---
_id: '6997'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Friml J. Auxin guides roots to avoid obstacles during gravitropic
growth. New Phytologist. 2020;225(3):1049-1052. doi:10.1111/nph.16203
apa: Zhang, Y., & Friml, J. (2020). Auxin guides roots to avoid obstacles during
gravitropic growth. New Phytologist. Wiley. https://doi.org/10.1111/nph.16203
chicago: Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during
Gravitropic Growth.” New Phytologist. Wiley, 2020. https://doi.org/10.1111/nph.16203.
ieee: Y. Zhang and J. Friml, “Auxin guides roots to avoid obstacles during gravitropic
growth,” New Phytologist, vol. 225, no. 3. Wiley, pp. 1049–1052, 2020.
ista: Zhang Y, Friml J. 2020. Auxin guides roots to avoid obstacles during gravitropic
growth. New Phytologist. 225(3), 1049–1052.
mla: Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during
Gravitropic Growth.” New Phytologist, vol. 225, no. 3, Wiley, 2020, pp.
1049–52, doi:10.1111/nph.16203.
short: Y. Zhang, J. Friml, New Phytologist 225 (2020) 1049–1052.
date_created: 2019-11-12T11:41:32Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-17T14:01:49Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.16203
ec_funded: 1
external_id:
isi:
- '000489638800001'
pmid:
- '31603260'
file:
- access_level: open_access
checksum: cd42ffdb381fd52812b9583d4d407139
content_type: application/pdf
creator: dernst
date_created: 2020-11-18T16:42:48Z
date_updated: 2020-11-18T16:42:48Z
file_id: '8772'
file_name: 2020_NewPhytologist_Zhang.pdf
file_size: 717345
relation: main_file
success: 1
file_date_updated: 2020-11-18T16:42:48Z
has_accepted_license: '1'
intvolume: ' 225'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 1049-1052
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin guides roots to avoid obstacles during gravitropic growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 225
year: '2020'
...
---
_id: '7204'
abstract:
- lang: eng
text: Plant root architecture dynamically adapts to various environmental conditions,
such as salt‐containing soil. The phytohormone abscisic acid (ABA) is involved
among others also in these developmental adaptations, but the underlying molecular
mechanism remains elusive. Here, a novel branch of the ABA signaling pathway in
Arabidopsis involving PYR/PYL/RCAR (abbreviated as PYLs) receptor‐protein phosphatase
2A (PP2A) complex that acts in parallel to the canonical PYLs‐protein phosphatase
2C (PP2C) mechanism is identified. The PYLs‐PP2A signaling modulates root gravitropism
and lateral root formation through regulating phytohormone auxin transport. In
optimal conditions, PYLs ABA receptor interacts with the catalytic subunits of
PP2A, increasing their phosphatase activity and thus counteracting PINOID (PID)
kinase‐mediated phosphorylation of PIN‐FORMED (PIN) auxin transporters. By contrast,
in salt and osmotic stress conditions, ABA binds to PYLs, inhibiting the PP2A
activity, which leads to increased PIN phosphorylation and consequently modulated
directional auxin transport leading to adapted root architecture. This work reveals
an adaptive mechanism that may flexibly adjust plant root growth to withstand
saline and osmotic stresses. It occurs via the cross‐talk between the stress hormone
ABA and the versatile developmental regulator auxin.
article_number: '1901455'
article_processing_charge: No
article_type: original
author:
- first_name: Yang
full_name: Li, Yang
last_name: Li
- first_name: Yaping
full_name: Wang, Yaping
last_name: Wang
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Zhen
full_name: Li, Zhen
last_name: Li
- first_name: Zhi
full_name: Yuan, Zhi
last_name: Yuan
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: David
full_name: Domjan, David
id: C684CD7A-257E-11EA-9B6F-D8588B4F947F
last_name: Domjan
orcid: 0000-0003-2267-106X
- first_name: Kai
full_name: Wang, Kai
last_name: Wang
- first_name: Wei
full_name: Xuan, Wei
last_name: Xuan
- first_name: Yan
full_name: Guo, Yan
last_name: Guo
- first_name: Zhizhong
full_name: Gong, Zhizhong
last_name: Gong
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jing
full_name: Zhang, Jing
last_name: Zhang
citation:
ama: Li Y, Wang Y, Tan S, et al. Root growth adaptation is mediated by PYLs ABA
receptor-PP2A protein phosphatase complex. Advanced Science. 2020;7(3).
doi:10.1002/advs.201901455
apa: Li, Y., Wang, Y., Tan, S., Li, Z., Yuan, Z., Glanc, M., … Zhang, J. (2020).
Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase
complex. Advanced Science. Wiley. https://doi.org/10.1002/advs.201901455
chicago: Li, Yang, Yaping Wang, Shutang Tan, Zhen Li, Zhi Yuan, Matous Glanc, David
Domjan, et al. “Root Growth Adaptation Is Mediated by PYLs ABA Receptor-PP2A Protein
Phosphatase Complex.” Advanced Science. Wiley, 2020. https://doi.org/10.1002/advs.201901455.
ieee: Y. Li et al., “Root growth adaptation is mediated by PYLs ABA receptor-PP2A
protein phosphatase complex,” Advanced Science, vol. 7, no. 3. Wiley, 2020.
ista: Li Y, Wang Y, Tan S, Li Z, Yuan Z, Glanc M, Domjan D, Wang K, Xuan W, Guo
Y, Gong Z, Friml J, Zhang J. 2020. Root growth adaptation is mediated by PYLs
ABA receptor-PP2A protein phosphatase complex. Advanced Science. 7(3), 1901455.
mla: Li, Yang, et al. “Root Growth Adaptation Is Mediated by PYLs ABA Receptor-PP2A
Protein Phosphatase Complex.” Advanced Science, vol. 7, no. 3, 1901455,
Wiley, 2020, doi:10.1002/advs.201901455.
short: Y. Li, Y. Wang, S. Tan, Z. Li, Z. Yuan, M. Glanc, D. Domjan, K. Wang, W.
Xuan, Y. Guo, Z. Gong, J. Friml, J. Zhang, Advanced Science 7 (2020).
date_created: 2019-12-22T23:00:43Z
date_published: 2020-02-05T00:00:00Z
date_updated: 2023-08-17T14:13:17Z
day: '05'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1002/advs.201901455
external_id:
isi:
- '000501912800001'
pmid:
- '32042554'
file:
- access_level: open_access
checksum: 016eeab5860860af038e2da95ffe75c3
content_type: application/pdf
creator: dernst
date_created: 2020-02-24T14:29:54Z
date_updated: 2020-07-14T12:47:53Z
file_id: '7519'
file_name: 2020_AdvScience_Li.pdf
file_size: 3586924
relation: main_file
file_date_updated: 2020-07-14T12:47:53Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
publication: Advanced Science
publication_identifier:
eissn:
- 2198-3844
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root growth adaptation is mediated by PYLs ABA receptor-PP2A protein phosphatase
complex
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 7
year: '2020'
...
---
_id: '7142'
abstract:
- lang: eng
text: The phytohormone auxin acts as an amazingly versatile coordinator of plant
growth and development. With its morphogen-like properties, auxin controls sites
and timing of differentiation and/or growth responses both, in quantitative and
qualitative terms. Specificity in the auxin response depends largely on distinct
modes of signal transmission, by which individual cells perceive and convert auxin
signals into a remarkable diversity of responses. The best understood, or so-called
canonical mechanism of auxin perception ultimately results in variable adjustments
of the cellular transcriptome, via a short, nuclear signal transduction pathway.
Additional findings that accumulated over decades implied that an additional,
presumably, cell surface-based auxin perception mechanism mediates very rapid
cellular responses and decisively contributes to the cell's overall hormonal response.
Recent investigations into both, nuclear and cell surface auxin signalling challenged
this assumed partition of roles for different auxin signalling pathways and revealed
an unexpected complexity in transcriptional and non-transcriptional cellular responses
mediated by auxin.
acknowledgement: Research in J.F. laboratory is funded by the European Union's Horizon
2020 program (ERC grant agreement n° 742985); C.L. is supported by the Austrian
Science Fund (FWF grant P 31493).
article_processing_charge: No
article_type: original
author:
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Gallei MC, Luschnig C, Friml J. Auxin signalling in growth: Schrödinger’s
cat out of the bag. Current Opinion in Plant Biology. 2020;53(2):43-49.
doi:10.1016/j.pbi.2019.10.003'
apa: 'Gallei, M. C., Luschnig, C., & Friml, J. (2020). Auxin signalling in growth:
Schrödinger’s cat out of the bag. Current Opinion in Plant Biology. Elsevier.
https://doi.org/10.1016/j.pbi.2019.10.003'
chicago: 'Gallei, Michelle C, Christian Luschnig, and Jiří Friml. “Auxin Signalling
in Growth: Schrödinger’s Cat out of the Bag.” Current Opinion in Plant Biology.
Elsevier, 2020. https://doi.org/10.1016/j.pbi.2019.10.003.'
ieee: 'M. C. Gallei, C. Luschnig, and J. Friml, “Auxin signalling in growth: Schrödinger’s
cat out of the bag,” Current Opinion in Plant Biology, vol. 53, no. 2.
Elsevier, pp. 43–49, 2020.'
ista: 'Gallei MC, Luschnig C, Friml J. 2020. Auxin signalling in growth: Schrödinger’s
cat out of the bag. Current Opinion in Plant Biology. 53(2), 43–49.'
mla: 'Gallei, Michelle C., et al. “Auxin Signalling in Growth: Schrödinger’s Cat
out of the Bag.” Current Opinion in Plant Biology, vol. 53, no. 2, Elsevier,
2020, pp. 43–49, doi:10.1016/j.pbi.2019.10.003.'
short: M.C. Gallei, C. Luschnig, J. Friml, Current Opinion in Plant Biology 53 (2020)
43–49.
date_created: 2019-12-02T12:05:26Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-17T14:07:22Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2019.10.003
ec_funded: 1
external_id:
isi:
- '000521120600007'
pmid:
- '31760231'
intvolume: ' 53'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 43-49
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Current Opinion in Plant Biology
publication_identifier:
eissn:
- 1879-0356
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 'Auxin signalling in growth: Schrödinger''s cat out of the bag'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 53
year: '2020'
...
---
_id: '7219'
abstract:
- lang: eng
text: Root system architecture (RSA), governed by the phytohormone auxin, endows
plants with an adaptive advantage in particular environments. Using geographically
representative arabidopsis (Arabidopsis thaliana) accessions as a resource for
GWA mapping, Waidmann et al. and Ogura et al. recently identified two novel components
involved in modulating auxin-mediated RSA and conferring plant fitness in particular
habitats.
article_processing_charge: No
article_type: original
author:
- first_name: Guanghui
full_name: Xiao, Guanghui
last_name: Xiao
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
citation:
ama: 'Xiao G, Zhang Y. Adaptive growth: Shaping auxin-mediated root system architecture.
Trends in Plant Science. 2020;25(2):P121-123. doi:10.1016/j.tplants.2019.12.001'
apa: 'Xiao, G., & Zhang, Y. (2020). Adaptive growth: Shaping auxin-mediated
root system architecture. Trends in Plant Science. Elsevier. https://doi.org/10.1016/j.tplants.2019.12.001'
chicago: 'Xiao, Guanghui, and Yuzhou Zhang. “Adaptive Growth: Shaping Auxin-Mediated
Root System Architecture.” Trends in Plant Science. Elsevier, 2020. https://doi.org/10.1016/j.tplants.2019.12.001.'
ieee: 'G. Xiao and Y. Zhang, “Adaptive growth: Shaping auxin-mediated root system
architecture,” Trends in Plant Science, vol. 25, no. 2. Elsevier, pp. P121-123,
2020.'
ista: 'Xiao G, Zhang Y. 2020. Adaptive growth: Shaping auxin-mediated root system
architecture. Trends in Plant Science. 25(2), P121-123.'
mla: 'Xiao, Guanghui, and Yuzhou Zhang. “Adaptive Growth: Shaping Auxin-Mediated
Root System Architecture.” Trends in Plant Science, vol. 25, no. 2, Elsevier,
2020, pp. P121-123, doi:10.1016/j.tplants.2019.12.001.'
short: G. Xiao, Y. Zhang, Trends in Plant Science 25 (2020) P121-123.
date_created: 2019-12-29T23:00:48Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-17T14:14:50Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.tplants.2019.12.001
external_id:
isi:
- '000508637500001'
pmid:
- '31843370'
intvolume: ' 25'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: P121-123
pmid: 1
publication: Trends in Plant Science
publication_identifier:
issn:
- '13601385'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Adaptive growth: Shaping auxin-mediated root system architecture'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 25
year: '2020'
...
---
_id: '7465'
abstract:
- lang: eng
text: The flexible development of plants is characterized by a high capacity for
post-embryonic organ formation and tissue regeneration, processes, which require
tightly regulated intercellular communication and coordinated tissue (re-)polarization.
The phytohormone auxin, the main driver for these processes, is able to establish
polarized auxin transport channels, which are characterized by the expression
and polar, subcellular localization of the PIN1 auxin transport proteins. These
channels are demarcating the position of future vascular strands necessary for
organ formation and tissue regeneration. Major progress has been made in the last
years to understand how PINs can change their polarity in different contexts and
thus guide auxin flow through the plant. However, it still remains elusive how
auxin mediates the establishment of auxin conducting channels and the formation
of vascular tissue and which cellular processes are involved. By the means of
sophisticated regeneration experiments combined with local auxin applications
in Arabidopsis thaliana inflorescence stems we show that (i) PIN subcellular dynamics,
(ii) PIN internalization by clathrin-mediated trafficking and (iii) an intact
actin cytoskeleton required for post-endocytic trafficking are indispensable for
auxin channel formation, de novo vascular formation and vascular regeneration
after wounding. These observations provide novel insights into cellular mechanism
of coordinated tissue polarization during auxin canalization.
article_number: '110414'
article_processing_charge: No
article_type: original
author:
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Hélène S.
full_name: Robert, Hélène S.
last_name: Robert
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. Clathrin-mediated
trafficking and PIN trafficking are required for auxin canalization and vascular
tissue formation in Arabidopsis. Plant Science. 2020;293(4). doi:10.1016/j.plantsci.2020.110414
apa: Mazur, E., Gallei, M. C., Adamowski, M., Han, H., Robert, H. S., & Friml,
J. (2020). Clathrin-mediated trafficking and PIN trafficking are required for
auxin canalization and vascular tissue formation in Arabidopsis. Plant Science.
Elsevier. https://doi.org/10.1016/j.plantsci.2020.110414
chicago: Mazur, Ewa, Michelle C Gallei, Maciek Adamowski, Huibin Han, Hélène S.
Robert, and Jiří Friml. “Clathrin-Mediated Trafficking and PIN Trafficking Are
Required for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.”
Plant Science. Elsevier, 2020. https://doi.org/10.1016/j.plantsci.2020.110414.
ieee: E. Mazur, M. C. Gallei, M. Adamowski, H. Han, H. S. Robert, and J. Friml,
“Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
and vascular tissue formation in Arabidopsis,” Plant Science, vol. 293,
no. 4. Elsevier, 2020.
ista: Mazur E, Gallei MC, Adamowski M, Han H, Robert HS, Friml J. 2020. Clathrin-mediated
trafficking and PIN trafficking are required for auxin canalization and vascular
tissue formation in Arabidopsis. Plant Science. 293(4), 110414.
mla: Mazur, Ewa, et al. “Clathrin-Mediated Trafficking and PIN Trafficking Are Required
for Auxin Canalization and Vascular Tissue Formation in Arabidopsis.” Plant
Science, vol. 293, no. 4, 110414, Elsevier, 2020, doi:10.1016/j.plantsci.2020.110414.
short: E. Mazur, M.C. Gallei, M. Adamowski, H. Han, H.S. Robert, J. Friml, Plant
Science 293 (2020).
date_created: 2020-02-09T23:00:50Z
date_published: 2020-04-01T00:00:00Z
date_updated: 2023-08-17T14:37:32Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.plantsci.2020.110414
ec_funded: 1
external_id:
isi:
- '000520609800009'
file:
- access_level: open_access
checksum: f7f27c6a8fea985ceb9279be2204461c
content_type: application/pdf
creator: dernst
date_created: 2020-02-10T08:59:36Z
date_updated: 2020-07-14T12:47:59Z
file_id: '7471'
file_name: 2020_PlantScience_Mazur.pdf
file_size: 3499069
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 293'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Science
publication_identifier:
eissn:
- '18732259'
issn:
- '01689452'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Clathrin-mediated trafficking and PIN trafficking are required for auxin canalization
and vascular tissue formation in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 293
year: '2020'
...
---
_id: '7490'
abstract:
- lang: eng
text: In plants, clathrin mediated endocytosis (CME) represents the major route
for cargo internalisation from the cell surface. It has been assumed to operate
in an evolutionary conserved manner as in yeast and animals. Here we report characterisation
of ultrastructure, dynamics and mechanisms of plant CME as allowed by our advancement
in electron microscopy and quantitative live imaging techniques. Arabidopsis CME
appears to follow the constant curvature model and the bona fide CME population
generates vesicles of a predominantly hexagonal-basket type; larger and with faster
kinetics than in other models. Contrary to the existing paradigm, actin is dispensable
for CME events at the plasma membrane but plays a unique role in collecting endocytic
vesicles, sorting of internalised cargos and directional endosome movement that
itself actively promote CME events. Internalized vesicles display a strongly delayed
and sequential uncoating. These unique features highlight the independent evolution
of the plant CME mechanism during the autonomous rise of multicellularity in eukaryotes.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
article_number: e52067
article_processing_charge: No
article_type: original
author:
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Roshan
full_name: Prizak, Roshan
id: 4456104E-F248-11E8-B48F-1D18A9856A87
last_name: Prizak
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Barbara E
full_name: Casillas Perez, Barbara E
id: 351ED2AA-F248-11E8-B48F-1D18A9856A87
last_name: Casillas Perez
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Narasimhan M, Johnson AJ, Prizak R, et al. Evolutionarily unique mechanistic
framework of clathrin-mediated endocytosis in plants. eLife. 2020;9. doi:10.7554/eLife.52067
apa: Narasimhan, M., Johnson, A. J., Prizak, R., Kaufmann, W., Tan, S., Casillas
Perez, B. E., & Friml, J. (2020). Evolutionarily unique mechanistic framework
of clathrin-mediated endocytosis in plants. ELife. eLife Sciences Publications.
https://doi.org/10.7554/eLife.52067
chicago: Narasimhan, Madhumitha, Alexander J Johnson, Roshan Prizak, Walter Kaufmann,
Shutang Tan, Barbara E Casillas Perez, and Jiří Friml. “Evolutionarily Unique
Mechanistic Framework of Clathrin-Mediated Endocytosis in Plants.” ELife.
eLife Sciences Publications, 2020. https://doi.org/10.7554/eLife.52067.
ieee: M. Narasimhan et al., “Evolutionarily unique mechanistic framework
of clathrin-mediated endocytosis in plants,” eLife, vol. 9. eLife Sciences
Publications, 2020.
ista: Narasimhan M, Johnson AJ, Prizak R, Kaufmann W, Tan S, Casillas Perez BE,
Friml J. 2020. Evolutionarily unique mechanistic framework of clathrin-mediated
endocytosis in plants. eLife. 9, e52067.
mla: Narasimhan, Madhumitha, et al. “Evolutionarily Unique Mechanistic Framework
of Clathrin-Mediated Endocytosis in Plants.” ELife, vol. 9, e52067, eLife
Sciences Publications, 2020, doi:10.7554/eLife.52067.
short: M. Narasimhan, A.J. Johnson, R. Prizak, W. Kaufmann, S. Tan, B.E. Casillas
Perez, J. Friml, ELife 9 (2020).
date_created: 2020-02-16T23:00:50Z
date_published: 2020-01-23T00:00:00Z
date_updated: 2023-08-18T06:33:07Z
day: '23'
ddc:
- '570'
- '580'
department:
- _id: JiFr
- _id: GaTk
- _id: EM-Fac
- _id: SyCr
doi: 10.7554/eLife.52067
ec_funded: 1
external_id:
isi:
- '000514104100001'
pmid:
- '31971511'
file:
- access_level: open_access
checksum: 2052daa4be5019534f3a42f200a09f32
content_type: application/pdf
creator: dernst
date_created: 2020-02-18T07:21:16Z
date_updated: 2020-07-14T12:47:59Z
file_id: '7494'
file_name: 2020_eLife_Narasimhan.pdf
file_size: 7247468
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis
in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7497'
abstract:
- lang: eng
text: Endophytic fungi can be beneficial to plant growth. However, the molecular
mechanisms underlying colonization of Acremonium spp. remain unclear. In this
study, a novel endophytic Acremonium strain was isolated from the buds of Panax
notoginseng and named Acremonium sp. D212. The Acremonium sp. D212 could colonize
the roots of P. notoginseng, enhance the resistance of P. notoginseng to root
rot disease, and promote root growth and saponin biosynthesis in P. notoginseng.
Acremonium sp. D212 could secrete indole‐3‐acetic acid (IAA) and jasmonic acid
(JA), and inoculation with the fungus increased the endogenous levels of IAA and
JA in P. notoginseng. Colonization of the Acremonium sp. D212 in the roots of
the rice line Nipponbare was dependent on the concentration of methyl jasmonate
(MeJA) (2 to 15 μM) and 1‐naphthalenacetic acid (NAA) (10 to 20 μM). Moreover,
the roots of the JA signalling‐defective coi1‐18 mutant were colonized by Acremonium
sp. D212 to a lesser degree than those of the wild‐type Nipponbare and miR393b‐overexpressing
lines, and the colonization was rescued by MeJA but not by NAA. It suggests that
the cross‐talk between JA signalling and the auxin biosynthetic pathway plays
a crucial role in the colonization of Acremonium sp. D212 in host plants.
acknowledgement: We thank Professor Jianqiang Wu (Kunming Institute of Botany, Chinese
Academy of Sciences) for providing generous support with the IAA and JA measurements.
We thank Professor Guohua Xu (Nanjing Agricultural University) for generously providing
the Nipponbare rice expressing DR5::GUS. We thank Professor Muyuan Zhu (Zhejiang
University) for generously providing a rice line expressing 35S::miR393b. We thank
Professor Yinong Yang (Pennsylvania State University) for generously providing the
rice line coi1-18. This work was supported by grants from the National Natural Science
Foundation of China (31660501, 31460453, 31860064 and 31470382), the Major Special
Program for Scientific Research, Education Department of Yunnan Province (ZD2015005),
the Project sponsored by SRF for ROCS, SEM ([2013] 1792), the Major Science and
Technique Programs in Yunnan Province (2016ZF001), the Key Projects of the Applied
Basic Research Plan of Yunnan Province (2017FA018), the National Key R&D Program
of China (2018YFD0201100) and the China Agriculture Research System (CARS-21).
article_processing_charge: No
article_type: original
author:
- first_name: L
full_name: Han, L
last_name: Han
- first_name: X
full_name: Zhou, X
last_name: Zhou
- first_name: Y
full_name: Zhao, Y
last_name: Zhao
- first_name: S
full_name: Zhu, S
last_name: Zhu
- first_name: L
full_name: Wu, L
last_name: Wu
- first_name: Y
full_name: He, Y
last_name: He
- first_name: X
full_name: Ping, X
last_name: Ping
- first_name: X
full_name: Lu, X
last_name: Lu
- first_name: W
full_name: Huang, W
last_name: Huang
- first_name: J
full_name: Qian, J
last_name: Qian
- first_name: L
full_name: Zhang, L
last_name: Zhang
- first_name: X
full_name: Jiang, X
last_name: Jiang
- first_name: D
full_name: Zhu, D
last_name: Zhu
- first_name: C
full_name: Luo, C
last_name: Luo
- first_name: S
full_name: Li, S
last_name: Li
- first_name: Q
full_name: Dong, Q
last_name: Dong
- first_name: Q
full_name: Fu, Q
last_name: Fu
- first_name: K
full_name: Deng, K
last_name: Deng
- first_name: X
full_name: Wang, X
last_name: Wang
- first_name: L
full_name: Wang, L
last_name: Wang
- first_name: S
full_name: Peng, S
last_name: Peng
- first_name: J
full_name: Wu, J
last_name: Wu
- first_name: W
full_name: Li, W
last_name: Li
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Y
full_name: Zhu, Y
last_name: Zhu
- first_name: X
full_name: He, X
last_name: He
- first_name: Y
full_name: Du, Y
last_name: Du
citation:
ama: Han L, Zhou X, Zhao Y, et al. Colonization of endophyte Acremonium sp. D212
in Panax notoginseng and rice mediated by auxin and jasmonic acid. Journal
of Integrative Plant Biology. 2020;62(9):1433-1451. doi:10.1111/jipb.12905
apa: Han, L., Zhou, X., Zhao, Y., Zhu, S., Wu, L., He, Y., … Du, Y. (2020). Colonization
of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin
and jasmonic acid. Journal of Integrative Plant Biology. Wiley. https://doi.org/10.1111/jipb.12905
chicago: Han, L, X Zhou, Y Zhao, S Zhu, L Wu, Y He, X Ping, et al. “Colonization
of Endophyte Acremonium Sp. D212 in Panax Notoginseng and Rice Mediated by Auxin
and Jasmonic Acid.” Journal of Integrative Plant Biology. Wiley, 2020.
https://doi.org/10.1111/jipb.12905.
ieee: L. Han et al., “Colonization of endophyte Acremonium sp. D212 in Panax
notoginseng and rice mediated by auxin and jasmonic acid,” Journal of Integrative
Plant Biology, vol. 62, no. 9. Wiley, pp. 1433–1451, 2020.
ista: Han L, Zhou X, Zhao Y, Zhu S, Wu L, He Y, Ping X, Lu X, Huang W, Qian J, Zhang
L, Jiang X, Zhu D, Luo C, Li S, Dong Q, Fu Q, Deng K, Wang X, Wang L, Peng S,
Wu J, Li W, Friml J, Zhu Y, He X, Du Y. 2020. Colonization of endophyte Acremonium
sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid. Journal
of Integrative Plant Biology. 62(9), 1433–1451.
mla: Han, L., et al. “Colonization of Endophyte Acremonium Sp. D212 in Panax Notoginseng
and Rice Mediated by Auxin and Jasmonic Acid.” Journal of Integrative Plant
Biology, vol. 62, no. 9, Wiley, 2020, pp. 1433–51, doi:10.1111/jipb.12905.
short: L. Han, X. Zhou, Y. Zhao, S. Zhu, L. Wu, Y. He, X. Ping, X. Lu, W. Huang,
J. Qian, L. Zhang, X. Jiang, D. Zhu, C. Luo, S. Li, Q. Dong, Q. Fu, K. Deng, X.
Wang, L. Wang, S. Peng, J. Wu, W. Li, J. Friml, Y. Zhu, X. He, Y. Du, Journal
of Integrative Plant Biology 62 (2020) 1433–1451.
date_created: 2020-02-18T10:02:25Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2023-08-18T06:44:16Z
day: '01'
department:
- _id: JiFr
doi: 10.1111/jipb.12905
external_id:
isi:
- '000515803000001'
pmid:
- '31912615'
intvolume: ' 62'
isi: 1
issue: '9'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1111/jipb.12905
month: '09'
oa: 1
oa_version: Published Version
page: 1433-1451
pmid: 1
publication: Journal of Integrative Plant Biology
publication_identifier:
eissn:
- 1744-7909
issn:
- 1672-9072
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice
mediated by auxin and jasmonic acid
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 62
year: '2020'
...
---
_id: '7540'
abstract:
- lang: eng
text: ' In vitro propagation of the ornamentally interesting species Wikstroemia
gemmata is limited by the recalcitrance to form adventitious roots. In this article,
two strategies to improve the rooting capacity of in vitro microcuttings are presented.
Firstly, the effect of exogenous auxin was evaluated in both light and dark cultivated
stem segments and also the sucrose-content of the medium was varied in order to
determine better rooting conditions. Secondly, different spectral lights were
evaluated and the effect on shoot growth and root induction demonstrated that
the exact spectral composition of light is important for successful in vitro growth
and development of Wikstroemia gemmata. We show that exogenous auxin cannot compensate
for the poor rooting under unfavorable light conditions. Adapting the culture
conditions is therefore paramount for successful industrial propagation of Wikstroemia
gemmata. '
article_processing_charge: No
article_type: original
author:
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: H.
full_name: Buyle, H.
last_name: Buyle
- first_name: S.
full_name: Werbrouck, S.
last_name: Werbrouck
- first_name: M.C.
full_name: Van Labeke, M.C.
last_name: Van Labeke
- first_name: D.
full_name: Geelen, D.
last_name: Geelen
citation:
ama: Verstraeten I, Buyle H, Werbrouck S, Van Labeke MC, Geelen D. In vitro shoot
growth and adventitious rooting of Wikstroemia gemmata depends on light quality.
Israel Journal of Plant Sciences. 2020;67(1-2):16-26. doi:10.1163/22238980-20191110
apa: Verstraeten, I., Buyle, H., Werbrouck, S., Van Labeke, M. C., & Geelen,
D. (2020). In vitro shoot growth and adventitious rooting of Wikstroemia gemmata
depends on light quality. Israel Journal of Plant Sciences. Brill. https://doi.org/10.1163/22238980-20191110
chicago: Verstraeten, Inge, H. Buyle, S. Werbrouck, M.C. Van Labeke, and D. Geelen.
“In Vitro Shoot Growth and Adventitious Rooting of Wikstroemia Gemmata Depends
on Light Quality.” Israel Journal of Plant Sciences. Brill, 2020. https://doi.org/10.1163/22238980-20191110.
ieee: I. Verstraeten, H. Buyle, S. Werbrouck, M. C. Van Labeke, and D. Geelen, “In
vitro shoot growth and adventitious rooting of Wikstroemia gemmata depends on
light quality,” Israel Journal of Plant Sciences, vol. 67, no. 1–2. Brill,
pp. 16–26, 2020.
ista: Verstraeten I, Buyle H, Werbrouck S, Van Labeke MC, Geelen D. 2020. In vitro
shoot growth and adventitious rooting of Wikstroemia gemmata depends on light
quality. Israel Journal of Plant Sciences. 67(1–2), 16–26.
mla: Verstraeten, Inge, et al. “In Vitro Shoot Growth and Adventitious Rooting of
Wikstroemia Gemmata Depends on Light Quality.” Israel Journal of Plant Sciences,
vol. 67, no. 1–2, Brill, 2020, pp. 16–26, doi:10.1163/22238980-20191110.
short: I. Verstraeten, H. Buyle, S. Werbrouck, M.C. Van Labeke, D. Geelen, Israel
Journal of Plant Sciences 67 (2020) 16–26.
date_created: 2020-02-28T09:18:01Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-18T06:45:15Z
day: '01'
department:
- _id: JiFr
doi: 10.1163/22238980-20191110
external_id:
isi:
- '000525343300004'
intvolume: ' 67'
isi: 1
issue: 1-2
language:
- iso: eng
month: '02'
oa_version: None
page: 16-26
publication: Israel Journal of Plant Sciences
publication_identifier:
eissn:
- 2223-8980
issn:
- 0792-9978
publication_status: published
publisher: Brill
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vitro shoot growth and adventitious rooting of Wikstroemia gemmata depends
on light quality
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 67
year: '2020'
...
---
_id: '7582'
abstract:
- lang: eng
text: Small RNAs (smRNA, 19–25 nucleotides long), which are transcribed by RNA polymerase
II, regulate the expression of genes involved in a multitude of processes in eukaryotes.
miRNA biogenesis and the proteins involved in the biogenesis pathway differ across
plant and animal lineages. The major proteins constituting the biogenesis pathway,
namely, the Dicers (DCL/DCR) and Argonautes (AGOs), have been extensively studied.
However, the accessory proteins (DAWDLE (DDL), SERRATE (SE), and TOUGH (TGH))
of the pathway that differs across the two lineages remain largely uncharacterized.
We present the first detailed report on the molecular evolution and divergence
of these proteins across eukaryotes. Although DDL is present in eukaryotes and
prokaryotes, SE and TGH appear to be specific to eukaryotes. The addition/deletion
of specific domains and/or domain-specific sequence divergence in the three proteins
points to the observed functional divergence of these proteins across the two
lineages, which correlates with the differences in miRNA length across the two
lineages. Our data enhance the current understanding of the structure–function
relationship of these proteins and reveals previous unexplored crucial residues
in the three proteins that can be used as a basis for further functional characterization.
The data presented here on the number of miRNAs in crown eukaryotic lineages are
consistent with the notion of the expansion of the number of miRNA-coding genes
in animal and plant lineages correlating with organismal complexity. Whether this
difference in functionally correlates with the diversification (or presence/absence)
of the three proteins studied here or the miRNA signaling in the plant and animal
lineages is unclear. Based on our results of the three proteins studied here and
previously available data concerning the evolution of miRNA genes in the plant
and animal lineages, we believe that miRNAs probably evolved once in the ancestor
to crown eukaryotes and have diversified independently in the eukaryotes.
article_number: '299'
article_processing_charge: No
article_type: original
author:
- first_name: Taraka Ramji
full_name: Moturu, Taraka Ramji
last_name: Moturu
- first_name: Sansrity
full_name: Sinha, Sansrity
last_name: Sinha
- first_name: Hymavathi
full_name: Salava, Hymavathi
last_name: Salava
- first_name: Sravankumar
full_name: Thula, Sravankumar
last_name: Thula
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Radka Svobodová
full_name: Vařeková, Radka Svobodová
last_name: Vařeková
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
citation:
ama: Moturu TR, Sinha S, Salava H, et al. Molecular evolution and diversification
of proteins involved in miRNA maturation pathway. Plants. 2020;9(3). doi:10.3390/plants9030299
apa: Moturu, T. R., Sinha, S., Salava, H., Thula, S., Nodzyński, T., Vařeková, R.
S., … Simon, S. (2020). Molecular evolution and diversification of proteins involved
in miRNA maturation pathway. Plants. MDPI. https://doi.org/10.3390/plants9030299
chicago: Moturu, Taraka Ramji, Sansrity Sinha, Hymavathi Salava, Sravankumar Thula,
Tomasz Nodzyński, Radka Svobodová Vařeková, Jiří Friml, and Sibu Simon. “Molecular
Evolution and Diversification of Proteins Involved in MiRNA Maturation Pathway.”
Plants. MDPI, 2020. https://doi.org/10.3390/plants9030299.
ieee: T. R. Moturu et al., “Molecular evolution and diversification of proteins
involved in miRNA maturation pathway,” Plants, vol. 9, no. 3. MDPI, 2020.
ista: Moturu TR, Sinha S, Salava H, Thula S, Nodzyński T, Vařeková RS, Friml J,
Simon S. 2020. Molecular evolution and diversification of proteins involved in
miRNA maturation pathway. Plants. 9(3), 299.
mla: Moturu, Taraka Ramji, et al. “Molecular Evolution and Diversification of Proteins
Involved in MiRNA Maturation Pathway.” Plants, vol. 9, no. 3, 299, MDPI,
2020, doi:10.3390/plants9030299.
short: T.R. Moturu, S. Sinha, H. Salava, S. Thula, T. Nodzyński, R.S. Vařeková,
J. Friml, S. Simon, Plants 9 (2020).
date_created: 2020-03-15T23:00:52Z
date_published: 2020-03-01T00:00:00Z
date_updated: 2023-08-18T07:07:08Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/plants9030299
ec_funded: 1
external_id:
isi:
- '000525315000035'
pmid:
- '32121542'
file:
- access_level: open_access
checksum: 6d5af3e17266a48996b4af4e67e88a85
content_type: application/pdf
creator: dernst
date_created: 2020-03-23T13:37:00Z
date_updated: 2020-07-14T12:48:00Z
file_id: '7614'
file_name: 2020_Plants_Moturu.pdf
file_size: 2373484
relation: main_file
file_date_updated: 2020-07-14T12:48:00Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Plants
publication_identifier:
eissn:
- '22237747'
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular evolution and diversification of proteins involved in miRNA maturation
pathway
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7600'
abstract:
- lang: eng
text: Directional intercellular transport of the phytohormone auxin mediated by
PIN FORMED (PIN) efflux carriers plays essential roles in both coordinating patterning
processes and integrating multiple external cues by rapidly redirecting auxin
fluxes. Multilevel regulations of PIN activity under internal and external cues
are complicated; however, the underlying molecular mechanism remains elusive.
Here we demonstrate that 3’-Phosphoinositide-Dependent Protein Kinase1 (PDK1),
which is conserved in plants and mammals, functions as a molecular hub integrating
the upstream lipid signalling and the downstream substrate activity through phosphorylation.
Genetic analysis uncovers that loss-of-function Arabidopsis mutant pdk1.1 pdk1.2
exhibits a plethora of abnormalities in organogenesis and growth, due to the defective
PIN-dependent auxin transport. Further cellular and biochemical analyses reveal
that PDK1 phosphorylates D6 Protein Kinase to facilitate its activity towards
PIN proteins. Our studies establish a lipid-dependent phosphorylation cascade
connecting membrane composition-based cellular signalling with plant growth and
patterning by regulating morphogenetic auxin fluxes.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
article_processing_charge: No
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Wei
full_name: Kong, Wei
last_name: Kong
- first_name: Xiao-Li
full_name: Yang, Xiao-Li
last_name: Yang
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Zuzana
full_name: Vondráková, Zuzana
last_name: Vondráková
- first_name: Roberta
full_name: Filepová, Roberta
last_name: Filepová
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Hong-Wei
full_name: Xue, Hong-Wei
last_name: Xue
citation:
ama: Tan S, Zhang X, Kong W, et al. The lipid code-dependent phosphoswitch PDK1–D6PK
activates PIN-mediated auxin efflux in Arabidopsis. Nature Plants. 2020;6:556-569.
doi:10.1038/s41477-020-0648-9
apa: Tan, S., Zhang, X., Kong, W., Yang, X.-L., Molnar, G., Vondráková, Z., … Xue,
H.-W. (2020). The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated
auxin efflux in Arabidopsis. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-020-0648-9
chicago: Tan, Shutang, Xixi Zhang, Wei Kong, Xiao-Li Yang, Gergely Molnar, Zuzana
Vondráková, Roberta Filepová, Jan Petrášek, Jiří Friml, and Hong-Wei Xue. “The
Lipid Code-Dependent Phosphoswitch PDK1–D6PK Activates PIN-Mediated Auxin Efflux
in Arabidopsis.” Nature Plants. Springer Nature, 2020. https://doi.org/10.1038/s41477-020-0648-9.
ieee: S. Tan et al., “The lipid code-dependent phosphoswitch PDK1–D6PK activates
PIN-mediated auxin efflux in Arabidopsis,” Nature Plants, vol. 6. Springer
Nature, pp. 556–569, 2020.
ista: Tan S, Zhang X, Kong W, Yang X-L, Molnar G, Vondráková Z, Filepová R, Petrášek
J, Friml J, Xue H-W. 2020. The lipid code-dependent phosphoswitch PDK1–D6PK activates
PIN-mediated auxin efflux in Arabidopsis. Nature Plants. 6, 556–569.
mla: Tan, Shutang, et al. “The Lipid Code-Dependent Phosphoswitch PDK1–D6PK Activates
PIN-Mediated Auxin Efflux in Arabidopsis.” Nature Plants, vol. 6, Springer
Nature, 2020, pp. 556–69, doi:10.1038/s41477-020-0648-9.
short: S. Tan, X. Zhang, W. Kong, X.-L. Yang, G. Molnar, Z. Vondráková, R. Filepová,
J. Petrášek, J. Friml, H.-W. Xue, Nature Plants 6 (2020) 556–569.
date_created: 2020-03-21T16:34:16Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2023-08-18T07:05:57Z
day: '01'
department:
- _id: JiFr
doi: 10.1038/s41477-020-0648-9
ec_funded: 1
external_id:
isi:
- '000531787500006'
pmid:
- '32393881'
intvolume: ' 6'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/755504
month: '05'
oa: 1
oa_version: Preprint
page: 556-569
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
grant_number: 723-2015
name: Long Term Fellowship
publication: Nature Plants
publication_identifier:
eissn:
- '20550278'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41477-020-0719-y
scopus_import: '1'
status: public
title: The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin
efflux in Arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 6
year: '2020'
...
---
_id: '7646'
abstract:
- lang: eng
text: In plant cells, environmental stressors promote changes in connectivity between
the cortical ER and the PM. Although this process is tightly regulated in space
and time, the molecular signals and structural components mediating these changes
in inter-organelle communication are only starting to be characterized. In this
report, we confirm the presence of a putative tethering complex containing the
synaptotagmins 1 and 5 (SYT1 and SYT5) and the Ca2+ and lipid binding protein
1 (CLB1/SYT7). This complex is enriched at ER-PM contact sites (EPCS), have slow
responses to changes in extracellular Ca2+, and display severe cytoskeleton-dependent
rearrangements in response to the trivalent lanthanum (La3+) and gadolinium (Gd3+)
rare earth elements (REEs). Although REEs are generally used as non-selective
cation channel blockers at the PM, here we show that the slow internalization
of REEs into the cytosol underlies the activation of the Ca2+/Calmodulin intracellular
signaling, the accumulation of phosphatidylinositol-4-phosphate (PI4P) at the
PM, and the cytoskeleton-dependent rearrangement of the SYT1/SYT5 EPCS complexes.
We propose that the observed EPCS rearrangements act as a slow adaptive response
to sustained stress conditions, and that this process involves the accumulation
of stress-specific phosphoinositides species at the PM.
article_processing_charge: No
article_type: original
author:
- first_name: E
full_name: Lee, E
last_name: Lee
- first_name: B
full_name: Vila Nova Santana, B
last_name: Vila Nova Santana
- first_name: E
full_name: Samuels, E
last_name: Samuels
- first_name: F
full_name: Benitez-Fuente, F
last_name: Benitez-Fuente
- first_name: E
full_name: Corsi, E
last_name: Corsi
- first_name: MA
full_name: Botella, MA
last_name: Botella
- first_name: J
full_name: Perez-Sancho, J
last_name: Perez-Sancho
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: A
full_name: Macho, A
last_name: Macho
- first_name: A
full_name: Alves Azevedo, A
last_name: Alves Azevedo
- first_name: A
full_name: Rosado, A
last_name: Rosado
citation:
ama: Lee E, Vila Nova Santana B, Samuels E, et al. Rare earth elements induce cytoskeleton-dependent
and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in
Arabidopsis. Journal of Experimental Botany. 2020;71(14):3986–3998. doi:10.1093/jxb/eraa138
apa: Lee, E., Vila Nova Santana, B., Samuels, E., Benitez-Fuente, F., Corsi, E.,
Botella, M., … Rosado, A. (2020). Rare earth elements induce cytoskeleton-dependent
and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in
Arabidopsis. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/eraa138
chicago: Lee, E, B Vila Nova Santana, E Samuels, F Benitez-Fuente, E Corsi, MA Botella,
J Perez-Sancho, et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and
PI4P-Associated Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.”
Journal of Experimental Botany. Oxford University Press, 2020. https://doi.org/10.1093/jxb/eraa138.
ieee: E. Lee et al., “Rare earth elements induce cytoskeleton-dependent and
PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis,”
Journal of Experimental Botany, vol. 71, no. 14. Oxford University Press,
pp. 3986–3998, 2020.
ista: Lee E, Vila Nova Santana B, Samuels E, Benitez-Fuente F, Corsi E, Botella
M, Perez-Sancho J, Vanneste S, Friml J, Macho A, Alves Azevedo A, Rosado A. 2020.
Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement
of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. Journal of Experimental
Botany. 71(14), 3986–3998.
mla: Lee, E., et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and PI4P-Associated
Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.” Journal
of Experimental Botany, vol. 71, no. 14, Oxford University Press, 2020, pp.
3986–3998, doi:10.1093/jxb/eraa138.
short: E. Lee, B. Vila Nova Santana, E. Samuels, F. Benitez-Fuente, E. Corsi, M.
Botella, J. Perez-Sancho, S. Vanneste, J. Friml, A. Macho, A. Alves Azevedo, A.
Rosado, Journal of Experimental Botany 71 (2020) 3986–3998.
date_created: 2020-04-06T10:57:08Z
date_published: 2020-07-06T00:00:00Z
date_updated: 2023-08-18T10:27:52Z
day: '06'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/jxb/eraa138
external_id:
isi:
- '000553125400007'
pmid:
- '32179893'
file:
- access_level: open_access
checksum: b06aaaa93dc41896da805fe4b75cf3a1
content_type: application/pdf
creator: dernst
date_created: 2020-10-06T07:41:35Z
date_updated: 2020-10-06T07:41:35Z
file_id: '8613'
file_name: 2020_JourExperimBotany_Lee.pdf
file_size: 1916031
relation: main_file
success: 1
file_date_updated: 2020-10-06T07:41:35Z
has_accepted_license: '1'
intvolume: ' 71'
isi: 1
issue: '14'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 3986–3998
pmid: 1
publication: Journal of Experimental Botany
publication_identifier:
eissn:
- 1460-2431
issn:
- 0022-0957
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
status: public
title: Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement
of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 71
year: '2020'
...
---
_id: '7686'
abstract:
- lang: eng
text: 'The agricultural green revolution spectacularly enhanced crop yield and lodging
resistance with modified DELLA-mediated gibberellin signaling. However, this was
achieved at the expense of reduced nitrogen-use efficiency (NUE). Recently, Wu
et al. revealed novel gibberellin signaling that provides a blueprint for improving
tillering and NUE in Green Revolution varieties (GRVs). '
article_processing_charge: No
article_type: original
author:
- first_name: Huidan
full_name: Xue, Huidan
last_name: Xue
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Guanghui
full_name: Xiao, Guanghui
last_name: Xiao
citation:
ama: 'Xue H, Zhang Y, Xiao G. Neo-gibberellin signaling: Guiding the next generation
of the green revolution. Trends in Plant Science. 2020;25(6):520-522. doi:10.1016/j.tplants.2020.04.001'
apa: 'Xue, H., Zhang, Y., & Xiao, G. (2020). Neo-gibberellin signaling: Guiding
the next generation of the green revolution. Trends in Plant Science. Elsevier.
https://doi.org/10.1016/j.tplants.2020.04.001'
chicago: 'Xue, Huidan, Yuzhou Zhang, and Guanghui Xiao. “Neo-Gibberellin Signaling:
Guiding the next Generation of the Green Revolution.” Trends in Plant Science.
Elsevier, 2020. https://doi.org/10.1016/j.tplants.2020.04.001.'
ieee: 'H. Xue, Y. Zhang, and G. Xiao, “Neo-gibberellin signaling: Guiding the next
generation of the green revolution,” Trends in Plant Science, vol. 25,
no. 6. Elsevier, pp. 520–522, 2020.'
ista: 'Xue H, Zhang Y, Xiao G. 2020. Neo-gibberellin signaling: Guiding the next
generation of the green revolution. Trends in Plant Science. 25(6), 520–522.'
mla: 'Xue, Huidan, et al. “Neo-Gibberellin Signaling: Guiding the next Generation
of the Green Revolution.” Trends in Plant Science, vol. 25, no. 6, Elsevier,
2020, pp. 520–22, doi:10.1016/j.tplants.2020.04.001.'
short: H. Xue, Y. Zhang, G. Xiao, Trends in Plant Science 25 (2020) 520–522.
date_created: 2020-04-26T22:00:46Z
date_published: 2020-06-01T00:00:00Z
date_updated: 2023-08-21T06:16:01Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.tplants.2020.04.001
external_id:
isi:
- '000533518400003'
pmid:
- '32407691'
intvolume: ' 25'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa_version: None
page: 520-522
pmid: 1
publication: Trends in Plant Science
publication_identifier:
issn:
- 1360-1385
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Neo-gibberellin signaling: Guiding the next generation of the green revolution'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 25
year: '2020'
...
---
_id: '7793'
abstract:
- lang: eng
text: Hormonal signalling in animals often involves direct transcription factor-hormone
interactions that modulate gene expression. In contrast, plant hormone signalling
is most commonly based on de-repression via the degradation of transcriptional
repressors. Recently, we uncovered a non-canonical signalling mechanism for the
plant hormone auxin whereby auxin directly affects the activity of the atypical
auxin response factor (ARF), ETTIN towards target genes without the requirement
for protein degradation. Here we show that ETTIN directly binds auxin, leading
to dissociation from co-repressor proteins of the TOPLESS/TOPLESS-RELATED family
followed by histone acetylation and induction of gene expression. This mechanism
is reminiscent of animal hormone signalling as it affects the activity towards
regulation of target genes and provides the first example of a DNA-bound hormone
receptor in plants. Whilst auxin affects canonical ARFs indirectly by facilitating
degradation of Aux/IAA repressors, direct ETTIN-auxin interactions allow switching
between repressive and de-repressive chromatin states in an instantly-reversible
manner.
article_number: e51787
article_processing_charge: No
article_type: original
author:
- first_name: André
full_name: Kuhn, André
last_name: Kuhn
- first_name: Sigurd
full_name: Ramans Harborough, Sigurd
last_name: Ramans Harborough
- first_name: Heather M
full_name: McLaughlin, Heather M
last_name: McLaughlin
- first_name: Bhavani
full_name: Natarajan, Bhavani
last_name: Natarajan
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Stefan
full_name: Kepinski, Stefan
last_name: Kepinski
- first_name: Lars
full_name: Østergaard, Lars
last_name: Østergaard
citation:
ama: Kuhn A, Ramans Harborough S, McLaughlin HM, et al. Direct ETTIN-auxin interaction
controls chromatin states in gynoecium development. eLife. 2020;9. doi:10.7554/elife.51787
apa: Kuhn, A., Ramans Harborough, S., McLaughlin, H. M., Natarajan, B., Verstraeten,
I., Friml, J., … Østergaard, L. (2020). Direct ETTIN-auxin interaction controls
chromatin states in gynoecium development. ELife. eLife Sciences Publications.
https://doi.org/10.7554/elife.51787
chicago: Kuhn, André, Sigurd Ramans Harborough, Heather M McLaughlin, Bhavani Natarajan,
Inge Verstraeten, Jiří Friml, Stefan Kepinski, and Lars Østergaard. “Direct ETTIN-Auxin
Interaction Controls Chromatin States in Gynoecium Development.” ELife.
eLife Sciences Publications, 2020. https://doi.org/10.7554/elife.51787.
ieee: A. Kuhn et al., “Direct ETTIN-auxin interaction controls chromatin
states in gynoecium development,” eLife, vol. 9. eLife Sciences Publications,
2020.
ista: Kuhn A, Ramans Harborough S, McLaughlin HM, Natarajan B, Verstraeten I, Friml
J, Kepinski S, Østergaard L. 2020. Direct ETTIN-auxin interaction controls chromatin
states in gynoecium development. eLife. 9, e51787.
mla: Kuhn, André, et al. “Direct ETTIN-Auxin Interaction Controls Chromatin States
in Gynoecium Development.” ELife, vol. 9, e51787, eLife Sciences Publications,
2020, doi:10.7554/elife.51787.
short: A. Kuhn, S. Ramans Harborough, H.M. McLaughlin, B. Natarajan, I. Verstraeten,
J. Friml, S. Kepinski, L. Østergaard, ELife 9 (2020).
date_created: 2020-05-04T08:50:47Z
date_published: 2020-04-08T00:00:00Z
date_updated: 2023-08-21T06:17:12Z
day: '08'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.7554/elife.51787
external_id:
isi:
- '000527752200001'
pmid:
- '32267233'
file:
- access_level: open_access
checksum: 15d740de1a741fdcc6ec128c48eed017
content_type: application/pdf
creator: dernst
date_created: 2020-05-04T09:06:43Z
date_updated: 2020-07-14T12:48:03Z
file_id: '7794'
file_name: 2020_eLife_Kuhn.pdf
file_size: 2893082
relation: main_file
file_date_updated: 2020-07-14T12:48:03Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Direct ETTIN-auxin interaction controls chromatin states in gynoecium development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '8138'
abstract:
- lang: eng
text: Directional transport of the phytohormone auxin is a versatile, plant-specific
mechanism regulating many aspects of plant development. The recently identified
plant hormones, strigolactones (SLs), are implicated in many plant traits; among
others, they modify the phenotypic output of PIN-FORMED (PIN) auxin transporters
for fine-tuning of growth and developmental responses. Here, we show in pea and
Arabidopsis that SLs target processes dependent on the canalization of auxin flow,
which involves auxin feedback on PIN subcellular distribution. D14 receptor- and
MAX2 F-box-mediated SL signaling inhibits the formation of auxin-conducting channels
after wounding or from artificial auxin sources, during vasculature de novo formation
and regeneration. At the cellular level, SLs interfere with auxin effects on PIN
polar targeting, constitutive PIN trafficking as well as clathrin-mediated endocytosis.
Our results identify a non-transcriptional mechanism of SL action, uncoupling
auxin feedback on PIN polarity and trafficking, thereby regulating vascular tissue
formation and regeneration.
acknowledgement: We are grateful to David Nelson for providing published materials
and extremely helpful comments, and Elizabeth Dun and Christine Beveridge for helpful
discussions. The research leading to these results has received funding from the
European Research Council (ERC) under the European Union's Horizon 2020 research
and innovation programme (742985). This work was also supported by the Beijing Municipal
Natural Science Foundation (5192011), Beijing Outstanding University Discipline
Program, the National Natural Science Foundation of China (31370309), CEITEC 2020
(LQ1601) project with financial contribution made by the Ministry of Education,
Youth and Sports of the Czech Republic within special support paid from the National
Program of Sustainability II funds, Australian Research Council (FT180100081), and
China Postdoctoral Science Foundation (2019M660864).
article_processing_charge: No
article_type: original
author:
- first_name: J
full_name: Zhang, J
last_name: Zhang
- first_name: E
full_name: Mazur, E
last_name: Mazur
- first_name: J
full_name: Balla, J
last_name: Balla
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: P
full_name: Kalousek, P
last_name: Kalousek
- first_name: Z
full_name: Medveďová, Z
last_name: Medveďová
- first_name: Y
full_name: Li, Y
last_name: Li
- first_name: Y
full_name: Wang, Y
last_name: Wang
- first_name: Tomas
full_name: Prat, Tomas
id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
last_name: Prat
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
- first_name: V
full_name: Reinöhl, V
last_name: Reinöhl
- first_name: S
full_name: Procházka, S
last_name: Procházka
- first_name: R
full_name: Halouzka, R
last_name: Halouzka
- first_name: P
full_name: Tarkowski, P
last_name: Tarkowski
- first_name: C
full_name: Luschnig, C
last_name: Luschnig
- first_name: PB
full_name: Brewer, PB
last_name: Brewer
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang J, Mazur E, Balla J, et al. Strigolactones inhibit auxin feedback on
PIN-dependent auxin transport canalization. Nature Communications. 2020;11(1):3508.
doi:10.1038/s41467-020-17252-y
apa: Zhang, J., Mazur, E., Balla, J., Gallei, M. C., Kalousek, P., Medveďová, Z.,
… Friml, J. (2020). Strigolactones inhibit auxin feedback on PIN-dependent auxin
transport canalization. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-17252-y
chicago: Zhang, J, E Mazur, J Balla, Michelle C Gallei, P Kalousek, Z Medveďová,
Y Li, et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin Transport
Canalization.” Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-17252-y.
ieee: J. Zhang et al., “Strigolactones inhibit auxin feedback on PIN-dependent
auxin transport canalization,” Nature Communications, vol. 11, no. 1. Springer
Nature, p. 3508, 2020.
ista: Zhang J, Mazur E, Balla J, Gallei MC, Kalousek P, Medveďová Z, Li Y, Wang
Y, Prat T, Vasileva MK, Reinöhl V, Procházka S, Halouzka R, Tarkowski P, Luschnig
C, Brewer P, Friml J. 2020. Strigolactones inhibit auxin feedback on PIN-dependent
auxin transport canalization. Nature Communications. 11(1), 3508.
mla: Zhang, J., et al. “Strigolactones Inhibit Auxin Feedback on PIN-Dependent Auxin
Transport Canalization.” Nature Communications, vol. 11, no. 1, Springer
Nature, 2020, p. 3508, doi:10.1038/s41467-020-17252-y.
short: J. Zhang, E. Mazur, J. Balla, M.C. Gallei, P. Kalousek, Z. Medveďová, Y.
Li, Y. Wang, T. Prat, M.K. Vasileva, V. Reinöhl, S. Procházka, R. Halouzka, P.
Tarkowski, C. Luschnig, P. Brewer, J. Friml, Nature Communications 11 (2020) 3508.
date_created: 2020-07-21T08:58:07Z
date_published: 2020-07-14T00:00:00Z
date_updated: 2023-08-22T08:13:44Z
day: '14'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-020-17252-y
ec_funded: 1
external_id:
isi:
- '000550062200004'
pmid:
- '32665554'
file:
- access_level: open_access
content_type: application/pdf
creator: dernst
date_created: 2020-07-22T08:32:55Z
date_updated: 2020-07-22T08:32:55Z
file_id: '8148'
file_name: 2020_NatureComm_Zhang.pdf
file_size: 1759490
relation: main_file
success: 1
file_date_updated: 2020-07-22T08:32:55Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: '3508'
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Strigolactones inhibit auxin feedback on PIN-dependent auxin transport canalization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '8271'
acknowledgement: We thank Dr. Gai Huang for his comments and help. We apologize to
authors whose work could not be cited due to space limitation. No conflict of interest
declared.
article_processing_charge: No
article_type: original
author:
- first_name: Peng
full_name: He, Peng
last_name: He
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Guanghui
full_name: Xiao, Guanghui
last_name: Xiao
citation:
ama: He P, Zhang Y, Xiao G. Origin of a subgenome and genome evolution of allotetraploid
cotton species. Molecular Plant. 2020;13(9):1238-1240. doi:10.1016/j.molp.2020.07.006
apa: He, P., Zhang, Y., & Xiao, G. (2020). Origin of a subgenome and genome
evolution of allotetraploid cotton species. Molecular Plant. Elsevier.
https://doi.org/10.1016/j.molp.2020.07.006
chicago: He, Peng, Yuzhou Zhang, and Guanghui Xiao. “Origin of a Subgenome and Genome
Evolution of Allotetraploid Cotton Species.” Molecular Plant. Elsevier,
2020. https://doi.org/10.1016/j.molp.2020.07.006.
ieee: P. He, Y. Zhang, and G. Xiao, “Origin of a subgenome and genome evolution
of allotetraploid cotton species,” Molecular Plant, vol. 13, no. 9. Elsevier,
pp. 1238–1240, 2020.
ista: He P, Zhang Y, Xiao G. 2020. Origin of a subgenome and genome evolution of
allotetraploid cotton species. Molecular Plant. 13(9), 1238–1240.
mla: He, Peng, et al. “Origin of a Subgenome and Genome Evolution of Allotetraploid
Cotton Species.” Molecular Plant, vol. 13, no. 9, Elsevier, 2020, pp. 1238–40,
doi:10.1016/j.molp.2020.07.006.
short: P. He, Y. Zhang, G. Xiao, Molecular Plant 13 (2020) 1238–1240.
date_created: 2020-08-16T22:00:57Z
date_published: 2020-09-07T00:00:00Z
date_updated: 2023-08-22T08:40:35Z
day: '07'
department:
- _id: JiFr
doi: 10.1016/j.molp.2020.07.006
external_id:
isi:
- '000566895400007'
pmid:
- '32688032'
intvolume: ' 13'
isi: 1
issue: '9'
language:
- iso: eng
month: '09'
oa_version: None
page: 1238-1240
pmid: 1
publication: Molecular Plant
publication_identifier:
eissn:
- '17529867'
issn:
- '16742052'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Origin of a subgenome and genome evolution of allotetraploid cotton species
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2020'
...
---
_id: '8337'
abstract:
- lang: eng
text: Cytokinins are mobile multifunctional plant hormones with roles in development
and stress resilience. Although their Histidine Kinase receptors are substantially
localised to the endoplasmic reticulum, cellular sites of cytokinin perception
and importance of spatially heterogeneous cytokinin distribution continue to be
debated. Here we show that cytokinin perception by plasma membrane receptors is
an effective additional path for cytokinin response. Readout from a Two Component
Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular
cytokinin content in roots, yet we also find cytokinins in extracellular fluid,
potentially enabling action at the cell surface. Cytokinins covalently linked
to beads that could not pass the plasma membrane increased expression of both
TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled
receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin
receptor mutants, further indicate that receptors can function at the cell surface.
We argue that dual intracellular and surface locations may augment flexibility
of cytokinin responses.
acknowledged_ssus:
- _id: Bio
acknowledgement: 'We thank Bruno Müller and Aaron Rashotte for critical discussions
and provision of plant lines used in this work, Roger Granbom and Tamara Hernández
Verdeja (UPSC, Umeå, Sweden) for technical assistance and providing materials, Zuzana
Pěkná and Karolina Wojewodová (CRH, Palacký University, Olomouc, Czech Republic)
for help with cytokinin receptor binding assays, and David Zalabák (CRH, Palacký
University, Olomouc, Czech Republic) for provision of vector pINIIIΔEH expressing
CRE1/AHK4. The bioimaging facility of IST Austria, the Swedish Metabolomics Centre
and the IST Austria Bio-Imaging facility are acknowledged for support. The work
was funded by the European Molecular Biology Organization (EMBO ASTF 297-2013) (I.A.),
Development—The Company of Biologists (DEVTF2012) (I.A.; C.T.), Plant Fellows (the
International Post doc Fellowship Programme in Plant Sciences, 267423) (I.A.; K.L.),
the Swedish Research Council (621-2014-4514) (K.L.), UPSC Berzelii Center for Forest
Biotechnology (Vinnova 2012-01560), Kempestiftelserna (JCK-2711) (K.L.) and (JCK-1811)
(E.-M.B., K.L.). The Ministry of Education, Youth and Sports of the Czech Republic
via the European Regional Development Fund-Project “Plants as a tool for sustainable
global development” (CZ.02.1.01/0.0/0.0/16_019/0000827) (O.N., O.P., R.S., V.M.,
L.P., K.D.) and project CEITEC 2020 (LQ1601) (M.P., J.H.) provided support, as did
the Czech Science Foundation via projects GP14-30004P (M.P.) and 16-04184S (O.P.,
K.D., O.N.), Vetenskapsrådet and Vinnova (Verket för Innovationssystem) (T.V., S.R.),
Knut och Alice Wallenbergs Stiftelse via “Shapesystem” grant number 2012.0050. A.J.
was supported by the Austria Science Fund (FWF): I03630 to J.F. The research leading
to these results received funding from European Union’s Horizon 2020 programme (ERC
grant no. 742985) and FWO-FWF joint project G0E5718N to J.F.'
article_number: '4284'
article_processing_charge: No
article_type: original
author:
- first_name: Ioanna
full_name: Antoniadi, Ioanna
last_name: Antoniadi
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Ondřej
full_name: Plíhal, Ondřej
last_name: Plíhal
- first_name: Radim
full_name: Simerský, Radim
last_name: Simerský
- first_name: Václav
full_name: Mik, Václav
last_name: Mik
- first_name: Thomas
full_name: Vain, Thomas
last_name: Vain
- first_name: Eduardo
full_name: Mateo-Bonmatí, Eduardo
last_name: Mateo-Bonmatí
- first_name: Michal
full_name: Karady, Michal
last_name: Karady
- first_name: Markéta
full_name: Pernisová, Markéta
last_name: Pernisová
- first_name: Lenka
full_name: Plačková, Lenka
last_name: Plačková
- first_name: Korawit
full_name: Opassathian, Korawit
last_name: Opassathian
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Karel
full_name: Doležal, Karel
last_name: Doležal
- first_name: Karin
full_name: Ljung, Karin
last_name: Ljung
- first_name: Colin
full_name: Turnbull, Colin
last_name: Turnbull
citation:
ama: Antoniadi I, Novák O, Gelová Z, et al. Cell-surface receptors enable perception
of extracellular cytokinins. Nature Communications. 2020;11. doi:10.1038/s41467-020-17700-9
apa: Antoniadi, I., Novák, O., Gelová, Z., Johnson, A. J., Plíhal, O., Simerský,
R., … Turnbull, C. (2020). Cell-surface receptors enable perception of extracellular
cytokinins. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-17700-9
chicago: Antoniadi, Ioanna, Ondřej Novák, Zuzana Gelová, Alexander J Johnson, Ondřej
Plíhal, Radim Simerský, Václav Mik, et al. “Cell-Surface Receptors Enable Perception
of Extracellular Cytokinins.” Nature Communications. Springer Nature, 2020.
https://doi.org/10.1038/s41467-020-17700-9.
ieee: I. Antoniadi et al., “Cell-surface receptors enable perception of extracellular
cytokinins,” Nature Communications, vol. 11. Springer Nature, 2020.
ista: Antoniadi I, Novák O, Gelová Z, Johnson AJ, Plíhal O, Simerský R, Mik V, Vain
T, Mateo-Bonmatí E, Karady M, Pernisová M, Plačková L, Opassathian K, Hejátko
J, Robert S, Friml J, Doležal K, Ljung K, Turnbull C. 2020. Cell-surface receptors
enable perception of extracellular cytokinins. Nature Communications. 11, 4284.
mla: Antoniadi, Ioanna, et al. “Cell-Surface Receptors Enable Perception of Extracellular
Cytokinins.” Nature Communications, vol. 11, 4284, Springer Nature, 2020,
doi:10.1038/s41467-020-17700-9.
short: I. Antoniadi, O. Novák, Z. Gelová, A.J. Johnson, O. Plíhal, R. Simerský,
V. Mik, T. Vain, E. Mateo-Bonmatí, M. Karady, M. Pernisová, L. Plačková, K. Opassathian,
J. Hejátko, S. Robert, J. Friml, K. Doležal, K. Ljung, C. Turnbull, Nature Communications
11 (2020).
date_created: 2020-09-06T22:01:13Z
date_published: 2020-08-27T00:00:00Z
date_updated: 2023-08-22T09:10:32Z
day: '27'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-020-17700-9
ec_funded: 1
external_id:
isi:
- '000567931000001'
file:
- access_level: open_access
checksum: 5b96f39b598de7510cfefefb819b9a6d
content_type: application/pdf
creator: dernst
date_created: 2020-12-10T12:23:56Z
date_updated: 2020-12-10T12:23:56Z
file_id: '8936'
file_name: 2020_NatureComm_Antoniadi.pdf
file_size: 3526415
relation: main_file
success: 1
file_date_updated: 2020-12-10T12:23:56Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Communications
publication_identifier:
eissn:
- '20411723'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell-surface receptors enable perception of extracellular cytokinins
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '8721'
abstract:
- lang: eng
text: Spontaneously arising channels that transport the phytohormone auxin provide
positional cues for self-organizing aspects of plant development such as flexible
vasculature regeneration or its patterning during leaf venation. The auxin canalization
hypothesis proposes a feedback between auxin signaling and transport as the underlying
mechanism, but molecular players await discovery. We identified part of the machinery
that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related
Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like
kinase) interact with and phosphorylate PIN auxin transporters. camel and canar
mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization,
which macroscopically manifests as defects in leaf venation and vasculature regeneration
after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback
that coordinates polarization of individual cells during auxin canalization.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: 'We acknowledge M. Glanc and Y. Zhang for providing entryclones;
Vienna Biocenter Core Facilities (VBCF) for recombinantprotein production and purification;
Vienna Biocenter Massspectrometry Facility, Bioimaging, and Life Science Facilities
at IST Austria and Proteomics Core Facility CEITEC for a great assistance.Funding:This
project received funding from the European Research Council (ERC) under the European
Union’s Horizon 2020 research and innovation program (grant agreement 742985) and
Austrian Science Fund (FWF): I 3630-B25 to J.F.and by grants from the Austrian Academy
of Science through the Gregor Mendel Institute (Y.B.) and the Austrian Agency for
International Cooperation in Education and Research (D.D.); the Netherlands Organization
for Scientific Research (NWO; VIDI-864.13.001) (W.S.); the Research Foundation–Flanders
(FWO;Odysseus II G0D0515N) and a European Research Council grant (ERC; StG TORPEDO;
714055) to B.D.R., B.Y., and E.M.; and the Hertha Firnberg Programme postdoctoral
fellowship (T-947) from the FWF Austrian Science Fund to E.S.-L.; J.H. is the recipient
of a DOC Fellowship of the Austrian Academy of Sciences at IST Austria.'
article_processing_charge: No
article_type: original
author:
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Tomas
full_name: Prat, Tomas
id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
last_name: Prat
- first_name: N
full_name: Rydza, N
last_name: Rydza
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: David
full_name: Domjan, David
id: C684CD7A-257E-11EA-9B6F-D8588B4F947F
last_name: Domjan
orcid: 0000-0003-2267-106X
- first_name: E
full_name: Mazur, E
last_name: Mazur
- first_name: E
full_name: Smakowska-Luzan, E
last_name: Smakowska-Luzan
- first_name: W
full_name: Smet, W
last_name: Smet
- first_name: E
full_name: Mor, E
last_name: Mor
- first_name: J
full_name: Nolf, J
last_name: Nolf
- first_name: B
full_name: Yang, B
last_name: Yang
- first_name: W
full_name: Grunewald, W
last_name: Grunewald
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Y
full_name: Belkhadir, Y
last_name: Belkhadir
- first_name: B
full_name: De Rybel, B
last_name: De Rybel
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hajny J, Prat T, Rydza N, et al. Receptor kinase module targets PIN-dependent
auxin transport during canalization. Science. 2020;370(6516):550-557. doi:10.1126/science.aba3178
apa: Hajny, J., Prat, T., Rydza, N., Rodriguez Solovey, L., Tan, S., Verstraeten,
I., … Friml, J. (2020). Receptor kinase module targets PIN-dependent auxin transport
during canalization. Science. American Association for the Advancement
of Science. https://doi.org/10.1126/science.aba3178
chicago: Hajny, Jakub, Tomas Prat, N Rydza, Lesia Rodriguez Solovey, Shutang Tan,
Inge Verstraeten, David Domjan, et al. “Receptor Kinase Module Targets PIN-Dependent
Auxin Transport during Canalization.” Science. American Association for
the Advancement of Science, 2020. https://doi.org/10.1126/science.aba3178.
ieee: J. Hajny et al., “Receptor kinase module targets PIN-dependent auxin
transport during canalization,” Science, vol. 370, no. 6516. American Association
for the Advancement of Science, pp. 550–557, 2020.
ista: Hajny J, Prat T, Rydza N, Rodriguez Solovey L, Tan S, Verstraeten I, Domjan
D, Mazur E, Smakowska-Luzan E, Smet W, Mor E, Nolf J, Yang B, Grunewald W, Molnar
G, Belkhadir Y, De Rybel B, Friml J. 2020. Receptor kinase module targets PIN-dependent
auxin transport during canalization. Science. 370(6516), 550–557.
mla: Hajny, Jakub, et al. “Receptor Kinase Module Targets PIN-Dependent Auxin Transport
during Canalization.” Science, vol. 370, no. 6516, American Association
for the Advancement of Science, 2020, pp. 550–57, doi:10.1126/science.aba3178.
short: J. Hajny, T. Prat, N. Rydza, L. Rodriguez Solovey, S. Tan, I. Verstraeten,
D. Domjan, E. Mazur, E. Smakowska-Luzan, W. Smet, E. Mor, J. Nolf, B. Yang, W.
Grunewald, G. Molnar, Y. Belkhadir, B. De Rybel, J. Friml, Science 370 (2020)
550–557.
date_created: 2020-11-02T10:04:46Z
date_published: 2020-10-30T00:00:00Z
date_updated: 2023-09-05T12:02:35Z
day: '30'
department:
- _id: JiFr
doi: 10.1126/science.aba3178
ec_funded: 1
external_id:
isi:
- '000583031800041'
pmid:
- '33122378'
intvolume: ' 370'
isi: 1
issue: '6516'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://europepmc.org/article/MED/33122378#free-full-text
month: '10'
oa: 1
oa_version: Published Version
page: 550-557
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2699E3D2-B435-11E9-9278-68D0E5697425
grant_number: '25239'
name: Cell surface receptor complexes for PIN polarity and auxin-mediated development
publication: Science
publication_identifier:
eissn:
- 1095-9203
issn:
- 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/molecular-compass-for-cell-orientation/
scopus_import: '1'
status: public
title: Receptor kinase module targets PIN-dependent auxin transport during canalization
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 370
year: '2020'
...
---
_id: '7949'
abstract:
- lang: eng
text: Peptides derived from non-functional precursors play important roles in various
developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide
analyses of C-terminally encoded peptide 5 (CEP5)-mediated changes revealed an
impact on abiotic stress-related processes. Drought has a dramatic impact on plant
growth, development and reproduction, and the plant hormone auxin plays a role
in drought responses. Our genetic, physiological, biochemical and pharmacological
results demonstrated that CEP5-mediated signaling is relevant for osmotic and
drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts
auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional
repressors, suggesting the existence of a novel peptide-dependent control mechanism
that tunes auxin signaling. These observations align with the recently described
role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic
and drought stress tolerance.
acknowledgement: We thank Maria Njo, Sarah De Cokere, Marieke Mispelaere and Darren
Wells, for practical assistance, Daniël Van Damme for assistance with image analysis,
Marnik Vuylsteke for advice on statistics, Catherine Perrot-Rechenmann for useful
discussions, Steffen Lau for critical reading oft he manuscript, and Philip Benfey,
Gerd Jürgens, Philippe Nacry, Frederik Börnke, and Frans Tax for sharing materials.
article_processing_charge: No
article_type: original
author:
- first_name: S
full_name: Smith, S
last_name: Smith
- first_name: S
full_name: Zhu, S
last_name: Zhu
- first_name: L
full_name: Joos, L
last_name: Joos
- first_name: I
full_name: Roberts, I
last_name: Roberts
- first_name: N
full_name: Nikonorova, N
last_name: Nikonorova
- first_name: LD
full_name: Vu, LD
last_name: Vu
- first_name: E
full_name: Stes, E
last_name: Stes
- first_name: H
full_name: Cho, H
last_name: Cho
- first_name: A
full_name: Larrieu, A
last_name: Larrieu
- first_name: W
full_name: Xuan, W
last_name: Xuan
- first_name: B
full_name: Goodall, B
last_name: Goodall
- first_name: B
full_name: van de Cotte, B
last_name: van de Cotte
- first_name: JM
full_name: Waite, JM
last_name: Waite
- first_name: A
full_name: Rigal, A
last_name: Rigal
- first_name: SR
full_name: R Harborough, SR
last_name: R Harborough
- first_name: G
full_name: Persiau, G
last_name: Persiau
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
- first_name: GK
full_name: Kirschner, GK
last_name: Kirschner
- first_name: E
full_name: Vandermarliere, E
last_name: Vandermarliere
- first_name: L
full_name: Martens, L
last_name: Martens
- first_name: Y
full_name: Stahl, Y
last_name: Stahl
- first_name: D
full_name: Audenaert, D
last_name: Audenaert
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Felix, G
last_name: Felix
- first_name: R
full_name: Simon, R
last_name: Simon
- first_name: M
full_name: Bennett, M
last_name: Bennett
- first_name: A
full_name: Bishopp, A
last_name: Bishopp
- first_name: G
full_name: De Jaeger, G
last_name: De Jaeger
- first_name: K
full_name: Ljung, K
last_name: Ljung
- first_name: S
full_name: Kepinski, S
last_name: Kepinski
- first_name: S
full_name: Robert, S
last_name: Robert
- first_name: J
full_name: Nemhauser, J
last_name: Nemhauser
- first_name: I
full_name: Hwang, I
last_name: Hwang
- first_name: K
full_name: Gevaert, K
last_name: Gevaert
- first_name: T
full_name: Beeckman, T
last_name: Beeckman
- first_name: I
full_name: De Smet, I
last_name: De Smet
citation:
ama: Smith S, Zhu S, Joos L, et al. The CEP5 peptide promotes abiotic stress tolerance,
as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium
in Arabidopsis. Molecular & Cellular Proteomics. 2020;19(8):1248-1262.
doi:10.1074/mcp.ra119.001826
apa: Smith, S., Zhu, S., Joos, L., Roberts, I., Nikonorova, N., Vu, L., … De Smet,
I. (2020). The CEP5 peptide promotes abiotic stress tolerance, as revealed by
quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis.
Molecular & Cellular Proteomics. American Society for Biochemistry
and Molecular Biology. https://doi.org/10.1074/mcp.ra119.001826
chicago: Smith, S, S Zhu, L Joos, I Roberts, N Nikonorova, LD Vu, E Stes, et al.
“The CEP5 Peptide Promotes Abiotic Stress Tolerance, as Revealed by Quantitative
Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis.” Molecular
& Cellular Proteomics. American Society for Biochemistry and Molecular
Biology, 2020. https://doi.org/10.1074/mcp.ra119.001826.
ieee: S. Smith et al., “The CEP5 peptide promotes abiotic stress tolerance,
as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium
in Arabidopsis,” Molecular & Cellular Proteomics, vol. 19, no. 8. American
Society for Biochemistry and Molecular Biology, pp. 1248–1262, 2020.
ista: Smith S, Zhu S, Joos L, Roberts I, Nikonorova N, Vu L, Stes E, Cho H, Larrieu
A, Xuan W, Goodall B, van de Cotte B, Waite J, Rigal A, R Harborough S, Persiau
G, Vanneste S, Kirschner G, Vandermarliere E, Martens L, Stahl Y, Audenaert D,
Friml J, Felix G, Simon R, Bennett M, Bishopp A, De Jaeger G, Ljung K, Kepinski
S, Robert S, Nemhauser J, Hwang I, Gevaert K, Beeckman T, De Smet I. 2020. The
CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics,
and attenuates the AUX/IAA equilibrium in Arabidopsis. Molecular & Cellular
Proteomics. 19(8), 1248–1262.
mla: Smith, S., et al. “The CEP5 Peptide Promotes Abiotic Stress Tolerance, as Revealed
by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis.”
Molecular & Cellular Proteomics, vol. 19, no. 8, American Society for
Biochemistry and Molecular Biology, 2020, pp. 1248–62, doi:10.1074/mcp.ra119.001826.
short: S. Smith, S. Zhu, L. Joos, I. Roberts, N. Nikonorova, L. Vu, E. Stes, H.
Cho, A. Larrieu, W. Xuan, B. Goodall, B. van de Cotte, J. Waite, A. Rigal, S.
R Harborough, G. Persiau, S. Vanneste, G. Kirschner, E. Vandermarliere, L. Martens,
Y. Stahl, D. Audenaert, J. Friml, G. Felix, R. Simon, M. Bennett, A. Bishopp,
G. De Jaeger, K. Ljung, S. Kepinski, S. Robert, J. Nemhauser, I. Hwang, K. Gevaert,
T. Beeckman, I. De Smet, Molecular & Cellular Proteomics 19 (2020) 1248–1262.
date_created: 2020-06-08T10:10:53Z
date_published: 2020-08-01T00:00:00Z
date_updated: 2023-09-05T12:17:46Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1074/mcp.ra119.001826
external_id:
isi:
- '000561114000001'
pmid:
- '32404488'
file:
- access_level: open_access
checksum: 3f3f37b4a1ba2cfd270fc7733dd89680
content_type: application/pdf
creator: kschuh
date_created: 2021-05-05T10:10:14Z
date_updated: 2021-05-05T10:10:14Z
file_id: '9373'
file_name: 2020_MCP_Smith.pdf
file_size: 1632311
relation: main_file
success: 1
file_date_updated: 2021-05-05T10:10:14Z
has_accepted_license: '1'
intvolume: ' 19'
isi: 1
issue: '8'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 1248-1262
pmid: 1
publication: Molecular & Cellular Proteomics
publication_identifier:
eissn:
- 1535-9484
publication_status: published
publisher: American Society for Biochemistry and Molecular Biology
quality_controlled: '1'
scopus_import: '1'
status: public
title: The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative
proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 19
year: '2020'
...
---
_id: '7619'
abstract:
- lang: eng
text: Cell polarity is a fundamental feature of all multicellular organisms. In
plants, prominent cell polarity markers are PIN auxin transporters crucial for
plant development. To identify novel components involved in cell polarity establishment
and maintenance, we carried out a forward genetic screening with PIN2:PIN1-HA;pin2
Arabidopsis plants, which ectopically express predominantly basally localized
PIN1 in the root epidermal cells leading to agravitropic root growth. From the
screen, we identified the regulator of PIN polarity 12 (repp12) mutation, which
restored gravitropic root growth and caused PIN1-HA polarity switch from basal
to apical side of root epidermal cells. Complementation experiments established
the repp12 causative mutation as an amino acid substitution in Aminophospholipid
ATPase3 (ALA3), a phospholipid flippase with predicted function in vesicle formation.
ala3 T-DNA mutants show defects in many auxin-regulated processes, in asymmetric
auxin distribution and in PIN trafficking. Analysis of quintuple and sextuple
mutants confirmed a crucial role of ALA proteins in regulating plant development
and in PIN trafficking and polarity. Genetic and physical interaction studies
revealed that ALA3 functions together with GNOM and BIG3 ARF GEFs. Taken together,
our results identified ALA3 flippase as an important interactor and regulator
of ARF GEF functioning in PIN polarity, trafficking and auxin-mediated development.
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
article_type: original
author:
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Vendula
full_name: Pukyšová, Vendula
last_name: Pukyšová
- first_name: Adrià Sans
full_name: Sánchez, Adrià Sans
last_name: Sánchez
- first_name: Vivek Kumar
full_name: Raxwal, Vivek Kumar
last_name: Raxwal
- first_name: Christian S.
full_name: Hardtke, Christian S.
last_name: Hardtke
- first_name: Tomasz
full_name: Nodzynski, Tomasz
last_name: Nodzynski
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang X, Adamowski M, Marhavá P, et al. Arabidopsis flippases cooperate with
ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin
transporters. The Plant Cell. 2020;32(5):1644-1664. doi:10.1105/tpc.19.00869
apa: Zhang, X., Adamowski, M., Marhavá, P., Tan, S., Zhang, Y., Rodriguez Solovey,
L., … Friml, J. (2020). Arabidopsis flippases cooperate with ARF GTPase exchange
factors to regulate the trafficking and polarity of PIN auxin transporters. The
Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.19.00869
chicago: Zhang, Xixi, Maciek Adamowski, Petra Marhavá, Shutang Tan, Yuzhou Zhang,
Lesia Rodriguez Solovey, Marta Zwiewka, et al. “Arabidopsis Flippases Cooperate
with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN
Auxin Transporters.” The Plant Cell. American Society of Plant Biologists,
2020. https://doi.org/10.1105/tpc.19.00869.
ieee: X. Zhang et al., “Arabidopsis flippases cooperate with ARF GTPase exchange
factors to regulate the trafficking and polarity of PIN auxin transporters,” The
Plant Cell, vol. 32, no. 5. American Society of Plant Biologists, pp. 1644–1664,
2020.
ista: Zhang X, Adamowski M, Marhavá P, Tan S, Zhang Y, Rodriguez Solovey L, Zwiewka
M, Pukyšová V, Sánchez AS, Raxwal VK, Hardtke CS, Nodzynski T, Friml J. 2020.
Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the
trafficking and polarity of PIN auxin transporters. The Plant Cell. 32(5), 1644–1664.
mla: Zhang, Xixi, et al. “Arabidopsis Flippases Cooperate with ARF GTPase Exchange
Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.” The
Plant Cell, vol. 32, no. 5, American Society of Plant Biologists, 2020, pp.
1644–64, doi:10.1105/tpc.19.00869.
short: X. Zhang, M. Adamowski, P. Marhavá, S. Tan, Y. Zhang, L. Rodriguez Solovey,
M. Zwiewka, V. Pukyšová, A.S. Sánchez, V.K. Raxwal, C.S. Hardtke, T. Nodzynski,
J. Friml, The Plant Cell 32 (2020) 1644–1664.
date_created: 2020-03-28T07:39:22Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2023-09-05T12:21:06Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.19.00869
ec_funded: 1
external_id:
isi:
- '000545741500030'
pmid:
- '32193204'
intvolume: ' 32'
isi: 1
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1105/tpc.19.00869
month: '05'
oa: 1
oa_version: Published Version
page: 1644-1664
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: The Plant Cell
publication_identifier:
eissn:
- 1532-298X
issn:
- 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate
the trafficking and polarity of PIN auxin transporters
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 32
year: '2020'
...
---
_id: '8607'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily
conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein
complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles through the
recognition of motifs based on tyrosine or di-leucine in their cytoplasmic tails.
However, in plants, very little is known on how PM proteins are sorted for CME
and whether similar motifs are required. In Arabidopsis thaliana, the brassinosteroid
(BR) receptor, BR INSENSITIVE1 (BRI1), undergoes endocytosis that depends on clathrin
and AP-2. Here we demonstrate that BRI1 binds directly to the medium AP-2 subunit,
AP2M. The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed
tyrosine-based endocytic motifs. The tyrosine-to-phenylalanine substitution in
Y898KAI reduced BRI1 internalization without affecting its kinase activity. Consistently,
plants carrying the BRI1Y898F mutation were hypersensitive to BRs. Our study demonstrates
that AP-2-dependent internalization of PM proteins via the recognition of functional
tyrosine motifs also operates in plants.
article_processing_charge: No
article_type: original
author:
- first_name: D
full_name: Liu, D
last_name: Liu
- first_name: R
full_name: Kumar, R
last_name: Kumar
- first_name: Claus
full_name: LAN, Claus
last_name: LAN
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: W
full_name: Siao, W
last_name: Siao
- first_name: I
full_name: Vanhoutte, I
last_name: Vanhoutte
- first_name: P
full_name: Wang, P
last_name: Wang
- first_name: KW
full_name: Bender, KW
last_name: Bender
- first_name: K
full_name: Yperman, K
last_name: Yperman
- first_name: S
full_name: Martins, S
last_name: Martins
- first_name: X
full_name: Zhao, X
last_name: Zhao
- first_name: G
full_name: Vert, G
last_name: Vert
- first_name: D
full_name: Van Damme, D
last_name: Van Damme
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: E
full_name: Russinova, E
last_name: Russinova
citation:
ama: Liu D, Kumar R, LAN C, et al. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is
partly driven by a canonical tyrosine-based Motif. Plant Cell. 2020;32(11):3598-3612.
doi:10.1105/tpc.20.00384
apa: Liu, D., Kumar, R., LAN, C., Johnson, A. J., Siao, W., Vanhoutte, I., … Russinova,
E. (2020). Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical
tyrosine-based Motif. Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.20.00384
chicago: Liu, D, R Kumar, Claus LAN, Alexander J Johnson, W Siao, I Vanhoutte, P
Wang, et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by
a Canonical Tyrosine-Based Motif.” Plant Cell. American Society of Plant
Biologists, 2020. https://doi.org/10.1105/tpc.20.00384.
ieee: D. Liu et al., “Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly
driven by a canonical tyrosine-based Motif,” Plant Cell, vol. 32, no. 11.
American Society of Plant Biologists, pp. 3598–3612, 2020.
ista: Liu D, Kumar R, LAN C, Johnson AJ, Siao W, Vanhoutte I, Wang P, Bender K,
Yperman K, Martins S, Zhao X, Vert G, Van Damme D, Friml J, Russinova E. 2020.
Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based
Motif. Plant Cell. 32(11), 3598–3612.
mla: Liu, D., et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven
by a Canonical Tyrosine-Based Motif.” Plant Cell, vol. 32, no. 11, American
Society of Plant Biologists, 2020, pp. 3598–612, doi:10.1105/tpc.20.00384.
short: D. Liu, R. Kumar, C. LAN, A.J. Johnson, W. Siao, I. Vanhoutte, P. Wang, K.
Bender, K. Yperman, S. Martins, X. Zhao, G. Vert, D. Van Damme, J. Friml, E. Russinova,
Plant Cell 32 (2020) 3598–3612.
date_created: 2020-10-05T12:45:16Z
date_published: 2020-11-01T00:00:00Z
date_updated: 2023-09-05T12:21:32Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.20.00384
ec_funded: 1
external_id:
isi:
- '000600226800021'
pmid:
- '32958564'
intvolume: ' 32'
isi: 1
issue: '11'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://europepmc.org/article/MED/32958564
month: '11'
oa: 1
oa_version: Published Version
page: 3598-3612
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Plant Cell
publication_identifier:
eissn:
- 1532-298x
issn:
- 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical
tyrosine-based Motif
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 32
year: '2020'
...
---
_id: '7695'
abstract:
- lang: eng
text: The TPLATE complex (TPC) is a key endocytic adaptor protein complex in plants.
TPC in Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved
subunits and two plant-specific subunits, AtEH1/Pan1 and AtEH2/Pan1, although
cytoplasmic proteins are not associated with the hexameric subcomplex in the cytoplasm.
To investigate the dynamic assembly of the octameric TPC at the plasma membrane
(PM), we performed state-of-the-art dual-color live cell imaging at physiological
and lowered temperatures. Lowering the temperature slowed down endocytosis, thereby
enhancing the temporal resolution of the differential recruitment of endocytic
components. Under both normal and lowered temperature conditions, the core TPC
subunit TPLATE and the AtEH/Pan1 proteins exhibited simultaneous recruitment at
the PM. These results, together with co-localization analysis of different TPC
subunits, allow us to conclude that TPC in plant cells is not recruited to the
PM sequentially but as an octameric complex.
article_processing_charge: No
article_type: original
author:
- first_name: J
full_name: Wang, J
last_name: Wang
- first_name: E
full_name: Mylle, E
last_name: Mylle
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: N
full_name: Besbrugge, N
last_name: Besbrugge
- first_name: G
full_name: De Jaeger, G
last_name: De Jaeger
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: R
full_name: Pleskot, R
last_name: Pleskot
- first_name: D
full_name: van Damme, D
last_name: van Damme
citation:
ama: Wang J, Mylle E, Johnson AJ, et al. High temporal resolution reveals simultaneous
plasma membrane recruitment of TPLATE complex subunits. Plant Physiology.
2020;183(3):986-997. doi:10.1104/pp.20.00178
apa: Wang, J., Mylle, E., Johnson, A. J., Besbrugge, N., De Jaeger, G., Friml, J.,
… van Damme, D. (2020). High temporal resolution reveals simultaneous plasma membrane
recruitment of TPLATE complex subunits. Plant Physiology. American Society
of Plant Biologists. https://doi.org/10.1104/pp.20.00178
chicago: Wang, J, E Mylle, Alexander J Johnson, N Besbrugge, G De Jaeger, Jiří Friml,
R Pleskot, and D van Damme. “High Temporal Resolution Reveals Simultaneous Plasma
Membrane Recruitment of TPLATE Complex Subunits.” Plant Physiology. American
Society of Plant Biologists, 2020. https://doi.org/10.1104/pp.20.00178.
ieee: J. Wang et al., “High temporal resolution reveals simultaneous plasma
membrane recruitment of TPLATE complex subunits,” Plant Physiology, vol.
183, no. 3. American Society of Plant Biologists, pp. 986–997, 2020.
ista: Wang J, Mylle E, Johnson AJ, Besbrugge N, De Jaeger G, Friml J, Pleskot R,
van Damme D. 2020. High temporal resolution reveals simultaneous plasma membrane
recruitment of TPLATE complex subunits. Plant Physiology. 183(3), 986–997.
mla: Wang, J., et al. “High Temporal Resolution Reveals Simultaneous Plasma Membrane
Recruitment of TPLATE Complex Subunits.” Plant Physiology, vol. 183, no.
3, American Society of Plant Biologists, 2020, pp. 986–97, doi:10.1104/pp.20.00178.
short: J. Wang, E. Mylle, A.J. Johnson, N. Besbrugge, G. De Jaeger, J. Friml, R.
Pleskot, D. van Damme, Plant Physiology 183 (2020) 986–997.
date_created: 2020-04-29T15:23:00Z
date_published: 2020-07-01T00:00:00Z
date_updated: 2023-09-05T12:20:02Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.20.00178
external_id:
isi:
- '000550682000018'
pmid:
- '32321842'
intvolume: ' 183'
isi: 1
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2020.02.13.948109
month: '07'
oa: 1
oa_version: Preprint
page: 986-997
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: High temporal resolution reveals simultaneous plasma membrane recruitment of
TPLATE complex subunits
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 183
year: '2020'
...
---
_id: '7697'
abstract:
- lang: eng
text: "* Morphogenesis and adaptive tropic growth in plants depend on gradients
of the phytohormone auxin, mediated by the membrane‐based PIN‐FORMED (PIN) auxin
transporters. PINs localize to a particular side of the plasma membrane (PM) or
to the endoplasmic reticulum (ER) to directionally transport auxin and maintain
intercellular and intracellular auxin homeostasis, respectively. However, the
molecular cues that confer their diverse cellular localizations remain largely
unknown.\r\n* In this study, we systematically swapped the domains between ER‐
and PM‐localized PIN proteins, as well as between apical and basal PM‐localized
PINs from Arabidopsis thaliana , to shed light on why PIN family members with
similar topological structures reside at different membrane compartments within
cells.\r\n* Our results show that not only do the N‐ and C‐terminal transmembrane
domains (TMDs) and central hydrophilic loop contribute to their differential subcellular
localizations and cellular polarity, but that the pairwise‐matched N‐ and C‐terminal
TMDs resulting from intramolecular domain–domain coevolution are also crucial
for their divergent patterns of localization.\r\n* These findings illustrate the
complexity of the evolutionary path of PIN proteins in acquiring their plethora
of developmental functions and adaptive growth in plants."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Corinna
full_name: Hartinger, Corinna
id: AEFB2266-8ABF-11EA-AA39-812C3623CBE4
last_name: Hartinger
orcid: 0000-0003-1618-2737
- first_name: Xiaojuan
full_name: Wang, Xiaojuan
last_name: Wang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Hartinger C, Wang X, Friml J. Directional auxin fluxes in plants by
intramolecular domain‐domain co‐evolution of PIN auxin transporters. New Phytologist.
2020;227(5):1406-1416. doi:10.1111/nph.16629
apa: Zhang, Y., Hartinger, C., Wang, X., & Friml, J. (2020). Directional auxin
fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters.
New Phytologist. Wiley. https://doi.org/10.1111/nph.16629
chicago: Zhang, Yuzhou, Corinna Hartinger, Xiaojuan Wang, and Jiří Friml. “Directional
Auxin Fluxes in Plants by Intramolecular Domain‐domain Co‐evolution of PIN Auxin
Transporters.” New Phytologist. Wiley, 2020. https://doi.org/10.1111/nph.16629.
ieee: Y. Zhang, C. Hartinger, X. Wang, and J. Friml, “Directional auxin fluxes in
plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters,”
New Phytologist, vol. 227, no. 5. Wiley, pp. 1406–1416, 2020.
ista: Zhang Y, Hartinger C, Wang X, Friml J. 2020. Directional auxin fluxes in plants
by intramolecular domain‐domain co‐evolution of PIN auxin transporters. New Phytologist.
227(5), 1406–1416.
mla: Zhang, Yuzhou, et al. “Directional Auxin Fluxes in Plants by Intramolecular
Domain‐domain Co‐evolution of PIN Auxin Transporters.” New Phytologist,
vol. 227, no. 5, Wiley, 2020, pp. 1406–16, doi:10.1111/nph.16629.
short: Y. Zhang, C. Hartinger, X. Wang, J. Friml, New Phytologist 227 (2020) 1406–1416.
date_created: 2020-04-30T08:43:29Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2023-09-05T15:46:04Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.16629
ec_funded: 1
external_id:
isi:
- '000534092400001'
pmid:
- '32350870'
file:
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checksum: 8e8150dbbba8cb65b72f81d1f0864b8b
content_type: application/pdf
creator: dernst
date_created: 2020-11-24T12:19:38Z
date_updated: 2020-11-24T12:19:38Z
file_id: '8799'
file_name: 2020_09_NewPhytologist_Zhang.pdf
file_size: 3643395
relation: main_file
success: 1
file_date_updated: 2020-11-24T12:19:38Z
has_accepted_license: '1'
intvolume: ' 227'
isi: 1
issue: '5'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: 1406-1416
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution
of PIN auxin transporters
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 227
year: '2020'
...
---
_id: '7417'
abstract:
- lang: eng
text: Previously, we reported that the allelic de-etiolated by zinc (dez) and trichome
birefringence (tbr) mutants exhibit photomorphogenic development in the dark,
which is enhanced by high Zn. TRICHOME BIREFRINGENCE-LIKE proteins had been implicated
in transferring acetyl groups to various hemicelluloses. Pectin O-acetylation
levels were lower in dark-grown dez seedlings than in the wild type. We observed
Zn-enhanced photomorphogenesis in the dark also in the reduced wall acetylation
2 (rwa2-3) mutant, which exhibits lowered O-acetylation levels of cell wall macromolecules
including pectins and xyloglucans, supporting a role for cell wall macromolecule
O-acetylation in the photomorphogenic phenotypes of rwa2-3 and dez. Application
of very short oligogalacturonides (vsOGs) restored skotomorphogenesis in dark-grown
dez and rwa2-3. Here we demonstrate that in dez, O-acetylation of non-pectin cell
wall components, notably of xyloglucan, is enhanced. Our results highlight the
complexity of cell wall homeostasis and indicate against an influence of xyloglucan
O-acetylation on light-dependent seedling development.
article_number: e1687185
article_processing_charge: No
article_type: original
author:
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: S.
full_name: Gille, S.
last_name: Gille
- first_name: M.
full_name: Pauly, M.
last_name: Pauly
- first_name: U.
full_name: Krämer, U.
last_name: Krämer
citation:
ama: Sinclair SA, Gille S, Pauly M, Krämer U. Regulation of acetylation of plant
cell wall components is complex and responds to external stimuli. Plant Signaling
& Behavior. 2020;15(1). doi:10.1080/15592324.2019.1687185
apa: Sinclair, S. A., Gille, S., Pauly, M., & Krämer, U. (2020). Regulation
of acetylation of plant cell wall components is complex and responds to external
stimuli. Plant Signaling & Behavior. Informa UK Limited. https://doi.org/10.1080/15592324.2019.1687185
chicago: Sinclair, Scott A, S. Gille, M. Pauly, and U. Krämer. “Regulation of Acetylation
of Plant Cell Wall Components Is Complex and Responds to External Stimuli.” Plant
Signaling & Behavior. Informa UK Limited, 2020. https://doi.org/10.1080/15592324.2019.1687185.
ieee: S. A. Sinclair, S. Gille, M. Pauly, and U. Krämer, “Regulation of acetylation
of plant cell wall components is complex and responds to external stimuli,” Plant
Signaling & Behavior, vol. 15, no. 1. Informa UK Limited, 2020.
ista: Sinclair SA, Gille S, Pauly M, Krämer U. 2020. Regulation of acetylation of
plant cell wall components is complex and responds to external stimuli. Plant
Signaling & Behavior. 15(1), e1687185.
mla: Sinclair, Scott A., et al. “Regulation of Acetylation of Plant Cell Wall Components
Is Complex and Responds to External Stimuli.” Plant Signaling & Behavior,
vol. 15, no. 1, e1687185, Informa UK Limited, 2020, doi:10.1080/15592324.2019.1687185.
short: S.A. Sinclair, S. Gille, M. Pauly, U. Krämer, Plant Signaling & Behavior
15 (2020).
date_created: 2020-01-30T10:14:14Z
date_published: 2020-01-01T00:00:00Z
date_updated: 2023-09-06T15:23:04Z
day: '01'
department:
- _id: JiFr
doi: 10.1080/15592324.2019.1687185
external_id:
isi:
- '000494907500001'
pmid:
- '31696770'
intvolume: ' 15'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7012154
month: '01'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Plant Signaling & Behavior
publication_identifier:
issn:
- 1559-2324
publication_status: published
publisher: Informa UK Limited
quality_controlled: '1'
scopus_import: '1'
status: public
title: Regulation of acetylation of plant cell wall components is complex and responds
to external stimuli
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 15
year: '2020'
...
---
_id: '8589'
abstract:
- lang: eng
text: The plant hormone auxin plays indispensable roles in plant growth and development.
An essential level of regulation in auxin action is the directional auxin transport
within cells. The establishment of auxin gradient in plant tissue has been attributed
to local auxin biosynthesis and directional intercellular auxin transport, which
both are controlled by various environmental and developmental signals. It is
well established that asymmetric auxin distribution in cells is achieved by polarly
localized PIN-FORMED (PIN) auxin efflux transporters. Despite the initial insights
into cellular mechanisms of PIN polarization obtained from the last decades, the
molecular mechanism and specific regulators mediating PIN polarization remains
elusive. In this thesis, we aim to find novel players in PIN subcellular polarity
regulation during Arabidopsis development. We first characterize the physiological
effect of piperonylic acid (PA) on Arabidopsis hypocotyl gravitropic bending and
PIN polarization. Secondly, we reveal the importance of SCFTIR1/AFB auxin signaling
pathway in shoot gravitropism bending termination. In addition, we also explore
the role of myosin XI complex, and actin cytoskeleton in auxin feedback regulation
on PIN polarity. In Chapter 1, we give an overview of the current knowledge about
PIN-mediated auxin fluxes in various plant tropic responses. In Chapter 2, we
study the physiological effect of PA on shoot gravitropic bending. Our results
show that PA treatment inhibits auxin-mediated PIN3 repolarization by interfering
with PINOID and PIN3 phosphorylation status, ultimately leading to hyperbending
hypocotyls. In Chapter 3, we provide evidence to show that the SCFTIR1/AFB nuclear
auxin signaling pathway is crucial and required for auxin-mediated PIN3 repolarization
and shoot gravitropic bending termination. In Chapter 4, we perform a phosphoproteomics
approach and identify the motor protein Myosin XI and its binding protein, the
MadB2 family, as an essential regulator of PIN polarity for auxin-canalization
related developmental processes. In Chapter 5, we demonstrate the vital role of
actin cytoskeleton in auxin feedback on PIN polarity by regulating PIN subcellular
trafficking. Overall, the data presented in this PhD thesis brings novel insights
into the PIN polar localization regulation that resulted in the (re)establishment
of the polar auxin flow and gradient in response to environmental stimuli during
plant development.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: I also want to thank the China Scholarship Council for supporting
my study during the year from 2015 to 2019. I also want to thank IST facilities
– the Bioimaging facility, the media kitchen, the plant facility and all of the
campus services, for their support.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
citation:
ama: Han H. Novel insights into PIN polarity regulation during Arabidopsis development.
2020. doi:10.15479/AT:ISTA:8589
apa: Han, H. (2020). Novel insights into PIN polarity regulation during Arabidopsis
development. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:8589
chicago: Han, Huibin. “Novel Insights into PIN Polarity Regulation during Arabidopsis
Development.” Institute of Science and Technology Austria, 2020. https://doi.org/10.15479/AT:ISTA:8589.
ieee: H. Han, “Novel insights into PIN polarity regulation during Arabidopsis development,”
Institute of Science and Technology Austria, 2020.
ista: Han H. 2020. Novel insights into PIN polarity regulation during Arabidopsis
development. Institute of Science and Technology Austria.
mla: Han, Huibin. Novel Insights into PIN Polarity Regulation during Arabidopsis
Development. Institute of Science and Technology Austria, 2020, doi:10.15479/AT:ISTA:8589.
short: H. Han, Novel Insights into PIN Polarity Regulation during Arabidopsis Development,
Institute of Science and Technology Austria, 2020.
date_created: 2020-09-30T14:50:51Z
date_published: 2020-09-30T00:00:00Z
date_updated: 2023-09-07T13:13:05Z
day: '30'
ddc:
- '580'
degree_awarded: PhD
department:
- _id: JiFr
doi: 10.15479/AT:ISTA:8589
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date_created: 2020-09-30T14:50:20Z
date_updated: 2020-09-30T14:50:20Z
file_id: '8590'
file_name: 2020_Han_Thesis.docx
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date_created: 2020-09-30T14:49:59Z
date_updated: 2021-10-01T13:33:02Z
file_id: '8591'
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file_date_updated: 2021-10-01T13:33:02Z
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language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: '164'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '7643'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Novel insights into PIN polarity regulation during Arabidopsis development
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2020'
...
---
_id: '7643'
acknowledgement: 'This work was supported by the European Research Council under the
European Union’s Horizon 2020 research and innovation Programme (ERC grant agreement
number 742985), and the Austrian Science Fund (FWF, grant number I 3630-B25) to
JF. HH is supported by the China Scholarship Council (CSC scholarship). '
article_processing_charge: No
article_type: letter_note
author:
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Hana
full_name: Rakusova, Hana
id: 4CAAA450-78D2-11EA-8E57-B40A396E08BA
last_name: Rakusova
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Han H, Rakusova H, Verstraeten I, Zhang Y, Friml J. SCF TIR1/AFB auxin signaling
for bending termination during shoot gravitropism. Plant Physiology. 2020;183(5):37-40.
doi:10.1104/pp.20.00212
apa: Han, H., Rakusova, H., Verstraeten, I., Zhang, Y., & Friml, J. (2020).
SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism.
Plant Physiology. American Society of Plant Biologists. https://doi.org/10.1104/pp.20.00212
chicago: Han, Huibin, Hana Rakusova, Inge Verstraeten, Yuzhou Zhang, and Jiří Friml.
“SCF TIR1/AFB Auxin Signaling for Bending Termination during Shoot Gravitropism.”
Plant Physiology. American Society of Plant Biologists, 2020. https://doi.org/10.1104/pp.20.00212.
ieee: H. Han, H. Rakusova, I. Verstraeten, Y. Zhang, and J. Friml, “SCF TIR1/AFB
auxin signaling for bending termination during shoot gravitropism,” Plant Physiology,
vol. 183, no. 5. American Society of Plant Biologists, pp. 37–40, 2020.
ista: Han H, Rakusova H, Verstraeten I, Zhang Y, Friml J. 2020. SCF TIR1/AFB auxin
signaling for bending termination during shoot gravitropism. Plant Physiology.
183(5), 37–40.
mla: Han, Huibin, et al. “SCF TIR1/AFB Auxin Signaling for Bending Termination during
Shoot Gravitropism.” Plant Physiology, vol. 183, no. 5, American Society
of Plant Biologists, 2020, pp. 37–40, doi:10.1104/pp.20.00212.
short: H. Han, H. Rakusova, I. Verstraeten, Y. Zhang, J. Friml, Plant Physiology
183 (2020) 37–40.
date_created: 2020-04-06T10:06:40Z
date_published: 2020-05-08T00:00:00Z
date_updated: 2023-09-07T13:13:04Z
day: '08'
department:
- _id: JiFr
doi: 10.1104/pp.20.00212
ec_funded: 1
external_id:
isi:
- '000536641800018'
pmid:
- '32107280'
intvolume: ' 183'
isi: 1
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1104/pp.20.00212
month: '05'
oa: 1
oa_version: Published Version
page: 37-40
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
related_material:
record:
- id: '8589'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: SCF TIR1/AFB auxin signaling for bending termination during shoot gravitropism
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 183
year: '2020'
...
---
_id: '7416'
abstract:
- lang: eng
text: Earlier, we demonstrated that transcript levels of METAL TOLERANCE PROTEIN2
(MTP2) and of HEAVY METAL ATPase2 (HMA2) increase strongly in roots of Arabidopsis
upon prolonged zinc (Zn) deficiency and respond to shoot physiological Zn status,
and not to the local Zn status in roots. This provided evidence for shoot-to-root
communication in the acclimation of plants to Zn deficiency. Zn-deficient soils
limit both the yield and quality of agricultural crops and can result in clinically
relevant nutritional Zn deficiency in human populations. Implementing Zn deficiency
during cultivation of the model plant Arabidopsis thaliana on agar-solidified
media is difficult because trace element contaminations are present in almost
all commercially available agars. Here, we demonstrate root morphological acclimations
to Zn deficiency on agar-solidified medium following the effective removal of
contaminants. These advancements allow reproducible phenotyping toward understanding
fundamental plant responses to deficiencies of Zn and other essential trace elements.
article_number: '1687175'
article_processing_charge: No
article_type: original
author:
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: U.
full_name: Krämer, U.
last_name: Krämer
citation:
ama: Sinclair SA, Krämer U. Generation of effective zinc-deficient agar-solidified
media allows identification of root morphology changes in response to zinc limitation.
Plant Signaling & Behavior. 2020;15(1). doi:10.1080/15592324.2019.1687175
apa: Sinclair, S. A., & Krämer, U. (2020). Generation of effective zinc-deficient
agar-solidified media allows identification of root morphology changes in response
to zinc limitation. Plant Signaling & Behavior. Taylor & Francis.
https://doi.org/10.1080/15592324.2019.1687175
chicago: Sinclair, Scott A, and U. Krämer. “Generation of Effective Zinc-Deficient
Agar-Solidified Media Allows Identification of Root Morphology Changes in Response
to Zinc Limitation.” Plant Signaling & Behavior. Taylor & Francis,
2020. https://doi.org/10.1080/15592324.2019.1687175.
ieee: S. A. Sinclair and U. Krämer, “Generation of effective zinc-deficient agar-solidified
media allows identification of root morphology changes in response to zinc limitation,”
Plant Signaling & Behavior, vol. 15, no. 1. Taylor & Francis, 2020.
ista: Sinclair SA, Krämer U. 2020. Generation of effective zinc-deficient agar-solidified
media allows identification of root morphology changes in response to zinc limitation.
Plant Signaling & Behavior. 15(1), 1687175.
mla: Sinclair, Scott A., and U. Krämer. “Generation of Effective Zinc-Deficient
Agar-Solidified Media Allows Identification of Root Morphology Changes in Response
to Zinc Limitation.” Plant Signaling & Behavior, vol. 15, no. 1, 1687175,
Taylor & Francis, 2020, doi:10.1080/15592324.2019.1687175.
short: S.A. Sinclair, U. Krämer, Plant Signaling & Behavior 15 (2020).
date_created: 2020-01-30T10:12:04Z
date_published: 2020-01-01T00:00:00Z
date_updated: 2023-10-17T09:01:48Z
day: '01'
department:
- _id: JiFr
doi: 10.1080/15592324.2019.1687175
external_id:
isi:
- '000494909300001'
pmid:
- '31696764'
intvolume: ' 15'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7012054
month: '01'
oa: 1
oa_version: Submitted Version
pmid: 1
publication: Plant Signaling & Behavior
publication_identifier:
issn:
- 1559-2324
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
scopus_import: '1'
status: public
title: Generation of effective zinc-deficient agar-solidified media allows identification
of root morphology changes in response to zinc limitation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2020'
...
---
_id: '8943'
abstract:
- lang: eng
text: The widely used non-steroidal anti-inflammatory drugs (NSAIDs) are derivatives
of the phytohormone salicylic acid (SA). SA is well known to regulate plant immunity
and development, whereas there have been few reports focusing on the effects of
NSAIDs in plants. Our studies here reveal that NSAIDs exhibit largely overlapping
physiological activities to SA in the model plant Arabidopsis. NSAID treatments
lead to shorter and agravitropic primary roots and inhibited lateral root organogenesis.
Notably, in addition to the SA-like action, which in roots involves binding to
the protein phosphatase 2A (PP2A), NSAIDs also exhibit PP2A-independent effects.
Cell biological and biochemical analyses reveal that many NSAIDs bind directly
to and inhibit the chaperone activity of TWISTED DWARF1, thereby regulating actin
cytoskeleton dynamics and subsequent endosomal trafficking. Our findings uncover
an unexpected bioactivity of human pharmaceuticals in plants and provide insights
into the molecular mechanism underlying the cellular action of this class of anti-inflammatory
compounds.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: "We thank Drs. Sebastian Bednarek (University of Wisconsin-Madison),
Niko Geldner (University of Lausanne), and Karin Schumacher (Heidelberg University)
for kindly sharing published Arabidopsis lines; Dr. Satoshi Naramoto for the pPIN2::PIN2-GFP;
pVHA-a1::VHA-a1-mRFP reporter; the staff at the Life Science Facility and Bioimaging
Facility, Monika Hrtyan, and Dorota Jaworska at IST Austria for technical support;
and Drs. Su Tang (Texas A&M University),\r\nMelinda Abas (BOKU), Eva Benkova´ (IST
Austria), Christian Luschnig (BOKU), Bartel Vanholme (Gent University), and the
Friml group for valuable discussions. The research leading to these findings was
funded by the European Union’s Horizon 2020 program (ERC grant agreement no. 742985,
to J.F.), the People Programme (Marie Curie Actions) of the European Union’s Seventh
Framework Programme (FP7/2007-2013) under REA grant agreement no.\r\n291734, the
Swiss National Funds (31003A_165877, to M.G.), the Ministry of Education, Youth,
and Sports of the Czech Republic (project no. CZ.02.1.01/0.0/0.0/16_019/0000738,
EU Operational Programme ‘‘Research, development and education and Centre for Plant
Experimental Biology’’), and the EU Operational Programme Prague - Competitiveness
(project no. CZ.2.16/3.1.00/21519). S.T. was funded by a European Molecular Biology
Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). X.Z. was
partly supported by a PhD scholarship from the China Scholarship Council."
article_number: '108463'
article_processing_charge: Yes
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Martin
full_name: Di Donato, Martin
last_name: Di Donato
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Petr
full_name: Klíma, Petr
last_name: Klíma
- first_name: Jie
full_name: Liu, Jie
last_name: Liu
- first_name: Aurélien
full_name: Bailly, Aurélien
last_name: Bailly
- first_name: Noel
full_name: Ferro, Noel
last_name: Ferro
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tan S, Di Donato M, Glanc M, et al. Non-steroidal anti-inflammatory drugs target
TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development.
Cell Reports. 2020;33(9). doi:10.1016/j.celrep.2020.108463
apa: Tan, S., Di Donato, M., Glanc, M., Zhang, X., Klíma, P., Liu, J., … Friml,
J. (2020). Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated
actin dynamics and auxin transport-mediated plant development. Cell Reports.
Elsevier. https://doi.org/10.1016/j.celrep.2020.108463
chicago: Tan, Shutang, Martin Di Donato, Matous Glanc, Xixi Zhang, Petr Klíma, Jie
Liu, Aurélien Bailly, et al. “Non-Steroidal Anti-Inflammatory Drugs Target TWISTED
DWARF1-Regulated Actin Dynamics and Auxin Transport-Mediated Plant Development.”
Cell Reports. Elsevier, 2020. https://doi.org/10.1016/j.celrep.2020.108463.
ieee: S. Tan et al., “Non-steroidal anti-inflammatory drugs target TWISTED
DWARF1-regulated actin dynamics and auxin transport-mediated plant development,”
Cell Reports, vol. 33, no. 9. Elsevier, 2020.
ista: Tan S, Di Donato M, Glanc M, Zhang X, Klíma P, Liu J, Bailly A, Ferro N, Petrášek
J, Geisler M, Friml J. 2020. Non-steroidal anti-inflammatory drugs target TWISTED
DWARF1-regulated actin dynamics and auxin transport-mediated plant development.
Cell Reports. 33(9), 108463.
mla: Tan, Shutang, et al. “Non-Steroidal Anti-Inflammatory Drugs Target TWISTED
DWARF1-Regulated Actin Dynamics and Auxin Transport-Mediated Plant Development.”
Cell Reports, vol. 33, no. 9, 108463, Elsevier, 2020, doi:10.1016/j.celrep.2020.108463.
short: S. Tan, M. Di Donato, M. Glanc, X. Zhang, P. Klíma, J. Liu, A. Bailly, N.
Ferro, J. Petrášek, M. Geisler, J. Friml, Cell Reports 33 (2020).
date_created: 2020-12-13T23:01:21Z
date_published: 2020-12-01T00:00:00Z
date_updated: 2023-11-16T13:03:31Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.celrep.2020.108463
ec_funded: 1
external_id:
isi:
- '000595658100018'
pmid:
- '33264621'
file:
- access_level: open_access
checksum: ed18cba0fb48ed2e789381a54cc21904
content_type: application/pdf
creator: dernst
date_created: 2020-12-14T07:33:39Z
date_updated: 2020-12-14T07:33:39Z
file_id: '8948'
file_name: 2020_CellReports_Tan.pdf
file_size: 8056434
relation: main_file
success: 1
file_date_updated: 2020-12-14T07:33:39Z
has_accepted_license: '1'
intvolume: ' 33'
isi: 1
issue: '9'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
grant_number: 723-2015
name: Long Term Fellowship
publication: Cell Reports
publication_identifier:
eissn:
- '22111247'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/plants-on-aspirin/
scopus_import: '1'
status: public
title: Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin
dynamics and auxin transport-mediated plant development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 33
year: '2020'
...
---
_id: '8002'
abstract:
- lang: eng
text: Wound healing in plant tissues, consisting of rigid cell wall-encapsulated
cells, represents a considerable challenge and occurs through largely unknown
mechanisms distinct from those in animals. Owing to their inability to migrate,
plant cells rely on targeted cell division and expansion to regenerate wounds.
Strict coordination of these wound-induced responses is essential to ensure efficient,
spatially restricted wound healing. Single-cell tracking by live imaging allowed
us to gain mechanistic insight into the wound perception and coordination of wound
responses after laser-based wounding in Arabidopsis root. We revealed a crucial
contribution of the collapse of damaged cells in wound perception and detected
an auxin increase specific to cells immediately adjacent to the wound. This localized
auxin increase balances wound-induced cell expansion and restorative division
rates in a dose-dependent manner, leading to tumorous overproliferation when the
canonical TIR1 auxin signaling is disrupted. Auxin and wound-induced turgor pressure
changes together also spatially define the activation of key components of regeneration,
such as the transcription regulator ERF115. Our observations suggest that the
wound signaling involves the sensing of collapse of damaged cells and a local
auxin signaling activation to coordinate the downstream transcriptional responses
in the immediate wound vicinity.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
article_number: '202003346'
article_processing_charge: No
article_type: original
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J.
Wounding-induced changes in cellular pressure and localized auxin signalling spatially
coordinate restorative divisions in roots. Proceedings of the National Academy
of Sciences. 2020;117(26). doi:10.1073/pnas.2003346117
apa: Hörmayer, L., Montesinos López, J. C., Marhavá, P., Benková, E., Yoshida, S.,
& Friml, J. (2020). Wounding-induced changes in cellular pressure and localized
auxin signalling spatially coordinate restorative divisions in roots. Proceedings
of the National Academy of Sciences. Proceedings of the National Academy of
Sciences. https://doi.org/10.1073/pnas.2003346117
chicago: Hörmayer, Lukas, Juan C Montesinos López, Petra Marhavá, Eva Benková, Saiko
Yoshida, and Jiří Friml. “Wounding-Induced Changes in Cellular Pressure and Localized
Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.” Proceedings
of the National Academy of Sciences. Proceedings of the National Academy of
Sciences, 2020. https://doi.org/10.1073/pnas.2003346117.
ieee: L. Hörmayer, J. C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, and
J. Friml, “Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots,” Proceedings of the National
Academy of Sciences, vol. 117, no. 26. Proceedings of the National Academy
of Sciences, 2020.
ista: Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J.
2020. Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots. Proceedings of the National
Academy of Sciences. 117(26), 202003346.
mla: Hörmayer, Lukas, et al. “Wounding-Induced Changes in Cellular Pressure and
Localized Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.”
Proceedings of the National Academy of Sciences, vol. 117, no. 26, 202003346,
Proceedings of the National Academy of Sciences, 2020, doi:10.1073/pnas.2003346117.
short: L. Hörmayer, J.C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, J.
Friml, Proceedings of the National Academy of Sciences 117 (2020).
date_created: 2020-06-22T13:33:52Z
date_published: 2020-06-30T00:00:00Z
date_updated: 2024-03-28T23:30:10Z
day: '30'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1073/pnas.2003346117
ec_funded: 1
external_id:
isi:
- '000565729700033'
pmid:
- '32541049'
file:
- access_level: open_access
checksum: 908b09437680181de9990915f2113aca
content_type: application/pdf
creator: dernst
date_created: 2020-06-23T11:30:53Z
date_updated: 2020-07-14T12:48:07Z
file_id: '8009'
file_name: 2020_PNAS_Hoermayer.pdf
file_size: 2407102
relation: main_file
file_date_updated: 2020-07-14T12:48:07Z
has_accepted_license: '1'
intvolume: ' 117'
isi: 1
issue: '26'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '06'
oa: 1
oa_version: None
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/how-wounded-plants-coordinate-their-healing/
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Wounding-induced changes in cellular pressure and localized auxin signalling
spatially coordinate restorative divisions in roots
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 117
year: '2020'
...
---
_id: '7427'
abstract:
- lang: eng
text: Plants, like other multicellular organisms, survive through a delicate balance
between growth and defense against pathogens. Salicylic acid (SA) is a major defense
signal in plants, and the perception mechanism as well as downstream signaling
activating the immune response are known. Here, we identify a parallel SA signaling
that mediates growth attenuation. SA directly binds to A subunits of protein phosphatase
2A (PP2A), inhibiting activity of this complex. Among PP2A targets, the PIN2 auxin
transporter is hyperphosphorylated in response to SA, leading to changed activity
of this important growth regulator. Accordingly, auxin transport and auxin-mediated
root development, including growth, gravitropic response, and lateral root organogenesis,
are inhibited. This study reveals how SA, besides activating immunity, concomitantly
attenuates growth through crosstalk with the auxin distribution network. Further
analysis of this dual role of SA and characterization of additional SA-regulated
PP2A targets will provide further insights into mechanisms maintaining a balance
between growth and defense.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Shigeyuki Betsuyaku (University of Tsukuba), Alison Delong
(Brown University), Xinnian Dong (Duke University), Dolf Weijers (Wageningen University),
Yuelin Zhang (UBC), and Martine Pastuglia (Institut Jean-Pierre Bourgin) for sharing
published materials; Jana Riederer for help with cantharidin physiological analysis;
David Domjan for help with cloning pET28a-PIN2HL; Qing Lu for help with DARTS; Hana
Kozubı´kova´ for technical support on SA derivative synthesis; Zuzana Vondra´ kova´
for technical support with tobacco cells; Lucia Strader (Washington University),
Bert De Rybel (Ghent University), Bartel Vanholme (Ghent University), and Lukas
Mach (BOKU) for helpful discussions; and bioimaging and life science facilities
of IST Austria for continuous support. We gratefully acknowledge the Nottingham
Arabidopsis Stock Center (NASC) for providing T-DNA insertional mutants. The DSC
and SPR instruments were provided by the EQ-BOKU VIBT GmbH and the BOKU Core Facility
for Biomolecular and Cellular Analysis, with help of Irene Schaffner. The research
leading to these results has received funding from the European Union’s Horizon
2020 program (ERC grant agreement no. 742985 to J.F.) and the People Programme (Marie
Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013)
under REA grant agreement no. 291734. S.T. was supported by a European Molecular
Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). O.N.
was supported by the Ministry of Education, Youth and Sports of the Czech Republic
(European Regional Development Fund-Project ‘‘Centre for Experimental Plant Biology’’
no. CZ.02.1.01/0.0/0.0/16_019/0000738). J. Pospısil was supported by European Regional
Development Fund Project ‘‘Centre for Experimental Plant Biology’’\r\n(no. CZ.02.1.01/0.0/0.0/16_019/0000738).
J. Petrasek was supported by EU Operational Programme Prague-Competitiveness (no.
CZ.2.16/3.1.00/21519). "
article_processing_charge: No
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Melinda F
full_name: Abas, Melinda F
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Pavel
full_name: Lasák, Pavel
last_name: Lasák
- first_name: Ivan
full_name: Petřík, Ivan
last_name: Petřík
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Pospíšil, Jiří
last_name: Pospíšil
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tan S, Abas MF, Verstraeten I, et al. Salicylic acid targets protein phosphatase
2A to attenuate growth in plants. Current Biology. 2020;30(3):381-395.e8.
doi:10.1016/j.cub.2019.11.058
apa: Tan, S., Abas, M. F., Verstraeten, I., Glanc, M., Molnar, G., Hajny, J., …
Friml, J. (2020). Salicylic acid targets protein phosphatase 2A to attenuate growth
in plants. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2019.11.058
chicago: Tan, Shutang, Melinda F Abas, Inge Verstraeten, Matous Glanc, Gergely Molnar,
Jakub Hajny, Pavel Lasák, et al. “Salicylic Acid Targets Protein Phosphatase 2A
to Attenuate Growth in Plants.” Current Biology. Cell Press, 2020. https://doi.org/10.1016/j.cub.2019.11.058.
ieee: S. Tan et al., “Salicylic acid targets protein phosphatase 2A to attenuate
growth in plants,” Current Biology, vol. 30, no. 3. Cell Press, p. 381–395.e8,
2020.
ista: Tan S, Abas MF, Verstraeten I, Glanc M, Molnar G, Hajny J, Lasák P, Petřík
I, Russinova E, Petrášek J, Novák O, Pospíšil J, Friml J. 2020. Salicylic acid
targets protein phosphatase 2A to attenuate growth in plants. Current Biology.
30(3), 381–395.e8.
mla: Tan, Shutang, et al. “Salicylic Acid Targets Protein Phosphatase 2A to Attenuate
Growth in Plants.” Current Biology, vol. 30, no. 3, Cell Press, 2020, p.
381–395.e8, doi:10.1016/j.cub.2019.11.058.
short: S. Tan, M.F. Abas, I. Verstraeten, M. Glanc, G. Molnar, J. Hajny, P. Lasák,
I. Petřík, E. Russinova, J. Petrášek, O. Novák, J. Pospíšil, J. Friml, Current
Biology 30 (2020) 381–395.e8.
date_created: 2020-02-02T23:01:00Z
date_published: 2020-02-03T00:00:00Z
date_updated: 2024-03-28T23:30:38Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cub.2019.11.058
ec_funded: 1
external_id:
isi:
- '000511287900018'
pmid:
- '31956021'
file:
- access_level: open_access
checksum: 16f7d51fe28f91c21e4896a2028df40b
content_type: application/pdf
creator: dernst
date_created: 2020-09-22T09:51:28Z
date_updated: 2020-09-22T09:51:28Z
file_id: '8555'
file_name: 2020_CurrentBiology_Tan.pdf
file_size: 5360135
relation: main_file
success: 1
file_date_updated: 2020-09-22T09:51:28Z
has_accepted_license: '1'
intvolume: ' 30'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 381-395.e8
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
grant_number: 723-2015
name: Long Term Fellowship
publication: Current Biology
publication_identifier:
issn:
- '09609822'
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Salicylic acid targets protein phosphatase 2A to attenuate growth in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 30
year: '2020'
...
---
_id: '7500'
abstract:
- lang: eng
text: "Plant survival depends on vascular tissues, which originate in a self‐organizing
manner as strands of cells co‐directionally transporting the plant hormone auxin.
The latter phenomenon (also known as auxin canalization) is classically hypothesized
to be regulated by auxin itself via the effect of this hormone on the polarity
of its own intercellular transport. Correlative observations supported this concept,
but molecular insights remain limited.\r\nIn the current study, we established
an experimental system based on the model Arabidopsis thaliana, which exhibits
auxin transport channels and formation of vasculature strands in response to local
auxin application.\r\nOur methodology permits the genetic analysis of auxin canalization
under controllable experimental conditions. By utilizing this opportunity, we
confirmed the dependence of auxin canalization on a PIN‐dependent auxin transport
and nuclear, TIR1/AFB‐mediated auxin signaling. We also show that leaf venation
and auxin‐mediated PIN repolarization in the root require TIR1/AFB signaling.\r\nFurther
studies based on this experimental system are likely to yield better understanding
of the mechanisms underlying auxin transport polarization in other developmental
contexts."
acknowledgement: We thank Mark Estelle, José M. Alonso and the Arabidopsis Stock Centre
for providing seeds. We acknowledge the core facility CELLIM of CEITEC supported
by the MEYS CR (LM2015062 Czech‐BioImaging) and Plant Sciences Core Facility of
CEITEC Masaryk University for help in generating essential data. This project received
funding from the European Research Council (ERC) under the European Union's Horizon
2020 research and innovation program (grant agreement no. 742985) and the Czech
Science Foundation GAČR (GA13‐40637S and GA18‐26981S) to JF. JH is the recipient
of a DOC Fellowship of the Austrian Academy of Sciences at the Institute of Science
and Technology. The authors declare no competing interests.
article_processing_charge: No
article_type: original
author:
- first_name: E
full_name: Mazur, E
last_name: Mazur
- first_name: Ivan
full_name: Kulik, Ivan
id: F0AB3FCE-02D1-11E9-BD0E-99399A5D3DEB
last_name: Kulik
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mazur E, Kulik I, Hajny J, Friml J. Auxin canalization and vascular tissue
formation by TIR1/AFB-mediated auxin signaling in arabidopsis. New Phytologist.
2020;226(5):1375-1383. doi:10.1111/nph.16446
apa: Mazur, E., Kulik, I., Hajny, J., & Friml, J. (2020). Auxin canalization
and vascular tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis.
New Phytologist. Wiley. https://doi.org/10.1111/nph.16446
chicago: Mazur, E, Ivan Kulik, Jakub Hajny, and Jiří Friml. “Auxin Canalization
and Vascular Tissue Formation by TIR1/AFB-Mediated Auxin Signaling in Arabidopsis.”
New Phytologist. Wiley, 2020. https://doi.org/10.1111/nph.16446.
ieee: E. Mazur, I. Kulik, J. Hajny, and J. Friml, “Auxin canalization and vascular
tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis,” New
Phytologist, vol. 226, no. 5. Wiley, pp. 1375–1383, 2020.
ista: Mazur E, Kulik I, Hajny J, Friml J. 2020. Auxin canalization and vascular
tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis. New Phytologist.
226(5), 1375–1383.
mla: Mazur, E., et al. “Auxin Canalization and Vascular Tissue Formation by TIR1/AFB-Mediated
Auxin Signaling in Arabidopsis.” New Phytologist, vol. 226, no. 5, Wiley,
2020, pp. 1375–83, doi:10.1111/nph.16446.
short: E. Mazur, I. Kulik, J. Hajny, J. Friml, New Phytologist 226 (2020) 1375–1383.
date_created: 2020-02-18T10:03:47Z
date_published: 2020-06-01T00:00:00Z
date_updated: 2024-03-28T23:30:38Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.16446
ec_funded: 1
external_id:
isi:
- '000514939700001'
pmid:
- '31971254'
file:
- access_level: open_access
checksum: 17de728b0205979feb95ce663ba918c2
content_type: application/pdf
creator: dernst
date_created: 2020-11-20T09:32:10Z
date_updated: 2020-11-20T09:32:10Z
file_id: '8781'
file_name: 2020_NewPhytologist_Mazur.pdf
file_size: 2106888
relation: main_file
success: 1
file_date_updated: 2020-11-20T09:32:10Z
has_accepted_license: '1'
intvolume: ' 226'
isi: 1
issue: '5'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1375-1383
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 2699E3D2-B435-11E9-9278-68D0E5697425
grant_number: '25239'
name: Cell surface receptor complexes for PIN polarity and auxin-mediated development
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
status: public
title: Auxin canalization and vascular tissue formation by TIR1/AFB-mediated auxin
signaling in arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 226
year: '2020'
...
---
_id: '8822'
abstract:
- lang: eng
text: "Self-organization is a hallmark of plant development manifested e.g. by intricate
leaf vein patterns, flexible formation of vasculature during organogenesis or
its regeneration following wounding. Spontaneously arising channels transporting
the phytohormone auxin, created by coordinated polar localizations of PIN-FORMED
1 (PIN1) auxin exporter, provide positional cues for these as well as other plant
patterning processes. To find regulators acting downstream of auxin and the TIR1/AFB
auxin signaling pathway essential for PIN1 coordinated polarization during auxin
canalization, we performed microarray experiments. Besides the known components
of general PIN polarity maintenance, such as PID and PIP5K kinases, we identified
and characterized a new regulator of auxin canalization, the transcription factor
WRKY DNA-BINDING PROTEIN 23 (WRKY23).\r\nNext, we designed a subsequent microarray
experiment to further uncover other molecular players, downstream of auxin-TIR1/AFB-WRKY23
involved in the regulation of auxin-mediated PIN repolarization. We identified
a novel and crucial part of the molecular machinery underlying auxin canalization.
The auxin-regulated malectin-type receptor-like kinase CAMEL and the associated
leucine-rich repeat receptor-like kinase CANAR target and directly phosphorylate
PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular
trafficking and auxin-mediated repolarization leading to defects in auxin transport,
ultimately to leaf venation and vasculature regeneration defects. Our results
describe the CAMEL-CANAR receptor complex, which is required for auxin feed-back
on its own transport and thus for coordinated tissue polarization during auxin
canalization."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
citation:
ama: Hajny J. Identification and characterization of the molecular machinery of
auxin-dependent canalization during vasculature formation and regeneration. 2020.
doi:10.15479/AT:ISTA:8822
apa: Hajny, J. (2020). Identification and characterization of the molecular machinery
of auxin-dependent canalization during vasculature formation and regeneration.
Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:8822
chicago: Hajny, Jakub. “Identification and Characterization of the Molecular Machinery
of Auxin-Dependent Canalization during Vasculature Formation and Regeneration.”
Institute of Science and Technology Austria, 2020. https://doi.org/10.15479/AT:ISTA:8822.
ieee: J. Hajny, “Identification and characterization of the molecular machinery
of auxin-dependent canalization during vasculature formation and regeneration,”
Institute of Science and Technology Austria, 2020.
ista: Hajny J. 2020. Identification and characterization of the molecular machinery
of auxin-dependent canalization during vasculature formation and regeneration.
Institute of Science and Technology Austria.
mla: Hajny, Jakub. Identification and Characterization of the Molecular Machinery
of Auxin-Dependent Canalization during Vasculature Formation and Regeneration.
Institute of Science and Technology Austria, 2020, doi:10.15479/AT:ISTA:8822.
short: J. Hajny, Identification and Characterization of the Molecular Machinery
of Auxin-Dependent Canalization during Vasculature Formation and Regeneration,
Institute of Science and Technology Austria, 2020.
date_created: 2020-12-01T12:38:18Z
date_published: 2020-12-01T00:00:00Z
date_updated: 2023-09-19T10:39:33Z
day: '01'
ddc:
- '580'
degree_awarded: PhD
department:
- _id: JiFr
doi: 10.15479/AT:ISTA:8822
file:
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checksum: 210a9675af5e4c78b0b56d920ac82866
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: jhajny
date_created: 2020-12-04T07:27:52Z
date_updated: 2021-07-16T22:30:03Z
embargo_to: open_access
file_id: '8919'
file_name: Jakub Hajný IST Austria final_JH.docx
file_size: 91279806
relation: source_file
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checksum: 1781385b4aa73eba89cc76c6172f71d2
content_type: application/pdf
creator: jhajny
date_created: 2020-12-09T15:04:41Z
date_updated: 2021-12-08T23:30:03Z
embargo: 2021-12-07
file_id: '8933'
file_name: Jakub Hajný IST Austria final_JH-merged without Science.pdf
file_size: 68707697
relation: main_file
file_date_updated: 2021-12-08T23:30:03Z
has_accepted_license: '1'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: '249'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '7427'
relation: part_of_dissertation
status: public
- id: '6260'
relation: part_of_dissertation
status: public
- id: '7500'
relation: part_of_dissertation
status: public
- id: '191'
relation: part_of_dissertation
status: public
- id: '449'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Identification and characterization of the molecular machinery of auxin-dependent
canalization during vasculature formation and regeneration
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2020'
...
---
_id: '8986'
abstract:
- lang: eng
text: 'Flowering plants display the highest diversity among plant species and have
notably shaped terrestrial landscapes. Nonetheless, the evolutionary origin of
their unprecedented morphological complexity remains largely an enigma. Here,
we show that the coevolution of cis-regulatory and coding regions of PIN-FORMED
(PIN) auxin transporters confined their expression to certain cell types and directed
their subcellular localization to particular cell sides, which together enabled
dynamic auxin gradients across tissues critical to the complex architecture of
flowering plants. Extensive intraspecies and interspecies genetic complementation
experiments with PINs from green alga up to flowering plant lineages showed that
PIN genes underwent three subsequent, critical evolutionary innovations and thus
acquired a triple function to regulate the development of three essential components
of the flowering plant Arabidopsis: shoot/root, inflorescence, and floral organ.
Our work highlights the critical role of functional innovations within the PIN
gene family as essential prerequisites for the origin of flowering plants.'
acknowledgement: 'We thank C.Löhne (Botanic Gardens, University of Bonn) for providing
us with A. trichopoda. We would like to thank T.Han, A.Mally (IST, Austria), and
C.Hartinger (University of Oxford) for constructive comment and careful reading.
Funding: The research leading to these results has received funding from the European
Union’s Horizon 2020 Research and Innovation Programme (ERC grant agreement number
742985), Austrian Science Fund (FWF, grant number I 3630-B25), DOC Fellowship of
the Austrian Academy of Sciences, and IST Fellow program. '
article_number: eabc8895
article_processing_charge: No
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Rodriguez Solovey L, Li L, Zhang X, Friml J. Functional innovations
of PIN auxin transporters mark crucial evolutionary transitions during rise of
flowering plants. Science Advances. 2020;6(50). doi:10.1126/sciadv.abc8895
apa: Zhang, Y., Rodriguez Solovey, L., Li, L., Zhang, X., & Friml, J. (2020).
Functional innovations of PIN auxin transporters mark crucial evolutionary transitions
during rise of flowering plants. Science Advances. AAAS. https://doi.org/10.1126/sciadv.abc8895
chicago: Zhang, Yuzhou, Lesia Rodriguez Solovey, Lanxin Li, Xixi Zhang, and Jiří
Friml. “Functional Innovations of PIN Auxin Transporters Mark Crucial Evolutionary
Transitions during Rise of Flowering Plants.” Science Advances. AAAS, 2020.
https://doi.org/10.1126/sciadv.abc8895.
ieee: Y. Zhang, L. Rodriguez Solovey, L. Li, X. Zhang, and J. Friml, “Functional
innovations of PIN auxin transporters mark crucial evolutionary transitions during
rise of flowering plants,” Science Advances, vol. 6, no. 50. AAAS, 2020.
ista: Zhang Y, Rodriguez Solovey L, Li L, Zhang X, Friml J. 2020. Functional innovations
of PIN auxin transporters mark crucial evolutionary transitions during rise of
flowering plants. Science Advances. 6(50), eabc8895.
mla: Zhang, Yuzhou, et al. “Functional Innovations of PIN Auxin Transporters Mark
Crucial Evolutionary Transitions during Rise of Flowering Plants.” Science
Advances, vol. 6, no. 50, eabc8895, AAAS, 2020, doi:10.1126/sciadv.abc8895.
short: Y. Zhang, L. Rodriguez Solovey, L. Li, X. Zhang, J. Friml, Science Advances
6 (2020).
date_created: 2021-01-03T23:01:23Z
date_published: 2020-12-11T00:00:00Z
date_updated: 2024-03-28T23:30:44Z
day: '11'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1126/sciadv.abc8895
ec_funded: 1
external_id:
isi:
- '000599903600014'
pmid:
- '33310852'
file:
- access_level: open_access
checksum: 5ac2500b191c08ef6dab5327f40ff663
content_type: application/pdf
creator: dernst
date_created: 2021-01-07T12:44:33Z
date_updated: 2021-01-07T12:44:33Z
file_id: '8994'
file_name: 2020_ScienceAdvances_Zhang.pdf
file_size: 10578145
relation: main_file
success: 1
file_date_updated: 2021-01-07T12:44:33Z
has_accepted_license: '1'
intvolume: ' 6'
isi: 1
issue: '50'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Science Advances
publication_identifier:
eissn:
- 2375-2548
publication_status: published
publisher: AAAS
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Functional innovations of PIN auxin transporters mark crucial evolutionary
transitions during rise of flowering plants
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 6
year: '2020'
...
---
_id: '8283'
abstract:
- lang: eng
text: 'Drought and salt stress are the main environmental cues affecting the survival,
development, distribution, and yield of crops worldwide. MYB transcription factors
play a crucial role in plants’ biological processes, but the function of pineapple
MYB genes is still obscure. In this study, one of the pineapple MYB transcription
factors, AcoMYB4, was isolated and characterized. The results showed that AcoMYB4
is localized in the cell nucleus, and its expression is induced by low temperature,
drought, salt stress, and hormonal stimulation, especially by abscisic acid (ABA).
Overexpression of AcoMYB4 in rice and Arabidopsis enhanced plant sensitivity to
osmotic stress; it led to an increase in the number stomata on leaf surfaces and
lower germination rate under salt and drought stress. Furthermore, in AcoMYB4
OE lines, the membrane oxidation index, free proline, and soluble sugar contents
were decreased. In contrast, electrolyte leakage and malondialdehyde (MDA) content
increased significantly due to membrane injury, indicating higher sensitivity
to drought and salinity stresses. Besides the above, both the expression level
and activities of several antioxidant enzymes were decreased, indicating lower
antioxidant activity in AcoMYB4 transgenic plants. Moreover, under osmotic stress,
overexpression of AcoMYB4 inhibited ABA biosynthesis through a decrease in the
transcription of genes responsible for ABA synthesis (ABA1 and ABA2) and ABA signal
transduction factor ABI5. These results suggest that AcoMYB4 negatively regulates
osmotic stress by attenuating cellular ABA biosynthesis and signal transduction
pathways. '
acknowledgement: 'We would like to thank the reviewers for their helpful comments
on the original manuscript. '
article_number: '5272'
article_processing_charge: No
article_type: original
author:
- first_name: Huihuang
full_name: Chen, Huihuang
last_name: Chen
- first_name: Linyi
full_name: Lai, Linyi
last_name: Lai
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Liping
full_name: Liu, Liping
last_name: Liu
- first_name: Bello Hassan
full_name: Jakada, Bello Hassan
last_name: Jakada
- first_name: Youmei
full_name: Huang, Youmei
last_name: Huang
- first_name: Qing
full_name: He, Qing
last_name: He
- first_name: Mengnan
full_name: Chai, Mengnan
last_name: Chai
- first_name: Xiaoping
full_name: Niu, Xiaoping
last_name: Niu
- first_name: Yuan
full_name: Qin, Yuan
last_name: Qin
citation:
ama: Chen H, Lai L, Li L, et al. AcoMYB4, an Ananas comosus L. MYB transcription
factor, functions in osmotic stress through negative regulation of ABA signaling.
International Journal of Molecular Sciences. 2020;21(16). doi:10.3390/ijms21165727
apa: Chen, H., Lai, L., Li, L., Liu, L., Jakada, B. H., Huang, Y., … Qin, Y. (2020).
AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress
through negative regulation of ABA signaling. International Journal of Molecular
Sciences. MDPI. https://doi.org/10.3390/ijms21165727
chicago: Chen, Huihuang, Linyi Lai, Lanxin Li, Liping Liu, Bello Hassan Jakada,
Youmei Huang, Qing He, Mengnan Chai, Xiaoping Niu, and Yuan Qin. “AcoMYB4, an
Ananas Comosus L. MYB Transcription Factor, Functions in Osmotic Stress through
Negative Regulation of ABA Signaling.” International Journal of Molecular Sciences.
MDPI, 2020. https://doi.org/10.3390/ijms21165727.
ieee: H. Chen et al., “AcoMYB4, an Ananas comosus L. MYB transcription factor,
functions in osmotic stress through negative regulation of ABA signaling,” International
Journal of Molecular Sciences, vol. 21, no. 16. MDPI, 2020.
ista: Chen H, Lai L, Li L, Liu L, Jakada BH, Huang Y, He Q, Chai M, Niu X, Qin Y.
2020. AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic
stress through negative regulation of ABA signaling. International Journal of
Molecular Sciences. 21(16), 5272.
mla: Chen, Huihuang, et al. “AcoMYB4, an Ananas Comosus L. MYB Transcription Factor,
Functions in Osmotic Stress through Negative Regulation of ABA Signaling.” International
Journal of Molecular Sciences, vol. 21, no. 16, 5272, MDPI, 2020, doi:10.3390/ijms21165727.
short: H. Chen, L. Lai, L. Li, L. Liu, B.H. Jakada, Y. Huang, Q. He, M. Chai, X.
Niu, Y. Qin, International Journal of Molecular Sciences 21 (2020).
date_created: 2020-08-24T06:24:03Z
date_published: 2020-08-10T00:00:00Z
date_updated: 2024-03-28T23:30:44Z
day: '10'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.3390/ijms21165727
external_id:
isi:
- '000565090300001'
pmid:
- '32785037'
file:
- access_level: open_access
checksum: 03b039244e6ae80580385fd9f577e2b2
content_type: application/pdf
creator: cziletti
date_created: 2020-08-25T09:53:50Z
date_updated: 2020-08-25T09:53:50Z
file_id: '8292'
file_name: 2020_IntMolecSciences_Chen.pdf
file_size: 5718755
relation: main_file
success: 1
file_date_updated: 2020-08-25T09:53:50Z
has_accepted_license: '1'
intvolume: ' 21'
isi: 1
issue: '16'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- '14220067'
issn:
- '16616596'
publication_status: published
publisher: MDPI
quality_controlled: '1'
related_material:
record:
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic
stress through negative regulation of ABA signaling
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 21
year: '2020'
...
---
_id: '8139'
abstract:
- lang: eng
text: 'Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated
in many aspects of plant growth, development, intra- and inter-cellular signaling,
nutrient uptake and pathogen defense. Despite these significant roles, little
is known about the precise molecular details of how it functions in planta. In
order to facilitate the direct quantitative study of plant CME, here we review
current routinely used methods and present refined, standardized quantitative
imaging protocols which allow the detailed characterization of CME at multiple
scales in plant tissues. These include: (i) an efficient electron microscopy protocol
for the imaging of Arabidopsis CME vesicles in situ, thus providing a method for
the detailed characterization of the ultra-structure of clathrin-coated vesicles;
(ii) a detailed protocol and analysis for quantitative live-cell fluorescence
microscopy to precisely examine the temporal interplay of endocytosis components
during single CME events; (iii) a semi-automated analysis to allow the quantitative
characterization of global internalization of cargos in whole plant tissues; and
(iv) an overview and validation of useful genetic and pharmacological tools to
interrogate the molecular mechanisms and function of CME in intact plant samples.'
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
acknowledgement: "This paper is dedicated to the memory of Christien Merrifield. He
pioneered quantitative\r\nimaging approaches in mammalian CME and his mentorship
inspired the development of all\r\nthe analysis methods presented here. His joy
in research, pure scientific curiosity and\r\nmicroscopy excellence remain a constant
inspiration. We thank Daniel Van Damme for gifting\r\nus the CLC2-GFP x TPLATE-TagRFP
plants used in this manuscript. We further thank the\r\nScientific Service Units
at IST Austria; specifically, the Electron Microscopy Facility for\r\ntechnical
assistance (in particular Vanessa Zheden) and the BioImaging Facility BioImaging\r\nFacility
for access to equipment. "
article_number: jcs248062
article_processing_charge: No
article_type: original
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: G
full_name: Vert, G
last_name: Vert
- first_name: SY
full_name: Bednarek, SY
last_name: Bednarek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Johnson AJ, Gnyliukh N, Kaufmann W, et al. Experimental toolbox for quantitative
evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal
of Cell Science. 2020;133(15). doi:10.1242/jcs.248062
apa: Johnson, A. J., Gnyliukh, N., Kaufmann, W., Narasimhan, M., Vert, G., Bednarek,
S., & Friml, J. (2020). Experimental toolbox for quantitative evaluation of
clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell
Science. The Company of Biologists. https://doi.org/10.1242/jcs.248062
chicago: Johnson, Alexander J, Nataliia Gnyliukh, Walter Kaufmann, Madhumitha Narasimhan,
G Vert, SY Bednarek, and Jiří Friml. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science. The Company of Biologists, 2020. https://doi.org/10.1242/jcs.248062.
ieee: A. J. Johnson et al., “Experimental toolbox for quantitative evaluation
of clathrin-mediated endocytosis in the plant model Arabidopsis,” Journal of
Cell Science, vol. 133, no. 15. The Company of Biologists, 2020.
ista: Johnson AJ, Gnyliukh N, Kaufmann W, Narasimhan M, Vert G, Bednarek S, Friml
J. 2020. Experimental toolbox for quantitative evaluation of clathrin-mediated
endocytosis in the plant model Arabidopsis. Journal of Cell Science. 133(15),
jcs248062.
mla: Johnson, Alexander J., et al. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science, vol. 133, no. 15, jcs248062, The Company of Biologists, 2020,
doi:10.1242/jcs.248062.
short: A.J. Johnson, N. Gnyliukh, W. Kaufmann, M. Narasimhan, G. Vert, S. Bednarek,
J. Friml, Journal of Cell Science 133 (2020).
date_created: 2020-07-21T08:58:19Z
date_published: 2020-08-06T00:00:00Z
date_updated: 2023-12-01T13:51:07Z
day: '06'
ddc:
- '575'
department:
- _id: JiFr
- _id: EM-Fac
doi: 10.1242/jcs.248062
ec_funded: 1
external_id:
isi:
- '000561047900021'
pmid:
- '32616560'
file:
- access_level: open_access
checksum: 2d11f79a0b4e0a380fb002b933da331a
content_type: application/pdf
creator: ajohnson
date_created: 2020-11-26T17:12:51Z
date_updated: 2021-08-08T22:30:03Z
embargo: 2021-08-07
file_id: '8815'
file_name: 2020 - Johnson - JSC - plant CME toolbox.pdf
file_size: 15150403
relation: main_file
file_date_updated: 2021-08-08T22:30:03Z
has_accepted_license: '1'
intvolume: ' 133'
isi: 1
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
record:
- id: '14510'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis
in the plant model Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 133
year: '2020'
...
---
_id: '5908'
abstract:
- lang: eng
text: The interorganelle communication mediated by membrane contact sites (MCSs)
is an evolutionary hallmark of eukaryotic cells. MCS connections enable the nonvesicular
exchange of information between organelles and allow them to coordinate responses
to changing cellular environments. In plants, the importance of MCS components
in the responses to environmental stress has been widely established, but the
molecular mechanisms regulating interorganelle connectivity during stress still
remain opaque. In this report, we use the model plant Arabidopsis thaliana to
show that ionic stress increases endoplasmic reticulum (ER)–plasma membrane (PM)
connectivity by promoting the cortical expansion of synaptotagmin 1 (SYT1)-enriched
ER–PM contact sites (S-EPCSs). We define differential roles for the cortical cytoskeleton
in the regulation of S-EPCS dynamics and ER–PM connectivity, and we identify the
accumulation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] at the PM as
a molecular signal associated with the ER–PM connectivity changes. Our study highlights
the functional conservation of EPCS components and PM phosphoinositides as modulators
of ER–PM connectivity in eukaryotes, and uncovers unique aspects of the spatiotemporal
regulation of ER–PM connectivity in plants.
article_processing_charge: No
article_type: original
author:
- first_name: Eunkyoung
full_name: Lee, Eunkyoung
last_name: Lee
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jessica
full_name: Pérez-Sancho, Jessica
last_name: Pérez-Sancho
- first_name: Francisco
full_name: Benitez-Fuente, Francisco
last_name: Benitez-Fuente
- first_name: Matthew
full_name: Strelau, Matthew
last_name: Strelau
- first_name: Alberto P.
full_name: Macho, Alberto P.
last_name: Macho
- first_name: Miguel A.
full_name: Botella, Miguel A.
last_name: Botella
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Abel
full_name: Rosado, Abel
last_name: Rosado
citation:
ama: Lee E, Vanneste S, Pérez-Sancho J, et al. Ionic stress enhances ER–PM connectivity
via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. Proceedings
of the National Academy of Sciences of the United States of America. 2019;116(4):1420-1429.
doi:10.1073/pnas.1818099116
apa: Lee, E., Vanneste, S., Pérez-Sancho, J., Benitez-Fuente, F., Strelau, M., Macho,
A. P., … Rosado, A. (2019). Ionic stress enhances ER–PM connectivity via phosphoinositide-associated
SYT1 contact site expansion in Arabidopsis. Proceedings of the National Academy
of Sciences of the United States of America. National Academy of Sciences.
https://doi.org/10.1073/pnas.1818099116
chicago: Lee, Eunkyoung, Steffen Vanneste, Jessica Pérez-Sancho, Francisco Benitez-Fuente,
Matthew Strelau, Alberto P. Macho, Miguel A. Botella, Jiří Friml, and Abel Rosado.
“Ionic Stress Enhances ER–PM Connectivity via Phosphoinositide-Associated SYT1
Contact Site Expansion in Arabidopsis.” Proceedings of the National Academy
of Sciences of the United States of America. National Academy of Sciences,
2019. https://doi.org/10.1073/pnas.1818099116.
ieee: E. Lee et al., “Ionic stress enhances ER–PM connectivity via phosphoinositide-associated
SYT1 contact site expansion in Arabidopsis,” Proceedings of the National Academy
of Sciences of the United States of America, vol. 116, no. 4. National Academy
of Sciences, pp. 1420–1429, 2019.
ista: Lee E, Vanneste S, Pérez-Sancho J, Benitez-Fuente F, Strelau M, Macho AP,
Botella MA, Friml J, Rosado A. 2019. Ionic stress enhances ER–PM connectivity
via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. Proceedings
of the National Academy of Sciences of the United States of America. 116(4), 1420–1429.
mla: Lee, Eunkyoung, et al. “Ionic Stress Enhances ER–PM Connectivity via Phosphoinositide-Associated
SYT1 Contact Site Expansion in Arabidopsis.” Proceedings of the National Academy
of Sciences of the United States of America, vol. 116, no. 4, National Academy
of Sciences, 2019, pp. 1420–29, doi:10.1073/pnas.1818099116.
short: E. Lee, S. Vanneste, J. Pérez-Sancho, F. Benitez-Fuente, M. Strelau, A.P.
Macho, M.A. Botella, J. Friml, A. Rosado, Proceedings of the National Academy
of Sciences of the United States of America 116 (2019) 1420–1429.
date_created: 2019-02-03T22:59:14Z
date_published: 2019-01-22T00:00:00Z
date_updated: 2023-08-24T14:31:09Z
day: '22'
department:
- _id: JiFr
doi: 10.1073/pnas.1818099116
external_id:
isi:
- '000456336100050'
pmid:
- '30610176'
intvolume: ' 116'
isi: 1
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1073/pnas.1818099116
month: '01'
oa: 1
oa_version: Published Version
page: 1420-1429
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1
contact site expansion in Arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 116
year: '2019'
...
---
_id: '6023'
abstract:
- lang: eng
text: Multicellular development requires coordinated cell polarization relative
to body axes, and translation to oriented cell division 1–3 . In plants, it is
unknown how cell polarities are connected to organismal axes and translated to
division. Here, we identify Arabidopsis SOSEKI proteins that integrate apical–basal
and radial organismal axes to localize to polar cell edges. Localization does
not depend on tissue context, requires cell wall integrity and is defined by a
transferrable, protein-specific motif. A Domain of Unknown Function in SOSEKI
proteins resembles the DIX oligomerization domain in the animal Dishevelled polarity
regulator. The DIX-like domain self-interacts and is required for edge localization
and for influencing division orientation, together with a second domain that defines
the polar membrane domain. Our work shows that SOSEKI proteins locally interpret
global polarity cues and can influence cell division orientation. Furthermore,
this work reveals that, despite fundamental differences, cell polarity mechanisms
in plants and animals converge on a similar protein domain.
article_processing_charge: No
author:
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Alja
full_name: Van Der Schuren, Alja
last_name: Van Der Schuren
- first_name: Maritza
full_name: Van Dop, Maritza
last_name: Van Dop
- first_name: Luc
full_name: Van Galen, Luc
last_name: Van Galen
- first_name: Shunsuke
full_name: Saiga, Shunsuke
last_name: Saiga
- first_name: Milad
full_name: Adibi, Milad
last_name: Adibi
- first_name: Barbara
full_name: Möller, Barbara
last_name: Möller
- first_name: Colette A.
full_name: Ten Hove, Colette A.
last_name: Ten Hove
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Richard
full_name: Smith, Richard
last_name: Smith
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
citation:
ama: Yoshida S, Van Der Schuren A, Van Dop M, et al. A SOSEKI-based coordinate system
interprets global polarity cues in arabidopsis. Nature Plants. 2019;5(2):160-166.
doi:10.1038/s41477-019-0363-6
apa: Yoshida, S., Van Der Schuren, A., Van Dop, M., Van Galen, L., Saiga, S., Adibi,
M., … Weijers, D. (2019). A SOSEKI-based coordinate system interprets global polarity
cues in arabidopsis. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-019-0363-6
chicago: Yoshida, Saiko, Alja Van Der Schuren, Maritza Van Dop, Luc Van Galen, Shunsuke
Saiga, Milad Adibi, Barbara Möller, et al. “A SOSEKI-Based Coordinate System Interprets
Global Polarity Cues in Arabidopsis.” Nature Plants. Springer Nature, 2019.
https://doi.org/10.1038/s41477-019-0363-6.
ieee: S. Yoshida et al., “A SOSEKI-based coordinate system interprets global
polarity cues in arabidopsis,” Nature Plants, vol. 5, no. 2. Springer Nature,
pp. 160–166, 2019.
ista: Yoshida S, Van Der Schuren A, Van Dop M, Van Galen L, Saiga S, Adibi M, Möller
B, Ten Hove CA, Marhavý P, Smith R, Friml J, Weijers D. 2019. A SOSEKI-based coordinate
system interprets global polarity cues in arabidopsis. Nature Plants. 5(2), 160–166.
mla: Yoshida, Saiko, et al. “A SOSEKI-Based Coordinate System Interprets Global
Polarity Cues in Arabidopsis.” Nature Plants, vol. 5, no. 2, Springer Nature,
2019, pp. 160–66, doi:10.1038/s41477-019-0363-6.
short: S. Yoshida, A. Van Der Schuren, M. Van Dop, L. Van Galen, S. Saiga, M. Adibi,
B. Möller, C.A. Ten Hove, P. Marhavý, R. Smith, J. Friml, D. Weijers, Nature Plants
5 (2019) 160–166.
date_created: 2019-02-17T22:59:21Z
date_published: 2019-02-08T00:00:00Z
date_updated: 2023-08-24T14:46:47Z
day: '08'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1038/s41477-019-0363-6
ec_funded: 1
external_id:
isi:
- '000460479600014'
intvolume: ' 5'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.biorxiv.org/content/10.1101/479113v1.abstract
month: '02'
oa: 1
oa_version: Submitted Version
page: 160-166
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Nature Plants
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 5
year: '2019'
...
---
_id: '6104'
abstract:
- lang: eng
text: Abiotic stress poses constant challenges for plant survival and is a serious
problem for global agricultural productivity. On a molecular level, stress conditions
result in elevation of reactive oxygen species (ROS) production causing oxidative
stress associated with oxidation of proteins and nucleic acids as well as impairment
of membrane functions. Adaptation of root growth to ROS accumulation is facilitated
through modification of auxin and cytokinin hormone homeostasis. Here, we report
that in Arabidopsis root meristem, ROS-induced changes of auxin levels correspond
to decreased abundance of PIN auxin efflux carriers at the plasma membrane (PM).
Specifically, increase in H2O2 levels affects PIN2 endocytic recycling. We show
that the PIN2 intracellular trafficking during adaptation to oxidative stress
requires the function of the ADP-ribosylation factor (ARF)-guanine-nucleotide
exchange factor (GEF) BEN1, an actin-associated regulator of the trafficking from
the PM to early endosomes and, presumably, indirectly, trafficking to the vacuoles.
We propose that H2O2 levels affect the actin dynamics thus modulating ARF-GEF-dependent
trafficking of PIN2. This mechanism provides a way how root growth acclimates
to stress and adapts to a changing environment.
article_processing_charge: No
author:
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Agnieszka
full_name: Bielach, Agnieszka
last_name: Bielach
- first_name: Prashanth
full_name: Tamizhselvan, Prashanth
last_name: Tamizhselvan
- first_name: Sharmila
full_name: Madhavan, Sharmila
last_name: Madhavan
- first_name: Eman Elrefaay
full_name: Ryad, Eman Elrefaay
last_name: Ryad
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Petre
full_name: Dobrev, Petre
last_name: Dobrev
- first_name: Radomira
full_name: Vanková, Radomira
last_name: Vanková
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Vanesa B.
full_name: Tognetti, Vanesa B.
last_name: Tognetti
citation:
ama: Zwiewka M, Bielach A, Tamizhselvan P, et al. Root adaptation to H2O2-induced
oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking.
Plant and Cell Physiology. 2019;60(2):255-273. doi:10.1093/pcp/pcz001
apa: Zwiewka, M., Bielach, A., Tamizhselvan, P., Madhavan, S., Ryad, E. E., Tan,
S., … Tognetti, V. B. (2019). Root adaptation to H2O2-induced oxidative stress
by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking. Plant and Cell
Physiology. Oxford University Press. https://doi.org/10.1093/pcp/pcz001
chicago: Zwiewka, Marta, Agnieszka Bielach, Prashanth Tamizhselvan, Sharmila Madhavan,
Eman Elrefaay Ryad, Shutang Tan, Mónika Hrtyan, et al. “Root Adaptation to H2O2-Induced
Oxidative Stress by ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.”
Plant and Cell Physiology. Oxford University Press, 2019. https://doi.org/10.1093/pcp/pcz001.
ieee: M. Zwiewka et al., “Root adaptation to H2O2-induced oxidative stress
by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking,” Plant and Cell
Physiology, vol. 60, no. 2. Oxford University Press, pp. 255–273, 2019.
ista: Zwiewka M, Bielach A, Tamizhselvan P, Madhavan S, Ryad EE, Tan S, Hrtyan M,
Dobrev P, Vanková R, Friml J, Tognetti VB. 2019. Root adaptation to H2O2-induced
oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking.
Plant and Cell Physiology. 60(2), 255–273.
mla: Zwiewka, Marta, et al. “Root Adaptation to H2O2-Induced Oxidative Stress by
ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.” Plant and Cell Physiology,
vol. 60, no. 2, Oxford University Press, 2019, pp. 255–73, doi:10.1093/pcp/pcz001.
short: M. Zwiewka, A. Bielach, P. Tamizhselvan, S. Madhavan, E.E. Ryad, S. Tan,
M. Hrtyan, P. Dobrev, R. Vanková, J. Friml, V.B. Tognetti, Plant and Cell Physiology
60 (2019) 255–273.
date_created: 2019-03-17T22:59:14Z
date_published: 2019-02-01T00:00:00Z
date_updated: 2023-08-25T08:05:28Z
day: '01'
department:
- _id: JiFr
doi: 10.1093/pcp/pcz001
external_id:
isi:
- '000459634300002'
pmid:
- '30668780'
intvolume: ' 60'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 255-273
pmid: 1
publication: Plant and Cell Physiology
publication_identifier:
eissn:
- 1471-9053
issn:
- 0032-0781
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated
PIN2 trafficking
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 60
year: '2019'
...
---
_id: '6262'
abstract:
- lang: eng
text: "Gravitropism is an adaptive response that orients plant growth parallel to
the gravity vector. Asymmetric\r\ndistribution of the phytohormone auxin is a
necessary prerequisite to the tropic bending both in roots and\r\nshoots. During
hypocotyl gravitropic response, the PIN3 auxin transporter polarizes within gravity-sensing\r\ncells
to redirect intercellular auxin fluxes. First gravity-induced PIN3 polarization
to the bottom cell mem-\r\nbranes leads to the auxin accumulation at the lower
side of the organ, initiating bending and, later, auxin\r\nfeedback-mediated repolarization
restores symmetric auxin distribution to terminate bending. Here, we per-\r\nformed
a forward genetic screen to identify regulators of both PIN3 polarization events
during gravitropic\r\nresponse. We searched for mutants with defective PIN3 polarizations
based on easy-to-score morphological\r\noutputs of decreased or increased gravity-induced
hypocotyl bending. We identified the number of\r\nhypocotyl reduced bending (hrb)
and hypocotyl hyperbending (hhb) mutants, revealing that reduced bending corre-\r\nlated
typically with defective gravity-induced PIN3 relocation whereas all analyzed
hhb mutants showed\r\ndefects in the second, auxin-mediated PIN3 relocation. Next-generation
sequencing-aided mutation map-\r\nping identified several candidate genes, including
SCARECROW and ACTIN2, revealing roles of endodermis\r\nspecification and actin
cytoskeleton in the respective gravity- and auxin-induced PIN polarization events.\r\nThe
hypocotyl gravitropism screen thus promises to provide novel insights into mechanisms
underlying cell\r\npolarity and plant adaptive development."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Petr
full_name: Valošek, Petr
id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87
last_name: Valošek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rakusová H, Han H, Valošek P, Friml J. Genetic screen for factors mediating
PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. The Plant
Journal. 2019;98(6):1048-1059. doi:10.1111/tpj.14301
apa: Rakusová, H., Han, H., Valošek, P., & Friml, J. (2019). Genetic screen
for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana
hypocotyls. The Plant Journal. Wiley. https://doi.org/10.1111/tpj.14301
chicago: Rakusová, Hana, Huibin Han, Petr Valošek, and Jiří Friml. “Genetic Screen
for Factors Mediating PIN Polarization in Gravistimulated Arabidopsis Thaliana
Hypocotyls.” The Plant Journal. Wiley, 2019. https://doi.org/10.1111/tpj.14301.
ieee: H. Rakusová, H. Han, P. Valošek, and J. Friml, “Genetic screen for factors
mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls,”
The Plant Journal, vol. 98, no. 6. Wiley, pp. 1048–1059, 2019.
ista: Rakusová H, Han H, Valošek P, Friml J. 2019. Genetic screen for factors mediating
PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. The Plant
Journal. 98(6), 1048–1059.
mla: Rakusová, Hana, et al. “Genetic Screen for Factors Mediating PIN Polarization
in Gravistimulated Arabidopsis Thaliana Hypocotyls.” The Plant Journal,
vol. 98, no. 6, Wiley, 2019, pp. 1048–59, doi:10.1111/tpj.14301.
short: H. Rakusová, H. Han, P. Valošek, J. Friml, The Plant Journal 98 (2019) 1048–1059.
date_created: 2019-04-09T08:46:44Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2023-08-25T10:11:03Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/tpj.14301
ec_funded: 1
external_id:
isi:
- '000473644100008'
pmid:
- '30821050'
file:
- access_level: open_access
checksum: ad3b5e270b67ba2a45f894ce3be27920
content_type: application/pdf
creator: dernst
date_created: 2019-04-15T09:38:43Z
date_updated: 2020-07-14T12:47:25Z
file_id: '6304'
file_name: 2019_PlantJournal_Rakusov.pdf
file_size: 1383100
relation: main_file
file_date_updated: 2020-07-14T12:47:25Z
has_accepted_license: '1'
intvolume: ' 98'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1048-1059
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: The Plant Journal
publication_identifier:
eissn:
- 1365-313x
issn:
- 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis
thaliana hypocotyls
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 98
year: '2019'
...
---
_id: '6261'
abstract:
- lang: eng
text: Nitrate regulation of root stem cell activity is auxin-dependent.
article_processing_charge: No
article_type: letter_note
author:
- first_name: Y
full_name: Wang, Y
last_name: Wang
- first_name: Z
full_name: Gong, Z
last_name: Gong
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: J
full_name: Zhang, J
last_name: Zhang
citation:
ama: Wang Y, Gong Z, Friml J, Zhang J. Nitrate modulates the differentiation of
root distal stem cells. Plant Physiology. 2019;180(1):22-25. doi:10.1104/pp.18.01305
apa: Wang, Y., Gong, Z., Friml, J., & Zhang, J. (2019). Nitrate modulates the
differentiation of root distal stem cells. Plant Physiology. ASPB. https://doi.org/10.1104/pp.18.01305
chicago: Wang, Y, Z Gong, Jiří Friml, and J Zhang. “Nitrate Modulates the Differentiation
of Root Distal Stem Cells.” Plant Physiology. ASPB, 2019. https://doi.org/10.1104/pp.18.01305.
ieee: Y. Wang, Z. Gong, J. Friml, and J. Zhang, “Nitrate modulates the differentiation
of root distal stem cells,” Plant Physiology, vol. 180, no. 1. ASPB, pp.
22–25, 2019.
ista: Wang Y, Gong Z, Friml J, Zhang J. 2019. Nitrate modulates the differentiation
of root distal stem cells. Plant Physiology. 180(1), 22–25.
mla: Wang, Y., et al. “Nitrate Modulates the Differentiation of Root Distal Stem
Cells.” Plant Physiology, vol. 180, no. 1, ASPB, 2019, pp. 22–25, doi:10.1104/pp.18.01305.
short: Y. Wang, Z. Gong, J. Friml, J. Zhang, Plant Physiology 180 (2019) 22–25.
date_created: 2019-04-09T08:46:17Z
date_published: 2019-05-01T00:00:00Z
date_updated: 2023-08-25T10:10:23Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.18.01305
external_id:
isi:
- '000466860800010'
pmid:
- '30787134'
intvolume: ' 180'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1104/pp.18.01305
month: '05'
oa: 1
oa_version: Published Version
page: 22-25
pmid: 1
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: ASPB
quality_controlled: '1'
scopus_import: '1'
status: public
title: Nitrate modulates the differentiation of root distal stem cells
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 180
year: '2019'
...
---
_id: '6504'
abstract:
- lang: eng
text: "Root gravitropism is one of the most important processes allowing plant adaptation
to the land environment. Auxin plays a central role in mediating root gravitropism,
but how auxin contributes to gravitational perception and the subsequent response
is still unclear.\r\n\r\nHere, we showed that the local auxin maximum/gradient
within the root apex, which is generated by the PIN directional auxin transporters,
regulates the expression of three key starch granule synthesis genes, SS4, PGM
and ADG1, which in turn influence the accumulation of starch granules that serve
as a statolith perceiving gravity.\r\n\r\nMoreover, using the cvxIAA‐ccvTIR1 system,
we also showed that TIR1‐mediated auxin signaling is required for starch granule
formation and gravitropic response within root tips. In addition, axr3 mutants
showed reduced auxin‐mediated starch granule accumulation and disruption of gravitropism
within the root apex.\r\n\r\nOur results indicate that auxin‐mediated statolith
production relies on the TIR1/AFB‐AXR3‐mediated auxin signaling pathway. In summary,
we propose a dual role for auxin in gravitropism: the regulation of both gravity
perception and response."
article_processing_charge: No
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: P
full_name: He, P
last_name: He
- first_name: X
full_name: Ma, X
last_name: Ma
- first_name: Z
full_name: Yang, Z
last_name: Yang
- first_name: C
full_name: Pang, C
last_name: Pang
- first_name: J
full_name: Yu, J
last_name: Yu
- first_name: G
full_name: Wang, G
last_name: Wang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Xiao, G
last_name: Xiao
citation:
ama: Zhang Y, He P, Ma X, et al. Auxin-mediated statolith production for root gravitropism.
New Phytologist. 2019;224(2):761-774. doi:10.1111/nph.15932
apa: Zhang, Y., He, P., Ma, X., Yang, Z., Pang, C., Yu, J., … Xiao, G. (2019). Auxin-mediated
statolith production for root gravitropism. New Phytologist. Wiley. https://doi.org/10.1111/nph.15932
chicago: Zhang, Yuzhou, P He, X Ma, Z Yang, C Pang, J Yu, G Wang, Jiří Friml, and
G Xiao. “Auxin-Mediated Statolith Production for Root Gravitropism.” New Phytologist.
Wiley, 2019. https://doi.org/10.1111/nph.15932.
ieee: Y. Zhang et al., “Auxin-mediated statolith production for root gravitropism,”
New Phytologist, vol. 224, no. 2. Wiley, pp. 761–774, 2019.
ista: Zhang Y, He P, Ma X, Yang Z, Pang C, Yu J, Wang G, Friml J, Xiao G. 2019.
Auxin-mediated statolith production for root gravitropism. New Phytologist. 224(2),
761–774.
mla: Zhang, Yuzhou, et al. “Auxin-Mediated Statolith Production for Root Gravitropism.”
New Phytologist, vol. 224, no. 2, Wiley, 2019, pp. 761–74, doi:10.1111/nph.15932.
short: Y. Zhang, P. He, X. Ma, Z. Yang, C. Pang, J. Yu, G. Wang, J. Friml, G. Xiao,
New Phytologist 224 (2019) 761–774.
date_created: 2019-05-28T14:33:26Z
date_published: 2019-10-01T00:00:00Z
date_updated: 2023-08-28T08:40:13Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.15932
external_id:
isi:
- '000487184200024'
pmid:
- '31111487'
file:
- access_level: open_access
checksum: 6488243334538f5c39099a701cbf76b9
content_type: application/pdf
creator: dernst
date_created: 2020-10-14T08:59:33Z
date_updated: 2020-10-14T08:59:33Z
file_id: '8661'
file_name: 2019_NewPhytologist_Zhang_accepted.pdf
file_size: 1099061
relation: main_file
success: 1
file_date_updated: 2020-10-14T08:59:33Z
has_accepted_license: '1'
intvolume: ' 224'
isi: 1
issue: '2'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 761-774
pmid: 1
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin-mediated statolith production for root gravitropism
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 224
year: '2019'
...
---
_id: '6611'
abstract:
- lang: eng
text: 'Cell polarity is crucial for the coordinated development of all multicellular
organisms. In plants, this is exemplified by the PIN-FORMED (PIN) efflux carriers
of the phytohormone auxin: The polar subcellular localization of the PINs is instructive
to the directional intercellular auxin transport, and thus to a plethora of auxin-regulated
growth and developmental processes. Despite its importance, the regulation of
PIN polar subcellular localization remains poorly understood. Here, we have employed
advanced live-cell imaging techniques to study the roles of microtubules and actin
microfilaments in the establishment of apical polar localization of PIN2 in the
epidermis of the Arabidopsis root meristem. We report that apical PIN2 polarity
requires neither intact actin microfilaments nor microtubules, suggesting that
the primary spatial cue for polar PIN distribution is likely independent of cytoskeleton-guided
endomembrane trafficking.'
acknowledged_ssus:
- _id: Bio
article_number: '222'
article_processing_charge: No
author:
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Glanc M, Fendrych M, Friml J. PIN2 polarity establishment in arabidopsis in
the absence of an intact cytoskeleton. Biomolecules. 2019;9(6). doi:10.3390/biom9060222
apa: Glanc, M., Fendrych, M., & Friml, J. (2019). PIN2 polarity establishment
in arabidopsis in the absence of an intact cytoskeleton. Biomolecules.
MDPI. https://doi.org/10.3390/biom9060222
chicago: Glanc, Matous, Matyas Fendrych, and Jiří Friml. “PIN2 Polarity Establishment
in Arabidopsis in the Absence of an Intact Cytoskeleton.” Biomolecules.
MDPI, 2019. https://doi.org/10.3390/biom9060222.
ieee: M. Glanc, M. Fendrych, and J. Friml, “PIN2 polarity establishment in arabidopsis
in the absence of an intact cytoskeleton,” Biomolecules, vol. 9, no. 6.
MDPI, 2019.
ista: Glanc M, Fendrych M, Friml J. 2019. PIN2 polarity establishment in arabidopsis
in the absence of an intact cytoskeleton. Biomolecules. 9(6), 222.
mla: Glanc, Matous, et al. “PIN2 Polarity Establishment in Arabidopsis in the Absence
of an Intact Cytoskeleton.” Biomolecules, vol. 9, no. 6, 222, MDPI, 2019,
doi:10.3390/biom9060222.
short: M. Glanc, M. Fendrych, J. Friml, Biomolecules 9 (2019).
date_created: 2019-07-07T21:59:21Z
date_published: 2019-06-07T00:00:00Z
date_updated: 2023-08-28T12:30:24Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/biom9060222
ec_funded: 1
external_id:
isi:
- '000475301500018'
pmid:
- '31181636'
file:
- access_level: open_access
checksum: 1ce1bd36038fe5381057a1bcc6760083
content_type: application/pdf
creator: kschuh
date_created: 2019-07-08T15:46:32Z
date_updated: 2020-07-14T12:47:34Z
file_id: '6625'
file_name: biomolecules-2019-Matous.pdf
file_size: 1066773
relation: main_file
file_date_updated: 2020-07-14T12:47:34Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Biomolecules
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: PIN2 polarity establishment in arabidopsis in the absence of an intact cytoskeleton
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2019'
...
---
_id: '6778'
abstract:
- lang: eng
text: "An important adaptation during colonization of land by plants is gravitropic
growth of roots, which enabled roots to reach water and nutrients, and firmly
anchor plants in the ground. Here we provide insights into the evolution of an
efficient root gravitropic mechanism in the seed plants. Architectural innovation,
with gravity perception constrained in the root tips\r\nalong with a shootward
transport route for the phytohormone auxin, appeared only upon the emergence of
seed plants. Interspecies complementation and protein domain swapping revealed
functional innovations within the PIN family of auxin transporters leading to
the evolution of gravitropism-specific PINs. The unique apical/shootward subcellular
localization of PIN proteins is the major evolutionary innovation that connected
the anatomically separated sites of gravity perception and growth response via
the mobile auxin signal. We conclude that the crucial anatomical and functional
components emerged hand-in-hand to facilitate the evolution of fast gravitropic
response, which is one of the major adaptations of seed plants to dry land."
article_number: '3480'
article_processing_charge: No
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: G
full_name: Xiao, G
last_name: Xiao
- first_name: X
full_name: Wang, X
last_name: Wang
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zhang Y, Xiao G, Wang X, Zhang X, Friml J. Evolution of fast root gravitropism
in seed plants. Nature Communications. 2019;10. doi:10.1038/s41467-019-11471-8
apa: Zhang, Y., Xiao, G., Wang, X., Zhang, X., & Friml, J. (2019). Evolution
of fast root gravitropism in seed plants. Nature Communications. Springer
Nature. https://doi.org/10.1038/s41467-019-11471-8
chicago: Zhang, Yuzhou, G Xiao, X Wang, Xixi Zhang, and Jiří Friml. “Evolution of
Fast Root Gravitropism in Seed Plants.” Nature Communications. Springer
Nature, 2019. https://doi.org/10.1038/s41467-019-11471-8.
ieee: Y. Zhang, G. Xiao, X. Wang, X. Zhang, and J. Friml, “Evolution of fast root
gravitropism in seed plants,” Nature Communications, vol. 10. Springer
Nature, 2019.
ista: Zhang Y, Xiao G, Wang X, Zhang X, Friml J. 2019. Evolution of fast root gravitropism
in seed plants. Nature Communications. 10, 3480.
mla: Zhang, Yuzhou, et al. “Evolution of Fast Root Gravitropism in Seed Plants.”
Nature Communications, vol. 10, 3480, Springer Nature, 2019, doi:10.1038/s41467-019-11471-8.
short: Y. Zhang, G. Xiao, X. Wang, X. Zhang, J. Friml, Nature Communications 10
(2019).
date_created: 2019-08-09T08:46:26Z
date_published: 2019-08-02T00:00:00Z
date_updated: 2023-08-29T07:02:44Z
day: '02'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41467-019-11471-8
ec_funded: 1
external_id:
isi:
- '000478576500012'
pmid:
- '31375675'
file:
- access_level: open_access
checksum: d2c654fdb97f33078f606fe0c298bf6e
content_type: application/pdf
creator: dernst
date_created: 2019-08-12T07:09:20Z
date_updated: 2020-07-14T12:47:40Z
file_id: '6798'
file_name: 2019_NatureComm_Zhang.pdf
file_size: 6406141
relation: main_file
file_date_updated: 2020-07-14T12:47:40Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/when-plant-roots-learned-to-follow-gravity/
scopus_import: '1'
status: public
title: Evolution of fast root gravitropism in seed plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2019'
...
---
_id: '6366'
abstract:
- lang: eng
text: Plants have a remarkable capacity to adjust their growth and development to
elevated ambient temperatures. Increased elongation growth of roots, hypocotyls
and petioles in warm temperatures are hallmarks of seedling thermomorphogenesis.
In the last decade, significant progress has been made to identify the molecular
signaling components regulating these growth responses. Increased ambient temperature
utilizes diverse components of the light sensing and signal transduction network
to trigger growth adjustments. However, it remains unknown whether temperature
sensing and responses are universal processes that occur uniformly in all plant
organs. Alternatively, temperature sensing may be confined to specific tissues
or organs, which would require a systemic signal that mediates responses in distal
parts of the plant. Here we show that Arabidopsis (Arabidopsis thaliana) seedlings
show organ-specific transcriptome responses to elevated temperatures, and that
thermomorphogenesis involves both autonomous and organ-interdependent temperature
sensing and signaling. Seedling roots can sense and respond to temperature in
a shoot-independent manner, whereas shoot temperature responses require both local
and systemic processes. The induction of cell elongation in hypocotyls requires
temperature sensing in cotyledons, followed by generation of a mobile auxin signal.
Subsequently, auxin travels to the hypocotyl where it triggers local brassinosteroid-induced
cell elongation in seedling stems, which depends upon a distinct, permissive temperature
sensor in the hypocotyl.
article_processing_charge: No
article_type: original
author:
- first_name: Julia
full_name: Bellstaedt, Julia
last_name: Bellstaedt
- first_name: Jana
full_name: Trenner, Jana
last_name: Trenner
- first_name: Rebecca
full_name: Lippmann, Rebecca
last_name: Lippmann
- first_name: Yvonne
full_name: Poeschl, Yvonne
last_name: Poeschl
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Marcel
full_name: Quint, Marcel
last_name: Quint
- first_name: Carolin
full_name: Delker, Carolin
last_name: Delker
citation:
ama: Bellstaedt J, Trenner J, Lippmann R, et al. A mobile auxin signal connects
temperature sensing in cotyledons with growth responses in hypocotyls. Plant
Physiology. 2019;180(2):757-766. doi:10.1104/pp.18.01377
apa: Bellstaedt, J., Trenner, J., Lippmann, R., Poeschl, Y., Zhang, X., Friml, J.,
… Delker, C. (2019). A mobile auxin signal connects temperature sensing in cotyledons
with growth responses in hypocotyls. Plant Physiology. ASPB. https://doi.org/10.1104/pp.18.01377
chicago: Bellstaedt, Julia, Jana Trenner, Rebecca Lippmann, Yvonne Poeschl, Xixi
Zhang, Jiří Friml, Marcel Quint, and Carolin Delker. “A Mobile Auxin Signal Connects
Temperature Sensing in Cotyledons with Growth Responses in Hypocotyls.” Plant
Physiology. ASPB, 2019. https://doi.org/10.1104/pp.18.01377.
ieee: J. Bellstaedt et al., “A mobile auxin signal connects temperature sensing
in cotyledons with growth responses in hypocotyls,” Plant Physiology, vol.
180, no. 2. ASPB, pp. 757–766, 2019.
ista: Bellstaedt J, Trenner J, Lippmann R, Poeschl Y, Zhang X, Friml J, Quint M,
Delker C. 2019. A mobile auxin signal connects temperature sensing in cotyledons
with growth responses in hypocotyls. Plant Physiology. 180(2), 757–766.
mla: Bellstaedt, Julia, et al. “A Mobile Auxin Signal Connects Temperature Sensing
in Cotyledons with Growth Responses in Hypocotyls.” Plant Physiology, vol.
180, no. 2, ASPB, 2019, pp. 757–66, doi:10.1104/pp.18.01377.
short: J. Bellstaedt, J. Trenner, R. Lippmann, Y. Poeschl, X. Zhang, J. Friml, M.
Quint, C. Delker, Plant Physiology 180 (2019) 757–766.
date_created: 2019-04-30T15:24:22Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2023-09-05T12:25:19Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.18.01377
external_id:
isi:
- '000470086100019'
pmid:
- '31000634'
intvolume: ' 180'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: www.doi.org/10.1104/pp.18.01377
month: '06'
oa: 1
oa_version: Published Version
page: 757-766
pmid: 1
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: ASPB
quality_controlled: '1'
scopus_import: '1'
status: public
title: A mobile auxin signal connects temperature sensing in cotyledons with growth
responses in hypocotyls
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 180
year: '2019'
...
---
_id: '6259'
abstract:
- lang: eng
text: The plant hormone auxin has crucial roles in almost all aspects of plant growth
and development. Concentrations of auxin vary across different tissues, mediating
distinct developmental outcomes and contributing to the functional diversity of
auxin. However, the mechanisms that underlie these activities are poorly understood.
Here we identify an auxin signalling mechanism, which acts in parallel to the
canonical auxin pathway based on the transport inhibitor response1 (TIR1) and
other auxin receptor F-box (AFB) family proteins (TIR1/AFB receptors)1,2, that
translates levels of cellular auxin to mediate differential growth during apical-hook
development. This signalling mechanism operates at the concave side of the apical
hook, and involves auxin-mediated C-terminal cleavage of transmembrane kinase
1 (TMK1). The cytosolic and nucleus-translocated C terminus of TMK1 specifically
interacts with and phosphorylates two non-canonical transcriptional repressors
of the auxin or indole-3-acetic acid (Aux/IAA) family (IAA32 and IAA34), thereby
regulating ARF transcription factors. In contrast to the degradation of Aux/IAA
transcriptional repressors in the canonical pathway, the newly identified mechanism
stabilizes the non-canonical IAA32 and IAA34 transcriptional repressors to regulate
gene expression and ultimately inhibit growth. The auxin–TMK1 signalling pathway
originates at the cell surface, is triggered by high levels of auxin and shares
a partially overlapping set of transcription factors with the TIR1/AFB signalling
pathway. This allows distinct interpretations of different concentrations of cellular
auxin, and thus enables this versatile signalling molecule to mediate complex
developmental outcomes.
article_processing_charge: No
article_type: original
author:
- first_name: Min
full_name: Cao, Min
last_name: Cao
- first_name: Rong
full_name: Chen, Rong
last_name: Chen
- first_name: Pan
full_name: Li, Pan
last_name: Li
- first_name: Yongqiang
full_name: Yu, Yongqiang
last_name: Yu
- first_name: Rui
full_name: Zheng, Rui
last_name: Zheng
- first_name: Danfeng
full_name: Ge, Danfeng
last_name: Ge
- first_name: Wei
full_name: Zheng, Wei
last_name: Zheng
- first_name: Xuhui
full_name: Wang, Xuhui
last_name: Wang
- first_name: Yangtao
full_name: Gu, Yangtao
last_name: Gu
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Heng
full_name: Zhang, Heng
last_name: Zhang
- first_name: Renyi
full_name: Liu, Renyi
last_name: Liu
- first_name: Jun
full_name: He, Jun
last_name: He
- first_name: Tongda
full_name: Xu, Tongda
last_name: Xu
citation:
ama: Cao M, Chen R, Li P, et al. TMK1-mediated auxin signalling regulates differential
growth of the apical hook. Nature. 2019;568:240-243. doi:10.1038/s41586-019-1069-7
apa: Cao, M., Chen, R., Li, P., Yu, Y., Zheng, R., Ge, D., … Xu, T. (2019). TMK1-mediated
auxin signalling regulates differential growth of the apical hook. Nature.
Springer Nature. https://doi.org/10.1038/s41586-019-1069-7
chicago: Cao, Min, Rong Chen, Pan Li, Yongqiang Yu, Rui Zheng, Danfeng Ge, Wei Zheng,
et al. “TMK1-Mediated Auxin Signalling Regulates Differential Growth of the Apical
Hook.” Nature. Springer Nature, 2019. https://doi.org/10.1038/s41586-019-1069-7.
ieee: M. Cao et al., “TMK1-mediated auxin signalling regulates differential
growth of the apical hook,” Nature, vol. 568. Springer Nature, pp. 240–243,
2019.
ista: Cao M, Chen R, Li P, Yu Y, Zheng R, Ge D, Zheng W, Wang X, Gu Y, Gelová Z,
Friml J, Zhang H, Liu R, He J, Xu T. 2019. TMK1-mediated auxin signalling regulates
differential growth of the apical hook. Nature. 568, 240–243.
mla: Cao, Min, et al. “TMK1-Mediated Auxin Signalling Regulates Differential Growth
of the Apical Hook.” Nature, vol. 568, Springer Nature, 2019, pp. 240–43,
doi:10.1038/s41586-019-1069-7.
short: M. Cao, R. Chen, P. Li, Y. Yu, R. Zheng, D. Ge, W. Zheng, X. Wang, Y. Gu,
Z. Gelová, J. Friml, H. Zhang, R. Liu, J. He, T. Xu, Nature 568 (2019) 240–243.
date_created: 2019-04-09T08:37:05Z
date_published: 2019-04-11T00:00:00Z
date_updated: 2023-09-05T14:58:41Z
day: '11'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41586-019-1069-7
ec_funded: 1
external_id:
isi:
- '000464412700050'
pmid:
- '30944466'
file:
- access_level: open_access
checksum: 6b84ab602a34382cf0340a37a1378c75
content_type: application/pdf
creator: dernst
date_created: 2020-11-13T07:37:41Z
date_updated: 2020-11-13T07:37:41Z
file_id: '8751'
file_name: 2019_Nature _Cao_accepted.pdf
file_size: 4321328
relation: main_file
success: 1
file_date_updated: 2020-11-13T07:37:41Z
has_accepted_license: '1'
intvolume: ' 568'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Submitted Version
page: 240-243
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature
publication_identifier:
eissn:
- 1476-4687
issn:
- 0028-0836
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/newly-discovered-mechanism-of-plant-hormone-auxin-acts-the-opposite-way/
scopus_import: '1'
status: public
title: TMK1-mediated auxin signalling regulates differential growth of the apical
hook
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 568
year: '2019'
...
---
_id: '7106'
abstract:
- lang: eng
text: PIN-FORMED (PIN) transporters mediate directional, intercellular movement
of the phytohormone auxin in land plants. To elucidate the evolutionary origins
of this developmentally crucial mechanism, we analysed the single PIN homologue
of a simple green alga Klebsormidium flaccidum. KfPIN functions as a plasma membrane-localized
auxin exporter in land plants and heterologous models. While its role in algae
remains unclear, PIN-driven auxin export is probably an ancient and conserved
trait within streptophytes.
article_processing_charge: No
article_type: original
author:
- first_name: Roman
full_name: Skokan, Roman
last_name: Skokan
- first_name: Eva
full_name: Medvecká, Eva
last_name: Medvecká
- first_name: Tom
full_name: Viaene, Tom
last_name: Viaene
- first_name: Stanislav
full_name: Vosolsobě, Stanislav
last_name: Vosolsobě
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Karel
full_name: Müller, Karel
last_name: Müller
- first_name: Petr
full_name: Skůpa, Petr
last_name: Skůpa
- first_name: Michal
full_name: Karady, Michal
last_name: Karady
- first_name: Yuzhou
full_name: Zhang, Yuzhou
last_name: Zhang
- first_name: Dorina P.
full_name: Janacek, Dorina P.
last_name: Janacek
- first_name: Ulrich Z.
full_name: Hammes, Ulrich Z.
last_name: Hammes
- first_name: Karin
full_name: Ljung, Karin
last_name: Ljung
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Skokan R, Medvecká E, Viaene T, et al. PIN-driven auxin transport emerged early
in streptophyte evolution. Nature Plants. 2019;5(11):1114-1119. doi:10.1038/s41477-019-0542-5
apa: Skokan, R., Medvecká, E., Viaene, T., Vosolsobě, S., Zwiewka, M., Müller, K.,
… Friml, J. (2019). PIN-driven auxin transport emerged early in streptophyte evolution.
Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-019-0542-5
chicago: Skokan, Roman, Eva Medvecká, Tom Viaene, Stanislav Vosolsobě, Marta Zwiewka,
Karel Müller, Petr Skůpa, et al. “PIN-Driven Auxin Transport Emerged Early in
Streptophyte Evolution.” Nature Plants. Springer Nature, 2019. https://doi.org/10.1038/s41477-019-0542-5.
ieee: R. Skokan et al., “PIN-driven auxin transport emerged early in streptophyte
evolution,” Nature Plants, vol. 5, no. 11. Springer Nature, pp. 1114–1119,
2019.
ista: Skokan R, Medvecká E, Viaene T, Vosolsobě S, Zwiewka M, Müller K, Skůpa P,
Karady M, Zhang Y, Janacek DP, Hammes UZ, Ljung K, Nodzyński T, Petrášek J, Friml
J. 2019. PIN-driven auxin transport emerged early in streptophyte evolution. Nature
Plants. 5(11), 1114–1119.
mla: Skokan, Roman, et al. “PIN-Driven Auxin Transport Emerged Early in Streptophyte
Evolution.” Nature Plants, vol. 5, no. 11, Springer Nature, 2019, pp. 1114–19,
doi:10.1038/s41477-019-0542-5.
short: R. Skokan, E. Medvecká, T. Viaene, S. Vosolsobě, M. Zwiewka, K. Müller, P.
Skůpa, M. Karady, Y. Zhang, D.P. Janacek, U.Z. Hammes, K. Ljung, T. Nodzyński,
J. Petrášek, J. Friml, Nature Plants 5 (2019) 1114–1119.
date_created: 2019-11-25T09:08:04Z
date_published: 2019-11-01T00:00:00Z
date_updated: 2023-09-06T11:09:49Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-019-0542-5
ec_funded: 1
external_id:
isi:
- '000496526100010'
pmid:
- '31712756'
file:
- access_level: open_access
checksum: 94e0426856aad9a9bd0135d5436efbf1
content_type: application/pdf
creator: dernst
date_created: 2020-10-14T08:54:49Z
date_updated: 2020-10-14T08:54:49Z
file_id: '8660'
file_name: 2019_NaturePlants_Skokan_accepted.pdf
file_size: 1980851
relation: main_file
success: 1
file_date_updated: 2020-10-14T08:54:49Z
has_accepted_license: '1'
intvolume: ' 5'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Submitted Version
page: 1114-1119
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: PIN-driven auxin transport emerged early in streptophyte evolution
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 5
year: '2019'
...
---
_id: '7143'
abstract:
- lang: eng
text: Roots grow downwards parallel to the gravity vector, to anchor a plant in
soil and acquire water and nutrients, using a gravitropic mechanism dependent
on the asymmetric distribution of the phytohormone auxin. Recently, Chang et al.
demonstrate that asymmetric distribution of another phytohormone, cytokinin, directs
root growth towards higher water content.
article_processing_charge: No
article_type: original
author:
- first_name: Scott A
full_name: Sinclair, Scott A
id: 2D99FE6A-F248-11E8-B48F-1D18A9856A87
last_name: Sinclair
orcid: 0000-0002-4566-0593
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Sinclair SA, Friml J. Defying gravity: a plant’s quest for moisture. Cell
Research. 2019;29:965-966. doi:10.1038/s41422-019-0254-4'
apa: 'Sinclair, S. A., & Friml, J. (2019). Defying gravity: a plant’s quest
for moisture. Cell Research. Springer Nature. https://doi.org/10.1038/s41422-019-0254-4'
chicago: 'Sinclair, Scott A, and Jiří Friml. “Defying Gravity: A Plant’s Quest for
Moisture.” Cell Research. Springer Nature, 2019. https://doi.org/10.1038/s41422-019-0254-4.'
ieee: 'S. A. Sinclair and J. Friml, “Defying gravity: a plant’s quest for moisture,”
Cell Research, vol. 29. Springer Nature, pp. 965–966, 2019.'
ista: 'Sinclair SA, Friml J. 2019. Defying gravity: a plant’s quest for moisture.
Cell Research. 29, 965–966.'
mla: 'Sinclair, Scott A., and Jiří Friml. “Defying Gravity: A Plant’s Quest for
Moisture.” Cell Research, vol. 29, Springer Nature, 2019, pp. 965–66, doi:10.1038/s41422-019-0254-4.'
short: S.A. Sinclair, J. Friml, Cell Research 29 (2019) 965–966.
date_created: 2019-12-02T12:30:48Z
date_published: 2019-12-01T00:00:00Z
date_updated: 2023-09-06T11:20:58Z
day: '01'
department:
- _id: JiFr
doi: 10.1038/s41422-019-0254-4
external_id:
isi:
- '000500749600001'
pmid:
- '31745287'
intvolume: ' 29'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1038/s41422-019-0254-4
month: '12'
oa: 1
oa_version: Published Version
page: 965-966
pmid: 1
publication: Cell Research
publication_identifier:
eissn:
- 1748-7838
issn:
- 1001-0602
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Defying gravity: a plant''s quest for moisture'
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 29
year: '2019'
...
---
_id: '7182'
abstract:
- lang: eng
text: During infection pathogens secrete small molecules, termed effectors, to manipulate
and control the interaction with their specific hosts. Both the pathogen and the
plant are under high selective pressure to rapidly adapt and co-evolve in what
is usually referred to as molecular arms race. Components of the host’s immune
system form a network that processes information about molecules with a foreign
origin and damage-associated signals, integrating them with developmental and
abiotic cues to adapt the plant’s responses. Both in the case of nucleotide-binding
leucine-rich repeat receptors and leucine-rich repeat receptor kinases interaction
networks have been extensively characterized. However, little is known on whether
pathogenic effectors form complexes to overcome plant immunity and promote disease.
Ustilago maydis, a biotrophic fungal pathogen that infects maize plants, produces
effectors that target hubs in the immune network of the host cell. Here we assess
the capability of U. maydis effector candidates to interact with each other, which
may play a crucial role during the infection process. Using a systematic yeast-two-hybrid
approach and based on a preliminary pooled screen, we selected 63 putative effectors
for one-on-one matings with a library of nearly 300 effector candidates. We found
that 126 of these effector candidates interacted either with themselves or other
predicted effectors. Although the functional relevance of the observed interactions
remains elusive, we propose that the observed abundance in complex formation between
effectors adds an additional level of complexity to effector research and should
be taken into consideration when studying effector evolution and function. Based
on this fundamental finding, we suggest various scenarios which could evolutionarily
drive the formation and stabilization of an effector interactome.
article_number: '1437'
article_processing_charge: No
article_type: original
author:
- first_name: André
full_name: Alcântara, André
last_name: Alcântara
- first_name: Jason
full_name: Bosch, Jason
last_name: Bosch
- first_name: Fahimeh
full_name: Nazari, Fahimeh
last_name: Nazari
- first_name: Gesa
full_name: Hoffmann, Gesa
last_name: Hoffmann
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Simon
full_name: Uhse, Simon
last_name: Uhse
- first_name: Martin A.
full_name: Darino, Martin A.
last_name: Darino
- first_name: Toluwase
full_name: Olukayode, Toluwase
last_name: Olukayode
- first_name: Daniel
full_name: Reumann, Daniel
last_name: Reumann
- first_name: Laura
full_name: Baggaley, Laura
last_name: Baggaley
- first_name: Armin
full_name: Djamei, Armin
last_name: Djamei
citation:
ama: Alcântara A, Bosch J, Nazari F, et al. Systematic Y2H screening reveals extensive
effector-complex formation. Frontiers in Plant Science. 2019;10(11). doi:10.3389/fpls.2019.01437
apa: Alcântara, A., Bosch, J., Nazari, F., Hoffmann, G., Gallei, M. C., Uhse, S.,
… Djamei, A. (2019). Systematic Y2H screening reveals extensive effector-complex
formation. Frontiers in Plant Science. Frontiers. https://doi.org/10.3389/fpls.2019.01437
chicago: Alcântara, André, Jason Bosch, Fahimeh Nazari, Gesa Hoffmann, Michelle
C Gallei, Simon Uhse, Martin A. Darino, et al. “Systematic Y2H Screening Reveals
Extensive Effector-Complex Formation.” Frontiers in Plant Science. Frontiers,
2019. https://doi.org/10.3389/fpls.2019.01437.
ieee: A. Alcântara et al., “Systematic Y2H screening reveals extensive effector-complex
formation,” Frontiers in Plant Science, vol. 10, no. 11. Frontiers, 2019.
ista: Alcântara A, Bosch J, Nazari F, Hoffmann G, Gallei MC, Uhse S, Darino MA,
Olukayode T, Reumann D, Baggaley L, Djamei A. 2019. Systematic Y2H screening reveals
extensive effector-complex formation. Frontiers in Plant Science. 10(11), 1437.
mla: Alcântara, André, et al. “Systematic Y2H Screening Reveals Extensive Effector-Complex
Formation.” Frontiers in Plant Science, vol. 10, no. 11, 1437, Frontiers,
2019, doi:10.3389/fpls.2019.01437.
short: A. Alcântara, J. Bosch, F. Nazari, G. Hoffmann, M.C. Gallei, S. Uhse, M.A.
Darino, T. Olukayode, D. Reumann, L. Baggaley, A. Djamei, Frontiers in Plant Science
10 (2019).
date_created: 2019-12-15T23:00:43Z
date_published: 2019-11-14T00:00:00Z
date_updated: 2023-09-06T14:33:46Z
day: '14'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3389/fpls.2019.01437
external_id:
isi:
- '000499821700001'
pmid:
- '31803201'
file:
- access_level: open_access
checksum: 995aa838aec2064d93550de82b40bbd1
content_type: application/pdf
creator: dernst
date_created: 2019-12-16T07:58:43Z
date_updated: 2020-07-14T12:47:52Z
file_id: '7185'
file_name: 2019_FrontiersPlant_Alcantara.pdf
file_size: 1532505
relation: main_file
file_date_updated: 2020-07-14T12:47:52Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: Systematic Y2H screening reveals extensive effector-complex formation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 10
year: '2019'
...
---
_id: '6377'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) is a highly conserved and essential cellular
process in eukaryotic cells, but its dynamic and vital nature makes it challenging
to study using classical genetics tools. In contrast, although small molecules
can acutely and reversibly perturb CME, the few chemical CME inhibitors that have
been applied to plants are either ineffective or show undesirable side effects.
Here, we identify the previously described endosidin9 (ES9) as an inhibitor of
clathrin heavy chain (CHC) function in both Arabidopsis and human cells through
affinity-based target isolation, in vitro binding studies and X-ray crystallography.
Moreover, we present a chemically improved ES9 analog, ES9-17, which lacks the
undesirable side effects of ES9 while retaining the ability to target CHC. ES9
and ES9-17 have expanded the chemical toolbox used to probe CHC function, and
present chemical scaffolds for further design of more specific and potent CHC
inhibitors across different systems.
article_processing_charge: No
article_type: original
author:
- first_name: Wim
full_name: Dejonghe, Wim
last_name: Dejonghe
- first_name: Isha
full_name: Sharma, Isha
last_name: Sharma
- first_name: Bram
full_name: Denoo, Bram
last_name: Denoo
- first_name: Steven
full_name: De Munck, Steven
last_name: De Munck
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Kiril
full_name: Mishev, Kiril
last_name: Mishev
- first_name: Haydar
full_name: Bulut, Haydar
last_name: Bulut
- first_name: Evelien
full_name: Mylle, Evelien
last_name: Mylle
- first_name: Riet
full_name: De Rycke, Riet
last_name: De Rycke
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
- first_name: Daniel V.
full_name: Savatin, Daniel V.
last_name: Savatin
- first_name: Wim
full_name: Nerinckx, Wim
last_name: Nerinckx
- first_name: An
full_name: Staes, An
last_name: Staes
- first_name: Andrzej
full_name: Drozdzecki, Andrzej
last_name: Drozdzecki
- first_name: Dominique
full_name: Audenaert, Dominique
last_name: Audenaert
- first_name: Klaas
full_name: Yperman, Klaas
last_name: Yperman
- first_name: Annemieke
full_name: Madder, Annemieke
last_name: Madder
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Daniël
full_name: Van Damme, Daniël
last_name: Van Damme
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Volker
full_name: Haucke, Volker
last_name: Haucke
- first_name: Savvas N.
full_name: Savvides, Savvas N.
last_name: Savvides
- first_name: Johan
full_name: Winne, Johan
last_name: Winne
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Dejonghe W, Sharma I, Denoo B, et al. Disruption of endocytosis through chemical
inhibition of clathrin heavy chain function. Nature Chemical Biology. 2019;15(6):641–649.
doi:10.1038/s41589-019-0262-1
apa: Dejonghe, W., Sharma, I., Denoo, B., De Munck, S., Lu, Q., Mishev, K., … Russinova,
E. (2019). Disruption of endocytosis through chemical inhibition of clathrin heavy
chain function. Nature Chemical Biology. Springer Nature. https://doi.org/10.1038/s41589-019-0262-1
chicago: Dejonghe, Wim, Isha Sharma, Bram Denoo, Steven De Munck, Qing Lu, Kiril
Mishev, Haydar Bulut, et al. “Disruption of Endocytosis through Chemical Inhibition
of Clathrin Heavy Chain Function.” Nature Chemical Biology. Springer Nature,
2019. https://doi.org/10.1038/s41589-019-0262-1.
ieee: W. Dejonghe et al., “Disruption of endocytosis through chemical inhibition
of clathrin heavy chain function,” Nature Chemical Biology, vol. 15, no.
6. Springer Nature, pp. 641–649, 2019.
ista: Dejonghe W, Sharma I, Denoo B, De Munck S, Lu Q, Mishev K, Bulut H, Mylle
E, De Rycke R, Vasileva MK, Savatin DV, Nerinckx W, Staes A, Drozdzecki A, Audenaert
D, Yperman K, Madder A, Friml J, Van Damme D, Gevaert K, Haucke V, Savvides SN,
Winne J, Russinova E. 2019. Disruption of endocytosis through chemical inhibition
of clathrin heavy chain function. Nature Chemical Biology. 15(6), 641–649.
mla: Dejonghe, Wim, et al. “Disruption of Endocytosis through Chemical Inhibition
of Clathrin Heavy Chain Function.” Nature Chemical Biology, vol. 15, no.
6, Springer Nature, 2019, pp. 641–649, doi:10.1038/s41589-019-0262-1.
short: W. Dejonghe, I. Sharma, B. Denoo, S. De Munck, Q. Lu, K. Mishev, H. Bulut,
E. Mylle, R. De Rycke, M.K. Vasileva, D.V. Savatin, W. Nerinckx, A. Staes, A.
Drozdzecki, D. Audenaert, K. Yperman, A. Madder, J. Friml, D. Van Damme, K. Gevaert,
V. Haucke, S.N. Savvides, J. Winne, E. Russinova, Nature Chemical Biology 15 (2019)
641–649.
date_created: 2019-05-05T21:59:11Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2023-09-07T12:54:35Z
day: '01'
department:
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publisher: Springer Nature
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relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Disruption of endocytosis through chemical inhibition of clathrin heavy chain
function
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 15
year: '2019'
...
---
_id: '7172'
abstract:
- lang: eng
text: "The development and growth of Arabidopsis thaliana is regulated by a combination
of genetic programing and also by the environmental influences. An important role
in these processes play the phytohormones and among them, auxin is crucial as
it controls many important functions. It is transported through the whole plant
body by creating local and temporal concentration maxima and minima, which have
an impact on the cell status, tissue and organ identity. Auxin has the property
to undergo a directional and finely regulated cell-to-cell transport, which is
enabled by the transport proteins, localized on the plasma membrane. An important
role in this process have the PIN auxin efflux proteins, which have an asymmetric/polar
subcellular localization and determine the directionality of the auxin transport.
During the last years, there were significant advances in understanding how the
trafficking molecular machineries function, including studies on molecular interactions,
function, subcellular localization and intracellular distribution. However, there
is still a lack of detailed characterization on the steps of endocytosis, exocytosis,
endocytic recycling and degradation. Due to this fact, I focused on the identification
of novel trafficking factors and better characterization of the intracellular
trafficking pathways. My PhD thesis consists of an introductory chapter, three
experimental chapters, a chapter containing general discussion, conclusions and
perspectives and also an appendix chapter with published collaborative papers.\r\nThe
first chapter is separated in two different parts: I start by a general introduction
to auxin biology and then I introduce the trafficking pathways in the model plant
Arabidopsis thaliana. Then, I explain also the phosphorylation-signals for polar
targeting and also the roles of the phytohormone strigolactone.\r\nThe second
chapter includes the characterization of bar1/sacsin mutant, which was identified
in a forward genetic screen for novel trafficking components in Arabidopsis thaliana,
where by the implementation of an EMS-treated pPIN1::PIN1-GFP marker line and
by using the established inhibitor of ARF-GEFs, Brefeldin A (BFA) as a tool to
study trafficking processes, we identified a novel factor, which is mediating
the adaptation of the plant cell to ARF-GEF inhibition. The mutation is in a previously
uncharacterized gene, encoding a very big protein that we, based on its homologies,
called SACSIN with domains suggesting roles as a molecular chaperon or as a component
of the ubiquitin-proteasome system. Our physiology and imaging studies revealed
that SACSIN is a crucial plant cell component of the adaptation to the ARF-GEF
inhibition.\r\nThe third chapter includes six subchapters, where I focus on the
role of the phytohormone strigolactone, which interferes with auxin feedback on
PIN internalization. Strigolactone moderates the polar auxin transport by increasing
the internalization of the PIN auxin efflux carriers, which reduces the canalization
related growth responses. In addition, I also studied the role of phosphorylation
in the strigolactone regulation of auxin feedback on PIN internalization. In this
chapter I also present my results on the MAX2-dependence of strigolactone-mediated
root growth inhibition and I also share my results on the auxin metabolomics profiling
after application of GR24.\r\nIn the fourth chapter I studied the effect of two
small molecules ES-9 and ES9-17, which were identified from a collection of small
molecules with the property to impair the clathrin-mediated endocytosis.\r\nIn
the fifth chapter, I discuss all my observations and experimental findings and
suggest alternative hypothesis to interpret my results.\r\nIn the appendix there
are three collaborative published projects. In the first, I participated in the
characterization of the role of ES9 as a small molecule, which is inhibitor of
clathrin- mediated endocytosis in different model organisms. In the second paper,
I contributed to the characterization of another small molecule ES9-17, which
is a non-protonophoric analog of ES9 and also impairs the clathrin-mediated endocytosis
not only in plant cells, but also in mammalian HeLa cells. Last but not least,
I also attach another paper, where I tried to establish the grafting method as
a technique in our lab to study canalization related processes."
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
citation:
ama: Vasileva MK. Molecular mechanisms of endomembrane trafficking in Arabidopsis
thaliana. 2019. doi:10.15479/AT:ISTA:7172
apa: Vasileva, M. K. (2019). Molecular mechanisms of endomembrane trafficking
in Arabidopsis thaliana. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:7172
chicago: Vasileva, Mina K. “Molecular Mechanisms of Endomembrane Trafficking in
Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2019. https://doi.org/10.15479/AT:ISTA:7172.
ieee: M. K. Vasileva, “Molecular mechanisms of endomembrane trafficking in Arabidopsis
thaliana,” Institute of Science and Technology Austria, 2019.
ista: Vasileva MK. 2019. Molecular mechanisms of endomembrane trafficking in Arabidopsis
thaliana. Institute of Science and Technology Austria.
mla: Vasileva, Mina K. Molecular Mechanisms of Endomembrane Trafficking in Arabidopsis
Thaliana. Institute of Science and Technology Austria, 2019, doi:10.15479/AT:ISTA:7172.
short: M.K. Vasileva, Molecular Mechanisms of Endomembrane Trafficking in Arabidopsis
Thaliana, Institute of Science and Technology Austria, 2019.
date_created: 2019-12-11T21:24:39Z
date_published: 2019-12-12T00:00:00Z
date_updated: 2023-09-19T10:39:33Z
day: '12'
ddc:
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page: '192'
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status: public
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status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: Molecular mechanisms of endomembrane trafficking in Arabidopsis thaliana
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2019'
...
---
_id: '6999'
abstract:
- lang: eng
text: Plasmodesmata (PD) are plant-specific membrane-lined channels that create
cytoplasmic and membrane continuities between adjacent cells, thereby facilitating
cell–cell communication and virus movement. Plant cells have evolved diverse mechanisms
to regulate PD plasticity in response to numerous environmental stimuli. In particular,
during defense against plant pathogens, the defense hormone, salicylic acid (SA),
plays a crucial role in the regulation of PD permeability in a callose-dependent
manner. Here, we uncover a mechanism by which plants restrict the spreading of
virus and PD cargoes using SA signaling by increasing lipid order and closure
of PD. We showed that exogenous SA application triggered the compartmentalization
of lipid raft nanodomains through a modulation of the lipid raft-regulatory protein,
Remorin (REM). Genetic studies, superresolution imaging, and transmission electron
microscopy observation together demonstrated that Arabidopsis REM1.2 and REM1.3
are crucial for plasma membrane nanodomain assembly to control PD aperture and
functionality. In addition, we also found that a 14-3-3 epsilon protein modulates
REM clustering and membrane nanodomain compartmentalization through its direct
interaction with REM proteins. This study unveils a molecular mechanism by which
the key plant defense hormone, SA, triggers membrane lipid nanodomain reorganization,
thereby regulating PD closure to impede virus spreading.
article_processing_charge: No
article_type: original
author:
- first_name: D
full_name: Huang, D
last_name: Huang
- first_name: Y
full_name: Sun, Y
last_name: Sun
- first_name: Z
full_name: Ma, Z
last_name: Ma
- first_name: M
full_name: Ke, M
last_name: Ke
- first_name: Y
full_name: Cui, Y
last_name: Cui
- first_name: Z
full_name: Chen, Z
last_name: Chen
- first_name: C
full_name: Chen, C
last_name: Chen
- first_name: C
full_name: Ji, C
last_name: Ji
- first_name: TM
full_name: Tran, TM
last_name: Tran
- first_name: L
full_name: Yang, L
last_name: Yang
- first_name: SM
full_name: Lam, SM
last_name: Lam
- first_name: Y
full_name: Han, Y
last_name: Han
- first_name: G
full_name: Shu, G
last_name: Shu
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Y
full_name: Miao, Y
last_name: Miao
- first_name: L
full_name: Jiang, L
last_name: Jiang
- first_name: X
full_name: Chen, X
last_name: Chen
citation:
ama: Huang D, Sun Y, Ma Z, et al. Salicylic acid-mediated plasmodesmal closure via
Remorin-dependent lipid organization. Proceedings of the National Academy of
Sciences of the United States of America. 2019;116(42):21274-21284. doi:10.1073/pnas.1911892116
apa: Huang, D., Sun, Y., Ma, Z., Ke, M., Cui, Y., Chen, Z., … Chen, X. (2019). Salicylic
acid-mediated plasmodesmal closure via Remorin-dependent lipid organization. Proceedings
of the National Academy of Sciences of the United States of America. Proceedings
of the National Academy of Sciences. https://doi.org/10.1073/pnas.1911892116
chicago: Huang, D, Y Sun, Z Ma, M Ke, Y Cui, Z Chen, C Chen, et al. “Salicylic Acid-Mediated
Plasmodesmal Closure via Remorin-Dependent Lipid Organization.” Proceedings
of the National Academy of Sciences of the United States of America. Proceedings
of the National Academy of Sciences, 2019. https://doi.org/10.1073/pnas.1911892116.
ieee: D. Huang et al., “Salicylic acid-mediated plasmodesmal closure via
Remorin-dependent lipid organization,” Proceedings of the National Academy
of Sciences of the United States of America, vol. 116, no. 42. Proceedings
of the National Academy of Sciences, pp. 21274–21284, 2019.
ista: Huang D, Sun Y, Ma Z, Ke M, Cui Y, Chen Z, Chen C, Ji C, Tran T, Yang L, Lam
S, Han Y, Shu G, Friml J, Miao Y, Jiang L, Chen X. 2019. Salicylic acid-mediated
plasmodesmal closure via Remorin-dependent lipid organization. Proceedings of
the National Academy of Sciences of the United States of America. 116(42), 21274–21284.
mla: Huang, D., et al. “Salicylic Acid-Mediated Plasmodesmal Closure via Remorin-Dependent
Lipid Organization.” Proceedings of the National Academy of Sciences of the
United States of America, vol. 116, no. 42, Proceedings of the National Academy
of Sciences, 2019, pp. 21274–84, doi:10.1073/pnas.1911892116.
short: D. Huang, Y. Sun, Z. Ma, M. Ke, Y. Cui, Z. Chen, C. Chen, C. Ji, T. Tran,
L. Yang, S. Lam, Y. Han, G. Shu, J. Friml, Y. Miao, L. Jiang, X. Chen, Proceedings
of the National Academy of Sciences of the United States of America 116 (2019)
21274–21284.
date_created: 2019-11-12T11:42:05Z
date_published: 2019-10-15T00:00:00Z
date_updated: 2023-10-17T12:32:37Z
day: '15'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.1911892116
external_id:
isi:
- '000490183000068'
pmid:
- '31575745'
file:
- access_level: open_access
checksum: 258c666bc6253eab81961f61169eefae
content_type: application/pdf
creator: dernst
date_created: 2019-11-13T08:22:28Z
date_updated: 2020-07-14T12:47:46Z
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file_name: 2019_PNAS_Huang.pdf
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language:
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month: '10'
oa: 1
oa_version: Published Version
page: 21274-21284
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: Proceedings of the National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1073/pnas.2004738117
scopus_import: '1'
status: public
title: Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
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(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 116
year: '2019'
...