---
_id: '1321'
abstract:
- lang: eng
text: Most migrating cells extrude their front by the force of actin polymerization.
Polymerization requires an initial nucleation step, which is mediated by factors
establishing either parallel filaments in the case of filopodia or branched filaments
that form the branched lamellipodial network. Branches are considered essential
for regular cell motility and are initiated by the Arp2/3 complex, which in turn
is activated by nucleation-promoting factors of the WASP and WAVE families. Here
we employed rapid amoeboid crawling leukocytes and found that deletion of the
WAVE complex eliminated actin branching and thus lamellipodia formation. The cells
were left with parallel filaments at the leading edge, which translated, depending
on the differentiation status of the cell, into a unipolar pointed cell shape
or cells with multiple filopodia. Remarkably, unipolar cells migrated with increased
speed and enormous directional persistence, while they were unable to turn towards
chemotactic gradients. Cells with multiple filopodia retained chemotactic activity
but their migration was progressively impaired with increasing geometrical complexity
of the extracellular environment. These findings establish that diversified leading
edge protrusions serve as explorative structures while they slow down actual locomotion.
acknowledged_ssus:
- _id: SSU
acknowledgement: "This work was supported by the German Research Foundation (DFG)
Priority Program SP 1464 to T.E.B.S. and M.S., and European Research Council (ERC
GA 281556) and Human Frontiers Program grants to M.S.\r\nService Units of IST Austria
for excellent technical support."
article_processing_charge: No
article_type: original
author:
- first_name: Alexander F
full_name: Leithner, Alexander F
id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
last_name: Leithner
orcid: 0000-0002-1073-744X
- first_name: Alexander
full_name: Eichner, Alexander
id: 4DFA52AE-F248-11E8-B48F-1D18A9856A87
last_name: Eichner
- first_name: Jan
full_name: Müller, Jan
id: AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D
last_name: Müller
- first_name: Anne
full_name: Reversat, Anne
id: 35B76592-F248-11E8-B48F-1D18A9856A87
last_name: Reversat
orcid: 0000-0003-0666-8928
- first_name: Markus
full_name: Brown, Markus
id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
last_name: Brown
- first_name: Jan
full_name: Schwarz, Jan
id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
last_name: Schwarz
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: David
full_name: De Gorter, David
last_name: De Gorter
- first_name: Florian
full_name: Schur, Florian
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
- first_name: Jonathan
full_name: Bayerl, Jonathan
last_name: Bayerl
- first_name: Ingrid
full_name: De Vries, Ingrid
id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
last_name: De Vries
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Frank
full_name: Lai, Frank
last_name: Lai
- first_name: Markus
full_name: Moser, Markus
last_name: Moser
- first_name: Dontscho
full_name: Kerjaschki, Dontscho
last_name: Kerjaschki
- first_name: Klemens
full_name: Rottner, Klemens
last_name: Rottner
- first_name: Victor
full_name: Small, Victor
last_name: Small
- first_name: Theresia
full_name: Stradal, Theresia
last_name: Stradal
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Leithner AF, Eichner A, Müller J, et al. Diversified actin protrusions promote
environmental exploration but are dispensable for locomotion of leukocytes. Nature
Cell Biology. 2016;18:1253-1259. doi:10.1038/ncb3426
apa: Leithner, A. F., Eichner, A., Müller, J., Reversat, A., Brown, M., Schwarz,
J., … Sixt, M. K. (2016). Diversified actin protrusions promote environmental
exploration but are dispensable for locomotion of leukocytes. Nature Cell Biology.
Nature Publishing Group. https://doi.org/10.1038/ncb3426
chicago: Leithner, Alexander F, Alexander Eichner, Jan Müller, Anne Reversat, Markus
Brown, Jan Schwarz, Jack Merrin, et al. “Diversified Actin Protrusions Promote
Environmental Exploration but Are Dispensable for Locomotion of Leukocytes.” Nature
Cell Biology. Nature Publishing Group, 2016. https://doi.org/10.1038/ncb3426.
ieee: A. F. Leithner et al., “Diversified actin protrusions promote environmental
exploration but are dispensable for locomotion of leukocytes,” Nature Cell
Biology, vol. 18. Nature Publishing Group, pp. 1253–1259, 2016.
ista: Leithner AF, Eichner A, Müller J, Reversat A, Brown M, Schwarz J, Merrin J,
De Gorter D, Schur FK, Bayerl J, de Vries I, Wieser S, Hauschild R, Lai F, Moser
M, Kerjaschki D, Rottner K, Small V, Stradal T, Sixt MK. 2016. Diversified actin
protrusions promote environmental exploration but are dispensable for locomotion
of leukocytes. Nature Cell Biology. 18, 1253–1259.
mla: Leithner, Alexander F., et al. “Diversified Actin Protrusions Promote Environmental
Exploration but Are Dispensable for Locomotion of Leukocytes.” Nature Cell
Biology, vol. 18, Nature Publishing Group, 2016, pp. 1253–59, doi:10.1038/ncb3426.
short: A.F. Leithner, A. Eichner, J. Müller, A. Reversat, M. Brown, J. Schwarz,
J. Merrin, D. De Gorter, F.K. Schur, J. Bayerl, I. de Vries, S. Wieser, R. Hauschild,
F. Lai, M. Moser, D. Kerjaschki, K. Rottner, V. Small, T. Stradal, M.K. Sixt,
Nature Cell Biology 18 (2016) 1253–1259.
date_created: 2018-12-11T11:51:21Z
date_published: 2016-10-24T00:00:00Z
date_updated: 2024-03-28T23:30:16Z
day: '24'
ddc:
- '570'
department:
- _id: MiSi
- _id: NanoFab
- _id: Bio
doi: 10.1038/ncb3426
ec_funded: 1
file:
- access_level: open_access
checksum: e1411cb7c99a2d9089c178a6abef25e7
content_type: application/pdf
creator: dernst
date_created: 2020-05-14T16:33:46Z
date_updated: 2020-07-14T12:44:43Z
file_id: '7844'
file_name: 2018_NatureCell_Leithner.pdf
file_size: 4433280
relation: main_file
file_date_updated: 2020-07-14T12:44:43Z
has_accepted_license: '1'
intvolume: ' 18'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 1253 - 1259
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
(EU)
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '5949'
quality_controlled: '1'
related_material:
record:
- id: '323'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Diversified actin protrusions promote environmental exploration but are dispensable
for locomotion of leukocytes
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 18
year: '2016'
...
---
_id: '1530'
abstract:
- lang: eng
text: In growing cells, protein synthesis and cell growth are typically not synchronous,
and, thus, protein concentrations vary over the cell division cycle. We have developed
a theoretical description of genetic regulatory systems in bacteria that explicitly
considers the cell division cycle to investigate its impact on gene expression.
We calculate the cell-to-cell variations arising from cells being at different
stages in the division cycle for unregulated genes and for basic regulatory mechanisms.
These variations contribute to the extrinsic noise observed in single-cell experiments,
and are most significant for proteins with short lifetimes. Negative autoregulation
buffers against variation of protein concentration over the division cycle, but
the effect is found to be relatively weak. Stronger buffering is achieved by an
increased protein lifetime. Positive autoregulation can strongly amplify such
variation if the parameters are set to values that lead to resonance-like behaviour.
For cooperative positive autoregulation, the concentration variation over the
division cycle diminishes the parameter region of bistability and modulates the
switching times between the two stable states. The same effects are seen for a
two-gene mutual-repression toggle switch. By contrast, an oscillatory circuit,
the repressilator, is only weakly affected by the division cycle.
article_number: '066003'
author:
- first_name: Veronika
full_name: Bierbaum, Veronika
id: 3FD04378-F248-11E8-B48F-1D18A9856A87
last_name: Bierbaum
- first_name: Stefan
full_name: Klumpp, Stefan
last_name: Klumpp
citation:
ama: Bierbaum V, Klumpp S. Impact of the cell division cycle on gene circuits. Physical
Biology. 2015;12(6). doi:10.1088/1478-3975/12/6/066003
apa: Bierbaum, V., & Klumpp, S. (2015). Impact of the cell division cycle on
gene circuits. Physical Biology. IOP Publishing Ltd. https://doi.org/10.1088/1478-3975/12/6/066003
chicago: Bierbaum, Veronika, and Stefan Klumpp. “Impact of the Cell Division Cycle
on Gene Circuits.” Physical Biology. IOP Publishing Ltd., 2015. https://doi.org/10.1088/1478-3975/12/6/066003.
ieee: V. Bierbaum and S. Klumpp, “Impact of the cell division cycle on gene circuits,”
Physical Biology, vol. 12, no. 6. IOP Publishing Ltd., 2015.
ista: Bierbaum V, Klumpp S. 2015. Impact of the cell division cycle on gene circuits.
Physical Biology. 12(6), 066003.
mla: Bierbaum, Veronika, and Stefan Klumpp. “Impact of the Cell Division Cycle on
Gene Circuits.” Physical Biology, vol. 12, no. 6, 066003, IOP Publishing
Ltd., 2015, doi:10.1088/1478-3975/12/6/066003.
short: V. Bierbaum, S. Klumpp, Physical Biology 12 (2015).
date_created: 2018-12-11T11:52:33Z
date_published: 2015-09-25T00:00:00Z
date_updated: 2021-01-12T06:51:25Z
day: '25'
department:
- _id: MiSi
doi: 10.1088/1478-3975/12/6/066003
intvolume: ' 12'
issue: '6'
language:
- iso: eng
month: '09'
oa_version: None
publication: Physical Biology
publication_status: published
publisher: IOP Publishing Ltd.
publist_id: '5641'
quality_controlled: '1'
scopus_import: 1
status: public
title: Impact of the cell division cycle on gene circuits
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2015'
...
---
_id: '1553'
abstract:
- lang: eng
text: Cell movement has essential functions in development, immunity, and cancer.
Various cell migration patterns have been reported, but no general rule has emerged
so far. Here, we show on the basis of experimental data in vitro and in vivo that
cell persistence, which quantifies the straightness of trajectories, is robustly
coupled to cell migration speed. We suggest that this universal coupling constitutes
a generic law of cell migration, which originates in the advection of polarity
cues by an actin cytoskeleton undergoing flows at the cellular scale. Our analysis
relies on a theoretical model that we validate by measuring the persistence of
cells upon modulation of actin flow speeds and upon optogenetic manipulation of
the binding of an actin regulator to actin filaments. Beyond the quantitative
prediction of the coupling, the model yields a generic phase diagram of cellular
trajectories, which recapitulates the full range of observed migration patterns.
author:
- first_name: Paolo
full_name: Maiuri, Paolo
last_name: Maiuri
- first_name: Jean
full_name: Rupprecht, Jean
last_name: Rupprecht
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Olivier
full_name: Bénichou, Olivier
last_name: Bénichou
- first_name: Nicolas
full_name: Carpi, Nicolas
last_name: Carpi
- first_name: Mathieu
full_name: Coppey, Mathieu
last_name: Coppey
- first_name: Simon
full_name: De Beco, Simon
last_name: De Beco
- first_name: Nir
full_name: Gov, Nir
last_name: Gov
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Carolina
full_name: Lage Crespo, Carolina
last_name: Lage Crespo
- first_name: Franziska
full_name: Lautenschlaeger, Franziska
last_name: Lautenschlaeger
- first_name: Maël
full_name: Le Berre, Maël
last_name: Le Berre
- first_name: Ana
full_name: Lennon Duménil, Ana
last_name: Lennon Duménil
- first_name: Matthew
full_name: Raab, Matthew
last_name: Raab
- first_name: Hawa
full_name: Thiam, Hawa
last_name: Thiam
- first_name: Matthieu
full_name: Piel, Matthieu
last_name: Piel
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
citation:
ama: Maiuri P, Rupprecht J, Wieser S, et al. Actin flows mediate a universal coupling
between cell speed and cell persistence. Cell. 2015;161(2):374-386. doi:10.1016/j.cell.2015.01.056
apa: Maiuri, P., Rupprecht, J., Wieser, S., Ruprecht, V., Bénichou, O., Carpi, N.,
… Voituriez, R. (2015). Actin flows mediate a universal coupling between cell
speed and cell persistence. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.01.056
chicago: Maiuri, Paolo, Jean Rupprecht, Stefan Wieser, Verena Ruprecht, Olivier
Bénichou, Nicolas Carpi, Mathieu Coppey, et al. “Actin Flows Mediate a Universal
Coupling between Cell Speed and Cell Persistence.” Cell. Cell Press, 2015.
https://doi.org/10.1016/j.cell.2015.01.056.
ieee: P. Maiuri et al., “Actin flows mediate a universal coupling between
cell speed and cell persistence,” Cell, vol. 161, no. 2. Cell Press, pp.
374–386, 2015.
ista: Maiuri P, Rupprecht J, Wieser S, Ruprecht V, Bénichou O, Carpi N, Coppey M,
De Beco S, Gov N, Heisenberg C-PJ, Lage Crespo C, Lautenschlaeger F, Le Berre
M, Lennon Duménil A, Raab M, Thiam H, Piel M, Sixt MK, Voituriez R. 2015. Actin
flows mediate a universal coupling between cell speed and cell persistence. Cell.
161(2), 374–386.
mla: Maiuri, Paolo, et al. “Actin Flows Mediate a Universal Coupling between Cell
Speed and Cell Persistence.” Cell, vol. 161, no. 2, Cell Press, 2015, pp.
374–86, doi:10.1016/j.cell.2015.01.056.
short: P. Maiuri, J. Rupprecht, S. Wieser, V. Ruprecht, O. Bénichou, N. Carpi, M.
Coppey, S. De Beco, N. Gov, C.-P.J. Heisenberg, C. Lage Crespo, F. Lautenschlaeger,
M. Le Berre, A. Lennon Duménil, M. Raab, H. Thiam, M. Piel, M.K. Sixt, R. Voituriez,
Cell 161 (2015) 374–386.
date_created: 2018-12-11T11:52:41Z
date_published: 2015-04-09T00:00:00Z
date_updated: 2021-01-12T06:51:33Z
day: '09'
department:
- _id: MiSi
- _id: CaHe
doi: 10.1016/j.cell.2015.01.056
ec_funded: 1
intvolume: ' 161'
issue: '2'
language:
- iso: eng
month: '04'
oa_version: None
page: 374 - 386
project:
- _id: 2529486C-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: T 560-B17
name: Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
(EU)
- _id: 25ABD200-B435-11E9-9278-68D0E5697425
grant_number: RGP0058/2011
name: 'Cell migration in complex environments: from in vivo experiments to theoretical
models'
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '5618'
quality_controlled: '1'
scopus_import: 1
status: public
title: Actin flows mediate a universal coupling between cell speed and cell persistence
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 161
year: '2015'
...
---
_id: '1561'
abstract:
- lang: eng
text: Replication-deficient recombinant adenoviruses are potent vectors for the
efficient transient expression of exogenous genes in resting immune cells. However,
most leukocytes are refractory to efficient adenoviral transduction as they lack
expression of the coxsackie/adenovirus receptor (CAR). To circumvent this obstacle,
we generated the R26/CAG-CARΔ1StopF (where R26 is ROSA26 and CAG is CMV early
enhancer/chicken β actin promoter) knock-in mouse line. This strain allows monitoring
of in situ Cre recombinase activity through expression of CARΔ1. Simultaneously,
CARΔ1 expression permits selective and highly efficient adenoviral transduction
of immune cell populations, such as mast cells or T cells, directly ex vivo in
bulk cultures without prior cell purification or activation. Furthermore, we show
that CARΔ1 expression dramatically improves adenoviral infection of in vitro differentiated
conventional and plasmacytoid dendritic cells (DCs), basophils, mast cells, as
well as Hoxb8-immortalized hematopoietic progenitor cells. This novel dual function
mouse strain will hence be a valuable tool to rapidly dissect the function of
specific genes in leukocyte physiology.
author:
- first_name: Klaus
full_name: Heger, Klaus
last_name: Heger
- first_name: Maike
full_name: Kober, Maike
last_name: Kober
- first_name: David
full_name: Rieß, David
last_name: Rieß
- first_name: Christoph
full_name: Drees, Christoph
last_name: Drees
- first_name: Ingrid
full_name: De Vries, Ingrid
id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
last_name: De Vries
- first_name: Arianna
full_name: Bertossi, Arianna
last_name: Bertossi
- first_name: Axel
full_name: Roers, Axel
last_name: Roers
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Marc
full_name: Schmidt Supprian, Marc
last_name: Schmidt Supprian
citation:
ama: Heger K, Kober M, Rieß D, et al. A novel Cre recombinase reporter mouse strain
facilitates selective and efficient infection of primary immune cells with adenoviral
vectors. European Journal of Immunology. 2015;45(6):1614-1620. doi:10.1002/eji.201545457
apa: Heger, K., Kober, M., Rieß, D., Drees, C., de Vries, I., Bertossi, A., … Schmidt
Supprian, M. (2015). A novel Cre recombinase reporter mouse strain facilitates
selective and efficient infection of primary immune cells with adenoviral vectors.
European Journal of Immunology. Wiley. https://doi.org/10.1002/eji.201545457
chicago: Heger, Klaus, Maike Kober, David Rieß, Christoph Drees, Ingrid de Vries,
Arianna Bertossi, Axel Roers, Michael K Sixt, and Marc Schmidt Supprian. “A Novel
Cre Recombinase Reporter Mouse Strain Facilitates Selective and Efficient Infection
of Primary Immune Cells with Adenoviral Vectors.” European Journal of Immunology.
Wiley, 2015. https://doi.org/10.1002/eji.201545457.
ieee: K. Heger et al., “A novel Cre recombinase reporter mouse strain facilitates
selective and efficient infection of primary immune cells with adenoviral vectors,”
European Journal of Immunology, vol. 45, no. 6. Wiley, pp. 1614–1620, 2015.
ista: Heger K, Kober M, Rieß D, Drees C, de Vries I, Bertossi A, Roers A, Sixt MK,
Schmidt Supprian M. 2015. A novel Cre recombinase reporter mouse strain facilitates
selective and efficient infection of primary immune cells with adenoviral vectors.
European Journal of Immunology. 45(6), 1614–1620.
mla: Heger, Klaus, et al. “A Novel Cre Recombinase Reporter Mouse Strain Facilitates
Selective and Efficient Infection of Primary Immune Cells with Adenoviral Vectors.”
European Journal of Immunology, vol. 45, no. 6, Wiley, 2015, pp. 1614–20,
doi:10.1002/eji.201545457.
short: K. Heger, M. Kober, D. Rieß, C. Drees, I. de Vries, A. Bertossi, A. Roers,
M.K. Sixt, M. Schmidt Supprian, European Journal of Immunology 45 (2015) 1614–1620.
date_created: 2018-12-11T11:52:44Z
date_published: 2015-06-01T00:00:00Z
date_updated: 2021-01-12T06:51:36Z
day: '01'
department:
- _id: MiSi
doi: 10.1002/eji.201545457
intvolume: ' 45'
issue: '6'
language:
- iso: eng
month: '06'
oa_version: None
page: 1614 - 1620
publication: European Journal of Immunology
publication_status: published
publisher: Wiley
publist_id: '5610'
quality_controlled: '1'
scopus_import: 1
status: public
title: A novel Cre recombinase reporter mouse strain facilitates selective and efficient
infection of primary immune cells with adenoviral vectors
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 45
year: '2015'
...
---
_id: '1560'
abstract:
- lang: eng
text: Stromal cells in the subcapsular sinus of the lymph node 'decide' which cells
and molecules are allowed access to the deeper parenchyma. The glycoprotein PLVAP
is a crucial component of this selector function.
author:
- first_name: Miroslav
full_name: Hons, Miroslav
id: 4167FE56-F248-11E8-B48F-1D18A9856A87
last_name: Hons
orcid: 0000-0002-6625-3348
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Hons M, Sixt MK. The lymph node filter revealed. Nature Immunology.
2015;16(4):338-340. doi:10.1038/ni.3126
apa: Hons, M., & Sixt, M. K. (2015). The lymph node filter revealed. Nature
Immunology. Nature Publishing Group. https://doi.org/10.1038/ni.3126
chicago: Hons, Miroslav, and Michael K Sixt. “The Lymph Node Filter Revealed.” Nature
Immunology. Nature Publishing Group, 2015. https://doi.org/10.1038/ni.3126.
ieee: M. Hons and M. K. Sixt, “The lymph node filter revealed,” Nature Immunology,
vol. 16, no. 4. Nature Publishing Group, pp. 338–340, 2015.
ista: Hons M, Sixt MK. 2015. The lymph node filter revealed. Nature Immunology.
16(4), 338–340.
mla: Hons, Miroslav, and Michael K. Sixt. “The Lymph Node Filter Revealed.” Nature
Immunology, vol. 16, no. 4, Nature Publishing Group, 2015, pp. 338–40, doi:10.1038/ni.3126.
short: M. Hons, M.K. Sixt, Nature Immunology 16 (2015) 338–340.
date_created: 2018-12-11T11:52:43Z
date_published: 2015-03-19T00:00:00Z
date_updated: 2021-01-12T06:51:36Z
day: '19'
department:
- _id: MiSi
doi: 10.1038/ni.3126
intvolume: ' 16'
issue: '4'
language:
- iso: eng
month: '03'
oa_version: None
page: 338 - 340
publication: Nature Immunology
publication_status: published
publisher: Nature Publishing Group
publist_id: '5611'
quality_controlled: '1'
scopus_import: 1
status: public
title: The lymph node filter revealed
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2015'
...
---
_id: '1575'
abstract:
- lang: eng
text: The immune response relies on the migration of leukocytes and on their ability
to stop in precise anatomical locations to fulfil their task. How leukocyte migration
and function are coordinated is unknown. Here we show that in immature dendritic
cells, which patrol their environment by engulfing extracellular material, cell
migration and antigen capture are antagonistic. This antagonism results from transient
enrichment of myosin IIA at the cell front, which disrupts the back-to-front gradient
of the motor protein, slowing down locomotion but promoting antigen capture. We
further highlight that myosin IIA enrichment at the cell front requires the MHC
class II-associated invariant chain (Ii). Thus, by controlling myosin IIA localization,
Ii imposes on dendritic cells an intermittent antigen capture behaviour that might
facilitate environment patrolling. We propose that the requirement for myosin
II in both cell migration and specific cell functions may provide a general mechanism
for their coordination in time and space.
acknowledgement: M.C. and M.L.H. were supported by fellowships from the Fondation
pour la Recherche Médicale and the Association pour la Recherche contre le Cancer,
respectively. This work was funded by grants from the City of Paris and the European
Research Council to A.-M.L.-D. (Strapacemi 243103), the Association Nationale pour
la Recherche (ANR-09-PIRI-0027-PCVI) and the InnaBiosanté foundation (Micemico)
to A.-M.L.-D., M.P. and R.V., and the DCBIOL Labex from the French Government (ANR-10-IDEX-0001-02-PSL*
and ANR-11-LABX-0043). The super-resolution SIM microscope was funded through an
ERC Advanced Investigator Grant (250367) to Edith Heard (CNRS UMR3215/Inserm U934,
Institut Curie).
article_number: '7526'
author:
- first_name: Mélanie
full_name: Chabaud, Mélanie
last_name: Chabaud
- first_name: Mélina
full_name: Heuzé, Mélina
last_name: Heuzé
- first_name: Marine
full_name: Bretou, Marine
last_name: Bretou
- first_name: Pablo
full_name: Vargas, Pablo
last_name: Vargas
- first_name: Paolo
full_name: Maiuri, Paolo
last_name: Maiuri
- first_name: Paola
full_name: Solanes, Paola
last_name: Solanes
- first_name: Mathieu
full_name: Maurin, Mathieu
last_name: Maurin
- first_name: Emmanuel
full_name: Terriac, Emmanuel
last_name: Terriac
- first_name: Maël
full_name: Le Berre, Maël
last_name: Le Berre
- first_name: Danielle
full_name: Lankar, Danielle
last_name: Lankar
- first_name: Tristan
full_name: Piolot, Tristan
last_name: Piolot
- first_name: Robert
full_name: Adelstein, Robert
last_name: Adelstein
- first_name: Yingfan
full_name: Zhang, Yingfan
last_name: Zhang
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Jordan
full_name: Jacobelli, Jordan
last_name: Jacobelli
- first_name: Olivier
full_name: Bénichou, Olivier
last_name: Bénichou
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
- first_name: Matthieu
full_name: Piel, Matthieu
last_name: Piel
- first_name: Ana
full_name: Lennon Duménil, Ana
last_name: Lennon Duménil
citation:
ama: Chabaud M, Heuzé M, Bretou M, et al. Cell migration and antigen capture are
antagonistic processes coupled by myosin II in dendritic cells. Nature Communications.
2015;6. doi:10.1038/ncomms8526
apa: Chabaud, M., Heuzé, M., Bretou, M., Vargas, P., Maiuri, P., Solanes, P., …
Lennon Duménil, A. (2015). Cell migration and antigen capture are antagonistic
processes coupled by myosin II in dendritic cells. Nature Communications.
Nature Publishing Group. https://doi.org/10.1038/ncomms8526
chicago: Chabaud, Mélanie, Mélina Heuzé, Marine Bretou, Pablo Vargas, Paolo Maiuri,
Paola Solanes, Mathieu Maurin, et al. “Cell Migration and Antigen Capture Are
Antagonistic Processes Coupled by Myosin II in Dendritic Cells.” Nature Communications.
Nature Publishing Group, 2015. https://doi.org/10.1038/ncomms8526.
ieee: M. Chabaud et al., “Cell migration and antigen capture are antagonistic
processes coupled by myosin II in dendritic cells,” Nature Communications,
vol. 6. Nature Publishing Group, 2015.
ista: Chabaud M, Heuzé M, Bretou M, Vargas P, Maiuri P, Solanes P, Maurin M, Terriac
E, Le Berre M, Lankar D, Piolot T, Adelstein R, Zhang Y, Sixt MK, Jacobelli J,
Bénichou O, Voituriez R, Piel M, Lennon Duménil A. 2015. Cell migration and antigen
capture are antagonistic processes coupled by myosin II in dendritic cells. Nature
Communications. 6, 7526.
mla: Chabaud, Mélanie, et al. “Cell Migration and Antigen Capture Are Antagonistic
Processes Coupled by Myosin II in Dendritic Cells.” Nature Communications,
vol. 6, 7526, Nature Publishing Group, 2015, doi:10.1038/ncomms8526.
short: M. Chabaud, M. Heuzé, M. Bretou, P. Vargas, P. Maiuri, P. Solanes, M. Maurin,
E. Terriac, M. Le Berre, D. Lankar, T. Piolot, R. Adelstein, Y. Zhang, M.K. Sixt,
J. Jacobelli, O. Bénichou, R. Voituriez, M. Piel, A. Lennon Duménil, Nature Communications
6 (2015).
date_created: 2018-12-11T11:52:48Z
date_published: 2015-06-25T00:00:00Z
date_updated: 2021-01-12T06:51:42Z
day: '25'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1038/ncomms8526
file:
- access_level: open_access
checksum: bae12e86be2adb28253f890b8bba8315
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:58Z
date_updated: 2020-07-14T12:45:02Z
file_id: '4915'
file_name: IST-2016-476-v1+1_ncomms8526.pdf
file_size: 4530215
relation: main_file
file_date_updated: 2020-07-14T12:45:02Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5596'
pubrep_id: '476'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cell migration and antigen capture are antagonistic processes coupled by myosin
II in dendritic cells
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2015'
...
---
_id: '1676'
author:
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Erez
full_name: Raz, Erez
last_name: Raz
citation:
ama: 'Sixt MK, Raz E. Editorial overview: Cell adhesion and migration. Current
Opinion in Cell Biology. 2015;36(10):4-6. doi:10.1016/j.ceb.2015.09.004'
apa: 'Sixt, M. K., & Raz, E. (2015). Editorial overview: Cell adhesion and migration.
Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2015.09.004'
chicago: 'Sixt, Michael K, and Erez Raz. “Editorial Overview: Cell Adhesion and
Migration.” Current Opinion in Cell Biology. Elsevier, 2015. https://doi.org/10.1016/j.ceb.2015.09.004.'
ieee: 'M. K. Sixt and E. Raz, “Editorial overview: Cell adhesion and migration,”
Current Opinion in Cell Biology, vol. 36, no. 10. Elsevier, pp. 4–6, 2015.'
ista: 'Sixt MK, Raz E. 2015. Editorial overview: Cell adhesion and migration. Current
Opinion in Cell Biology. 36(10), 4–6.'
mla: 'Sixt, Michael K., and Erez Raz. “Editorial Overview: Cell Adhesion and Migration.”
Current Opinion in Cell Biology, vol. 36, no. 10, Elsevier, 2015, pp. 4–6,
doi:10.1016/j.ceb.2015.09.004.'
short: M.K. Sixt, E. Raz, Current Opinion in Cell Biology 36 (2015) 4–6.
date_created: 2018-12-11T11:53:25Z
date_published: 2015-10-01T00:00:00Z
date_updated: 2021-01-12T06:52:27Z
day: '01'
department:
- _id: MiSi
doi: 10.1016/j.ceb.2015.09.004
intvolume: ' 36'
issue: '10'
language:
- iso: eng
month: '10'
oa_version: None
page: 4 - 6
publication: Current Opinion in Cell Biology
publication_status: published
publisher: Elsevier
publist_id: '5473'
scopus_import: 1
status: public
title: 'Editorial overview: Cell adhesion and migration'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2015'
...
---
_id: '1687'
abstract:
- lang: eng
text: Guided cell movement is essential for development and integrity of animals
and crucially involved in cellular immune responses. Leukocytes are professional
migratory cells that can navigate through most types of tissues and sense a wide
range of directional cues. The responses of these cells to attractants have been
mainly explored in tissue culture settings. How leukocytes make directional decisions
in situ, within the challenging environment of a tissue maze, is less understood.
Here we review recent advances in how leukocytes sense chemical cues in complex
tissue settings and make links with paradigms of directed migration in development
and Dictyostelium discoideum amoebae.
author:
- first_name: Milka
full_name: Sarris, Milka
last_name: Sarris
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: 'Sarris M, Sixt MK. Navigating in tissue mazes: Chemoattractant interpretation
in complex environments. Current Opinion in Cell Biology. 2015;36(10):93-102.
doi:10.1016/j.ceb.2015.08.001'
apa: 'Sarris, M., & Sixt, M. K. (2015). Navigating in tissue mazes: Chemoattractant
interpretation in complex environments. Current Opinion in Cell Biology.
Elsevier. https://doi.org/10.1016/j.ceb.2015.08.001'
chicago: 'Sarris, Milka, and Michael K Sixt. “Navigating in Tissue Mazes: Chemoattractant
Interpretation in Complex Environments.” Current Opinion in Cell Biology.
Elsevier, 2015. https://doi.org/10.1016/j.ceb.2015.08.001.'
ieee: 'M. Sarris and M. K. Sixt, “Navigating in tissue mazes: Chemoattractant interpretation
in complex environments,” Current Opinion in Cell Biology, vol. 36, no.
10. Elsevier, pp. 93–102, 2015.'
ista: 'Sarris M, Sixt MK. 2015. Navigating in tissue mazes: Chemoattractant interpretation
in complex environments. Current Opinion in Cell Biology. 36(10), 93–102.'
mla: 'Sarris, Milka, and Michael K. Sixt. “Navigating in Tissue Mazes: Chemoattractant
Interpretation in Complex Environments.” Current Opinion in Cell Biology,
vol. 36, no. 10, Elsevier, 2015, pp. 93–102, doi:10.1016/j.ceb.2015.08.001.'
short: M. Sarris, M.K. Sixt, Current Opinion in Cell Biology 36 (2015) 93–102.
date_created: 2018-12-11T11:53:28Z
date_published: 2015-10-01T00:00:00Z
date_updated: 2021-01-12T06:52:31Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1016/j.ceb.2015.08.001
ec_funded: 1
file:
- access_level: open_access
checksum: c29973924b790aab02fdd91857759cfb
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:21Z
date_updated: 2020-07-14T12:45:12Z
file_id: '4875'
file_name: IST-2016-445-v1+1_1-s2.0-S0955067415001064-main.pdf
file_size: 797964
relation: main_file
file_date_updated: 2020-07-14T12:45:12Z
has_accepted_license: '1'
intvolume: ' 36'
issue: '10'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 93 - 102
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
(EU)
publication: Current Opinion in Cell Biology
publication_status: published
publisher: Elsevier
publist_id: '5458'
pubrep_id: '445'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Navigating in tissue mazes: Chemoattractant interpretation in complex environments'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2015'
...
---
_id: '1686'
author:
- first_name: Eva
full_name: Kiermaier, Eva
id: 3EB04B78-F248-11E8-B48F-1D18A9856A87
last_name: Kiermaier
orcid: 0000-0001-6165-5738
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: 'Kiermaier E, Sixt MK. Fragmented communication between immune cells: Neutrophils
blaze a trail with migratory cues for T cells to follow to sites of infection.
Science. 2015;349(6252):1055-1056. doi:10.1126/science.aad0867'
apa: 'Kiermaier, E., & Sixt, M. K. (2015). Fragmented communication between
immune cells: Neutrophils blaze a trail with migratory cues for T cells to follow
to sites of infection. Science. American Association for the Advancement
of Science. https://doi.org/10.1126/science.aad0867'
chicago: 'Kiermaier, Eva, and Michael K Sixt. “Fragmented Communication between
Immune Cells: Neutrophils Blaze a Trail with Migratory Cues for T Cells to Follow
to Sites of Infection.” Science. American Association for the Advancement
of Science, 2015. https://doi.org/10.1126/science.aad0867.'
ieee: 'E. Kiermaier and M. K. Sixt, “Fragmented communication between immune cells:
Neutrophils blaze a trail with migratory cues for T cells to follow to sites of
infection,” Science, vol. 349, no. 6252. American Association for the Advancement
of Science, pp. 1055–1056, 2015.'
ista: 'Kiermaier E, Sixt MK. 2015. Fragmented communication between immune cells:
Neutrophils blaze a trail with migratory cues for T cells to follow to sites of
infection. Science. 349(6252), 1055–1056.'
mla: 'Kiermaier, Eva, and Michael K. Sixt. “Fragmented Communication between Immune
Cells: Neutrophils Blaze a Trail with Migratory Cues for T Cells to Follow to
Sites of Infection.” Science, vol. 349, no. 6252, American Association
for the Advancement of Science, 2015, pp. 1055–56, doi:10.1126/science.aad0867.'
short: E. Kiermaier, M.K. Sixt, Science 349 (2015) 1055–1056.
date_created: 2018-12-11T11:53:28Z
date_published: 2015-09-04T00:00:00Z
date_updated: 2021-01-12T06:52:31Z
day: '04'
department:
- _id: MiSi
doi: 10.1126/science.aad0867
intvolume: ' 349'
issue: '6252'
language:
- iso: eng
month: '09'
oa_version: None
page: 1055 - 1056
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '5459'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Fragmented communication between immune cells: Neutrophils blaze a trail with
migratory cues for T cells to follow to sites of infection'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 349
year: '2015'
...
---
_id: '477'
abstract:
- lang: eng
text: Dendritic cells are potent antigen-presenting cells endowed with the unique
ability to initiate adaptive immune responses upon inflammation. Inflammatory
processes are often associated with an increased production of serotonin, which
operates by activating specific receptors. However, the functional role of serotonin
receptors in regulation of dendritic cell functions is poorly understood. Here,
we demonstrate that expression of serotonin receptor 5-HT7 (5-HT7TR) as well as
its downstream effector Cdc42 is upregulated in dendritic cells upon maturation.
Although dendritic cell maturation was independent of 5-HT7TR, receptor stimulation
affected dendritic cell morphology through Cdc42-mediated signaling. In addition,
basal activity of 5-HT7TR was required for the proper expression of the chemokine
receptor CCR7, which is a key factor that controls dendritic cell migration. Consistent
with this, we observed that 5-HT7TR enhances chemotactic motility of dendritic
cells in vitro by modulating their directionality and migration velocity. Accordingly,
migration of dendritic cells in murine colon explants was abolished after pharmacological
receptor inhibition. Our results indicate that there is a crucial role for 5-HT7TR-Cdc42-mediated
signaling in the regulation of dendritic cell morphology and motility, suggesting
that 5-HT7TR could be a new target for treatment of a variety of inflammatory
and immune disorders.
author:
- first_name: Katrin
full_name: Holst, Katrin
last_name: Holst
- first_name: Daria
full_name: Guseva, Daria
last_name: Guseva
- first_name: Susann
full_name: Schindler, Susann
last_name: Schindler
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Armin
full_name: Braun, Armin
last_name: Braun
- first_name: Himpriya
full_name: Chopra, Himpriya
last_name: Chopra
- first_name: Oliver
full_name: Pabst, Oliver
last_name: Pabst
- first_name: Evgeni
full_name: Ponimaskin, Evgeni
last_name: Ponimaskin
citation:
ama: Holst K, Guseva D, Schindler S, et al. The serotonin receptor 5-HT7R regulates
the morphology and migratory properties of dendritic cells. Journal of Cell
Science. 2015;128(15):2866-2880. doi:10.1242/jcs.167999
apa: Holst, K., Guseva, D., Schindler, S., Sixt, M. K., Braun, A., Chopra, H., …
Ponimaskin, E. (2015). The serotonin receptor 5-HT7R regulates the morphology
and migratory properties of dendritic cells. Journal of Cell Science. Company
of Biologists. https://doi.org/10.1242/jcs.167999
chicago: Holst, Katrin, Daria Guseva, Susann Schindler, Michael K Sixt, Armin Braun,
Himpriya Chopra, Oliver Pabst, and Evgeni Ponimaskin. “The Serotonin Receptor
5-HT7R Regulates the Morphology and Migratory Properties of Dendritic Cells.”
Journal of Cell Science. Company of Biologists, 2015. https://doi.org/10.1242/jcs.167999.
ieee: K. Holst et al., “The serotonin receptor 5-HT7R regulates the morphology
and migratory properties of dendritic cells,” Journal of Cell Science,
vol. 128, no. 15. Company of Biologists, pp. 2866–2880, 2015.
ista: Holst K, Guseva D, Schindler S, Sixt MK, Braun A, Chopra H, Pabst O, Ponimaskin
E. 2015. The serotonin receptor 5-HT7R regulates the morphology and migratory
properties of dendritic cells. Journal of Cell Science. 128(15), 2866–2880.
mla: Holst, Katrin, et al. “The Serotonin Receptor 5-HT7R Regulates the Morphology
and Migratory Properties of Dendritic Cells.” Journal of Cell Science,
vol. 128, no. 15, Company of Biologists, 2015, pp. 2866–80, doi:10.1242/jcs.167999.
short: K. Holst, D. Guseva, S. Schindler, M.K. Sixt, A. Braun, H. Chopra, O. Pabst,
E. Ponimaskin, Journal of Cell Science 128 (2015) 2866–2880.
date_created: 2018-12-11T11:46:41Z
date_published: 2015-06-15T00:00:00Z
date_updated: 2021-01-12T08:00:54Z
day: '15'
department:
- _id: MiSi
doi: 10.1242/jcs.167999
intvolume: ' 128'
issue: '15'
language:
- iso: eng
month: '06'
oa_version: None
page: 2866 - 2880
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '7343'
quality_controlled: '1'
scopus_import: 1
status: public
title: The serotonin receptor 5-HT7R regulates the morphology and migratory properties
of dendritic cells
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 128
year: '2015'
...