,"file_id":"13795","relation":"main_file","success":1,"checksum":"568a1e0efaa62527b51e66a7aabfd05c","date_updated":"2023-08-03T13:51:40Z","date_created":"2023-08-03T13:51:40Z"},{"file_id":"13796","relation":"main_file","success":1,"checksum":"06265515f06688843a415362f7b2c485","date_updated":"2023-08-03T13:51:40Z","date_created":"2023-08-03T13:51:40Z","access_level":"open_access","file_name":"Suppl08_c_1_4_predictedBASSOON.tiff","creator":"jdanzl","file_size":19286002,"content_type":"image/tiff"},{"checksum":"568a1e0efaa62527b51e66a7aabfd05c","success":1,"date_created":"2023-08-03T13:51:43Z","date_updated":"2023-08-03T13:51:43Z","relation":"main_file","file_id":"13797","content_type":"image/tiff","file_size":19287339,"creator":"jdanzl","access_level":"open_access","file_name":"Suppl08_c_1_coCATS.tif"},{"access_level":"open_access","file_name":"Suppl08_c_2_coCATS.tif","file_size":19287340,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13798","checksum":"feae08c38b1d9c540b697977cd7f2666","success":1,"date_updated":"2023-08-03T15:36:47Z","date_created":"2023-08-03T15:36:47Z"},{"file_name":"Suppl08_c_2-3_predictedBASSOON.tiff","access_level":"open_access","content_type":"image/tiff","file_size":19286002,"creator":"jdanzl","relation":"main_file","file_id":"13799","date_created":"2023-08-03T13:51:44Z","date_updated":"2023-08-03T13:51:44Z","checksum":"af2c1199fdcaecfec76189dae86cc12e","success":1},{"creator":"jdanzl","content_type":"application/octet-stream","file_size":156,"file_name":"Suppl08_c_3-6_roi.roi","access_level":"open_access","date_updated":"2023-08-03T13:51:43Z","date_created":"2023-08-03T13:51:43Z","success":1,"checksum":"1f92db47605959d0db01f612f7c9c034","file_id":"13800","relation":"main_file"},{"relation":"main_file","file_id":"13801","checksum":"4fc4f8803adcfacd53a41b91f4699dc5","success":1,"date_created":"2023-08-03T13:51:45Z","date_updated":"2023-08-03T13:51:45Z","access_level":"open_access","file_name":"Suppl08_e_1_3.tiff","content_type":"image/tiff","file_size":19286002,"creator":"jdanzl"},{"date_created":"2023-08-03T13:51:45Z","date_updated":"2023-08-03T13:51:45Z","success":1,"checksum":"459551aae114daa4f5ca5d06fcd6b203","file_id":"13802","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":19286002,"file_name":"Suppl08_e_1-2.tiff","access_level":"open_access"},{"content_type":"application/octet-stream","file_size":152,"creator":"jdanzl","file_name":"Suppl08_e_2-5_roi.roi","access_level":"open_access","date_updated":"2023-08-03T13:51:45Z","date_created":"2023-08-03T13:51:45Z","checksum":"032cbace7ec436f7b62478c0ffd35e5e","success":1,"relation":"main_file","file_id":"13803"},{"file_size":19286002,"content_type":"image/tiff","creator":"jdanzl","file_name":"Suppl08_e_4-5_coCATS.tiff","access_level":"open_access","date_updated":"2023-08-03T13:51:46Z","date_created":"2023-08-03T13:51:46Z","checksum":"7379168be281ef618b8cecd8efd31ed5","success":1,"relation":"main_file","file_id":"13804"},{"checksum":"5c4c092f608f4c10bb422c9e08b5955b","success":1,"date_updated":"2023-08-03T13:51:46Z","date_created":"2023-08-03T13:51:46Z","relation":"main_file","file_id":"13805","file_size":617552,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Suppl08_e_4.tif"},{"access_level":"open_access","file_name":"Suppl08_e_5.tif","creator":"jdanzl","file_size":617552,"content_type":"image/tiff","file_id":"13806","relation":"main_file","success":1,"checksum":"903e31be124f6e4ec1c2e7ab7db80e6b","date_updated":"2023-08-03T13:51:46Z","date_created":"2023-08-03T13:51:46Z"},{"access_level":"open_access","file_name":"Suppl08_e_6_8.tiff","creator":"jdanzl","content_type":"image/tiff","file_size":19286002,"file_id":"13807","relation":"main_file","success":1,"checksum":"173d956392d67b0a7e544629b51881e4","date_updated":"2023-08-03T13:51:48Z","date_created":"2023-08-03T13:51:48Z"},{"success":1,"checksum":"2a4cea867a253a6af7e9b0d2e100de14","date_updated":"2023-08-03T13:51:48Z","date_created":"2023-08-03T13:51:48Z","file_id":"13808","relation":"main_file","creator":"jdanzl","file_size":19286002,"content_type":"image/tiff","access_level":"open_access","file_name":"Suppl08_e_6-7.tiff"},{"checksum":"6b40f99fddfa26f0a9713000769741b7","success":1,"date_created":"2023-08-03T13:51:48Z","date_updated":"2023-08-03T13:51:48Z","relation":"main_file","file_id":"13809","file_size":174,"content_type":"application/octet-stream","creator":"jdanzl","access_level":"open_access","file_name":"Suppl08_e_7-10_roi.roi"},{"file_name":"Suppl08_e_9-10_coCATS.tiff","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":19286002,"file_id":"13810","relation":"main_file","date_created":"2023-08-03T13:51:49Z","date_updated":"2023-08-03T13:51:49Z","success":1,"checksum":"82f7013959df99c27366886de9b38ba2"},{"file_id":"13811","relation":"main_file","date_created":"2023-08-03T13:51:48Z","date_updated":"2023-08-03T13:51:48Z","success":1,"checksum":"50f0969c6c2c1209ff776bec4861030a","file_name":"Suppl08_e_9.tif","access_level":"open_access","creator":"jdanzl","file_size":617552,"content_type":"image/tiff"},{"date_updated":"2023-08-03T13:51:49Z","date_created":"2023-08-03T13:51:49Z","checksum":"569f599d7f2be49d4dcf7cb385f428c4","success":1,"relation":"main_file","file_id":"13812","file_size":617552,"content_type":"image/tiff","creator":"jdanzl","file_name":"Suppl08_e_10.tif","access_level":"open_access"},{"file_name":"Suppl08_g_1.tif","access_level":"open_access","content_type":"image/tiff","file_size":617552,"creator":"jdanzl","relation":"main_file","file_id":"13813","date_created":"2023-08-03T13:51:49Z","date_updated":"2023-08-03T13:51:49Z","checksum":"50f0969c6c2c1209ff776bec4861030a","success":1},{"file_name":"Suppl08_g_2.tif","access_level":"open_access","file_size":617552,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13814","date_updated":"2023-08-03T13:51:49Z","date_created":"2023-08-03T13:51:49Z","checksum":"62797fc860176197142a47e831eaf1f6","success":1},{"relation":"main_file","file_id":"13815","date_created":"2023-08-03T13:51:49Z","date_updated":"2023-08-03T13:51:49Z","checksum":"b90965d20133fea73c99050c32eb7695","success":1,"file_name":"Suppl08_g_3.tif","access_level":"open_access","content_type":"image/tiff","file_size":617552,"creator":"jdanzl"},{"relation":"main_file","file_id":"13816","date_created":"2023-08-03T13:51:49Z","date_updated":"2023-08-03T13:51:49Z","checksum":"a96dc9f38c6a1d3c959771a649a42748","success":1,"file_name":"Suppl08_i_add_roi.roi","access_level":"open_access","file_size":160,"content_type":"application/octet-stream","creator":"jdanzl"},{"relation":"main_file","file_id":"13817","checksum":"f2135207ebbc4bdc014763ba852a5cf0","success":1,"date_created":"2023-08-03T13:51:50Z","date_updated":"2023-08-03T13:51:50Z","access_level":"open_access","file_name":"Suppl08_i_shape_roi.roi","content_type":"application/octet-stream","file_size":160,"creator":"jdanzl"},{"file_name":"Suppl08_i_split_roi.roi","access_level":"open_access","file_size":158,"content_type":"application/octet-stream","creator":"jdanzl","relation":"main_file","file_id":"13818","date_created":"2023-08-03T13:51:50Z","date_updated":"2023-08-03T13:51:50Z","checksum":"f6f31fe2d87cf7208387f98ce1e37b04","success":1},{"access_level":"open_access","file_name":"Suppl08_i_1.tiff","file_size":19286002,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13819","checksum":"71590db0ad391e0c5baf03024981b1d9","success":1,"date_created":"2023-08-03T13:51:51Z","date_updated":"2023-08-03T13:51:51Z"},{"content_type":"image/tiff","file_size":19286002,"creator":"jdanzl","file_name":"Suppl08_i_2.tiff","access_level":"open_access","date_created":"2023-08-03T13:51:51Z","date_updated":"2023-08-03T13:51:51Z","checksum":"9ce944049c1b88fadbf43609385f9e2f","success":1,"relation":"main_file","file_id":"13820"},{"access_level":"open_access","file_name":"Suppl08_i_3.tiff","creator":"jdanzl","content_type":"image/tiff","file_size":19286002,"file_id":"13821","relation":"main_file","success":1,"checksum":"06265515f06688843a415362f7b2c485","date_updated":"2023-08-03T13:51:52Z","date_created":"2023-08-03T13:51:52Z"},{"relation":"main_file","file_id":"13822","checksum":"16c628c160fb81e40df0ad1eb134e92b","success":1,"date_updated":"2023-08-03T13:51:52Z","date_created":"2023-08-03T13:51:52Z","access_level":"open_access","file_name":"Suppl08_i_4.tif","file_size":19284766,"content_type":"image/tiff","creator":"jdanzl"},{"file_id":"13823","relation":"main_file","success":1,"checksum":"b06e62c3a9834a40002d7aad3eaacc3f","date_created":"2023-08-03T13:51:53Z","date_updated":"2023-08-03T13:51:53Z","access_level":"open_access","file_name":"Suppl08_i_5.tif","creator":"jdanzl","content_type":"image/tiff","file_size":9649818},{"content_type":"image/tiff","file_size":19284766,"creator":"jdanzl","file_name":"Suppl08_i_6.tif","access_level":"open_access","date_created":"2023-08-03T13:51:53Z","date_updated":"2023-08-03T13:51:53Z","checksum":"41ef98c7f01755b43cf6e77fff287732","success":1,"relation":"main_file","file_id":"13824"},{"file_size":19287340,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Suppl08_c_2_coCATS.tif","checksum":"feae08c38b1d9c540b697977cd7f2666","success":1,"date_updated":"2023-08-03T13:53:29Z","date_created":"2023-08-03T13:53:29Z","relation":"main_file","file_id":"13825"},{"file_size":179788642,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig09_b_1_n2v.tiff","access_level":"open_access","date_created":"2023-08-03T13:58:08Z","date_updated":"2023-08-03T13:58:08Z","checksum":"c01509a1c657fbbbfdbbd484c298b6e3","success":1,"relation":"main_file","file_id":"13826"},{"checksum":"64a7f270274ff3480b18d690087016e2","success":1,"date_created":"2023-08-03T13:58:20Z","date_updated":"2023-08-03T13:58:20Z","relation":"main_file","file_id":"13827","content_type":"image/tiff","file_size":742420240,"creator":"jdanzl","access_level":"open_access","file_name":"Supplfig09_b_2_n2v.tif"},{"file_name":"Supplfig09_b_3_n2v.tif","access_level":"open_access","content_type":"image/tiff","file_size":478900363,"creator":"jdanzl","relation":"main_file","file_id":"13828","date_created":"2023-08-03T13:58:20Z","date_updated":"2023-08-03T13:58:20Z","checksum":"b34a6beaaf13698f421df961e009efeb","success":1},{"file_name":"Supplfig09_b_4_n2v.tif","access_level":"open_access","content_type":"image/tiff","file_size":191873159,"creator":"jdanzl","relation":"main_file","file_id":"13829","date_created":"2023-08-03T13:58:25Z","date_updated":"2023-08-03T13:58:25Z","checksum":"ec04501f234b5e4fd91f9eac0e585395","success":1},{"file_name":"Supplfig11_i.tif","access_level":"open_access","file_size":55286,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13830","date_created":"2023-08-03T13:59:27Z","date_updated":"2023-08-03T13:59:27Z","checksum":"3a1b313298da35c97550abf0d245b4a7","success":1},{"file_name":"Supplfig11_ii.tif","access_level":"open_access","content_type":"image/tiff","file_size":55286,"creator":"jdanzl","relation":"main_file","file_id":"13831","date_created":"2023-08-03T13:59:27Z","date_updated":"2023-08-03T13:59:27Z","checksum":"9cf00c0252f0a2efe1994c83c224389a","success":1},{"file_size":55257,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig11_iii.tif","access_level":"open_access","date_created":"2023-08-03T13:59:27Z","date_updated":"2023-08-03T13:59:27Z","checksum":"9a6e83acdf6d70bb9a6e76fa7d41a372","success":1,"relation":"main_file","file_id":"13832"},{"file_name":"Supplfig11_n2v+prediction.tif","access_level":"open_access","file_size":309457551,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13833","date_updated":"2023-08-03T13:59:32Z","date_created":"2023-08-03T13:59:32Z","checksum":"709fdd13b7e090846a4d8edd2425f675","success":1},{"file_name":"Supplfig12_a_01_n2v.tif","access_level":"open_access","creator":"jdanzl","file_size":119323629,"content_type":"image/tiff","file_id":"13834","relation":"main_file","date_updated":"2023-08-03T14:14:32Z","date_created":"2023-08-03T14:14:32Z","success":1,"checksum":"e60ea3e6bb1bf380460d0d8ccdbd1f07"},{"relation":"main_file","file_id":"13835","date_created":"2023-08-03T14:14:29Z","date_updated":"2023-08-03T14:14:29Z","checksum":"2a4e1771e2f06383894361ba2a8155b4","success":1,"file_name":"Supplfig12_a_01.tif","access_level":"open_access","file_size":59680727,"content_type":"image/tiff","creator":"jdanzl"},{"file_size":106696749,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig12_a_02_n2v.tif","checksum":"993c434069b9d1bcb09bfe70628f5bbe","success":1,"date_created":"2023-08-03T14:14:34Z","date_updated":"2023-08-03T14:14:34Z","relation":"main_file","file_id":"13836"},{"date_updated":"2023-08-03T14:14:34Z","date_created":"2023-08-03T14:14:34Z","checksum":"e3b6410d68e42004ef3857967707aeb3","success":1,"relation":"main_file","file_id":"13837","file_size":53367287,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig12_a_02.tif","access_level":"open_access"},{"file_name":"Supplfig12_a_03_n2v.tif","access_level":"open_access","creator":"jdanzl","file_size":149937979,"content_type":"image/tiff","file_id":"13838","relation":"main_file","date_created":"2023-08-03T14:14:41Z","date_updated":"2023-08-03T14:14:41Z","success":1,"checksum":"0156e99806fe1539d5f48ea16c78e8c8"},{"date_updated":"2023-08-03T14:14:39Z","date_created":"2023-08-03T14:14:39Z","checksum":"6cc1533a221526a072a0b09fb44bcf50","success":1,"relation":"main_file","file_id":"13839","file_size":74987927,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig12_a_03.tif","access_level":"open_access"},{"success":1,"checksum":"f134834bd0d5e8c22e5a35aadf592007","date_updated":"2023-08-03T14:14:53Z","date_created":"2023-08-03T14:14:53Z","file_id":"13840","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":305581862,"access_level":"open_access","file_name":"Supplfig12_a_04_n2v.tif"},{"file_name":"Supplfig12_a_04.tif","access_level":"open_access","creator":"jdanzl","file_size":152821840,"content_type":"image/tiff","file_id":"13841","relation":"main_file","date_updated":"2023-08-03T14:14:50Z","date_created":"2023-08-03T14:14:50Z","success":1,"checksum":"212ec76c02552fa1eef91e7bca9f07f7"},{"file_size":48082029,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig12_a_05_n2v.tif","checksum":"7d99807d5b4313b04b4b5e0fbc08ba78","success":1,"date_created":"2023-08-03T14:14:52Z","date_updated":"2023-08-03T14:14:52Z","relation":"main_file","file_id":"13842"},{"access_level":"open_access","file_name":"Supplfig12_a_05.tif","creator":"jdanzl","content_type":"image/tiff","file_size":24059983,"file_id":"13843","relation":"main_file","success":1,"checksum":"a6d4024a8c6fa073a532cc0c78323df7","date_updated":"2023-08-03T14:14:53Z","date_created":"2023-08-03T14:14:53Z"},{"file_name":"Supplfig12_a_06_n2v.tif","access_level":"open_access","creator":"jdanzl","file_size":305581860,"content_type":"image/tiff","file_id":"13844","relation":"main_file","date_created":"2023-08-03T14:15:09Z","date_updated":"2023-08-03T14:15:09Z","success":1,"checksum":"1c42ad5423636478fe57495a3b506746"},{"relation":"main_file","file_id":"13845","checksum":"821f1d31c6dbe68eb2513fb7520b5272","success":1,"date_updated":"2023-08-03T14:15:04Z","date_created":"2023-08-03T14:15:04Z","access_level":"open_access","file_name":"Supplfig12_a_06.tif","content_type":"image/tiff","file_size":152821840,"creator":"jdanzl"},{"file_id":"13846","relation":"main_file","date_created":"2023-08-03T14:15:08Z","date_updated":"2023-08-03T14:15:08Z","success":1,"checksum":"86a4dc1e4948c4bc9c71e6d55b4fd104","file_name":"Supplfig12_a_07_n2v.tif","access_level":"open_access","creator":"jdanzl","file_size":66855793,"content_type":"image/tiff"},{"date_updated":"2023-08-03T14:15:09Z","date_created":"2023-08-03T14:15:09Z","success":1,"checksum":"fd5d45b1135800124fec635c4667dafe","file_id":"13847","relation":"main_file","creator":"jdanzl","file_size":33446807,"content_type":"image/tiff","file_name":"Supplfig12_a_07.tif","access_level":"open_access"},{"file_id":"13848","relation":"main_file","date_updated":"2023-08-03T14:15:19Z","date_created":"2023-08-03T14:15:19Z","success":1,"checksum":"5683760cc119a09f0b7de8fea98cda9c","file_name":"Supplfig12_a_08_n2v.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":231476260},{"creator":"jdanzl","content_type":"image/tiff","file_size":115769040,"file_name":"Supplfig12_a_08.tif","access_level":"open_access","date_updated":"2023-08-03T14:15:15Z","date_created":"2023-08-03T14:15:15Z","success":1,"checksum":"0c336d554bcd8524f11bd25c2188b56f","file_id":"13849","relation":"main_file"},{"content_type":"image/tiff","file_size":305581869,"creator":"jdanzl","file_name":"Supplfig12_a_09_n2v.tif","access_level":"open_access","date_created":"2023-08-03T14:15:31Z","date_updated":"2023-08-03T14:15:31Z","checksum":"fcaea6c95c363bd864a524fca8ed8e9b","success":1,"relation":"main_file","file_id":"13850"},{"content_type":"image/tiff","file_size":152821840,"creator":"jdanzl","access_level":"open_access","file_name":"Supplfig12_a_09.tif","checksum":"f401f1875f072c345d106a1e419f9d13","success":1,"date_updated":"2023-08-03T14:15:28Z","date_created":"2023-08-03T14:15:28Z","relation":"main_file","file_id":"13851"},{"checksum":"7ad7d5b1ac81da1ec3d5003d00ee870a","success":1,"date_updated":"2023-08-03T14:15:42Z","date_created":"2023-08-03T14:15:42Z","relation":"main_file","file_id":"13852","file_size":266386255,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig12_a_10_n2v.tif"},{"file_name":"Supplfig12_a_10.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":133239040,"file_id":"13853","relation":"main_file","date_created":"2023-08-03T14:15:40Z","date_updated":"2023-08-03T14:15:40Z","success":1,"checksum":"77b93d082debd5248348c0a1e886eab9"},{"file_name":"Supplfig12_a_11_n2v.tif","access_level":"open_access","creator":"jdanzl","file_size":191866269,"content_type":"image/tiff","file_id":"13854","relation":"main_file","date_created":"2023-08-03T14:15:49Z","date_updated":"2023-08-03T14:15:49Z","success":1,"checksum":"5ece1d503f05133d07d5b867f666d7a8"},{"file_id":"13855","relation":"main_file","date_updated":"2023-08-03T14:15:48Z","date_created":"2023-08-03T14:15:48Z","success":1,"checksum":"a72ee7ab433097752ff00a63421d4b61","file_name":"Supplfig12_a_11.tif","access_level":"open_access","creator":"jdanzl","file_size":95955040,"content_type":"image/tiff"},{"file_id":"13856","relation":"main_file","date_updated":"2023-08-03T14:16:49Z","date_created":"2023-08-03T14:16:49Z","success":1,"checksum":"c9c6e4692e0e7f305a43058b201b50d3","file_name":"Supplfig13_i.tif","access_level":"open_access","creator":"jdanzl","file_size":55281,"content_type":"image/tiff"},{"creator":"jdanzl","content_type":"image/tiff","file_size":55193,"file_name":"Supplfig13_ii.tif","access_level":"open_access","date_updated":"2023-08-03T14:16:49Z","date_created":"2023-08-03T14:16:49Z","success":1,"checksum":"345d11dbd70123d9d2723b547cbfc4fc","file_id":"13857","relation":"main_file"},{"access_level":"open_access","file_name":"Supplfig13_n2v+predict.tif","creator":"jdanzl","content_type":"image/tiff","file_size":539037,"file_id":"13858","relation":"main_file","success":1,"checksum":"b4c74c08077cfd3e11bfd6ea9ee1c5b2","date_created":"2023-08-03T14:16:49Z","date_updated":"2023-08-03T14:16:49Z"},{"creator":"jdanzl","file_size":854898609,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig14_a_n2v.tif","success":1,"checksum":"15f5b44257d075f9e45811304dfd5f85","date_updated":"2023-08-03T14:18:10Z","date_created":"2023-08-03T14:18:10Z","file_id":"13859","relation":"main_file"},{"access_level":"open_access","file_name":"Supplfig15_a_1.png","content_type":"image/png","file_size":7514502,"creator":"jdanzl","relation":"main_file","file_id":"13860","checksum":"a9ca03e3e5696988d06fc6ab1c103440","success":1,"date_updated":"2023-08-03T14:19:58Z","date_created":"2023-08-03T14:19:58Z"},{"access_level":"open_access","file_name":"Supplfig15_a_2.png","creator":"jdanzl","file_size":1784041,"content_type":"image/png","file_id":"13861","relation":"main_file","success":1,"checksum":"4be3451e203a0fc5d9eb14279ff68a7a","date_updated":"2023-08-03T14:19:58Z","date_created":"2023-08-03T14:19:58Z"},{"file_size":6030878,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig15_b_1.tif","checksum":"43068c31eecddb798dbf2f51bda9b6c9","success":1,"date_updated":"2023-08-03T14:19:58Z","date_created":"2023-08-03T14:19:58Z","relation":"main_file","file_id":"13862"},{"relation":"main_file","file_id":"13863","checksum":"ded4c0797f1a9152dc3128f1bb8c32a5","success":1,"date_created":"2023-08-03T14:19:58Z","date_updated":"2023-08-03T14:19:58Z","access_level":"open_access","file_name":"Supplfig15_b_2.tif","file_size":723293,"content_type":"image/tiff","creator":"jdanzl"},{"file_name":"Supplfig15_c_i.tif","access_level":"open_access","content_type":"image/tiff","file_size":723409,"creator":"jdanzl","relation":"main_file","file_id":"13864","date_created":"2023-08-03T14:19:58Z","date_updated":"2023-08-03T14:19:58Z","checksum":"705394732d94bd8463479aee83c2d36e","success":1},{"success":1,"checksum":"e08efe220bec6a93d583cab17754dd19","date_created":"2023-08-03T14:19:58Z","date_updated":"2023-08-03T14:19:58Z","file_id":"13865","relation":"main_file","creator":"jdanzl","file_size":723409,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig15_c_ii.tif"},{"date_updated":"2023-08-03T14:19:59Z","date_created":"2023-08-03T14:19:59Z","checksum":"0e08e5d965815240b0a8195e0f899aab","success":1,"relation":"main_file","file_id":"13866","file_size":31572914,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig15_c.tif","access_level":"open_access"},{"file_size":6030752,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig15_d_1.tif","checksum":"594696a77d4a9be512046be5e2d602f0","success":1,"date_updated":"2023-08-03T14:19:59Z","date_created":"2023-08-03T14:19:59Z","relation":"main_file","file_id":"13867"},{"file_name":"Supplfig15_d_2.tif","access_level":"open_access","file_size":8005495,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13868","date_updated":"2023-08-03T14:19:59Z","date_created":"2023-08-03T14:19:59Z","checksum":"ffe6733eb77858008c4ca07b5f8c432b","success":1},{"content_type":"image/tiff","file_size":11117709,"creator":"jdanzl","access_level":"open_access","file_name":"Supplfig16_a.tif","checksum":"663bf013cf1e5019e1e7ddd2d12fa492","success":1,"date_updated":"2023-08-03T14:20:56Z","date_created":"2023-08-03T14:20:56Z","relation":"main_file","file_id":"13869"},{"date_updated":"2023-08-03T14:20:56Z","date_created":"2023-08-03T14:20:56Z","success":1,"checksum":"80aee233dc2a1f26ed8cc323270cf3a9","file_id":"13870","relation":"main_file","creator":"jdanzl","file_size":113709,"content_type":"image/tiff","file_name":"Supplfig16_b.tif","access_level":"open_access"},{"file_size":113709,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig16_c.tif","checksum":"3b408e9345df2f16a942ad4ea61739b7","success":1,"date_updated":"2023-08-03T14:20:56Z","date_created":"2023-08-03T14:20:56Z","relation":"main_file","file_id":"13871"},{"file_name":"Supplfig17_a_1.tif","access_level":"open_access","creator":"jdanzl","file_size":6003131,"content_type":"image/tiff","file_id":"13872","relation":"main_file","date_updated":"2023-08-03T14:25:47Z","date_created":"2023-08-03T14:25:47Z","success":1,"checksum":"fbf688ef1f907149f92e9e936d02df72"},{"date_updated":"2023-08-03T14:25:47Z","date_created":"2023-08-03T14:25:47Z","checksum":"9a56470f251f14b43903036fab338f76","success":1,"relation":"main_file","file_id":"13873","file_size":8004085,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig17_a_2.tif","access_level":"open_access"},{"file_size":6003123,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig17_a_3.tif","access_level":"open_access","date_updated":"2023-08-03T14:25:47Z","date_created":"2023-08-03T14:25:47Z","checksum":"4578c020ba5d0a6ffe2a8a1c22e52c75","success":1,"relation":"main_file","file_id":"13874"},{"creator":"jdanzl","content_type":"image/tiff","file_size":6003131,"file_name":"Supplfig17_a_4.tif","access_level":"open_access","date_updated":"2023-08-03T14:25:47Z","date_created":"2023-08-03T14:25:47Z","success":1,"checksum":"a681d32f515da3bff8e803946f1d8b50","file_id":"13875","relation":"main_file"},{"success":1,"checksum":"b89d366c39e25d17dc5d8ceee9145c8b","date_updated":"2023-08-03T14:25:47Z","date_created":"2023-08-03T14:25:47Z","file_id":"13876","relation":"main_file","creator":"jdanzl","file_size":6003131,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig17_a_5.tif"},{"file_id":"13877","relation":"main_file","success":1,"checksum":"0b9d60f0aba79525a97c122d972f0b22","date_created":"2023-08-03T14:25:47Z","date_updated":"2023-08-03T14:25:47Z","access_level":"open_access","file_name":"Supplfig17_a_6.tif","creator":"jdanzl","content_type":"image/tiff","file_size":6003079},{"file_id":"13878","relation":"main_file","success":1,"checksum":"169aad3cea521cc2a0ce4c3e41473e0c","date_updated":"2023-08-03T14:25:47Z","date_created":"2023-08-03T14:25:47Z","access_level":"open_access","file_name":"Supplfig17_a_7.tif","creator":"jdanzl","content_type":"image/tiff","file_size":6003115},{"date_updated":"2023-08-03T14:25:47Z","date_created":"2023-08-03T14:25:47Z","checksum":"39a44d77d9047bfa10da8d9c9bd7b391","success":1,"relation":"main_file","file_id":"13879","file_size":4002129,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig17_a_8.tif","access_level":"open_access"},{"file_name":"Supplfig17_b.tif","access_level":"open_access","file_size":8214335,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13880","date_created":"2023-08-03T14:25:48Z","date_updated":"2023-08-03T14:25:48Z","checksum":"f4ecb2bd44f2cea3d71587670344ac40","success":1},{"relation":"main_file","file_id":"13881","checksum":"44a20d2621680c90227416bf32b5a566","success":1,"date_updated":"2023-08-03T14:27:06Z","date_created":"2023-08-03T14:27:06Z","access_level":"open_access","file_name":"Supplfig18_a.tif","content_type":"image/tiff","file_size":7471479,"creator":"jdanzl"},{"file_size":290625,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig18_b.tif","checksum":"5a5f7b616785f9213064e8c77f9d38c4","success":1,"date_updated":"2023-08-03T14:27:06Z","date_created":"2023-08-03T14:27:06Z","relation":"main_file","file_id":"13882"},{"file_id":"13883","relation":"main_file","success":1,"checksum":"8f621722a636e808a03d85f9597ad1a2","date_created":"2023-08-03T14:27:07Z","date_updated":"2023-08-03T14:27:07Z","access_level":"open_access","file_name":"Supplfig18_c.tif","creator":"jdanzl","content_type":"image/tiff","file_size":411767},{"success":1,"checksum":"3c8356f9170fe345e2ef725cd7d1b167","date_created":"2023-08-03T14:27:07Z","date_updated":"2023-08-03T14:27:07Z","file_id":"13884","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":11117685,"access_level":"open_access","file_name":"Supplfig18_d.tif"},{"success":1,"checksum":"5c8443e97724b54f359f7355483b890e","date_updated":"2023-08-03T14:27:10Z","date_created":"2023-08-03T14:27:10Z","file_id":"13885","relation":"main_file","creator":"jdanzl","file_size":259509773,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig18_e_n2v.tif"},{"relation":"main_file","file_id":"13886","date_updated":"2023-08-03T14:29:25Z","date_created":"2023-08-03T14:29:25Z","checksum":"413e3105c1bcd2ac2c569fa2b06253f2","success":1,"file_name":"Supplfig19_n2v.tif","access_level":"open_access","content_type":"image/tiff","file_size":1430939360,"creator":"jdanzl"},{"success":1,"checksum":"a864dc5b54f469c723e86286d538bb52","date_updated":"2023-08-03T14:29:16Z","date_created":"2023-08-03T14:29:16Z","file_id":"13887","relation":"main_file","creator":"jdanzl","file_size":715524468,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig19.tif"},{"file_id":"13888","relation":"main_file","success":1,"checksum":"8a78ce49446d207c0bf31196b788fa93","date_updated":"2023-08-03T14:32:54Z","date_created":"2023-08-03T14:32:54Z","access_level":"open_access","file_name":"Supplfig20_a_left.tif","creator":"jdanzl","file_size":8004743,"content_type":"image/tiff"},{"file_name":"Supplfig20_a_right.tif","access_level":"open_access","file_size":5561081,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13889","date_created":"2023-08-03T14:32:54Z","date_updated":"2023-08-03T14:32:54Z","checksum":"48fa3abfd16a00c7a3757d6452a482ec","success":1},{"file_name":"Supplfig20_b_1.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":8456797,"file_id":"13890","relation":"main_file","date_updated":"2023-08-03T14:32:54Z","date_created":"2023-08-03T14:32:54Z","success":1,"checksum":"85d82cd94dd3202f04b5987bc4466573"},{"file_name":"Supplfig20_b_2.tif","access_level":"open_access","content_type":"image/tiff","file_size":8456795,"creator":"jdanzl","relation":"main_file","file_id":"13891","date_updated":"2023-08-03T14:32:54Z","date_created":"2023-08-03T14:32:54Z","checksum":"e16f04d5d4d40f6ec33456d0cf02e1eb","success":1},{"file_id":"13892","relation":"main_file","success":1,"checksum":"ff190edb269c28b25d27bffaeb7d25cf","date_created":"2023-08-03T14:32:54Z","date_updated":"2023-08-03T14:32:54Z","access_level":"open_access","file_name":"Supplfig20_b_3.tif","creator":"jdanzl","content_type":"image/tiff","file_size":8456796},{"date_updated":"2023-08-03T14:32:54Z","date_created":"2023-08-03T14:32:54Z","checksum":"deb10b81bae62d9e20e681411fb75fcc","success":1,"relation":"main_file","file_id":"13893","content_type":"image/tiff","file_size":8538887,"creator":"jdanzl","file_name":"Supplfig20_b_4.tif","access_level":"open_access"},{"checksum":"5c64bb94e76030d7a9d8624bac4b0f57","success":1,"date_updated":"2023-08-03T14:32:54Z","date_created":"2023-08-03T14:32:54Z","relation":"main_file","file_id":"13894","file_size":8538851,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig20_b_5.tif"},{"relation":"main_file","file_id":"13895","checksum":"d6c20232c4bd36e213b4c456c5009644","success":1,"date_created":"2023-08-03T14:32:54Z","date_updated":"2023-08-03T14:32:54Z","access_level":"open_access","file_name":"Supplfig20_c_1.tif","file_size":11077737,"content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","file_size":11077737,"access_level":"open_access","file_name":"Supplfig20_c_2.tif","success":1,"checksum":"49c3396b8960af3f6fd8f6b078971d51","date_updated":"2023-08-03T14:32:54Z","date_created":"2023-08-03T14:32:54Z","file_id":"13896","relation":"main_file"},{"file_name":"Supplfig20_c_3.tif","access_level":"open_access","creator":"jdanzl","file_size":11077737,"content_type":"image/tiff","file_id":"13897","relation":"main_file","date_updated":"2023-08-03T14:32:55Z","date_created":"2023-08-03T14:32:55Z","success":1,"checksum":"73471528f691fb337839b84c31014da4"},{"file_id":"13898","relation":"main_file","success":1,"checksum":"47a0fd1a36d7e7907ce18007bdaaa145","date_created":"2023-08-03T14:32:55Z","date_updated":"2023-08-03T14:32:55Z","access_level":"open_access","file_name":"Supplfig20_c_4.tif","creator":"jdanzl","file_size":11077737,"content_type":"image/tiff"},{"creator":"jdanzl","file_size":11077737,"content_type":"image/tiff","file_name":"Supplfig20_c_5.tif","access_level":"open_access","date_updated":"2023-08-03T14:32:55Z","date_created":"2023-08-03T14:32:55Z","success":1,"checksum":"0c74b07f13fb08a91e61669368f38c9a","file_id":"13899","relation":"main_file"},{"file_id":"13900","relation":"main_file","date_created":"2023-08-03T14:32:55Z","date_updated":"2023-08-03T14:32:55Z","success":1,"checksum":"fe3b76446ec8bd0d5cdba94a128a84b8","file_name":"Supplfig20_d_1.tif","access_level":"open_access","creator":"jdanzl","file_size":4002133,"content_type":"image/tiff"},{"file_id":"13901","relation":"main_file","date_created":"2023-08-03T14:32:55Z","date_updated":"2023-08-03T14:32:55Z","success":1,"checksum":"0570bab9a471af3d0d1d8bf9a3cb17e7","file_name":"Supplfig20_d_2.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":4002133},{"relation":"main_file","file_id":"13902","date_created":"2023-08-03T14:32:55Z","date_updated":"2023-08-03T14:32:55Z","checksum":"59f330990b5c95f98a768761a009940c","success":1,"file_name":"Supplfig20_d_3.tif","access_level":"open_access","content_type":"image/tiff","file_size":4002199,"creator":"jdanzl"},{"file_id":"13903","relation":"main_file","success":1,"checksum":"f0da06f2a278dff4bbd1aeace11700bf","date_created":"2023-08-03T14:32:55Z","date_updated":"2023-08-03T14:32:55Z","access_level":"open_access","file_name":"Supplfig20_d_4.tif","creator":"jdanzl","content_type":"image/tiff","file_size":4002133},{"relation":"main_file","file_id":"13904","checksum":"e61d49dcc0952af3623cc26a78cc6495","success":1,"date_updated":"2023-08-03T14:32:55Z","date_created":"2023-08-03T14:32:55Z","access_level":"open_access","file_name":"Supplfig20_d_5.tif","file_size":4002133,"content_type":"image/tiff","creator":"jdanzl"},{"access_level":"open_access","file_name":"Supplfig22_b_1.tif","file_size":12627576,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13905","checksum":"bcf8294487abaf1a75033f388fba06ec","success":1,"date_updated":"2023-08-03T14:42:01Z","date_created":"2023-08-03T14:42:01Z"},{"file_id":"13906","relation":"main_file","success":1,"checksum":"14097feb4a73e7bb1e1694cef0115cab","date_updated":"2023-08-03T14:42:01Z","date_created":"2023-08-03T14:42:01Z","access_level":"open_access","file_name":"Supplfig22_b_2.tif","creator":"jdanzl","file_size":8543366,"content_type":"image/tiff"},{"checksum":"7e74b8cff2ada56d47d135a6a9e71e76","success":1,"date_updated":"2023-08-03T14:42:04Z","date_created":"2023-08-03T14:42:04Z","relation":"main_file","file_id":"13907","file_size":8394010,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig22_b_3.tif"},{"date_updated":"2023-08-03T15:35:39Z","date_created":"2023-08-03T15:35:39Z","checksum":"f6e6e0f0e3854fedd201ab8ff08a7651","success":1,"relation":"main_file","file_id":"13908","file_size":8682992,"content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig22_b_4.tif","access_level":"open_access"},{"date_updated":"2023-08-03T14:42:04Z","date_created":"2023-08-03T14:42:04Z","success":1,"checksum":"6ddeb84249768b46b9c2072bf85bdc8b","file_id":"13909","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":8503466,"file_name":"Supplfig22_b_5.tif","access_level":"open_access"},{"access_level":"open_access","file_name":"Supplfig22_b_6.tif","creator":"jdanzl","content_type":"image/tiff","file_size":8543342,"file_id":"13910","relation":"main_file","success":1,"checksum":"40648b8a4ef375b8352f11a6772ea1a6","date_created":"2023-08-03T14:42:04Z","date_updated":"2023-08-03T14:42:04Z"},{"relation":"main_file","file_id":"13911","date_created":"2023-08-03T14:42:06Z","date_updated":"2023-08-03T14:42:06Z","checksum":"78f601de4d88f6ccb97861eaf4a995f8","success":1,"file_name":"Supplfig22_b_7.tif","access_level":"open_access","content_type":"image/tiff","file_size":33588990,"creator":"jdanzl"},{"file_name":"Supplfig22_c.tif","access_level":"open_access","content_type":"image/tiff","file_size":1453171518,"creator":"jdanzl","relation":"main_file","file_id":"13912","date_created":"2023-08-03T14:42:41Z","date_updated":"2023-08-03T14:42:41Z","checksum":"2873c488b7c8ad4d111726025086e9ea","success":1},{"file_size":1325919782,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig23_a.tif","checksum":"c1d0e5e61ac0d0ddf08704769f80ac78","success":1,"date_updated":"2023-08-03T14:47:43Z","date_created":"2023-08-03T14:47:43Z","relation":"main_file","file_id":"13913"},{"access_level":"open_access","file_name":"Supplfig23_b.tif","creator":"jdanzl","file_size":31366288,"content_type":"image/tiff","file_id":"13914","relation":"main_file","success":1,"checksum":"f97632984b6a73e06cc0c8c947cdcc71","date_created":"2023-08-03T14:47:25Z","date_updated":"2023-08-03T14:47:25Z"},{"file_name":"Supplfig23_c.tif","access_level":"open_access","file_size":31366288,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13915","date_created":"2023-08-03T14:47:26Z","date_updated":"2023-08-03T14:47:26Z","checksum":"16f4c17835a79d7c06e2a97b1603baf1","success":1},{"access_level":"open_access","file_name":"Supplfig23_d.tif","content_type":"image/tiff","file_size":31366428,"creator":"jdanzl","relation":"main_file","file_id":"13916","checksum":"00f35ca77165642ca9b3bc3608bd04c1","success":1,"date_updated":"2023-08-03T14:47:27Z","date_created":"2023-08-03T14:47:27Z"},{"file_name":"Supplfig23_e.tif","access_level":"open_access","file_size":31500832,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13917","date_updated":"2023-08-03T14:47:27Z","date_created":"2023-08-03T14:47:27Z","checksum":"1aff850f47ae8c8f05ca48ff9faa38b1","success":1},{"relation":"main_file","file_id":"13918","checksum":"f644de697d142c83461ed5ad1d72a043","success":1,"date_updated":"2023-08-03T14:48:38Z","date_created":"2023-08-03T14:48:38Z","access_level":"open_access","file_name":"Supplfig24_a_i.tif","file_size":44179078,"content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","file_size":44125924,"access_level":"open_access","file_name":"Supplfig24_a_ii.tif","success":1,"checksum":"eccb84925a6699b396d4b07efcbc7349","date_updated":"2023-08-03T14:48:38Z","date_created":"2023-08-03T14:48:38Z","file_id":"13919","relation":"main_file"},{"date_updated":"2023-08-03T14:48:54Z","date_created":"2023-08-03T14:48:54Z","success":1,"checksum":"afe7253e70ec79144e59b28c3c66703a","file_id":"13920","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":1061835435,"file_name":"Supplfig24_a.tif","access_level":"open_access"},{"date_updated":"2023-08-03T14:48:39Z","date_created":"2023-08-03T14:48:39Z","success":1,"checksum":"94a03b022aa84ea6e8515450a481ad82","file_id":"13921","relation":"main_file","creator":"jdanzl","file_size":3977824,"content_type":"image/tiff","file_name":"Supplfig24_b_iii.tif","access_level":"open_access"},{"access_level":"open_access","file_name":"Supplfig24_b_iv.tif","file_size":3977825,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13922","checksum":"166832c173fcda88ec6e35decdf0d197","success":1,"date_updated":"2023-08-03T14:48:39Z","date_created":"2023-08-03T14:48:39Z"},{"file_id":"13923","relation":"main_file","date_updated":"2023-08-03T14:51:27Z","date_created":"2023-08-03T14:51:27Z","success":1,"checksum":"53175073b83ab569241ae64ef7f180b3","file_name":"Supplfig25_a_1.tif","access_level":"open_access","creator":"jdanzl","content_type":"image/tiff","file_size":37641738},{"creator":"jdanzl","content_type":"image/tiff","file_size":4932897,"access_level":"open_access","file_name":"Supplfig25_a_2.tif","success":1,"checksum":"70e6a1780daeae6a5bef2c27c996c442","date_updated":"2023-08-03T14:51:26Z","date_created":"2023-08-03T14:51:26Z","file_id":"13924","relation":"main_file"},{"creator":"jdanzl","file_size":357433570,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig25_b_1.tif","success":1,"checksum":"1748266d7b8a2ae1f6292b9c265c2068","date_updated":"2023-08-03T14:51:31Z","date_created":"2023-08-03T14:51:31Z","file_id":"13925","relation":"main_file"},{"file_size":27734506,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig25_b_2.tif","checksum":"8490f44160a482ea06d858eb3bc25a11","success":1,"date_updated":"2023-08-03T14:51:27Z","date_created":"2023-08-03T14:51:27Z","relation":"main_file","file_id":"13926"},{"relation":"main_file","file_id":"13927","checksum":"e5e9c550cc934a7725dc209c79951847","success":1,"date_updated":"2023-08-03T14:53:43Z","date_created":"2023-08-03T14:53:43Z","access_level":"open_access","file_name":"Suppfig26_h.tif","file_size":35082394,"content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","file_size":16676477,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig26_a_1.tif","success":1,"checksum":"39ae411d1e950e78bdc12937fb36f661","date_created":"2023-08-03T14:53:42Z","date_updated":"2023-08-03T14:53:42Z","file_id":"13928","relation":"main_file"},{"creator":"jdanzl","file_size":170493,"content_type":"image/tiff","access_level":"open_access","file_name":"Supplfig26_a_2.tif","success":1,"checksum":"450f5d0e8091639e27eb7a7991524e88","date_updated":"2023-08-03T14:53:42Z","date_created":"2023-08-03T14:53:42Z","file_id":"13929","relation":"main_file"},{"file_name":"Supplfig26_b_1.tif","access_level":"open_access","file_size":11117737,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13930","date_updated":"2023-08-03T14:53:43Z","date_created":"2023-08-03T14:53:43Z","checksum":"8e5a71801fa1fcb7f679d1af23344662","success":1},{"checksum":"519fb0326a0f0774b3edc1dafffcdd08","success":1,"date_updated":"2023-08-03T14:53:43Z","date_created":"2023-08-03T14:53:43Z","relation":"main_file","file_id":"13931","file_size":113753,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig26_b_2.tif"},{"file_id":"13932","relation":"main_file","success":1,"checksum":"d051c7d717eeb6f2ca59d8727ea62200","date_updated":"2023-08-03T14:53:52Z","date_created":"2023-08-03T14:53:52Z","access_level":"open_access","file_name":"Supplfig26_c.tif","creator":"jdanzl","content_type":"image/tiff","file_size":495452386},{"file_name":"Supplfig26_d.tif","access_level":"open_access","creator":"jdanzl","file_size":132167170,"content_type":"image/tiff","file_id":"13933","relation":"main_file","date_created":"2023-08-03T14:53:46Z","date_updated":"2023-08-03T14:53:46Z","success":1,"checksum":"5437b53e708572e2e9ad1432c260a04f"},{"content_type":"image/tiff","file_size":11431214,"creator":"jdanzl","file_name":"Supplfig26_e.tif","access_level":"open_access","date_created":"2023-08-03T14:53:46Z","date_updated":"2023-08-03T14:53:46Z","checksum":"0baab38829a8587ded5e39f85068124b","success":1,"relation":"main_file","file_id":"13934"},{"access_level":"open_access","file_name":"Supplfig26_f.tif","content_type":"image/tiff","file_size":64005702,"creator":"jdanzl","relation":"main_file","file_id":"13935","checksum":"afa57f254c9dd987d90d5e43db3fb9e6","success":1,"date_created":"2023-08-03T14:53:48Z","date_updated":"2023-08-03T14:53:48Z"},{"file_id":"13936","relation":"main_file","success":1,"checksum":"c35ba67ab92c410cb4370f91f3dea819","date_updated":"2023-08-03T14:53:52Z","date_created":"2023-08-03T14:53:52Z","access_level":"open_access","file_name":"Supplfig26_g.tif","creator":"jdanzl","file_size":246421054,"content_type":"image/tiff"},{"file_name":"Supplfig27_a.tif","access_level":"open_access","file_size":166614932,"content_type":"image/tiff","creator":"jdanzl","relation":"main_file","file_id":"13937","date_created":"2023-08-03T14:56:43Z","date_updated":"2023-08-03T14:56:43Z","checksum":"3fd6186d73cbce1be79cf14a94a7f3bd","success":1},{"date_created":"2023-08-03T14:56:42Z","date_updated":"2023-08-03T14:56:42Z","success":1,"checksum":"d1fc4c88e8e258e4e536a26e196d09b0","file_id":"13938","relation":"main_file","creator":"jdanzl","file_size":143583446,"content_type":"image/tiff","file_name":"Supplfig27_b.tif","access_level":"open_access"},{"date_updated":"2023-08-03T14:56:47Z","date_created":"2023-08-03T14:56:47Z","success":1,"checksum":"8d6985530e86f38548e89e6380d73771","file_id":"13939","relation":"main_file","creator":"jdanzl","file_size":172528188,"content_type":"image/tiff","file_name":"Supplfig27_c.tif","access_level":"open_access"},{"access_level":"open_access","file_name":"Supplfig27_d.tif","content_type":"image/tiff","file_size":113699256,"creator":"jdanzl","relation":"main_file","file_id":"13940","checksum":"d0b1309d2120892ede84e04fdf0dbfbf","success":1,"date_updated":"2023-08-03T14:56:46Z","date_created":"2023-08-03T14:56:46Z"},{"content_type":"image/tiff","file_size":88664548,"creator":"jdanzl","file_name":"Supplfig27_e.tif","access_level":"open_access","date_created":"2023-08-03T14:56:47Z","date_updated":"2023-08-03T14:56:47Z","checksum":"4789b5254d0415ce314fc9e39cc52121","success":1,"relation":"main_file","file_id":"13941"},{"file_size":2298356,"content_type":"image/tiff","creator":"jdanzl","access_level":"open_access","file_name":"Supplfig27_f.tif","checksum":"7ff2d06534f062b0f17dab181f6c1f4e","success":1,"date_created":"2023-08-03T14:56:47Z","date_updated":"2023-08-03T14:56:47Z","relation":"main_file","file_id":"13942"},{"success":1,"checksum":"46de66d457b0def90f753c11927536b7","date_created":"2023-08-03T14:56:47Z","date_updated":"2023-08-03T14:56:47Z","file_id":"13943","relation":"main_file","creator":"jdanzl","content_type":"image/tiff","file_size":499618,"access_level":"open_access","file_name":"Supplfig27_g_1.tif"},{"file_id":"13944","relation":"main_file","success":1,"checksum":"9ef5fba064a73a79dbc74b5e166d08ec","date_updated":"2023-08-03T14:56:50Z","date_created":"2023-08-03T14:56:50Z","access_level":"open_access","file_name":"Supplfig27_g_2.tif","creator":"jdanzl","file_size":202079026,"content_type":"image/tiff"},{"relation":"main_file","file_id":"13945","checksum":"bf956c2b58d3da2baa869db5206d8d49","success":1,"date_updated":"2023-08-03T14:56:48Z","date_created":"2023-08-03T14:56:48Z","access_level":"open_access","file_name":"Supplfig27_g_3.tif","file_size":14639269,"content_type":"image/tiff","creator":"jdanzl"},{"file_name":"model.p","access_level":"open_access","content_type":"application/octet-stream","file_size":279467712,"creator":"jdanzl","relation":"main_file","file_id":"13946","date_updated":"2023-08-03T15:21:01Z","date_created":"2023-08-03T15:21:01Z","checksum":"327dca11f42d5375d7f47f4cbd5b36c0","success":1}]},{"article_type":"original","publication":"Nature Biotechnology","citation":{"ieee":"J. M. Michalska et al., “Imaging brain tissue architecture across millimeter to nanometer scales,” Nature Biotechnology. Springer Nature, 2023.","apa":"Michalska, J. M., Lyudchik, J., Velicky, P., Korinkova, H., Watson, J., Cenameri, A., … Danzl, J. G. (2023). Imaging brain tissue architecture across millimeter to nanometer scales. Nature Biotechnology. Springer Nature. https://doi.org/10.1038/s41587-023-01911-8","ista":"Michalska JM, Lyudchik J, Velicky P, Korinkova H, Watson J, Cenameri A, Sommer CM, Amberg N, Venturino A, Roessler K, Czech T, Höftberger R, Siegert S, Novarino G, Jonas PM, Danzl JG. 2023. Imaging brain tissue architecture across millimeter to nanometer scales. Nature Biotechnology.","ama":"Michalska JM, Lyudchik J, Velicky P, et al. Imaging brain tissue architecture across millimeter to nanometer scales. Nature Biotechnology. 2023. doi:10.1038/s41587-023-01911-8","chicago":"Michalska, Julia M, Julia Lyudchik, Philipp Velicky, Hana Korinkova, Jake Watson, Alban Cenameri, Christoph M Sommer, et al. “Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.” Nature Biotechnology. Springer Nature, 2023. https://doi.org/10.1038/s41587-023-01911-8.","short":"J.M. Michalska, J. Lyudchik, P. Velicky, H. Korinkova, J. Watson, A. Cenameri, C.M. Sommer, N. Amberg, A. Venturino, K. Roessler, T. Czech, R. Höftberger, S. Siegert, G. Novarino, P.M. Jonas, J.G. Danzl, Nature Biotechnology (2023).","mla":"Michalska, Julia M., et al. “Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.” Nature Biotechnology, Springer Nature, 2023, doi:10.1038/s41587-023-01911-8."},"date_published":"2023-08-31T00:00:00Z","scopus_import":"1","day":"31","article_processing_charge":"Yes (in subscription journal)","title":"Imaging brain tissue architecture across millimeter to nanometer scales","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"14257","oa_version":"Published Version","type":"journal_article","abstract":[{"text":"Mapping the complex and dense arrangement of cells and their connectivity in brain tissue demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels at visualizing specific molecules and individual cells but fails to provide tissue context. Here we developed Comprehensive Analysis of Tissues across Scales (CATS), a technology to densely map brain tissue architecture from millimeter regional to nanometer synaptic scales in diverse chemically fixed brain preparations, including rodent and human. CATS uses fixation-compatible extracellular labeling and optical imaging, including stimulated emission depletion or expansion microscopy, to comprehensively delineate cellular structures. It enables three-dimensional reconstruction of single synapses and mapping of synaptic connectivity by identification and analysis of putative synaptic cleft regions. Applying CATS to the mouse hippocampal mossy fiber circuitry, we reconstructed and quantified the synaptic input and output structure of identified neurons. We furthermore demonstrate applicability to clinically derived human tissue samples, including formalin-fixed paraffin-embedded routine diagnostic specimens, for visualizing the cellular architecture of brain tissue in health and disease.","lang":"eng"}],"isi":1,"quality_controlled":"1","project":[{"grant_number":"I03600","_id":"265CB4D0-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Optical control of synaptic function via adhesion molecules"},{"grant_number":"W1232-B24","_id":"2548AE96-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets","call_identifier":"FWF"},{"name":"The Wittgenstein Prize","call_identifier":"FWF","grant_number":"Z00312","_id":"25C5A090-B435-11E9-9278-68D0E5697425"},{"_id":"23889792-32DE-11EA-91FC-C7463DDC885E","name":"High content imaging to decode human immune cell interactions in health and allergic disease"},{"call_identifier":"H2020","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","grant_number":"715508","_id":"25444568-B435-11E9-9278-68D0E5697425"},{"name":"Biophysics and circuit function of a giant cortical glumatergic synapse","call_identifier":"H2020","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","grant_number":"692692"},{"call_identifier":"H2020","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385"},{"_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","grant_number":"101026635","call_identifier":"H2020","name":"Synaptic computations of the hippocampal CA3 circuitry"}],"oa":1,"external_id":{"isi":["001065254200001"]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41587-023-01911-8"}],"acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"language":[{"iso":"eng"}],"doi":"10.1038/s41587-023-01911-8","month":"08","publication_identifier":{"issn":["1087-0156"],"eissn":["1546-1696"]},"publication_status":"epub_ahead","department":[{"_id":"SaSi"},{"_id":"GaNo"},{"_id":"PeJo"},{"_id":"JoDa"},{"_id":"Bio"},{"_id":"RySh"}],"publisher":"Springer Nature","year":"2023","acknowledgement":"We thank J. Vorlaufer, N. Agudelo-Dueñas, W. Jahr and A. Wartak for microscope maintenance and troubleshooting; C. Kreuzinger, A. Freeman and I. Erber for technical assistance; and M. Tomschik for support with obtaining human samples. We gratefully acknowledge E. Miguel for setting up webKnossos and M. Šuplata for computational support and hardware control. We are grateful to R. Shigemoto and B. Bickel for generous support and M. Sixt and S. Boyd (Stanford University) for discussions and critical reading of the paper. PSD95-HaloTag mice were kindly provided by S. Grant (University of Edinburgh). We acknowledge expert support by Institute of Science and Technology Austria’s scientific computing, imaging and optics, preclinical and lab support facilities and by the Miba machine shop and library. We gratefully acknowledge funding by the following sources: Austrian Science Fund (FWF) grant I3600-B27 (J.G.D.); Austrian Science Fund (FWF) grant DK W1232 (J.G.D. and J.M.M.); Austrian Science Fund (FWF) grant Z 312-B27, Wittgenstein award (P.J.); Austrian Science Fund (FWF) projects I4685-B, I6565-B (SYNABS) and DOC 33-B27 (R.H.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); European Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 715508 – REVERSEAUTISM (G.N.); European Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 692692 – GIANTSYN (P.J.); Marie Skłodowska-Curie Actions Fellowship GA no. 665385 under the EU Horizon 2020 program (J.M.M. and J.L.); and Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635 under the EU Horizon 2020 program (J.F.W.).","date_updated":"2024-02-21T12:18:18Z","date_created":"2023-09-03T22:01:15Z","author":[{"full_name":"Michalska, Julia M","first_name":"Julia M","last_name":"Michalska","id":"443DB6DE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3862-1235"},{"full_name":"Lyudchik, Julia","id":"46E28B80-F248-11E8-B48F-1D18A9856A87","last_name":"Lyudchik","first_name":"Julia"},{"first_name":"Philipp","last_name":"Velicky","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2340-7431","full_name":"Velicky, Philipp"},{"full_name":"Korinkova, Hana","id":"ee3cb6ca-ec98-11ea-ae11-ff703e2254ed","first_name":"Hana","last_name":"Korinkova"},{"full_name":"Watson, Jake","first_name":"Jake","last_name":"Watson","id":"63836096-4690-11EA-BD4E-32803DDC885E","orcid":"0000-0002-8698-3823"},{"full_name":"Cenameri, Alban","last_name":"Cenameri","first_name":"Alban","id":"9ac8f577-2357-11eb-997a-e566c5550886"},{"full_name":"Sommer, Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","first_name":"Christoph M","last_name":"Sommer"},{"id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3183-8207","first_name":"Nicole","last_name":"Amberg","full_name":"Amberg, Nicole"},{"id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2356-9403","first_name":"Alessandro","last_name":"Venturino","full_name":"Venturino, Alessandro"},{"full_name":"Roessler, Karl","last_name":"Roessler","first_name":"Karl"},{"first_name":"Thomas","last_name":"Czech","full_name":"Czech, Thomas"},{"first_name":"Romana","last_name":"Höftberger","full_name":"Höftberger, Romana"},{"full_name":"Siegert, Sandra","last_name":"Siegert","first_name":"Sandra","orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Novarino","first_name":"Gaia","orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","full_name":"Novarino, Gaia"},{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804","first_name":"Peter M","last_name":"Jonas","full_name":"Jonas, Peter M"},{"id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8559-3973","first_name":"Johann G","last_name":"Danzl","full_name":"Danzl, Johann G"}],"related_material":{"link":[{"relation":"software","url":"https://github.com/danzllab/CATS"}],"record":[{"id":"13126","relation":"research_data","status":"public"}]},"ec_funded":1},{"publication_identifier":{"issn":["2663-337X"]},"month":"08","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"}],"supervisor":[{"last_name":"Siegert","first_name":"Sandra","orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","full_name":"Siegert, Sandra"}],"degree_awarded":"PhD","doi":"10.15479/at:ista:11945","project":[{"_id":"267F75D8-B435-11E9-9278-68D0E5697425","name":"Modulating microglia through G protein-coupled receptor (GPCR) signaling"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"file_date_updated":"2022-08-25T09:33:31Z","date_updated":"2023-08-03T13:02:26Z","date_created":"2022-08-23T11:33:11Z","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"11995"}]},"author":[{"full_name":"Schulz, Rouven","first_name":"Rouven","last_name":"Schulz","id":"4C5E7B96-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5297-733X"}],"department":[{"_id":"GradSch"},{"_id":"SaSi"}],"publisher":"Institute of Science and Technology Austria","publication_status":"published","year":"2022","has_accepted_license":"1","article_processing_charge":"No","day":"23","date_published":"2022-08-23T00:00:00Z","page":"133","citation":{"mla":"Schulz, Rouven. Chimeric G Protein-Coupled Receptors Mimic Distinct Signaling Pathways and Modulate Microglia Function. Institute of Science and Technology Austria, 2022, doi:10.15479/at:ista:11945.","short":"R. Schulz, Chimeric G Protein-Coupled Receptors Mimic Distinct Signaling Pathways and Modulate Microglia Function, Institute of Science and Technology Austria, 2022.","chicago":"Schulz, Rouven. “Chimeric G Protein-Coupled Receptors Mimic Distinct Signaling Pathways and Modulate Microglia Function.” Institute of Science and Technology Austria, 2022. https://doi.org/10.15479/at:ista:11945.","ama":"Schulz R. Chimeric G protein-coupled receptors mimic distinct signaling pathways and modulate microglia function. 2022. doi:10.15479/at:ista:11945","ista":"Schulz R. 2022. Chimeric G protein-coupled receptors mimic distinct signaling pathways and modulate microglia function. Institute of Science and Technology Austria.","ieee":"R. Schulz, “Chimeric G protein-coupled receptors mimic distinct signaling pathways and modulate microglia function,” Institute of Science and Technology Austria, 2022.","apa":"Schulz, R. (2022). Chimeric G protein-coupled receptors mimic distinct signaling pathways and modulate microglia function. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:11945"},"abstract":[{"lang":"eng","text":"G protein-coupled receptors (GPCRs) respond to specific ligands and regulate multiple processes ranging from cell growth and immune responses to neuronal signal transmission. However, ligands for many GPCRs remain unknown, suffer from off-target effects or have poor bioavailability. Additional challenges exist to dissect cell-type specific responses when the same GPCR is expressed on several cell types within the body. Here, we overcome these limitations by engineering DREADD-based GPCR chimeras that selectively bind their agonist clozapine-N-oxide (CNO) and mimic a GPCR-of-interest in a desired cell type.\r\nWe validated our approach with β2-adrenergic receptor (β2AR/ADRB2) and show that our chimeric DREADD-β2AR triggers comparable responses on second messenger and kinase activity, post-translational modifications, and protein-protein interactions. Since β2AR is also enriched in microglia, which can drive inflammation in the central nervous system, we expressed chimeric DREADD-β2AR in primary microglia and successfully recapitulate β2AR-mediated filopodia formation through CNO stimulation. To dissect the role of selected GPCRs during microglial inflammation, we additionally generated DREADD-based chimeras for microglia-enriched GPR65 and GPR109A/HCAR2. In a microglia cell line, DREADD-β2AR and DREADD-GPR65 both modulated the inflammatory response with a similar profile as endogenously expressed β2AR, while DREADD-GPR109A showed no impact.\r\nOur DREADD-based approach provides the means to obtain mechanistic and functional insights into GPCR signaling on a cell-type specific level."}],"alternative_title":["ISTA Thesis"],"type":"dissertation","oa_version":"Published Version","file":[{"checksum":"61b1b666a210ff7cdd0e95ea75207a13","success":1,"date_updated":"2022-08-25T08:59:57Z","date_created":"2022-08-25T08:59:57Z","relation":"main_file","file_id":"11970","content_type":"application/pdf","file_size":28079331,"creator":"rschulz","access_level":"open_access","file_name":"Thesis_Rouven_Schulz_2022_final.pdf"},{"file_name":"Thesis_Rouven_Schulz_2022_final.docx","access_level":"closed","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_size":27226963,"creator":"rschulz","relation":"source_file","file_id":"11971","date_created":"2022-08-25T09:00:11Z","date_updated":"2022-08-25T09:33:31Z","checksum":"2b8f95ea1c134dbdb927b41b1dbeeeb5"}],"status":"public","ddc":["570"],"title":"Chimeric G protein-coupled receptors mimic distinct signaling pathways and modulate microglia function","_id":"11945","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9"},{"article_type":"original","publication":"iScience","citation":{"ista":"Bartalska K, Hübschmann V, Korkut M, Cubero RJ, Venturino A, Rössler K, Czech T, Siegert S. 2022. A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. iScience. 25(7), 104580.","apa":"Bartalska, K., Hübschmann, V., Korkut, M., Cubero, R. J., Venturino, A., Rössler, K., … Siegert, S. (2022). A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. IScience. Elsevier. https://doi.org/10.1016/j.isci.2022.104580","ieee":"K. Bartalska et al., “A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation,” iScience, vol. 25, no. 7. Elsevier, 2022.","ama":"Bartalska K, Hübschmann V, Korkut M, et al. A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. iScience. 2022;25(7). doi:10.1016/j.isci.2022.104580","chicago":"Bartalska, Katarina, Verena Hübschmann, Medina Korkut, Ryan J Cubero, Alessandro Venturino, Karl Rössler, Thomas Czech, and Sandra Siegert. “A Systematic Characterization of Microglia-like Cell Occurrence during Retinal Organoid Differentiation.” IScience. Elsevier, 2022. https://doi.org/10.1016/j.isci.2022.104580.","mla":"Bartalska, Katarina, et al. “A Systematic Characterization of Microglia-like Cell Occurrence during Retinal Organoid Differentiation.” IScience, vol. 25, no. 7, 104580, Elsevier, 2022, doi:10.1016/j.isci.2022.104580.","short":"K. Bartalska, V. Hübschmann, M. Korkut, R.J. Cubero, A. Venturino, K. Rössler, T. Czech, S. Siegert, IScience 25 (2022)."},"date_published":"2022-07-15T00:00:00Z","scopus_import":"1","day":"15","has_accepted_license":"1","article_processing_charge":"Yes","ddc":["610"],"status":"public","title":"A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation","intvolume":" 25","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"11478","file":[{"checksum":"a470b74e1b3796c710189c81a4cd4329","success":1,"date_updated":"2022-07-04T08:19:25Z","date_created":"2022-07-04T08:19:25Z","relation":"main_file","file_id":"11480","content_type":"application/pdf","file_size":19400048,"creator":"cchlebak","access_level":"open_access","file_name":"2022_iScience_Bartalska.pdf"}],"oa_version":"Published Version","type":"journal_article","abstract":[{"text":"Cerebral organoids differentiated from human-induced pluripotent stem cells (hiPSC) provide a unique opportunity to investigate brain development. However, organoids usually lack microglia, brain-resident immune cells, which are present in the early embryonic brain and participate in neuronal circuit development. Here, we find IBA1+ microglia-like cells alongside retinal cups between week 3 and 4 in 2.5D culture with an unguided retinal organoid differentiation protocol. Microglia do not infiltrate the neuroectoderm and instead enrich within non-pigmented, 3D-cystic compartments that develop in parallel to the 3D-retinal organoids. When we guide the retinal organoid differentiation with low-dosed BMP4, we prevent cup development and enhance microglia and 3D-cysts formation. Mass spectrometry identifies these 3D-cysts to express mesenchymal and epithelial markers. We confirmed this microglia-preferred environment also within the unguided protocol, providing insight into microglial behavior and migration and offer a model to study how they enter and distribute within the human brain.","lang":"eng"}],"issue":"7","isi":1,"quality_controlled":"1","project":[{"grant_number":"715571","_id":"25D4A630-B435-11E9-9278-68D0E5697425","name":"Microglia action towards neuronal circuit formation and function in health and disease","call_identifier":"H2020"},{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"},{"name":"How human microglia shape developing neurons during health and inflammation","_id":"9B99D380-BA93-11EA-9121-9846C619BF3A","grant_number":"SC19-017"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["000830428500005"]},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"language":[{"iso":"eng"}],"doi":"10.1016/j.isci.2022.104580","month":"07","publication_identifier":{"eissn":["2589-0042"]},"publication_status":"published","publisher":"Elsevier","department":[{"_id":"SaSi"}],"year":"2022","acknowledgement":"We thank the scientific service units at ISTA, specifically the lab support facility and imaging & optics facility for their support; Nicolas Armel for performing the Mass Spectrometry. We thank Alexandra Lang and Tanja Peilnsteiner for their help in human brain tissue collection, Rouven Schulz for his insights into the functional assays We thank all members of the Siegert group for constant feedback on the project and Margaret Maes, Rouven Schulz, and Marco Benevento for feedback on the manuscript. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant No. 715571 to S.S.) and from the Gesellschaft für Forschungsförderung Niederösterreich (grant No. Sc19-017 to V.H.).","date_updated":"2023-11-02T12:21:33Z","date_created":"2022-07-03T22:01:33Z","volume":25,"author":[{"last_name":"Bartalska","first_name":"Katarina","id":"4D883232-F248-11E8-B48F-1D18A9856A87","full_name":"Bartalska, Katarina"},{"id":"32B7C918-F248-11E8-B48F-1D18A9856A87","first_name":"Verena","last_name":"Hübschmann","full_name":"Hübschmann, Verena"},{"full_name":"Korkut, Medina","orcid":"0000-0003-4309-2251","id":"4B51CE74-F248-11E8-B48F-1D18A9856A87","last_name":"Korkut","first_name":"Medina"},{"first_name":"Ryan J","last_name":"Cubero","id":"850B2E12-9CD4-11E9-837F-E719E6697425","orcid":"0000-0003-0002-1867","full_name":"Cubero, Ryan J"},{"orcid":"0000-0003-2356-9403","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","last_name":"Venturino","first_name":"Alessandro","full_name":"Venturino, Alessandro"},{"full_name":"Rössler, Karl","first_name":"Karl","last_name":"Rössler"},{"first_name":"Thomas","last_name":"Czech","full_name":"Czech, Thomas"},{"last_name":"Siegert","first_name":"Sandra","orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","full_name":"Siegert, Sandra"}],"related_material":{"record":[{"relation":"other","status":"public","id":"12117"}]},"article_number":"104580","file_date_updated":"2022-07-04T08:19:25Z","ec_funded":1},{"publication":"STAR Protocols","citation":{"chicago":"Hübschmann, Verena, Medina Korkut, and Sandra Siegert. “Assessing Human IPSC-Derived Microglia Identity and Function by Immunostaining, Phagocytosis, Calcium Activity, and Inflammation Assay.” STAR Protocols. Elsevier, 2022. https://doi.org/10.1016/j.xpro.2022.101866.","short":"V. Hübschmann, M. Korkut, S. Siegert, STAR Protocols 3 (2022).","mla":"Hübschmann, Verena, et al. “Assessing Human IPSC-Derived Microglia Identity and Function by Immunostaining, Phagocytosis, Calcium Activity, and Inflammation Assay.” STAR Protocols, vol. 3, no. 4, 101866, Elsevier, 2022, doi:10.1016/j.xpro.2022.101866.","apa":"Hübschmann, V., Korkut, M., & Siegert, S. (2022). Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay. STAR Protocols. Elsevier. https://doi.org/10.1016/j.xpro.2022.101866","ieee":"V. Hübschmann, M. Korkut, and S. Siegert, “Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay,” STAR Protocols, vol. 3, no. 4. Elsevier, 2022.","ista":"Hübschmann V, Korkut M, Siegert S. 2022. Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay. STAR Protocols. 3(4), 101866.","ama":"Hübschmann V, Korkut M, Siegert S. Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay. STAR Protocols. 2022;3(4). doi:10.1016/j.xpro.2022.101866"},"article_type":"letter_note","date_published":"2022-12-16T00:00:00Z","scopus_import":"1","keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","General Neuroscience"],"day":"16","has_accepted_license":"1","article_processing_charge":"No","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"12117","ddc":["570"],"title":"Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay","status":"public","intvolume":" 3","file":[{"checksum":"3c71b8a60633d42c2f77c49025d5559b","success":1,"date_created":"2023-01-23T09:50:51Z","date_updated":"2023-01-23T09:50:51Z","relation":"main_file","file_id":"12340","file_size":6251945,"content_type":"application/pdf","creator":"dernst","access_level":"open_access","file_name":"2022_STARProtocols_Huebschmann.pdf"}],"oa_version":"Published Version","type":"journal_article","abstract":[{"text":"To understand how potential gene manipulations affect in vitro microglia, we provide a set of short protocols to evaluate microglia identity and function. We detail steps for immunostaining to determine microglia identity. We describe three functional assays for microglia: phagocytosis, calcium response following ATP stimulation, and cytokine expression upon inflammatory stimuli. We apply these protocols to human induced-pluripotent-stem-cell (hiPSC)-derived microglia, but they can be also applied to other in vitro microglial models including primary mouse microglia.\r\nFor complete details on the use and execution of this protocol, please refer to Bartalska et al. (2022).1","lang":"eng"}],"issue":"4","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"quality_controlled":"1","project":[{"name":"Microglia action towards neuronal circuit formation and function in health and disease","call_identifier":"H2020","grant_number":"715571","_id":"25D4A630-B435-11E9-9278-68D0E5697425"},{"_id":"9B99D380-BA93-11EA-9121-9846C619BF3A","grant_number":"SC19-017","name":"How human microglia shape developing neurons during health and inflammation"}],"doi":"10.1016/j.xpro.2022.101866","acknowledged_ssus":[{"_id":"Bio"}],"language":[{"iso":"eng"}],"month":"12","publication_identifier":{"issn":["2666-1667"]},"acknowledgement":"This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant No. 715571 to S.S.) and from the Gesellschaft für Forschungsförderung Niederösterreich (grant No. Sc19-017 to V.H.). We thank Rouven Schulz and Alessandro Venturino for their insights into functional assays and data analysis, Verena Seiboth for insights into necessary institutional permission, and ISTA imaging & optics facility (IOF) especially Bernhard Hochreiter for their support.","year":"2022","publication_status":"published","publisher":"Elsevier","department":[{"_id":"SaSi"},{"_id":"GradSch"}],"author":[{"last_name":"Hübschmann","first_name":"Verena","id":"32B7C918-F248-11E8-B48F-1D18A9856A87","full_name":"Hübschmann, Verena"},{"orcid":"0000-0003-4309-2251","id":"4B51CE74-F248-11E8-B48F-1D18A9856A87","last_name":"Korkut","first_name":"Medina","full_name":"Korkut, Medina"},{"full_name":"Siegert, Sandra","first_name":"Sandra","last_name":"Siegert","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8635-0877"}],"related_material":{"record":[{"id":"11478","status":"public","relation":"other"}]},"date_created":"2023-01-12T11:56:38Z","date_updated":"2023-11-02T12:21:32Z","volume":3,"article_number":"101866","file_date_updated":"2023-01-23T09:50:51Z","ec_funded":1},{"file":[{"file_id":"12002","relation":"main_file","date_created":"2022-08-29T06:44:30Z","date_updated":"2022-08-29T06:44:30Z","success":1,"checksum":"191d9db0266e14a28d3a56dc7f65da84","file_name":"2022_NatComm_Schulz.pdf","access_level":"open_access","creator":"cchlebak","content_type":"application/pdf","file_size":7317396}],"oa_version":"Published Version","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"11995","title":"Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses","status":"public","ddc":["570"],"intvolume":" 13","abstract":[{"text":"G protein-coupled receptors (GPCRs) regulate processes ranging from immune responses to neuronal signaling. However, ligands for many GPCRs remain unknown, suffer from off-target effects or have poor bioavailability. Additionally, dissecting cell type-specific responses is challenging when the same GPCR is expressed on different cells within a tissue. Here, we overcome these limitations by engineering DREADD-based GPCR chimeras that bind clozapine-N-oxide and mimic a GPCR-of-interest. We show that chimeric DREADD-β2AR triggers responses comparable to β2AR on second messenger and kinase activity, post-translational modifications, and protein-protein interactions. Moreover, we successfully recapitulate β2AR-mediated filopodia formation in microglia, an immune cell capable of driving central nervous system inflammation. When dissecting microglial inflammation, we included two additional DREADD-based chimeras mimicking microglia-enriched GPR65 and GPR109A. DREADD-β2AR and DREADD-GPR65 modulate the inflammatory response with high similarity to endogenous β2AR, while DREADD-GPR109A shows no impact. Our DREADD-based approach allows investigation of cell type-dependent pathways without known endogenous ligands.","lang":"eng"}],"type":"journal_article","date_published":"2022-08-15T00:00:00Z","publication":"Nature Communications","citation":{"ama":"Schulz R, Korkut M, Venturino A, Colombo G, Siegert S. Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses. Nature Communications. 2022;13. doi:10.1038/s41467-022-32390-1","ieee":"R. Schulz, M. Korkut, A. Venturino, G. Colombo, and S. Siegert, “Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses,” Nature Communications, vol. 13. Springer Nature, 2022.","apa":"Schulz, R., Korkut, M., Venturino, A., Colombo, G., & Siegert, S. (2022). Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-022-32390-1","ista":"Schulz R, Korkut M, Venturino A, Colombo G, Siegert S. 2022. Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses. Nature Communications. 13, 4728.","short":"R. Schulz, M. Korkut, A. Venturino, G. Colombo, S. Siegert, Nature Communications 13 (2022).","mla":"Schulz, Rouven, et al. “Chimeric GPCRs Mimic Distinct Signaling Pathways and Modulate Microglia Responses.” Nature Communications, vol. 13, 4728, Springer Nature, 2022, doi:10.1038/s41467-022-32390-1.","chicago":"Schulz, Rouven, Medina Korkut, Alessandro Venturino, Gloria Colombo, and Sandra Siegert. “Chimeric GPCRs Mimic Distinct Signaling Pathways and Modulate Microglia Responses.” Nature Communications. Springer Nature, 2022. https://doi.org/10.1038/s41467-022-32390-1."},"article_type":"original","day":"15","has_accepted_license":"1","article_processing_charge":"No","scopus_import":"1","author":[{"last_name":"Schulz","first_name":"Rouven","orcid":"0000-0001-5297-733X","id":"4C5E7B96-F248-11E8-B48F-1D18A9856A87","full_name":"Schulz, Rouven"},{"full_name":"Korkut, Medina","first_name":"Medina","last_name":"Korkut","id":"4B51CE74-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4309-2251"},{"id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2356-9403","first_name":"Alessandro","last_name":"Venturino","full_name":"Venturino, Alessandro"},{"id":"3483CF6C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9434-8902","first_name":"Gloria","last_name":"Colombo","full_name":"Colombo, Gloria"},{"full_name":"Siegert, Sandra","first_name":"Sandra","last_name":"Siegert","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8635-0877"}],"related_material":{"link":[{"url":"https://ista.ac.at/en/news/dreaddful-mimicry/","relation":"press_release","description":"News on ISTA website"}],"record":[{"id":"11945","relation":"part_of_dissertation","status":"public"},{"status":"public","relation":"research_data","id":"11542"}]},"date_created":"2022-08-28T22:01:59Z","date_updated":"2024-02-21T12:34:51Z","volume":13,"year":"2022","acknowledgement":"The authors thank the Scientific Service Units at ISTA, in particular the Molecular Biology Service of the Lab Support Facility, Imaging & Optics Facility, and the Preclinical Facility, and the Novarino group, Harald Janoviak, and Marco Benevento for sharing reagents and expertise. This research was supported by a DOC Fellowship (24979) awarded to R.S. by the Austrian Academy of Sciences.","pmid":1,"publication_status":"published","department":[{"_id":"SaSi"}],"publisher":"Springer Nature","file_date_updated":"2022-08-29T06:44:30Z","article_number":"4728","doi":"10.1038/s41467-022-32390-1","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"LifeSc"}],"language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"isi":["000840984400032"],"pmid":["35970889"]},"quality_controlled":"1","isi":1,"project":[{"_id":"267F75D8-B435-11E9-9278-68D0E5697425","name":"Modulating microglia through G protein-coupled receptor (GPCR) signaling"}],"month":"08","publication_identifier":{"eissn":["2041-1723"]}},{"article_processing_charge":"No","has_accepted_license":"1","date_published":"2022-01-01T00:00:00Z","doi":"10.15479/AT:ISTA:11542","oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"citation":{"ama":"Schulz R. Source Data (Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses). 2022. doi:10.15479/AT:ISTA:11542","apa":"Schulz, R. (2022). Source Data (Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses). Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:11542","ieee":"R. Schulz, “Source Data (Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses).” Institute of Science and Technology Austria, 2022.","ista":"Schulz R. 2022. Source Data (Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses), Institute of Science and Technology Austria, 10.15479/AT:ISTA:11542.","short":"R. Schulz, (2022).","mla":"Schulz, Rouven. Source Data (Chimeric GPCRs Mimic Distinct Signaling Pathways and Modulate Microglia Responses). Institute of Science and Technology Austria, 2022, doi:10.15479/AT:ISTA:11542.","chicago":"Schulz, Rouven. “Source Data (Chimeric GPCRs Mimic Distinct Signaling Pathways and Modulate Microglia Responses).” Institute of Science and Technology Austria, 2022. https://doi.org/10.15479/AT:ISTA:11542."},"file_date_updated":"2022-07-08T10:56:52Z","type":"research_data","contributor":[{"orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","contributor_type":"contact_person","last_name":"Siegert","first_name":"Sandra"}],"related_material":{"record":[{"id":"11995","status":"public","relation":"used_in_publication"}],"link":[{"relation":"contains","url":"https://www.biorxiv.org/content/10.1101/2021.06.21.449162v1"}]},"author":[{"id":"4C5E7B96-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5297-733X","first_name":"Rouven","last_name":"Schulz","full_name":"Schulz, Rouven"}],"oa_version":"None","file":[{"creator":"rschulz","file_size":135784571,"content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","file_name":"Source Data.xlsx","access_level":"open_access","date_updated":"2022-07-08T10:56:52Z","date_created":"2022-07-08T10:56:52Z","success":1,"checksum":"71e8186583f3adbb6c69a88ac9e6e49b","file_id":"11543","relation":"main_file"}],"date_created":"2022-07-08T11:03:02Z","date_updated":"2024-02-21T12:34:51Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"11542","year":"2022","department":[{"_id":"GradSch"},{"_id":"SaSi"}],"publisher":"Institute of Science and Technology Austria","title":"Source Data (Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses)","status":"public"},{"publication_identifier":{"eissn":["1546-1726"],"issn":["1097-6256"]},"month":"10","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"pmid":["36180790"],"isi":["000862214700001"]},"project":[{"call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"call_identifier":"H2020","name":"Microglia action towards neuronal circuit formation and function in health and disease","grant_number":"715571","_id":"25D4A630-B435-11E9-9278-68D0E5697425"}],"quality_controlled":"1","isi":1,"doi":"10.1038/s41593-022-01167-6","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"ScienComp"}],"ec_funded":1,"file_date_updated":"2023-01-30T08:06:56Z","pmid":1,"acknowledgement":"We thank the scientific service units at ISTA, in particular M. Schunn’s team at the preclinical facility, and especially our colony manager S. Haslinger, for excellent support. We are also grateful to the ISTA Imaging & Optics Facility, and in particular C. Sommer for helping with the data file conversions. We thank R. Erhart from the ISTA Scientific Computing Unit for improving the script performance. We thank M. Maes, B. Nagy, S. Oakeley and M. Benevento and all members of the Siegert group for constant feedback on the project and on the manuscript. This research was supported by the European Union Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Actions program (754411 to R.J.A.C.), and by the European Research Council (grant no. 715571 to S.S.). L.K. was supported by funding to the Blue Brain Project, a research center of the École polytechnique fédérale de Lausanne, from the Swiss government’s ETH Board of the Swiss Federal Institutes of Technology. L.-H.T. was supported by NIH (grant no. R37NS051874) and by the JPB Foundation. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","year":"2022","publisher":"Springer Nature","department":[{"_id":"SaSi"}],"publication_status":"published","related_material":{"link":[{"relation":"press_release","description":"News on ISTA website","url":"https://ista.ac.at/en/news/morphomics-revealing-the-hidden-meaning-of-microglia-shape/"}],"record":[{"id":"12378","relation":"dissertation_contains","status":"public"}]},"author":[{"full_name":"Colombo, Gloria","last_name":"Colombo","first_name":"Gloria","orcid":"0000-0001-9434-8902","id":"3483CF6C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Cubero","first_name":"Ryan J","orcid":"0000-0003-0002-1867","id":"850B2E12-9CD4-11E9-837F-E719E6697425","full_name":"Cubero, Ryan J"},{"full_name":"Kanari, Lida","first_name":"Lida","last_name":"Kanari"},{"first_name":"Alessandro","last_name":"Venturino","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2356-9403","full_name":"Venturino, Alessandro"},{"full_name":"Schulz, Rouven","first_name":"Rouven","last_name":"Schulz","id":"4C5E7B96-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5297-733X"},{"full_name":"Scolamiero, Martina","last_name":"Scolamiero","first_name":"Martina"},{"full_name":"Agerberg, Jens","first_name":"Jens","last_name":"Agerberg"},{"full_name":"Mathys, Hansruedi","last_name":"Mathys","first_name":"Hansruedi"},{"full_name":"Tsai, Li-Huei","first_name":"Li-Huei","last_name":"Tsai"},{"full_name":"Chachólski, Wojciech","last_name":"Chachólski","first_name":"Wojciech"},{"full_name":"Hess, Kathryn","first_name":"Kathryn","last_name":"Hess"},{"id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8635-0877","first_name":"Sandra","last_name":"Siegert","full_name":"Siegert, Sandra"}],"volume":25,"date_created":"2023-01-16T09:53:07Z","date_updated":"2024-03-28T23:30:18Z","scopus_import":"1","keyword":["General Neuroscience"],"article_processing_charge":"No","has_accepted_license":"1","day":"01","citation":{"ama":"Colombo G, Cubero RJ, Kanari L, et al. A tool for mapping microglial morphology, morphOMICs, reveals brain-region and sex-dependent phenotypes. Nature Neuroscience. 2022;25(10):1379-1393. doi:10.1038/s41593-022-01167-6","ista":"Colombo G, Cubero RJ, Kanari L, Venturino A, Schulz R, Scolamiero M, Agerberg J, Mathys H, Tsai L-H, Chachólski W, Hess K, Siegert S. 2022. A tool for mapping microglial morphology, morphOMICs, reveals brain-region and sex-dependent phenotypes. Nature Neuroscience. 25(10), 1379–1393.","apa":"Colombo, G., Cubero, R. J., Kanari, L., Venturino, A., Schulz, R., Scolamiero, M., … Siegert, S. (2022). A tool for mapping microglial morphology, morphOMICs, reveals brain-region and sex-dependent phenotypes. Nature Neuroscience. Springer Nature. https://doi.org/10.1038/s41593-022-01167-6","ieee":"G. Colombo et al., “A tool for mapping microglial morphology, morphOMICs, reveals brain-region and sex-dependent phenotypes,” Nature Neuroscience, vol. 25, no. 10. Springer Nature, pp. 1379–1393, 2022.","mla":"Colombo, Gloria, et al. “A Tool for Mapping Microglial Morphology, MorphOMICs, Reveals Brain-Region and Sex-Dependent Phenotypes.” Nature Neuroscience, vol. 25, no. 10, Springer Nature, 2022, pp. 1379–93, doi:10.1038/s41593-022-01167-6.","short":"G. Colombo, R.J. Cubero, L. Kanari, A. Venturino, R. Schulz, M. Scolamiero, J. Agerberg, H. Mathys, L.-H. Tsai, W. Chachólski, K. Hess, S. Siegert, Nature Neuroscience 25 (2022) 1379–1393.","chicago":"Colombo, Gloria, Ryan J Cubero, Lida Kanari, Alessandro Venturino, Rouven Schulz, Martina Scolamiero, Jens Agerberg, et al. “A Tool for Mapping Microglial Morphology, MorphOMICs, Reveals Brain-Region and Sex-Dependent Phenotypes.” Nature Neuroscience. Springer Nature, 2022. https://doi.org/10.1038/s41593-022-01167-6."},"publication":"Nature Neuroscience","page":"1379-1393","article_type":"original","date_published":"2022-10-01T00:00:00Z","type":"journal_article","issue":"10","abstract":[{"lang":"eng","text":"Environmental cues influence the highly dynamic morphology of microglia. Strategies to characterize these changes usually involve user-selected morphometric features, which preclude the identification of a spectrum of context-dependent morphological phenotypes. Here we develop MorphOMICs, a topological data analysis approach, which enables semiautomatic mapping of microglial morphology into an atlas of cue-dependent phenotypes and overcomes feature-selection biases and biological variability. We extract spatially heterogeneous and sexually dimorphic morphological phenotypes for seven adult mouse brain regions. This sex-specific phenotype declines with maturation but increases over the disease trajectories in two neurodegeneration mouse models, with females showing a faster morphological shift in affected brain regions. Remarkably, microglia morphologies reflect an adaptation upon repeated exposure to ketamine anesthesia and do not recover to control morphologies. Finally, we demonstrate that both long primary processes and short terminal processes provide distinct insights to morphological phenotypes. MorphOMICs opens a new perspective to characterize microglial morphology."}],"_id":"12244","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","intvolume":" 25","title":"A tool for mapping microglial morphology, morphOMICs, reveals brain-region and sex-dependent phenotypes","status":"public","ddc":["570"],"oa_version":"Published Version","file":[{"file_name":"2022_NatureNeuroscience_Colombo.pdf","access_level":"open_access","file_size":23789835,"content_type":"application/pdf","creator":"dernst","relation":"main_file","file_id":"12437","date_updated":"2023-01-30T08:06:56Z","date_created":"2023-01-30T08:06:56Z","checksum":"28431146873096f52e0107b534f178c9","success":1}]},{"ec_funded":1,"file_date_updated":"2023-04-12T22:30:03Z","related_material":{"record":[{"id":"12244","status":"public","relation":"part_of_dissertation"}]},"author":[{"full_name":"Colombo, Gloria","last_name":"Colombo","first_name":"Gloria","orcid":"0000-0001-9434-8902","id":"3483CF6C-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2023-01-25T14:27:43Z","date_updated":"2023-08-04T09:40:37Z","year":"2022","publisher":"Institute of Science and Technology Austria","department":[{"_id":"GradSch"},{"_id":"SaSi"}],"publication_status":"published","publication_identifier":{"issn":["2663-337X"]},"month":"11","doi":"10.15479/at:ista:12378","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"ScienComp"}],"degree_awarded":"PhD","supervisor":[{"last_name":"Siegert","first_name":"Sandra","orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","full_name":"Siegert, Sandra"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"project":[{"call_identifier":"H2020","name":"International IST Doctoral Program","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385"}],"abstract":[{"lang":"eng","text":"Environmental cues influence the highly dynamic morphology of microglia. Strategies to \r\ncharacterize these changes usually involve user-selected morphometric features, which \r\npreclude the identification of a spectrum of context-dependent morphological phenotypes. \r\nHere, we develop MorphOMICs, a topological data analysis approach, which enables semi\u0002automatic mapping of microglial morphology into an atlas of cue-dependent phenotypes,\r\novercomes feature-selection bias and minimizes biological variability. \r\nFirst, with MorphOMICs we derive the morphological spectrum of microglia across seven \r\nbrain regions during postnatal development and in two distinct Alzheimer’s disease \r\ndegeneration mouse models. We uncover region-specific and sexually dimorphic\r\nmorphological trajectories, with females showing an earlier morphological shift than males in \r\nthe degenerating brain. Overall, we demonstrate that both long primary- and short terminal \r\nprocesses provide distinct insights to morphological phenotypes. Moreover, using machine \r\nlearning to map novel condition on the spectrum, we observe that microglia morphologies \r\nreflect a dose-dependent adaptation upon ketamine anesthesia and do not recover to control \r\nmorphologies.\r\nNext, we took advantage of MorphOMICs to build a high-resolution and layer-specific map of \r\nmicroglial morphological spectrum in the retina, covering postnatal development and rd10 \r\ndegeneration. Here, following photoreceptor death, microglia assume an early development\u0002like morphology. Finally, we map microglial morphology following optic nerve crush on the \r\nretinal spectrum and observe a layer- and sex-dependent response. \r\nOverall, MorphOMICs opens a new perspective to analyze microglial morphology across \r\nmultiple conditions, and provides a novel tool to characterize microglial morphology beyond \r\nthe traditionally dichotomized view of microglia."}],"type":"dissertation","alternative_title":["ISTA Thesis"],"oa_version":"Published Version","file":[{"creator":"cchlebak","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_size":23890382,"access_level":"closed","file_name":"Gloria_Colombo_Thesis.docx","embargo_to":"open_access","checksum":"8cd3ddfe9b53381dcf086023d8d8893a","date_created":"2023-01-25T14:31:32Z","date_updated":"2023-04-12T22:30:03Z","file_id":"12379","relation":"source_file"},{"relation":"main_file","file_id":"12380","embargo":"2023-04-11","checksum":"8af4319c18b516e8758e9a6cb02b103b","date_updated":"2023-04-12T22:30:03Z","date_created":"2023-01-25T14:31:36Z","access_level":"open_access","file_name":"Gloria_Colombo_Thesis.pdf","content_type":"application/pdf","file_size":13802421,"creator":"cchlebak"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"12378","status":"public","title":"MorphOMICs, a tool for mapping microglial morphology, reveals brain region- and sex-dependent phenotypes","ddc":["570"],"has_accepted_license":"1","article_processing_charge":"No","day":"11","date_published":"2022-11-11T00:00:00Z","citation":{"short":"G. Colombo, MorphOMICs, a Tool for Mapping Microglial Morphology, Reveals Brain Region- and Sex-Dependent Phenotypes, Institute of Science and Technology Austria, 2022.","mla":"Colombo, Gloria. MorphOMICs, a Tool for Mapping Microglial Morphology, Reveals Brain Region- and Sex-Dependent Phenotypes. Institute of Science and Technology Austria, 2022, doi:10.15479/at:ista:12378.","chicago":"Colombo, Gloria. “MorphOMICs, a Tool for Mapping Microglial Morphology, Reveals Brain Region- and Sex-Dependent Phenotypes.” Institute of Science and Technology Austria, 2022. https://doi.org/10.15479/at:ista:12378.","ama":"Colombo G. MorphOMICs, a tool for mapping microglial morphology, reveals brain region- and sex-dependent phenotypes. 2022. doi:10.15479/at:ista:12378","ieee":"G. Colombo, “MorphOMICs, a tool for mapping microglial morphology, reveals brain region- and sex-dependent phenotypes,” Institute of Science and Technology Austria, 2022.","apa":"Colombo, G. (2022). MorphOMICs, a tool for mapping microglial morphology, reveals brain region- and sex-dependent phenotypes. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12378","ista":"Colombo G. 2022. MorphOMICs, a tool for mapping microglial morphology, reveals brain region- and sex-dependent phenotypes. Institute of Science and Technology Austria."},"page":"142"},{"article_processing_charge":"No","month":"08","day":"18","citation":{"chicago":"Michalska, Julia M, Julia Lyudchik, Philipp Velicky, Hana Korinkova, Jake Watson, Alban Cenameri, Christoph M Sommer, et al. “Uncovering Brain Tissue Architecture across Scales with Super-Resolution Light Microscopy.” BioRxiv. Cold Spring Harbor Laboratory, n.d. https://doi.org/10.1101/2022.08.17.504272.","short":"J.M. Michalska, J. Lyudchik, P. Velicky, H. Korinkova, J. Watson, A. Cenameri, C.M. Sommer, A. Venturino, K. Roessler, T. Czech, S. Siegert, G. Novarino, P.M. Jonas, J.G. Danzl, BioRxiv (n.d.).","mla":"Michalska, Julia M., et al. “Uncovering Brain Tissue Architecture across Scales with Super-Resolution Light Microscopy.” BioRxiv, Cold Spring Harbor Laboratory, doi:10.1101/2022.08.17.504272.","apa":"Michalska, J. M., Lyudchik, J., Velicky, P., Korinkova, H., Watson, J., Cenameri, A., … Danzl, J. G. (n.d.). Uncovering brain tissue architecture across scales with super-resolution light microscopy. bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2022.08.17.504272","ieee":"J. M. Michalska et al., “Uncovering brain tissue architecture across scales with super-resolution light microscopy,” bioRxiv. Cold Spring Harbor Laboratory.","ista":"Michalska JM, Lyudchik J, Velicky P, Korinkova H, Watson J, Cenameri A, Sommer CM, Venturino A, Roessler K, Czech T, Siegert S, Novarino G, Jonas PM, Danzl JG. Uncovering brain tissue architecture across scales with super-resolution light microscopy. bioRxiv, 10.1101/2022.08.17.504272.","ama":"Michalska JM, Lyudchik J, Velicky P, et al. Uncovering brain tissue architecture across scales with super-resolution light microscopy. bioRxiv. doi:10.1101/2022.08.17.504272"},"main_file_link":[{"url":"https://doi.org/10.1101/2022.08.17.504272","open_access":"1"}],"oa":1,"publication":"bioRxiv","date_published":"2022-08-18T00:00:00Z","doi":"10.1101/2022.08.17.504272","language":[{"iso":"eng"}],"type":"preprint","abstract":[{"text":"Mapping the complex and dense arrangement of cells and their connectivity in brain tissue demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels at visualizing specific molecules and individual cells but fails to provide tissue context. Here we developed Comprehensive Analysis of Tissues across Scales (CATS), a technology to densely map brain tissue architecture from millimeter regional to nanoscopic synaptic scales in diverse chemically fixed brain preparations, including rodent and human. CATS leverages fixation-compatible extracellular labeling and advanced optical readout, in particular stimulated-emission depletion and expansion microscopy, to comprehensively delineate cellular structures. It enables 3D-reconstructing single synapses and mapping synaptic connectivity by identification and tailored analysis of putative synaptic cleft regions. Applying CATS to the hippocampal mossy fiber circuitry, we demonstrate its power to reveal the system’s molecularly informed ultrastructure across spatial scales and assess local connectivity by reconstructing and quantifying the synaptic input and output structure of identified neurons.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"11950","year":"2022","department":[{"_id":"SaSi"},{"_id":"GaNo"},{"_id":"PeJo"},{"_id":"JoDa"}],"publisher":"Cold Spring Harbor Laboratory","title":"Uncovering brain tissue architecture across scales with super-resolution light microscopy","status":"public","publication_status":"submitted","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"12470"}]},"author":[{"id":"443DB6DE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3862-1235","first_name":"Julia M","last_name":"Michalska","full_name":"Michalska, Julia M"},{"first_name":"Julia","last_name":"Lyudchik","id":"46E28B80-F248-11E8-B48F-1D18A9856A87","full_name":"Lyudchik, Julia"},{"full_name":"Velicky, Philipp","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2340-7431","first_name":"Philipp","last_name":"Velicky"},{"full_name":"Korinkova, Hana","first_name":"Hana","last_name":"Korinkova","id":"ee3cb6ca-ec98-11ea-ae11-ff703e2254ed"},{"first_name":"Jake","last_name":"Watson","id":"63836096-4690-11EA-BD4E-32803DDC885E","orcid":"0000-0002-8698-3823","full_name":"Watson, Jake"},{"last_name":"Cenameri","first_name":"Alban","id":"9ac8f577-2357-11eb-997a-e566c5550886","full_name":"Cenameri, Alban"},{"full_name":"Sommer, Christoph M","last_name":"Sommer","first_name":"Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Venturino, Alessandro","last_name":"Venturino","first_name":"Alessandro","orcid":"0000-0003-2356-9403","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Roessler, Karl","first_name":"Karl","last_name":"Roessler"},{"first_name":"Thomas","last_name":"Czech","full_name":"Czech, Thomas"},{"full_name":"Siegert, Sandra","orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","last_name":"Siegert","first_name":"Sandra"},{"full_name":"Novarino, Gaia","first_name":"Gaia","last_name":"Novarino","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178"},{"full_name":"Jonas, Peter M","last_name":"Jonas","first_name":"Peter M","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","first_name":"Johann G","full_name":"Danzl, Johann G"}],"oa_version":"Preprint","date_created":"2022-08-24T08:24:52Z","date_updated":"2024-03-28T23:30:20Z"},{"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9009","intvolume":" 26","title":"Characteristics of intracellular propagation of mitochondrial BAX recruitment during apoptosis","status":"public","oa_version":"Submitted Version","type":"journal_article","issue":"2","abstract":[{"text":"Recent advancements in live cell imaging technologies have identified the phenomenon of intracellular propagation of late apoptotic events, such as cytochrome c release and caspase activation. The mechanism, prevalence, and speed of apoptosis propagation remain unclear. Additionally, no studies have demonstrated propagation of the pro-apoptotic protein, BAX. To evaluate the role of BAX in intracellular apoptotic propagation, we used high speed live-cell imaging to visualize fluorescently tagged-BAX recruitment to mitochondria in four immortalized cell lines. We show that propagation of mitochondrial BAX recruitment occurs in parallel to cytochrome c and SMAC/Diablo release and is affected by cellular morphology, such that cells with processes are more likely to exhibit propagation. The initiation of propagation events is most prevalent in the distal tips of processes, while the rate of propagation is influenced by the 2-dimensional width of the process. Propagation was rarely observed in the cell soma, which exhibited near synchronous recruitment of BAX. Propagation velocity is not affected by mitochondrial volume in segments of processes, but is negatively affected by mitochondrial density. There was no evidence of a propagating wave of increased levels of intracellular calcium ions. Alternatively, we did observe a uniform increase in superoxide build-up in cellular mitochondria, which was released as a propagating wave simultaneously with the propagating recruitment of BAX to the mitochondrial outer membrane.","lang":"eng"}],"citation":{"ama":"Grosser JA, Maes ME, Nickells RW. Characteristics of intracellular propagation of mitochondrial BAX recruitment during apoptosis. Apoptosis. 2021;26(2):132-145. doi:10.1007/s10495-020-01654-w","ieee":"J. A. Grosser, M. E. Maes, and R. W. Nickells, “Characteristics of intracellular propagation of mitochondrial BAX recruitment during apoptosis,” Apoptosis, vol. 26, no. 2. Springer Nature, pp. 132–145, 2021.","apa":"Grosser, J. A., Maes, M. E., & Nickells, R. W. (2021). Characteristics of intracellular propagation of mitochondrial BAX recruitment during apoptosis. Apoptosis. Springer Nature. https://doi.org/10.1007/s10495-020-01654-w","ista":"Grosser JA, Maes ME, Nickells RW. 2021. Characteristics of intracellular propagation of mitochondrial BAX recruitment during apoptosis. Apoptosis. 26(2), 132–145.","short":"J.A. Grosser, M.E. Maes, R.W. Nickells, Apoptosis 26 (2021) 132–145.","mla":"Grosser, Joshua A., et al. “Characteristics of Intracellular Propagation of Mitochondrial BAX Recruitment during Apoptosis.” Apoptosis, vol. 26, no. 2, Springer Nature, 2021, pp. 132–45, doi:10.1007/s10495-020-01654-w.","chicago":"Grosser, Joshua A., Margaret E Maes, and Robert W. Nickells. “Characteristics of Intracellular Propagation of Mitochondrial BAX Recruitment during Apoptosis.” Apoptosis. Springer Nature, 2021. https://doi.org/10.1007/s10495-020-01654-w."},"publication":"Apoptosis","page":"132-145","article_type":"original","date_published":"2021-02-01T00:00:00Z","scopus_import":"1","article_processing_charge":"No","day":"01","pmid":1,"acknowledgement":"This work was supported by National Institute of Health grants R01 EY030123, P30 EY016665, and T32 GM081061, an unrestricted research grant from Research to Prevent Blindness, Inc., and the Frederick A. Davis Endowment from the Department of Ophthalmology and Visual Sciences at the University of Wisconsin-Madison.","year":"2021","publisher":"Springer Nature","department":[{"_id":"SaSi"}],"publication_status":"published","author":[{"first_name":"Joshua A.","last_name":"Grosser","full_name":"Grosser, Joshua A."},{"full_name":"Maes, Margaret E","first_name":"Margaret E","last_name":"Maes","id":"3838F452-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9642-1085"},{"last_name":"Nickells","first_name":"Robert W.","full_name":"Nickells, Robert W."}],"volume":26,"date_updated":"2023-08-07T13:32:40Z","date_created":"2021-01-17T23:01:11Z","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082518/"}],"oa":1,"external_id":{"isi":["000606722600001"],"pmid":["33426618"]},"quality_controlled":"1","isi":1,"doi":"10.1007/s10495-020-01654-w","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1573-675X"],"issn":["1360-8185"]},"month":"02"},{"article_number":"109313","file_date_updated":"2021-07-19T13:32:17Z","ec_funded":1,"acknowledgement":"We thank the scientific service units at IST Austria, especially the IST bioimaging facility, the preclinical facility, and, specifically, Michael Schunn and Sonja Haslinger for excellent support; Plexxikon for the PLX food; the Csicsvari group for advice and equipment for in vivo recording; Jürgen Siegert for the light-entrainment design; Marco Benevento, Soledad Gonzalo Cogno, Pat King, and all Siegert group members for constant feedback on the project and manuscript; Lorena Pantano (PILM Bioinformatics Core) for assisting with sample-size determination for OD plasticity experiments; and Ana Morello from MIT for technical assistance with VEPs recordings. This research was supported by a DOC Fellowship from the Austrian Academy of Sciences at the Institute of Science and Technology Austria to R.S., from the European Union Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Actions program (grants 665385 to G.C.; 754411 to R.J.A.C.), the European Research Council (grant 715571 to S.S.), and the National Eye Institute of the National Institutes of Health under award numbers R01EY029245 (to M.F.B.) and R01EY023037 (diversity supplement to H.D.J-C.).","year":"2021","pmid":1,"publication_status":"published","publisher":"Elsevier","department":[{"_id":"SaSi"}],"author":[{"full_name":"Venturino, Alessandro","last_name":"Venturino","first_name":"Alessandro","orcid":"0000-0003-2356-9403","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Schulz","first_name":"Rouven","orcid":"0000-0001-5297-733X","id":"4C5E7B96-F248-11E8-B48F-1D18A9856A87","full_name":"Schulz, Rouven"},{"full_name":"De Jesús-Cortés, Héctor","first_name":"Héctor","last_name":"De Jesús-Cortés"},{"first_name":"Margaret E","last_name":"Maes","id":"3838F452-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9642-1085","full_name":"Maes, Margaret E"},{"full_name":"Nagy, Balint","id":"93C65ECC-A6F2-11E9-8DF9-9712E6697425","first_name":"Balint","last_name":"Nagy"},{"first_name":"Francis","last_name":"Reilly-Andújar","full_name":"Reilly-Andújar, Francis"},{"full_name":"Colombo, Gloria","last_name":"Colombo","first_name":"Gloria","orcid":"0000-0001-9434-8902","id":"3483CF6C-F248-11E8-B48F-1D18A9856A87"},{"id":"850B2E12-9CD4-11E9-837F-E719E6697425","orcid":"0000-0003-0002-1867","first_name":"Ryan J","last_name":"Cubero","full_name":"Cubero, Ryan J"},{"id":"3526230C-F248-11E8-B48F-1D18A9856A87","first_name":"Florianne E","last_name":"Schoot Uiterkamp","full_name":"Schoot Uiterkamp, Florianne E"},{"full_name":"Bear, Mark F.","first_name":"Mark F.","last_name":"Bear"},{"full_name":"Siegert, Sandra","first_name":"Sandra","last_name":"Siegert","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8635-0877"}],"related_material":{"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/the-twinkle-and-the-brain/"}]},"date_created":"2021-07-11T22:01:16Z","date_updated":"2023-08-10T14:09:39Z","volume":36,"month":"07","publication_identifier":{"eissn":["22111247"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"pmid":["34233180"],"isi":["000670188500004"]},"quality_controlled":"1","isi":1,"project":[{"name":"International IST Doctoral Program","call_identifier":"H2020","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020","_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411"},{"call_identifier":"H2020","name":"Microglia action towards neuronal circuit formation and function in health and disease","_id":"25D4A630-B435-11E9-9278-68D0E5697425","grant_number":"715571"}],"doi":"10.1016/j.celrep.2021.109313","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"language":[{"iso":"eng"}],"type":"journal_article","abstract":[{"text":"Perineuronal nets (PNNs), components of the extracellular matrix, preferentially coat parvalbumin-positive interneurons and constrain critical-period plasticity in the adult cerebral cortex. Current strategies to remove PNN are long-lasting, invasive, and trigger neuropsychiatric symptoms. Here, we apply repeated anesthetic ketamine as a method with minimal behavioral effect. We find that this paradigm strongly reduces PNN coating in the healthy adult brain and promotes juvenile-like plasticity. Microglia are critically involved in PNN loss because they engage with parvalbumin-positive neurons in their defined cortical layer. We identify external 60-Hz light-flickering entrainment to recapitulate microglia-mediated PNN removal. Importantly, 40-Hz frequency, which is known to remove amyloid plaques, does not induce PNN loss, suggesting microglia might functionally tune to distinct brain frequencies. Thus, our 60-Hz light-entrainment strategy provides an alternative form of PNN intervention in the healthy adult brain.","lang":"eng"}],"issue":"1","_id":"9642","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","ddc":["570"],"title":"Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain","status":"public","intvolume":" 36","file":[{"date_created":"2021-07-19T13:32:17Z","date_updated":"2021-07-19T13:32:17Z","success":1,"checksum":"f056255f6d01fd9a86b5387635928173","file_id":"9693","relation":"main_file","creator":"cziletti","content_type":"application/pdf","file_size":56388540,"file_name":"2021_CellReports_Venturino.pdf","access_level":"open_access"}],"oa_version":"Published Version","scopus_import":"1","day":"06","has_accepted_license":"1","article_processing_charge":"No","publication":"Cell Reports","citation":{"short":"A. Venturino, R. Schulz, H. De Jesús-Cortés, M.E. Maes, B. Nagy, F. Reilly-Andújar, G. Colombo, R.J. Cubero, F.E. Schoot Uiterkamp, M.F. Bear, S. Siegert, Cell Reports 36 (2021).","mla":"Venturino, Alessandro, et al. “Microglia Enable Mature Perineuronal Nets Disassembly upon Anesthetic Ketamine Exposure or 60-Hz Light Entrainment in the Healthy Brain.” Cell Reports, vol. 36, no. 1, 109313, Elsevier, 2021, doi:10.1016/j.celrep.2021.109313.","chicago":"Venturino, Alessandro, Rouven Schulz, Héctor De Jesús-Cortés, Margaret E Maes, Balint Nagy, Francis Reilly-Andújar, Gloria Colombo, et al. “Microglia Enable Mature Perineuronal Nets Disassembly upon Anesthetic Ketamine Exposure or 60-Hz Light Entrainment in the Healthy Brain.” Cell Reports. Elsevier, 2021. https://doi.org/10.1016/j.celrep.2021.109313.","ama":"Venturino A, Schulz R, De Jesús-Cortés H, et al. Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain. Cell Reports. 2021;36(1). doi:10.1016/j.celrep.2021.109313","apa":"Venturino, A., Schulz, R., De Jesús-Cortés, H., Maes, M. E., Nagy, B., Reilly-Andújar, F., … Siegert, S. (2021). Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain. Cell Reports. Elsevier. https://doi.org/10.1016/j.celrep.2021.109313","ieee":"A. Venturino et al., “Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain,” Cell Reports, vol. 36, no. 1. Elsevier, 2021.","ista":"Venturino A, Schulz R, De Jesús-Cortés H, Maes ME, Nagy B, Reilly-Andújar F, Colombo G, Cubero RJ, Schoot Uiterkamp FE, Bear MF, Siegert S. 2021. Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain. Cell Reports. 36(1), 109313."},"article_type":"original","date_published":"2021-07-06T00:00:00Z"},{"type":"journal_article","abstract":[{"text":"The important roles of mitochondrial function and dysfunction in the process of neurodegeneration are widely acknowledged. Retinal ganglion cells (RGCs) appear to be a highly vulnerable neuronal cell type in the central nervous system with respect to mitochondrial dysfunction but the actual reasons for this are still incompletely understood. These cells have a unique circumstance where unmyelinated axons must bend nearly 90° to exit the eye and then cross a translaminar pressure gradient before becoming myelinated in the optic nerve. This region, the optic nerve head, contains some of the highest density of mitochondria present in these cells. Glaucoma represents a perfect storm of events occurring at this location, with a combination of changes in the translaminar pressure gradient and reassignment of the metabolic support functions of supporting glia, which appears to apply increased metabolic stress to the RGC axons leading to a failure of axonal transport mechanisms. However, RGCs themselves are also extremely sensitive to genetic mutations, particularly in genes affecting mitochondrial dynamics and mitochondrial clearance. These mutations, which systemically affect the mitochondria in every cell, often lead to an optic neuropathy as the sole pathologic defect in affected patients. This review summarizes knowledge of mitochondrial structure and function, the known energy demands of neurons in general, and places these in the context of normal and pathological characteristics of mitochondria attributed to RGCs. ","lang":"eng"}],"issue":"7","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9761","title":"The influence of mitochondrial dynamics and function on retinal ganglion cell susceptibility in optic nerve disease","ddc":["570"],"status":"public","intvolume":" 10","oa_version":"Published Version","file":[{"creator":"cziletti","file_size":4555611,"content_type":"application/pdf","file_name":"2021_Cells_Muench.pdf","access_level":"open_access","date_created":"2021-08-04T14:01:30Z","date_updated":"2021-08-04T14:01:30Z","success":1,"checksum":"e0497ce5c77fa3b65a538c7d6e0f6c66","file_id":"9768","relation":"main_file"}],"scopus_import":"1","day":"25","article_processing_charge":"Yes","has_accepted_license":"1","publication":"Cells","citation":{"apa":"Muench, N. A., Patel, S., Maes, M. E., Donahue, R. J., Ikeda, A., & Nickells, R. W. (2021). The influence of mitochondrial dynamics and function on retinal ganglion cell susceptibility in optic nerve disease. Cells. MDPI. https://doi.org/10.3390/cells10071593","ieee":"N. A. Muench, S. Patel, M. E. Maes, R. J. Donahue, A. Ikeda, and R. W. Nickells, “The influence of mitochondrial dynamics and function on retinal ganglion cell susceptibility in optic nerve disease,” Cells, vol. 10, no. 7. MDPI, 2021.","ista":"Muench NA, Patel S, Maes ME, Donahue RJ, Ikeda A, Nickells RW. 2021. The influence of mitochondrial dynamics and function on retinal ganglion cell susceptibility in optic nerve disease. Cells. 10(7), 1593.","ama":"Muench NA, Patel S, Maes ME, Donahue RJ, Ikeda A, Nickells RW. The influence of mitochondrial dynamics and function on retinal ganglion cell susceptibility in optic nerve disease. Cells. 2021;10(7). doi:10.3390/cells10071593","chicago":"Muench, Nicole A., Sonia Patel, Margaret E Maes, Ryan J. Donahue, Akihiro Ikeda, and Robert W. Nickells. “The Influence of Mitochondrial Dynamics and Function on Retinal Ganglion Cell Susceptibility in Optic Nerve Disease.” Cells. MDPI, 2021. https://doi.org/10.3390/cells10071593.","short":"N.A. Muench, S. Patel, M.E. Maes, R.J. Donahue, A. Ikeda, R.W. Nickells, Cells 10 (2021).","mla":"Muench, Nicole A., et al. “The Influence of Mitochondrial Dynamics and Function on Retinal Ganglion Cell Susceptibility in Optic Nerve Disease.” Cells, vol. 10, no. 7, 1593, MDPI, 2021, doi:10.3390/cells10071593."},"article_type":"original","date_published":"2021-06-25T00:00:00Z","article_number":"1593","file_date_updated":"2021-08-04T14:01:30Z","year":"2021","acknowledgement":"The authors are grateful to Kazuya Oikawa and Gillian McLellan for generously sharing some of their data for this review, and to Janis Eells for helpful comments on the manuscript.","pmid":1,"publication_status":"published","publisher":"MDPI","department":[{"_id":"SaSi"}],"author":[{"full_name":"Muench, Nicole A.","first_name":"Nicole A.","last_name":"Muench"},{"first_name":"Sonia","last_name":"Patel","full_name":"Patel, Sonia"},{"first_name":"Margaret E","last_name":"Maes","id":"3838F452-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9642-1085","full_name":"Maes, Margaret E"},{"first_name":"Ryan J.","last_name":"Donahue","full_name":"Donahue, Ryan J."},{"last_name":"Ikeda","first_name":"Akihiro","full_name":"Ikeda, Akihiro"},{"first_name":"Robert W.","last_name":"Nickells","full_name":"Nickells, Robert W."}],"date_updated":"2023-08-10T14:14:53Z","date_created":"2021-08-01T22:01:22Z","volume":10,"month":"06","publication_identifier":{"eissn":["20734409"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"isi":["000678193300001"],"pmid":["34201955"]},"quality_controlled":"1","isi":1,"doi":"10.3390/cells10071593","language":[{"iso":"eng"}]},{"article_processing_charge":"Yes","has_accepted_license":"1","day":"10","genbank":["GSE178867"],"scopus_import":"1","date_published":"2021-08-10T00:00:00Z","article_type":"original","citation":{"short":"A. Raso, E. Dirkx, V. Sampaio-Pinto, H. el Azzouzi, R.J. Cubero, D.W. Sorensen, L. Ottaviani, S. Olieslagers, M.M. Huibers, R. de Weger, S. Siddiqi, S. Moimas, C. Torrini, L. Zentillin, L. Braga, D.S. Nascimento, P.A. da Costa Martins, J.H. van Berlo, S. Zacchigna, M. Giacca, L.J. De Windt, Nature Communications 12 (2021).","mla":"Raso, Andrea, et al. “A MicroRNA Program Regulates the Balance between Cardiomyocyte Hyperplasia and Hypertrophy and Stimulates Cardiac Regeneration.” Nature Communications, vol. 12, 4808, Springer Nature, 2021, doi:10.1038/s41467-021-25211-4.","chicago":"Raso, Andrea, Ellen Dirkx, Vasco Sampaio-Pinto, Hamid el Azzouzi, Ryan J Cubero, Daniel W. Sorensen, Lara Ottaviani, et al. “A MicroRNA Program Regulates the Balance between Cardiomyocyte Hyperplasia and Hypertrophy and Stimulates Cardiac Regeneration.” Nature Communications. Springer Nature, 2021. https://doi.org/10.1038/s41467-021-25211-4.","ama":"Raso A, Dirkx E, Sampaio-Pinto V, et al. A microRNA program regulates the balance between cardiomyocyte hyperplasia and hypertrophy and stimulates cardiac regeneration. Nature Communications. 2021;12. doi:10.1038/s41467-021-25211-4","ieee":"A. Raso et al., “A microRNA program regulates the balance between cardiomyocyte hyperplasia and hypertrophy and stimulates cardiac regeneration,” Nature Communications, vol. 12. Springer Nature, 2021.","apa":"Raso, A., Dirkx, E., Sampaio-Pinto, V., el Azzouzi, H., Cubero, R. J., Sorensen, D. W., … De Windt, L. J. (2021). A microRNA program regulates the balance between cardiomyocyte hyperplasia and hypertrophy and stimulates cardiac regeneration. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-021-25211-4","ista":"Raso A, Dirkx E, Sampaio-Pinto V, el Azzouzi H, Cubero RJ, Sorensen DW, Ottaviani L, Olieslagers S, Huibers MM, de Weger R, Siddiqi S, Moimas S, Torrini C, Zentillin L, Braga L, Nascimento DS, da Costa Martins PA, van Berlo JH, Zacchigna S, Giacca M, De Windt LJ. 2021. A microRNA program regulates the balance between cardiomyocyte hyperplasia and hypertrophy and stimulates cardiac regeneration. Nature Communications. 12, 4808."},"publication":"Nature Communications","abstract":[{"lang":"eng","text":"Myocardial regeneration is restricted to early postnatal life, when mammalian cardiomyocytes still retain the ability to proliferate. The molecular cues that induce cell cycle arrest of neonatal cardiomyocytes towards terminally differentiated adult heart muscle cells remain obscure. Here we report that the miR-106b~25 cluster is higher expressed in the early postnatal myocardium and decreases in expression towards adulthood, especially under conditions of overload, and orchestrates the transition of cardiomyocyte hyperplasia towards cell cycle arrest and hypertrophy by virtue of its targetome. In line, gene delivery of miR-106b~25 to the mouse heart provokes cardiomyocyte proliferation by targeting a network of negative cell cycle regulators including E2f5, Cdkn1c, Ccne1 and Wee1. Conversely, gene-targeted miR-106b~25 null mice display spontaneous hypertrophic remodeling and exaggerated remodeling to overload by derepression of the prohypertrophic transcription factors Hand2 and Mef2d. Taking advantage of the regulatory function of miR-106b~25 on cardiomyocyte hyperplasia and hypertrophy, viral gene delivery of miR-106b~25 provokes nearly complete regeneration of the adult myocardium after ischemic injury. Our data demonstrate that exploitation of conserved molecular programs can enhance the regenerative capacity of the injured heart."}],"type":"journal_article","oa_version":"Published Version","file":[{"creator":"asandaue","content_type":"application/pdf","file_size":4364333,"access_level":"open_access","file_name":"2021_NatureCommunications_Raso.pdf","success":1,"checksum":"48d8562e8229e4282f3f354b329722c5","date_created":"2021-08-10T12:29:59Z","date_updated":"2021-08-10T12:29:59Z","file_id":"9876","relation":"main_file"}],"intvolume":" 12","title":"A microRNA program regulates the balance between cardiomyocyte hyperplasia and hypertrophy and stimulates cardiac regeneration","ddc":["610","570"],"status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9874","publication_identifier":{"eissn":["2041-1723"]},"month":"08","language":[{"iso":"eng"}],"doi":"10.1038/s41467-021-25211-4","quality_controlled":"1","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"external_id":{"pmid":["34376683"],"isi":["000683910200042"]},"file_date_updated":"2021-08-10T12:29:59Z","article_number":"4808","volume":12,"date_updated":"2023-08-11T10:27:03Z","date_created":"2021-08-10T11:49:20Z","related_material":{"link":[{"url":"https://doi.org/10.1038/s41467-022-32785-0","relation":"erratum"}]},"author":[{"first_name":"Andrea","last_name":"Raso","full_name":"Raso, Andrea"},{"last_name":"Dirkx","first_name":"Ellen","full_name":"Dirkx, Ellen"},{"full_name":"Sampaio-Pinto, Vasco","last_name":"Sampaio-Pinto","first_name":"Vasco"},{"last_name":"el Azzouzi","first_name":"Hamid","full_name":"el Azzouzi, Hamid"},{"last_name":"Cubero","first_name":"Ryan J","orcid":"0000-0003-0002-1867","id":"850B2E12-9CD4-11E9-837F-E719E6697425","full_name":"Cubero, Ryan J"},{"full_name":"Sorensen, Daniel W.","first_name":"Daniel W.","last_name":"Sorensen"},{"full_name":"Ottaviani, Lara","last_name":"Ottaviani","first_name":"Lara"},{"last_name":"Olieslagers","first_name":"Servé","full_name":"Olieslagers, Servé"},{"first_name":"Manon M.","last_name":"Huibers","full_name":"Huibers, Manon M."},{"full_name":"de Weger, Roel","first_name":"Roel","last_name":"de Weger"},{"first_name":"Sailay","last_name":"Siddiqi","full_name":"Siddiqi, Sailay"},{"full_name":"Moimas, Silvia","first_name":"Silvia","last_name":"Moimas"},{"first_name":"Consuelo","last_name":"Torrini","full_name":"Torrini, Consuelo"},{"full_name":"Zentillin, Lorena","last_name":"Zentillin","first_name":"Lorena"},{"full_name":"Braga, Luca","last_name":"Braga","first_name":"Luca"},{"last_name":"Nascimento","first_name":"Diana S.","full_name":"Nascimento, Diana S."},{"last_name":"da Costa Martins","first_name":"Paula A.","full_name":"da Costa Martins, Paula A."},{"full_name":"van Berlo, Jop H.","last_name":"van Berlo","first_name":"Jop H."},{"full_name":"Zacchigna, Serena","last_name":"Zacchigna","first_name":"Serena"},{"full_name":"Giacca, Mauro","first_name":"Mauro","last_name":"Giacca"},{"first_name":"Leon J.","last_name":"De Windt","full_name":"De Windt, Leon J."}],"publisher":"Springer Nature","department":[{"_id":"SaSi"}],"publication_status":"published","pmid":1,"year":"2021","acknowledgement":"E.D. is supported by a VENI award 916-150-16 from the Netherlands Organization for Health Research and Development (ZonMW), an EMBO Long-term Fellowship (EMBO ALTF 848-2013) and a FP7 Marie Curie Intra-European Fellowship (Project number 627539). V.S.P. was funded by a fellowship from the FCT/ Ministério da Ciência, Tecnologia e Inovação SFRH/BD/111799/2015. P.D.C.M. is an Established Investigator of the Dutch Heart Foundation. L.D.W. acknowledges support from the Dutch CardioVascular Alliance (ARENA-PRIME). L.D.W. was further supported by grant 311549 from the European Research Council (ERC), a VICI award 918-156-47 from the Dutch Research Council and Marie Sklodowska-Curie grant agreement no. 813716 (TRAIN-HEART)."},{"type":"journal_article","abstract":[{"lang":"eng","text":"Inhibition or targeted deletion of histone deacetylase 3 (HDAC3) is neuroprotective in a variety neurodegenerative conditions, including retinal ganglion cells (RGCs) after acute optic nerve damage. Consistent with this, induced HDAC3 expression in cultured cells shows selective toxicity to neurons. Despite an established role for HDAC3 in neuronal pathology, little is known regarding the mechanism of this pathology."}],"issue":"10","ddc":["570"],"status":"public","title":"Increased susceptibility and intrinsic apoptotic signaling in neurons by induced HDAC3 expression","intvolume":" 62","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"10000","oa_version":"Published Version","file":[{"access_level":"open_access","file_name":"2021_IOVS_Schmitt.pdf","creator":"dernst","file_size":19707796,"content_type":"application/pdf","file_id":"11369","relation":"main_file","success":1,"checksum":"c430967746f653aa1ae84ee617f62b73","date_created":"2022-05-13T07:40:15Z","date_updated":"2022-05-13T07:40:15Z"}],"scopus_import":"1","day":"16","has_accepted_license":"1","article_processing_charge":"Yes","article_type":"original","publication":"Investigative Ophthalmology and Visual Science","citation":{"ieee":"H. M. Schmitt et al., “Increased susceptibility and intrinsic apoptotic signaling in neurons by induced HDAC3 expression,” Investigative Ophthalmology and Visual Science, vol. 62, no. 10. Association for Research in Vision and Ophthalmology, 2021.","apa":"Schmitt, H. M., Fehrman, R. L., Maes, M. E., Yang, H., Guo, L. W., Schlamp, C. L., … Nickells, R. W. (2021). Increased susceptibility and intrinsic apoptotic signaling in neurons by induced HDAC3 expression. Investigative Ophthalmology and Visual Science. Association for Research in Vision and Ophthalmology. https://doi.org/10.1167/IOVS.62.10.14","ista":"Schmitt HM, Fehrman RL, Maes ME, Yang H, Guo LW, Schlamp CL, Pelzel HR, Nickells RW. 2021. Increased susceptibility and intrinsic apoptotic signaling in neurons by induced HDAC3 expression. Investigative Ophthalmology and Visual Science. 62(10), 14.","ama":"Schmitt HM, Fehrman RL, Maes ME, et al. Increased susceptibility and intrinsic apoptotic signaling in neurons by induced HDAC3 expression. Investigative Ophthalmology and Visual Science. 2021;62(10). doi:10.1167/IOVS.62.10.14","chicago":"Schmitt, Heather M., Rachel L. Fehrman, Margaret E Maes, Huan Yang, Lian Wang Guo, Cassandra L. Schlamp, Heather R. Pelzel, and Robert W. Nickells. “Increased Susceptibility and Intrinsic Apoptotic Signaling in Neurons by Induced HDAC3 Expression.” Investigative Ophthalmology and Visual Science. Association for Research in Vision and Ophthalmology, 2021. https://doi.org/10.1167/IOVS.62.10.14.","short":"H.M. Schmitt, R.L. Fehrman, M.E. Maes, H. Yang, L.W. Guo, C.L. Schlamp, H.R. Pelzel, R.W. Nickells, Investigative Ophthalmology and Visual Science 62 (2021).","mla":"Schmitt, Heather M., et al. “Increased Susceptibility and Intrinsic Apoptotic Signaling in Neurons by Induced HDAC3 Expression.” Investigative Ophthalmology and Visual Science, vol. 62, no. 10, 14, Association for Research in Vision and Ophthalmology, 2021, doi:10.1167/IOVS.62.10.14."},"date_published":"2021-08-16T00:00:00Z","article_number":"14","file_date_updated":"2022-05-13T07:40:15Z","publication_status":"published","department":[{"_id":"SaSi"}],"publisher":"Association for Research in Vision and Ophthalmology","acknowledgement":"The authors thank Joel Dietz for maintaining the mice used in this study, Satoshi Kinoshita and the Translational Research Initiative in Pathology Laboratory at the University of Wisconsin-Madison for cutting retinal sections analyzed in this study, and Mark Banghart for statistical review of the data analysis. Supported by National Eye Institute Grants R01 EY012223 (RWN), R01 EY030123 (RWN), R01 EY029809 (LWG), R01 EY029809 (LWG) and a Vision Research CORE grant P30 EY016665, NRSA grant T32 GM081061, by an unrestricted research grant from Research to Prevent Blindness, Inc., and by a University of Wisconsin-Madison Vilas Life Cycle award and the Frederick A. Davis Research Chair (RWN). ","year":"2021","pmid":1,"date_created":"2021-09-12T22:01:23Z","date_updated":"2023-08-14T06:35:17Z","volume":62,"author":[{"last_name":"Schmitt","first_name":"Heather M.","full_name":"Schmitt, Heather M."},{"first_name":"Rachel L.","last_name":"Fehrman","full_name":"Fehrman, Rachel L."},{"id":"3838F452-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9642-1085","first_name":"Margaret E","last_name":"Maes","full_name":"Maes, Margaret E"},{"full_name":"Yang, Huan","last_name":"Yang","first_name":"Huan"},{"last_name":"Guo","first_name":"Lian Wang","full_name":"Guo, Lian Wang"},{"full_name":"Schlamp, Cassandra L.","last_name":"Schlamp","first_name":"Cassandra L."},{"first_name":"Heather R.","last_name":"Pelzel","full_name":"Pelzel, Heather R."},{"full_name":"Nickells, Robert W.","last_name":"Nickells","first_name":"Robert W."}],"month":"08","publication_identifier":{"eissn":["1552-5783"],"issn":["0146-0404"]},"quality_controlled":"1","isi":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"external_id":{"isi":["000695230000014"],"pmid":["34398198"]},"language":[{"iso":"eng"}],"doi":"10.1167/IOVS.62.10.14"},{"ec_funded":1,"file_date_updated":"2022-01-24T07:43:09Z","volume":23,"date_updated":"2023-11-16T13:12:03Z","date_created":"2022-01-23T23:01:28Z","author":[{"id":"3838F452-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9642-1085","first_name":"Margaret E","last_name":"Maes","full_name":"Maes, Margaret E"},{"full_name":"Wögenstein, Gabriele M.","last_name":"Wögenstein","first_name":"Gabriele M."},{"full_name":"Colombo, Gloria","orcid":"0000-0001-9434-8902","id":"3483CF6C-F248-11E8-B48F-1D18A9856A87","last_name":"Colombo","first_name":"Gloria"},{"full_name":"Casado Polanco, Raquel","first_name":"Raquel","last_name":"Casado Polanco","id":"15240fc1-dbcd-11ea-9d1d-ac5a786425fd","orcid":"0000-0001-8293-4568"},{"id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8635-0877","first_name":"Sandra","last_name":"Siegert","full_name":"Siegert, Sandra"}],"department":[{"_id":"SaSi"},{"_id":"SiHi"}],"publisher":"Elsevier","publication_status":"published","acknowledgement":"This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 715571). The research was supported by the Scientific Service Units (SSU) of IST Austria through resources provided by the Bioimaging Facility, the Life Science Facility, and the Pre-Clinical Facility, namely Sonja Haslinger and Michael Schunn for their animal colony management and support. We would also like to thank Chakrabarty Lab for sharing the plasmids for AAV2/6 production. Finally, we would like to thank the Siegert team members for discussion about the manuscript.","year":"2021","publication_identifier":{"eissn":["2329-0501"]},"month":"12","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"doi":"10.1016/j.omtm.2021.09.006","project":[{"call_identifier":"H2020","name":"Microglia action towards neuronal circuit formation and function in health and disease","grant_number":"715571","_id":"25D4A630-B435-11E9-9278-68D0E5697425"}],"quality_controlled":"1","isi":1,"external_id":{"isi":["000748748500019"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"abstract":[{"lang":"eng","text":"Adeno-associated viruses (AAVs) are widely used to deliver genetic material in vivo to distinct cell types such as neurons or glial cells, allowing for targeted manipulation. Transduction of microglia is mostly excluded from this strategy, likely due to the cells’ heterogeneous state upon environmental changes, which makes AAV design challenging. Here, we established the retina as a model system for microglial AAV validation and optimization. First, we show that AAV2/6 transduced microglia in both synaptic layers, where layer preference corresponds to the intravitreal or subretinal delivery method. Surprisingly, we observed significantly enhanced microglial transduction during photoreceptor degeneration. Thus, we modified the AAV6 capsid to reduce heparin binding by introducing four point mutations (K531E, R576Q, K493S, and K459S), resulting in increased microglial transduction in the outer plexiform layer. Finally, to improve microglial-specific transduction, we validated a Cre-dependent transgene delivery cassette for use in combination with the Cx3cr1CreERT2 mouse line. Together, our results provide a foundation for future studies optimizing AAV-mediated microglia transduction and highlight that environmental conditions influence microglial transduction efficiency.\r\n"}],"type":"journal_article","file":[{"creator":"cchlebak","file_size":4794147,"content_type":"application/pdf","access_level":"open_access","file_name":"2021_MolTherMethodsClinDev_Maes.pdf","success":1,"checksum":"77dc540e8011c5475031bdf6ccef20a6","date_updated":"2022-01-24T07:43:09Z","date_created":"2022-01-24T07:43:09Z","file_id":"10657","relation":"main_file"}],"oa_version":"Published Version","intvolume":" 23","ddc":["570"],"title":"Optimizing AAV2/6 microglial targeting identified enhanced efficiency in the photoreceptor degenerative environment","status":"public","_id":"10655","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"Yes","has_accepted_license":"1","day":"10","scopus_import":"1","date_published":"2021-12-10T00:00:00Z","page":"210-224","article_type":"original","citation":{"chicago":"Maes, Margaret E, Gabriele M. Wögenstein, Gloria Colombo, Raquel Casado Polanco, and Sandra Siegert. “Optimizing AAV2/6 Microglial Targeting Identified Enhanced Efficiency in the Photoreceptor Degenerative Environment.” Molecular Therapy - Methods and Clinical Development. Elsevier, 2021. https://doi.org/10.1016/j.omtm.2021.09.006.","short":"M.E. Maes, G.M. Wögenstein, G. Colombo, R. Casado Polanco, S. Siegert, Molecular Therapy - Methods and Clinical Development 23 (2021) 210–224.","mla":"Maes, Margaret E., et al. “Optimizing AAV2/6 Microglial Targeting Identified Enhanced Efficiency in the Photoreceptor Degenerative Environment.” Molecular Therapy - Methods and Clinical Development, vol. 23, Elsevier, 2021, pp. 210–24, doi:10.1016/j.omtm.2021.09.006.","ieee":"M. E. Maes, G. M. Wögenstein, G. Colombo, R. Casado Polanco, and S. Siegert, “Optimizing AAV2/6 microglial targeting identified enhanced efficiency in the photoreceptor degenerative environment,” Molecular Therapy - Methods and Clinical Development, vol. 23. Elsevier, pp. 210–224, 2021.","apa":"Maes, M. E., Wögenstein, G. M., Colombo, G., Casado Polanco, R., & Siegert, S. (2021). Optimizing AAV2/6 microglial targeting identified enhanced efficiency in the photoreceptor degenerative environment. Molecular Therapy - Methods and Clinical Development. Elsevier. https://doi.org/10.1016/j.omtm.2021.09.006","ista":"Maes ME, Wögenstein GM, Colombo G, Casado Polanco R, Siegert S. 2021. Optimizing AAV2/6 microglial targeting identified enhanced efficiency in the photoreceptor degenerative environment. Molecular Therapy - Methods and Clinical Development. 23, 210–224.","ama":"Maes ME, Wögenstein GM, Colombo G, Casado Polanco R, Siegert S. Optimizing AAV2/6 microglial targeting identified enhanced efficiency in the photoreceptor degenerative environment. Molecular Therapy - Methods and Clinical Development. 2021;23:210-224. doi:10.1016/j.omtm.2021.09.006"},"publication":"Molecular Therapy - Methods and Clinical Development"},{"type":"journal_article","abstract":[{"text":"Enzymatic digestion of the extracellular matrix with chondroitinase-ABC reinstates juvenile-like plasticity in the adult cortex as it also disassembles the perineuronal nets (PNNs). The disadvantage of the enzyme is that it must be applied intracerebrally and it degrades the ECM for several weeks. Here, we provide two minimally invasive and transient protocols for microglia-enabled PNN disassembly in mouse cortex: repeated treatment with ketamine-xylazine-acepromazine (KXA) anesthesia and 60-Hz light entrainment. We also discuss how to analyze PNNs within microglial endosomes-lysosomes. For complete details on the use and execution of this protocol, please refer to Venturino et al. (2021).","lang":"eng"}],"issue":"4","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"10565","status":"public","ddc":["573"],"title":"Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain","intvolume":" 2","oa_version":"Published Version","file":[{"file_name":"2021_STARProt_Venturino.pdf","access_level":"open_access","creator":"cchlebak","content_type":"application/pdf","file_size":6207060,"file_id":"10570","relation":"main_file","date_updated":"2021-12-20T08:58:40Z","date_created":"2021-12-20T08:58:40Z","success":1,"checksum":"9ea2501056c5df99e84726b845e9b976"}],"scopus_import":"1","day":"17","article_processing_charge":"Yes","has_accepted_license":"1","publication":"STAR Protocols","citation":{"mla":"Venturino, Alessandro, and Sandra Siegert. “Minimally Invasive Protocols and Quantification for Microglia-Mediated Perineuronal Net Disassembly in Mouse Brain.” STAR Protocols, vol. 2, no. 4, 101012, Elsevier ; Cell Press, 2021, doi:10.1016/j.xpro.2021.101012.","short":"A. Venturino, S. Siegert, STAR Protocols 2 (2021).","chicago":"Venturino, Alessandro, and Sandra Siegert. “Minimally Invasive Protocols and Quantification for Microglia-Mediated Perineuronal Net Disassembly in Mouse Brain.” STAR Protocols. Elsevier ; Cell Press, 2021. https://doi.org/10.1016/j.xpro.2021.101012.","ama":"Venturino A, Siegert S. Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain. STAR Protocols. 2021;2(4). doi:10.1016/j.xpro.2021.101012","ista":"Venturino A, Siegert S. 2021. Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain. STAR Protocols. 2(4), 101012.","ieee":"A. Venturino and S. Siegert, “Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain,” STAR Protocols, vol. 2, no. 4. Elsevier ; Cell Press, 2021.","apa":"Venturino, A., & Siegert, S. (2021). Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain. STAR Protocols. Elsevier ; Cell Press. https://doi.org/10.1016/j.xpro.2021.101012"},"article_type":"original","date_published":"2021-12-17T00:00:00Z","article_number":"101012","file_date_updated":"2021-12-20T08:58:40Z","ec_funded":1,"year":"2021","acknowledgement":"This research was supported by the European Research Council (grant 715571 to S.S.). We thank Rouven Schulz, Michael Schunn, Claudia Gold, Gabriel Krens, Sarah Gorkiewicz, Margaret Maes, Jürgen Siegert, Marco Benevento, and Sara Oakeley for comments on the manuscript and the IST Austria Bioimaging Facility for the technical support.","publication_status":"published","department":[{"_id":"SaSi"}],"publisher":"Elsevier ; Cell Press","author":[{"orcid":"0000-0003-2356-9403","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","last_name":"Venturino","first_name":"Alessandro","full_name":"Venturino, Alessandro"},{"id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8635-0877","first_name":"Sandra","last_name":"Siegert","full_name":"Siegert, Sandra"}],"date_created":"2021-12-19T23:01:32Z","date_updated":"2023-11-16T13:11:04Z","volume":2,"month":"12","publication_identifier":{"eissn":["2666-1667"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"quality_controlled":"1","project":[{"name":"Microglia action towards neuronal circuit formation and function in health and disease","call_identifier":"H2020","grant_number":"715571","_id":"25D4A630-B435-11E9-9278-68D0E5697425"}],"doi":"10.1016/j.xpro.2021.101012","acknowledged_ssus":[{"_id":"Bio"}],"language":[{"iso":"eng"}]},{"date_published":"2020-02-01T00:00:00Z","article_type":"original","page":"1070–1084","publication":"Molecular Neurobiology","citation":{"mla":"Donahue, RJ, et al. “BAX-Depleted Retinal Ganglion Cells Survive and Become Quiescent Following Optic Nerve Damage.” Molecular Neurobiology, vol. 57, no. 2, Springer Nature, 2020, pp. 1070–1084, doi:10.1007/s12035-019-01783-7.","short":"R. Donahue, M.E. Maes, J. Grosser, R. Nickells, Molecular Neurobiology 57 (2020) 1070–1084.","chicago":"Donahue, RJ, Margaret E Maes, JA Grosser, and RW Nickells. “BAX-Depleted Retinal Ganglion Cells Survive and Become Quiescent Following Optic Nerve Damage.” Molecular Neurobiology. Springer Nature, 2020. https://doi.org/10.1007/s12035-019-01783-7.","ama":"Donahue R, Maes ME, Grosser J, Nickells R. BAX-depleted retinal ganglion cells survive and become quiescent following optic nerve damage. Molecular Neurobiology. 2020;57(2):1070–1084. doi:10.1007/s12035-019-01783-7","ista":"Donahue R, Maes ME, Grosser J, Nickells R. 2020. BAX-depleted retinal ganglion cells survive and become quiescent following optic nerve damage. Molecular Neurobiology. 57(2), 1070–1084.","ieee":"R. Donahue, M. E. Maes, J. Grosser, and R. Nickells, “BAX-depleted retinal ganglion cells survive and become quiescent following optic nerve damage,” Molecular Neurobiology, vol. 57, no. 2. Springer Nature, pp. 1070–1084, 2020.","apa":"Donahue, R., Maes, M. E., Grosser, J., & Nickells, R. (2020). BAX-depleted retinal ganglion cells survive and become quiescent following optic nerve damage. Molecular Neurobiology. Springer Nature. https://doi.org/10.1007/s12035-019-01783-7"},"day":"01","article_processing_charge":"No","scopus_import":"1","oa_version":"Submitted Version","status":"public","title":"BAX-depleted retinal ganglion cells survive and become quiescent following optic nerve damage","intvolume":" 57","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"7033","abstract":[{"text":"Removal of the Bax gene from mice completely protects the somas of retinal ganglion cells (RGCs) from apoptosis following optic nerve injury. This makes BAX a promising therapeutic target to prevent neurodegeneration. In this study, Bax+/− mice were used to test the hypothesis that lowering the quantity of BAX in RGCs would delay apoptosis following optic nerve injury. RGCs were damaged by performing optic nerve crush (ONC) and then immunostaining for phospho-cJUN, and quantitative PCR were used to monitor the status of the BAX activation mechanism in the months following injury. The apoptotic susceptibility of injured cells was directly tested by virally introducing GFP-BAX into Bax−/− RGCs after injury. The competency of quiescent RGCs to reactivate their BAX activation mechanism was tested by intravitreal injection of the JNK pathway agonist, anisomycin. Twenty-four weeks after ONC, Bax+/− mice had significantly less cell loss in their RGC layer than Bax+/+ mice 3 weeks after ONC. Bax+/− and Bax+/+ RGCs exhibited similar patterns of nuclear phospho-cJUN accumulation immediately after ONC, which persisted in Bax+/− RGCs for up to 7 weeks before abating. The transcriptional activation of BAX-activating genes was similar in Bax+/− and Bax+/+ RGCs following ONC. Intriguingly, cells deactivated their BAX activation mechanism between 7 and 12 weeks after crush. Introduction of GFP-BAX into Bax−/− cells at 4 weeks after ONC showed that these cells had a nearly normal capacity to activate this protein, but this capacity was lost 8 weeks after crush. Collectively, these data suggest that 8–12 weeks after crush, damaged cells no longer displayed increased susceptibility to BAX activation relative to their naïve counterparts. In this same timeframe, retinal glial activation and the signaling of the pro-apoptotic JNK pathway also abated. Quiescent RGCs did not show a timely reactivation of their JNK pathway following intravitreal injection with anisomycin. These findings demonstrate that lowering the quantity of BAX in RGCs is neuroprotective after acute injury. Damaged RGCs enter a quiescent state months after injury and are no longer responsive to an apoptotic stimulus. Quiescent RGCs will require rejuvenation to reacquire functionality.","lang":"eng"}],"issue":"2","type":"journal_article","language":[{"iso":"eng"}],"doi":"10.1007/s12035-019-01783-7","quality_controlled":"1","isi":1,"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035206/"}],"oa":1,"external_id":{"pmid":["31673950"],"isi":["000493754200001"]},"month":"02","publication_identifier":{"issn":["0893-7648"],"eissn":["1559-1182"]},"date_created":"2019-11-18T14:18:39Z","date_updated":"2023-08-17T14:05:48Z","volume":57,"author":[{"full_name":"Donahue, RJ","last_name":"Donahue","first_name":"RJ"},{"first_name":"Margaret E","last_name":"Maes","id":"3838F452-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9642-1085","full_name":"Maes, Margaret E"},{"first_name":"JA","last_name":"Grosser","full_name":"Grosser, JA"},{"last_name":"Nickells","first_name":"RW","full_name":"Nickells, RW"}],"publication_status":"published","publisher":"Springer Nature","department":[{"_id":"SaSi"}],"year":"2020","acknowledgement":"This work was supported by National Eye Institute grants R01 EY012223 (RWN), R01 EY030123 (RWN), T32 EY027721 (Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison), and a Vision Science Core grant P30 EY016665 (Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison), an unrestricted funding grant from Research to Prevent Blindness (Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison), the Frederick A. Davis Endowment (RWN), and the Mr. and Mrs. George Taylor Foundation (RWN).","pmid":1}]