[{"acknowledgement":"This work was funded by grants from the European Research Council (ERC StG 281556 and CoG 724373) and the Austrian Science Foundation (FWF) to M.S. and by Swiss National Foundation (SNF) project grants 31003A_135649, 31003A_153457 and CR23I3_156234 to J.V.S. F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687, and J.R. was funded by an EMBO long-term fellowship (ALTF 1396-2014).","quality_controlled":"1","publisher":"Nature Publishing Group","oa":1,"day":"18","publication":"Nature Immunology","isi":1,"year":"2018","doi":"10.1038/s41590-018-0109-z","date_published":"2018-05-18T00:00:00Z","date_created":"2018-12-11T11:44:10Z","page":"606 - 616","project":[{"call_identifier":"H2020","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","grant_number":"724373"},{"name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","grant_number":"747687","call_identifier":"H2020","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"},{"_id":"25A48D24-B435-11E9-9278-68D0E5697425","name":"Molecular and system level view of immune cell migration","grant_number":"ALTF 1396-2014"},{"grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7","_id":"25A603A2-B435-11E9-9278-68D0E5697425"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"ista":"Hons M, Kopf A, Hauschild R, Leithner AF, Gärtner FR, Abe J, Renkawitz J, Stein J, Sixt MK. 2018. Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. Nature Immunology. 19(6), 606–616.","chicago":"Hons, Miroslav, Aglaja Kopf, Robert Hauschild, Alexander F Leithner, Florian R Gärtner, Jun Abe, Jörg Renkawitz, Jens Stein, and Michael K Sixt. “Chemokines and Integrins Independently Tune Actin Flow and Substrate Friction during Intranodal Migration of T Cells.” Nature Immunology. Nature Publishing Group, 2018. https://doi.org/10.1038/s41590-018-0109-z.","short":"M. Hons, A. Kopf, R. Hauschild, A.F. Leithner, F.R. Gärtner, J. Abe, J. Renkawitz, J. Stein, M.K. Sixt, Nature Immunology 19 (2018) 606–616.","ieee":"M. Hons et al., “Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells,” Nature Immunology, vol. 19, no. 6. Nature Publishing Group, pp. 606–616, 2018.","ama":"Hons M, Kopf A, Hauschild R, et al. Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. Nature Immunology. 2018;19(6):606-616. doi:10.1038/s41590-018-0109-z","apa":"Hons, M., Kopf, A., Hauschild, R., Leithner, A. F., Gärtner, F. R., Abe, J., … Sixt, M. K. (2018). Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. Nature Immunology. Nature Publishing Group. https://doi.org/10.1038/s41590-018-0109-z","mla":"Hons, Miroslav, et al. “Chemokines and Integrins Independently Tune Actin Flow and Substrate Friction during Intranodal Migration of T Cells.” Nature Immunology, vol. 19, no. 6, Nature Publishing Group, 2018, pp. 606–16, doi:10.1038/s41590-018-0109-z."},"title":"Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells","author":[{"last_name":"Hons","full_name":"Hons, Miroslav","orcid":"0000-0002-6625-3348","first_name":"Miroslav","id":"4167FE56-F248-11E8-B48F-1D18A9856A87"},{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","first_name":"Aglaja","orcid":"0000-0002-2187-6656","full_name":"Kopf, Aglaja","last_name":"Kopf"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"full_name":"Leithner, Alexander F","orcid":"0000-0002-1073-744X","last_name":"Leithner","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander F"},{"last_name":"Gärtner","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R"},{"first_name":"Jun","last_name":"Abe","full_name":"Abe, Jun"},{"first_name":"Jörg","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","last_name":"Renkawitz","full_name":"Renkawitz, Jörg","orcid":"0000-0003-2856-3369"},{"first_name":"Jens","full_name":"Stein, Jens","last_name":"Stein"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"}],"publist_id":"8040","external_id":{"isi":["000433041500026"],"pmid":["29777221"]},"article_processing_charge":"No","pmid":1,"oa_version":"Published Version","abstract":[{"lang":"eng","text":"Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together with analysis of cytoskeletal dynamics in vitro to deconstruct the basic mechanisms of T cell migration within lymphatic organs. We show that the contributions of the integrin LFA-1 and the chemokine receptor CCR7 are complementary rather than positioned in a linear pathway, as they are during leukocyte extravasation from the blood vasculature. Our data demonstrate that CCR7 controls cortical actin flows, whereas integrins mediate substrate friction that is sufficient to drive locomotion in the absence of considerable surface adhesions and plasma membrane flux."}],"acknowledged_ssus":[{"_id":"SSU"}],"month":"05","intvolume":" 19","scopus_import":"1","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/29777221","open_access":"1"}],"language":[{"iso":"eng"}],"publication_status":"published","volume":19,"issue":"6","related_material":{"record":[{"status":"public","id":"6891","relation":"dissertation_contains"}]},"ec_funded":1,"_id":"15","status":"public","type":"journal_article","date_updated":"2024-03-27T23:30:39Z","department":[{"_id":"MiSi"},{"_id":"Bio"}]},{"quality_controlled":"1","publisher":"eLife Sciences Publications","oa":1,"day":"06","publication":"eLife","has_accepted_license":"1","year":"2017","doi":"10.7554/eLife.30867","date_published":"2017-11-06T00:00:00Z","date_created":"2018-12-11T11:47:14Z","article_number":"e30867","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"chicago":"Spira, Felix, Sara Cuylen Haering, Shalin Mehta, Matthias Samwer, Anne Reversat, Amitabh Verma, Rudolf Oldenbourg, Michael K Sixt, and Daniel Gerlich. “Cytokinesis in Vertebrate Cells Initiates by Contraction of an Equatorial Actomyosin Network Composed of Randomly Oriented Filaments.” ELife. eLife Sciences Publications, 2017. https://doi.org/10.7554/eLife.30867.","ista":"Spira F, Cuylen Haering S, Mehta S, Samwer M, Reversat A, Verma A, Oldenbourg R, Sixt MK, Gerlich D. 2017. Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments. eLife. 6, e30867.","mla":"Spira, Felix, et al. “Cytokinesis in Vertebrate Cells Initiates by Contraction of an Equatorial Actomyosin Network Composed of Randomly Oriented Filaments.” ELife, vol. 6, e30867, eLife Sciences Publications, 2017, doi:10.7554/eLife.30867.","apa":"Spira, F., Cuylen Haering, S., Mehta, S., Samwer, M., Reversat, A., Verma, A., … Gerlich, D. (2017). Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.30867","ama":"Spira F, Cuylen Haering S, Mehta S, et al. Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments. eLife. 2017;6. doi:10.7554/eLife.30867","ieee":"F. Spira et al., “Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments,” eLife, vol. 6. eLife Sciences Publications, 2017.","short":"F. Spira, S. Cuylen Haering, S. Mehta, M. Samwer, A. Reversat, A. Verma, R. Oldenbourg, M.K. Sixt, D. Gerlich, ELife 6 (2017)."},"title":"Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments","publist_id":"7245","author":[{"last_name":"Spira","full_name":"Spira, Felix","first_name":"Felix"},{"first_name":"Sara","full_name":"Cuylen Haering, Sara","last_name":"Cuylen Haering"},{"full_name":"Mehta, Shalin","last_name":"Mehta","first_name":"Shalin"},{"first_name":"Matthias","last_name":"Samwer","full_name":"Samwer, Matthias"},{"last_name":"Reversat","orcid":"0000-0003-0666-8928","full_name":"Reversat, Anne","first_name":"Anne","id":"35B76592-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Amitabh","full_name":"Verma, Amitabh","last_name":"Verma"},{"first_name":"Rudolf","full_name":"Oldenbourg, Rudolf","last_name":"Oldenbourg"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt"},{"first_name":"Daniel","last_name":"Gerlich","full_name":"Gerlich, Daniel"}],"oa_version":"Published Version","abstract":[{"lang":"eng","text":"The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order in human cells using fluorescence polarization microscopy and found that cleavage furrow ingression initiates by contraction of an equatorial actin network with randomly oriented filaments. The network subsequently gradually reoriented actin filaments along the cell equator. This strictly depended on myosin II activity, suggesting local network reorganization by mechanical forces. Cortical laser microsurgery revealed that during cytokinesis progression, mechanical tension increased substantially along the direction of the cell equator, while the network contracted laterally along the pole-to-pole axis without a detectable increase in tension. Our data suggest that an asymmetric increase in cortical tension promotes filament reorientation along the cytokinetic cleavage furrow, which might have implications for diverse other biological processes involving actomyosin rings."}],"month":"11","intvolume":" 6","scopus_import":1,"file":[{"file_name":"IST-2017-919-v1+1_elife-30867-figures-v1.pdf","date_created":"2018-12-12T10:10:40Z","file_size":9666973,"date_updated":"2020-07-14T12:47:10Z","creator":"system","checksum":"ba09c1451153d39e4f4b7cee013e314c","file_id":"4829","content_type":"application/pdf","relation":"main_file","access_level":"open_access"},{"checksum":"01eb51f1d6ad679947415a51c988e137","file_id":"4830","content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_name":"IST-2017-919-v1+2_elife-30867-v1.pdf","date_created":"2018-12-12T10:10:41Z","file_size":5951246,"date_updated":"2020-07-14T12:47:10Z","creator":"system"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["2050084X"]},"publication_status":"published","volume":6,"_id":"569","status":"public","pubrep_id":"919","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"date_updated":"2023-02-23T12:30:29Z","file_date_updated":"2020-07-14T12:47:10Z","department":[{"_id":"MiSi"}]},{"project":[{"call_identifier":"H2020","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","grant_number":"747687"}],"publist_id":"7243","author":[{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","orcid":"0000-0001-6120-3723","full_name":"Gärtner, Florian R","last_name":"Gärtner"},{"full_name":"Ahmad, Zerkah","last_name":"Ahmad","first_name":"Zerkah"},{"first_name":"Gerhild","full_name":"Rosenberger, Gerhild","last_name":"Rosenberger"},{"full_name":"Fan, Shuxia","last_name":"Fan","first_name":"Shuxia"},{"last_name":"Nicolai","full_name":"Nicolai, Leo","first_name":"Leo"},{"first_name":"Benjamin","last_name":"Busch","full_name":"Busch, Benjamin"},{"first_name":"Gökce","last_name":"Yavuz","full_name":"Yavuz, Gökce"},{"first_name":"Manja","full_name":"Luckner, Manja","last_name":"Luckner"},{"first_name":"Hellen","last_name":"Ishikawa Ankerhold","full_name":"Ishikawa Ankerhold, Hellen"},{"first_name":"Roman","full_name":"Hennel, Roman","last_name":"Hennel"},{"first_name":"Alexandre","last_name":"Benechet","full_name":"Benechet, Alexandre"},{"last_name":"Lorenz","full_name":"Lorenz, Michael","first_name":"Michael"},{"last_name":"Chandraratne","full_name":"Chandraratne, Sue","first_name":"Sue"},{"first_name":"Irene","last_name":"Schubert","full_name":"Schubert, Irene"},{"full_name":"Helmer, Sebastian","last_name":"Helmer","first_name":"Sebastian"},{"first_name":"Bianca","full_name":"Striednig, Bianca","last_name":"Striednig"},{"first_name":"Konstantin","last_name":"Stark","full_name":"Stark, Konstantin"},{"last_name":"Janko","full_name":"Janko, Marek","first_name":"Marek"},{"full_name":"Böttcher, Ralph","last_name":"Böttcher","first_name":"Ralph"},{"full_name":"Verschoor, Admar","last_name":"Verschoor","first_name":"Admar"},{"first_name":"Catherine","full_name":"Leon, Catherine","last_name":"Leon"},{"last_name":"Gachet","full_name":"Gachet, Christian","first_name":"Christian"},{"last_name":"Gudermann","full_name":"Gudermann, Thomas","first_name":"Thomas"},{"full_name":"Mederos Y Schnitzler, Michael","last_name":"Mederos Y Schnitzler","first_name":"Michael"},{"first_name":"Zachary","last_name":"Pincus","full_name":"Pincus, Zachary"},{"full_name":"Iannacone, Matteo","last_name":"Iannacone","first_name":"Matteo"},{"full_name":"Haas, Rainer","last_name":"Haas","first_name":"Rainer"},{"full_name":"Wanner, Gerhard","last_name":"Wanner","first_name":"Gerhard"},{"first_name":"Kirsten","last_name":"Lauber","full_name":"Lauber, Kirsten"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"},{"first_name":"Steffen","full_name":"Massberg, Steffen","last_name":"Massberg"}],"title":"Migrating platelets are mechano scavengers that collect and bundle bacteria","citation":{"ama":"Gärtner FR, Ahmad Z, Rosenberger G, et al. Migrating platelets are mechano scavengers that collect and bundle bacteria. Cell Press. 2017;171(6):1368-1382. doi:10.1016/j.cell.2017.11.001","apa":"Gärtner, F. R., Ahmad, Z., Rosenberger, G., Fan, S., Nicolai, L., Busch, B., … Massberg, S. (2017). Migrating platelets are mechano scavengers that collect and bundle bacteria. Cell Press. Cell Press. https://doi.org/10.1016/j.cell.2017.11.001","ieee":"F. R. Gärtner et al., “Migrating platelets are mechano scavengers that collect and bundle bacteria,” Cell Press, vol. 171, no. 6. Cell Press, pp. 1368–1382, 2017.","short":"F.R. Gärtner, Z. Ahmad, G. Rosenberger, S. Fan, L. Nicolai, B. Busch, G. Yavuz, M. Luckner, H. Ishikawa Ankerhold, R. Hennel, A. Benechet, M. Lorenz, S. Chandraratne, I. Schubert, S. Helmer, B. Striednig, K. Stark, M. Janko, R. Böttcher, A. Verschoor, C. Leon, C. Gachet, T. Gudermann, M. Mederos Y Schnitzler, Z. Pincus, M. Iannacone, R. Haas, G. Wanner, K. Lauber, M.K. Sixt, S. Massberg, Cell Press 171 (2017) 1368–1382.","mla":"Gärtner, Florian R., et al. “Migrating Platelets Are Mechano Scavengers That Collect and Bundle Bacteria.” Cell Press, vol. 171, no. 6, Cell Press, 2017, pp. 1368–82, doi:10.1016/j.cell.2017.11.001.","ista":"Gärtner FR, Ahmad Z, Rosenberger G, Fan S, Nicolai L, Busch B, Yavuz G, Luckner M, Ishikawa Ankerhold H, Hennel R, Benechet A, Lorenz M, Chandraratne S, Schubert I, Helmer S, Striednig B, Stark K, Janko M, Böttcher R, Verschoor A, Leon C, Gachet C, Gudermann T, Mederos Y Schnitzler M, Pincus Z, Iannacone M, Haas R, Wanner G, Lauber K, Sixt MK, Massberg S. 2017. Migrating platelets are mechano scavengers that collect and bundle bacteria. Cell Press. 171(6), 1368–1382.","chicago":"Gärtner, Florian R, Zerkah Ahmad, Gerhild Rosenberger, Shuxia Fan, Leo Nicolai, Benjamin Busch, Gökce Yavuz, et al. “Migrating Platelets Are Mechano Scavengers That Collect and Bundle Bacteria.” Cell Press. Cell Press, 2017. https://doi.org/10.1016/j.cell.2017.11.001."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Cell Press","quality_controlled":"1","page":"1368 - 1382","date_created":"2018-12-11T11:47:15Z","doi":"10.1016/j.cell.2017.11.001","date_published":"2017-11-30T00:00:00Z","year":"2017","publication":"Cell Press","day":"30","type":"journal_article","status":"public","_id":"571","department":[{"_id":"MiSi"}],"date_updated":"2021-01-12T08:03:15Z","scopus_import":1,"intvolume":" 171","month":"11","abstract":[{"text":"Blood platelets are critical for hemostasis and thrombosis and play diverse roles during immune responses. Despite these versatile tasks in mammalian biology, their skills on a cellular level are deemed limited, mainly consisting in rolling, adhesion, and aggregate formation. Here, we identify an unappreciated asset of platelets and show that adherent platelets use adhesion receptors to mechanically probe the adhesive substrate in their local microenvironment. When actomyosin-dependent traction forces overcome substrate resistance, platelets migrate and pile up the adhesive substrate together with any bound particulate material. They use this ability to act as cellular scavengers, scanning the vascular surface for potential invaders and collecting deposited bacteria. Microbe collection by migrating platelets boosts the activity of professional phagocytes, exacerbating inflammatory tissue injury in sepsis. This assigns platelets a central role in innate immune responses and identifies them as potential targets to dampen inflammatory tissue damage in clinical scenarios of severe systemic infection. In addition to their role in thrombosis and hemostasis, platelets can also migrate to sites of infection to help trap bacteria and clear the vascular surface.","lang":"eng"}],"oa_version":"None","ec_funded":1,"volume":171,"issue":"6","publication_status":"published","publication_identifier":{"issn":["00928674"]},"language":[{"iso":"eng"}]},{"department":[{"_id":"MiSi"}],"file_date_updated":"2020-07-14T12:47:34Z","date_updated":"2021-01-12T08:08:06Z","ddc":["570"],"type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","pubrep_id":"902","_id":"659","volume":8,"publication_identifier":{"issn":["20411723"]},"publication_status":"published","file":[{"file_name":"IST-2017-902-v1+1_Kage_et_al-2017-Nature_Communications.pdf","date_created":"2018-12-12T10:14:21Z","creator":"system","file_size":9523746,"date_updated":"2020-07-14T12:47:34Z","file_id":"5072","checksum":"dae30190291c3630e8102d8714a8d23e","relation":"main_file","access_level":"open_access","content_type":"application/pdf"}],"language":[{"iso":"eng"}],"scopus_import":1,"month":"03","intvolume":" 8","abstract":[{"lang":"eng","text":"Migration frequently involves Rac-mediated protrusion of lamellipodia, formed by Arp2/3 complex-dependent branching thought to be crucial for force generation and stability of these networks. The formins FMNL2 and FMNL3 are Cdc42 effectors targeting to the lamellipodium tip and shown here to nucleate and elongate actin filaments with complementary activities in vitro. In migrating B16-F1 melanoma cells, both formins contribute to the velocity of lamellipodium protrusion. Loss of FMNL2/3 function in melanoma cells and fibroblasts reduces lamellipodial width, actin filament density and -bundling, without changing patterns of Arp2/3 complex incorporation. Strikingly, in melanoma cells, FMNL2/3 gene inactivation almost completely abolishes protrusion forces exerted by lamellipodia and modifies their ultrastructural organization. Consistently, CRISPR/Cas-mediated depletion of FMNL2/3 in fibroblasts reduces both migration and capability of cells to move against viscous media. Together, we conclude that force generation in lamellipodia strongly depends on FMNL formin activity, operating in addition to Arp2/3 complex-dependent filament branching."}],"oa_version":"Published Version","author":[{"first_name":"Frieda","full_name":"Kage, Frieda","last_name":"Kage"},{"full_name":"Winterhoff, Moritz","last_name":"Winterhoff","first_name":"Moritz"},{"first_name":"Vanessa","full_name":"Dimchev, Vanessa","last_name":"Dimchev"},{"full_name":"Müller, Jan","last_name":"Müller","first_name":"Jan","id":"AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D"},{"full_name":"Thalheim, Tobias","last_name":"Thalheim","first_name":"Tobias"},{"first_name":"Anika","last_name":"Freise","full_name":"Freise, Anika"},{"last_name":"Brühmann","full_name":"Brühmann, Stefan","first_name":"Stefan"},{"last_name":"Kollasser","full_name":"Kollasser, Jana","first_name":"Jana"},{"full_name":"Block, Jennifer","last_name":"Block","first_name":"Jennifer"},{"full_name":"Dimchev, Georgi A","last_name":"Dimchev","first_name":"Georgi A"},{"first_name":"Matthias","last_name":"Geyer","full_name":"Geyer, Matthias"},{"full_name":"Schnittler, Hams","last_name":"Schnittler","first_name":"Hams"},{"first_name":"Cord","full_name":"Brakebusch, Cord","last_name":"Brakebusch"},{"full_name":"Stradal, Theresia","last_name":"Stradal","first_name":"Theresia"},{"first_name":"Marie","full_name":"Carlier, Marie","last_name":"Carlier"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"},{"last_name":"Käs","full_name":"Käs, Josef","first_name":"Josef"},{"full_name":"Faix, Jan","last_name":"Faix","first_name":"Jan"},{"first_name":"Klemens","last_name":"Rottner","full_name":"Rottner, Klemens"}],"publist_id":"7075","article_processing_charge":"No","title":"FMNL formins boost lamellipodial force generation","citation":{"chicago":"Kage, Frieda, Moritz Winterhoff, Vanessa Dimchev, Jan Müller, Tobias Thalheim, Anika Freise, Stefan Brühmann, et al. “FMNL Formins Boost Lamellipodial Force Generation.” Nature Communications. Nature Publishing Group, 2017. https://doi.org/10.1038/ncomms14832.","ista":"Kage F, Winterhoff M, Dimchev V, Müller J, Thalheim T, Freise A, Brühmann S, Kollasser J, Block J, Dimchev GA, Geyer M, Schnittler H, Brakebusch C, Stradal T, Carlier M, Sixt MK, Käs J, Faix J, Rottner K. 2017. FMNL formins boost lamellipodial force generation. Nature Communications. 8, 14832.","mla":"Kage, Frieda, et al. “FMNL Formins Boost Lamellipodial Force Generation.” Nature Communications, vol. 8, 14832, Nature Publishing Group, 2017, doi:10.1038/ncomms14832.","short":"F. Kage, M. Winterhoff, V. Dimchev, J. Müller, T. Thalheim, A. Freise, S. Brühmann, J. Kollasser, J. Block, G.A. Dimchev, M. Geyer, H. Schnittler, C. Brakebusch, T. Stradal, M. Carlier, M.K. Sixt, J. Käs, J. Faix, K. Rottner, Nature Communications 8 (2017).","ieee":"F. Kage et al., “FMNL formins boost lamellipodial force generation,” Nature Communications, vol. 8. Nature Publishing Group, 2017.","ama":"Kage F, Winterhoff M, Dimchev V, et al. FMNL formins boost lamellipodial force generation. Nature Communications. 2017;8. doi:10.1038/ncomms14832","apa":"Kage, F., Winterhoff, M., Dimchev, V., Müller, J., Thalheim, T., Freise, A., … Rottner, K. (2017). FMNL formins boost lamellipodial force generation. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms14832"},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","article_number":"14832","date_published":"2017-03-22T00:00:00Z","doi":"10.1038/ncomms14832","date_created":"2018-12-11T11:47:46Z","has_accepted_license":"1","year":"2017","day":"22","publication":"Nature Communications","publisher":"Nature Publishing Group","quality_controlled":"1","oa":1},{"publisher":"American Society for Biochemistry and Molecular Biology","quality_controlled":"1","oa":1,"day":"28","publication":"Journal of Biological Chemistry","has_accepted_license":"1","year":"2017","doi":"10.1074/jbc.M116.766923","date_published":"2017-04-28T00:00:00Z","date_created":"2018-12-11T11:47:49Z","page":"7258 - 7273","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ista":"Horsthemke M, Bachg A, Groll K, Moyzio S, Müther B, Hemkemeyer S, Wedlich Söldner R, Sixt MK, Tacke S, Bähler M, Hanley P. 2017. Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion. Journal of Biological Chemistry. 292(17), 7258–7273.","chicago":"Horsthemke, Markus, Anne Bachg, Katharina Groll, Sven Moyzio, Barbara Müther, Sandra Hemkemeyer, Roland Wedlich Söldner, et al. “Multiple Roles of Filopodial Dynamics in Particle Capture and Phagocytosis and Phenotypes of Cdc42 and Myo10 Deletion.” Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 2017. https://doi.org/10.1074/jbc.M116.766923.","ama":"Horsthemke M, Bachg A, Groll K, et al. Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion. Journal of Biological Chemistry. 2017;292(17):7258-7273. doi:10.1074/jbc.M116.766923","apa":"Horsthemke, M., Bachg, A., Groll, K., Moyzio, S., Müther, B., Hemkemeyer, S., … Hanley, P. (2017). Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion. Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology. https://doi.org/10.1074/jbc.M116.766923","short":"M. Horsthemke, A. Bachg, K. Groll, S. Moyzio, B. Müther, S. Hemkemeyer, R. Wedlich Söldner, M.K. Sixt, S. Tacke, M. Bähler, P. Hanley, Journal of Biological Chemistry 292 (2017) 7258–7273.","ieee":"M. Horsthemke et al., “Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion,” Journal of Biological Chemistry, vol. 292, no. 17. American Society for Biochemistry and Molecular Biology, pp. 7258–7273, 2017.","mla":"Horsthemke, Markus, et al. “Multiple Roles of Filopodial Dynamics in Particle Capture and Phagocytosis and Phenotypes of Cdc42 and Myo10 Deletion.” Journal of Biological Chemistry, vol. 292, no. 17, American Society for Biochemistry and Molecular Biology, 2017, pp. 7258–73, doi:10.1074/jbc.M116.766923."},"title":"Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion","author":[{"full_name":"Horsthemke, Markus","last_name":"Horsthemke","first_name":"Markus"},{"last_name":"Bachg","full_name":"Bachg, Anne","first_name":"Anne"},{"first_name":"Katharina","full_name":"Groll, Katharina","last_name":"Groll"},{"full_name":"Moyzio, Sven","last_name":"Moyzio","first_name":"Sven"},{"first_name":"Barbara","last_name":"Müther","full_name":"Müther, Barbara"},{"full_name":"Hemkemeyer, Sandra","last_name":"Hemkemeyer","first_name":"Sandra"},{"full_name":"Wedlich Söldner, Roland","last_name":"Wedlich Söldner","first_name":"Roland"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"},{"first_name":"Sebastian","full_name":"Tacke, Sebastian","last_name":"Tacke"},{"first_name":"Martin","last_name":"Bähler","full_name":"Bähler, Martin"},{"full_name":"Hanley, Peter","last_name":"Hanley","first_name":"Peter"}],"publist_id":"7059","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Macrophage filopodia, finger-like membrane protrusions, were first implicated in phagocytosis more than 100 years ago, but little is still known about the involvement of these actin-dependent structures in particle clearance. Using spinning disk confocal microscopy to image filopodial dynamics in mouse resident Lifeact-EGFP macrophages, we show that filopodia, or filopodia-like structures, support pathogen clearance by multiple means. Filopodia supported the phagocytic uptake of bacterial (Escherichia coli) particles by (i) capturing along the filopodial shaft and surfing toward the cell body, the most common mode of capture; (ii) capturing via the tip followed by retraction; (iii) combinations of surfing and retraction; or (iv) sweeping actions. In addition, filopodia supported the uptake of zymosan (Saccharomyces cerevisiae) particles by (i) providing fixation, (ii) capturing at the tip and filopodia-guided actin anterograde flow with phagocytic cup formation, and (iii) the rapid growth of new protrusions. To explore the role of filopodia-inducing Cdc42, we generated myeloid-restricted Cdc42 knock-out mice. Cdc42-deficient macrophages exhibited rapid phagocytic cup kinetics, but reduced particle clearance, which could be explained by the marked rounded-up morphology of these cells. Macrophages lacking Myo10, thought to act downstream of Cdc42, had normal morphology, motility, and phagocytic cup formation, but displayed markedly reduced filopodia formation. In conclusion, live-cell imaging revealed multiple mechanisms involving macrophage filopodia in particle capture and engulfment. Cdc42 is not critical for filopodia or phagocytic cup formation, but plays a key role in driving macrophage lamellipodial spreading."}],"month":"04","intvolume":" 292","scopus_import":1,"file":[{"date_created":"2019-10-24T15:25:42Z","file_name":"2017_JBC_Horsthemke.pdf","creator":"dernst","date_updated":"2020-07-14T12:47:37Z","file_size":5647880,"file_id":"6971","checksum":"d488162874326a4bb056065fa549dc4a","access_level":"open_access","relation":"main_file","content_type":"application/pdf"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["00219258"]},"publication_status":"published","volume":292,"issue":"17","_id":"668","status":"public","type":"journal_article","article_type":"original","ddc":["570"],"date_updated":"2021-01-12T08:08:34Z","department":[{"_id":"MiSi"}],"file_date_updated":"2020-07-14T12:47:37Z"}]