[{"month":"07","publication_identifier":{"issn":["1745-2473"],"eissn":["1745-2481"]},"language":[{"iso":"eng"}],"doi":"10.1038/s41567-023-01977-w","quality_controlled":"1","isi":1,"project":[{"_id":"B6FC0238-B512-11E9-945C-1524E6697425","grant_number":"680037","name":"Coordination of Patterning And Growth In the Spinal Cord","call_identifier":"H2020"},{"_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","grant_number":"101044579","name":"Mechanisms of tissue size regulation in spinal cord development"},{"name":"Morphogen control of growth and pattern in the spinal cord","grant_number":"F07802","_id":"059DF620-7A3F-11EA-A408-12923DDC885E"},{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["000964029300003"]},"oa":1,"file_date_updated":"2023-10-04T11:13:28Z","ec_funded":1,"date_updated":"2023-10-04T11:14:05Z","date_created":"2023-04-16T22:01:09Z","volume":19,"author":[{"id":"4896F754-F248-11E8-B48F-1D18A9856A87","first_name":"Laura","last_name":"Bocanegra","full_name":"Bocanegra, Laura"},{"full_name":"Singh, Amrita","id":"76250f9f-3a21-11eb-9a80-a6180a0d7958","last_name":"Singh","first_name":"Amrita"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B"},{"full_name":"Zagórski, Marcin P","last_name":"Zagórski","first_name":"Marcin P","orcid":"0000-0001-7896-7762","id":"343DA0DC-F248-11E8-B48F-1D18A9856A87"},{"id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4509-4998","first_name":"Anna","last_name":"Kicheva","full_name":"Kicheva, Anna"}],"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"13081"}]},"publication_status":"published","department":[{"_id":"EdHa"},{"_id":"AnKi"}],"publisher":"Springer Nature","acknowledgement":"We thank S. Hippenmeyer for the reagents and C. P. Heisenberg, J. Briscoe and K. Page for comments on the manuscript. This work was supported by IST Austria; the European Research Council under Horizon 2020 research and innovation programme grant no. 680037 and Horizon Europe grant 101044579 (A.K.); Austrian Science Fund (FWF): F78 (Stem Cell Modulation) (A.K.); ISTFELLOW postdoctoral program (A.S.); Narodowe Centrum Nauki, Poland SONATA, 2017/26/D/NZ2/00454 (M.Z.); and the Polish National Agency for Academic Exchange (M.Z.).","year":"2023","day":"01","has_accepted_license":"1","article_processing_charge":"No","scopus_import":"1","date_published":"2023-07-01T00:00:00Z","article_type":"original","page":"1050-1058","publication":"Nature Physics","citation":{"chicago":"Bocanegra, Laura, Amrita Singh, Edouard B Hannezo, Marcin P Zagórski, and Anna Kicheva. “Cell Cycle Dynamics Control Fluidity of the Developing Mouse Neuroepithelium.” Nature Physics. Springer Nature, 2023. https://doi.org/10.1038/s41567-023-01977-w.","short":"L. Bocanegra, A. Singh, E.B. Hannezo, M.P. Zagórski, A. Kicheva, Nature Physics 19 (2023) 1050–1058.","mla":"Bocanegra, Laura, et al. “Cell Cycle Dynamics Control Fluidity of the Developing Mouse Neuroepithelium.” Nature Physics, vol. 19, Springer Nature, 2023, pp. 1050–58, doi:10.1038/s41567-023-01977-w.","ieee":"L. Bocanegra, A. Singh, E. B. Hannezo, M. P. Zagórski, and A. Kicheva, “Cell cycle dynamics control fluidity of the developing mouse neuroepithelium,” Nature Physics, vol. 19. Springer Nature, pp. 1050–1058, 2023.","apa":"Bocanegra, L., Singh, A., Hannezo, E. B., Zagórski, M. P., & Kicheva, A. (2023). Cell cycle dynamics control fluidity of the developing mouse neuroepithelium. Nature Physics. Springer Nature. https://doi.org/10.1038/s41567-023-01977-w","ista":"Bocanegra L, Singh A, Hannezo EB, Zagórski MP, Kicheva A. 2023. Cell cycle dynamics control fluidity of the developing mouse neuroepithelium. Nature Physics. 19, 1050–1058.","ama":"Bocanegra L, Singh A, Hannezo EB, Zagórski MP, Kicheva A. Cell cycle dynamics control fluidity of the developing mouse neuroepithelium. Nature Physics. 2023;19:1050-1058. doi:10.1038/s41567-023-01977-w"},"abstract":[{"text":"As developing tissues grow in size and undergo morphogenetic changes, their material properties may be altered. Such changes result from tension dynamics at cell contacts or cellular jamming. Yet, in many cases, the cellular mechanisms controlling the physical state of growing tissues are unclear. We found that at early developmental stages, the epithelium in the developing mouse spinal cord maintains both high junctional tension and high fluidity. This is achieved via a mechanism in which interkinetic nuclear movements generate cell area dynamics that drive extensive cell rearrangements. Over time, the cell proliferation rate declines, effectively solidifying the tissue. Thus, unlike well-studied jamming transitions, the solidification uncovered here resembles a glass transition that depends on the dynamical stresses generated by proliferation and differentiation. Our finding that the fluidity of developing epithelia is linked to interkinetic nuclear movements and the dynamics of growth is likely to be relevant to multiple developing tissues.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","file":[{"date_updated":"2023-10-04T11:13:28Z","date_created":"2023-10-04T11:13:28Z","checksum":"858225a4205b74406e5045006cdd853f","success":1,"relation":"main_file","file_id":"14392","file_size":5532285,"content_type":"application/pdf","creator":"dernst","file_name":"2023_NaturePhysics_Boncanegra.pdf","access_level":"open_access"}],"title":"Cell cycle dynamics control fluidity of the developing mouse neuroepithelium","status":"public","ddc":["570"],"intvolume":" 19","_id":"12837","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"date_published":"2023-05-23T00:00:00Z","citation":{"ieee":"L. Bocanegra, “Epithelial dynamics during mouse neural tube development,” Institute of Science and Technology Austria, 2023.","apa":"Bocanegra, L. (2023). Epithelial dynamics during mouse neural tube development. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:13081","ista":"Bocanegra L. 2023. Epithelial dynamics during mouse neural tube development. Institute of Science and Technology Austria.","ama":"Bocanegra L. Epithelial dynamics during mouse neural tube development. 2023. doi:10.15479/at:ista:13081","chicago":"Bocanegra, Laura. “Epithelial Dynamics during Mouse Neural Tube Development.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:13081.","short":"L. Bocanegra, Epithelial Dynamics during Mouse Neural Tube Development, Institute of Science and Technology Austria, 2023.","mla":"Bocanegra, Laura. Epithelial Dynamics during Mouse Neural Tube Development. Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:13081."},"page":"93","has_accepted_license":"1","article_processing_charge":"No","day":"23","oa_version":"Published Version","file":[{"date_updated":"2023-05-25T06:32:12Z","date_created":"2023-05-25T06:32:12Z","checksum":"74f3f89e59a0189bee53ebfad9c1b9af","relation":"source_file","file_id":"13089","file_size":25615534,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","creator":"lbocaneg","file_name":"Thesis_final_LauraBocanegra.docx","access_level":"closed"},{"embargo":"2024-05-31","file_id":"13090","relation":"main_file","date_updated":"2023-05-25T06:32:16Z","date_created":"2023-05-25T06:32:16Z","checksum":"c6cdef6323eacfb4b7a8af20f32eae97","file_name":"TotalFinal_Thesis_LauraBocanegraArx.pdf","embargo_to":"open_access","access_level":"closed","creator":"lbocaneg","content_type":"application/pdf","file_size":12386046}],"_id":"13081","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","status":"public","title":"Epithelial dynamics during mouse neural tube development","ddc":["570"],"abstract":[{"lang":"eng","text":"During development, tissues undergo changes in size and shape to form functional organs. Distinct cellular processes such as cell division and cell rearrangements underlie tissue morphogenesis. Yet how the distinct processes are controlled and coordinated, and how they contribute to morphogenesis is poorly understood. In our study, we addressed these questions using the developing mouse neural tube. This epithelial organ transforms from a flat epithelial sheet to an epithelial tube while increasing in size and undergoing morpho-gen-mediated patterning. The extent and mechanism of neural progenitor rearrangement within the developing mouse neuroepithelium is unknown. To investigate this, we per-formed high resolution lineage tracing analysis to quantify the extent of epithelial rear-rangement at different stages of neural tube development. We quantitatively described the relationship between apical cell size with cell cycle dependent interkinetic nuclear migra-tions (IKNM) and performed high cellular resolution live imaging of the neuroepithelium to study the dynamics of junctional remodeling. Furthermore, developed a vertex model of the neuroepithelium to investigate the quantitative contribution of cell proliferation, cell differentiation and mechanical properties to the epithelial rearrangement dynamics and validated the model predictions through functional experiments. Our analysis revealed that at early developmental stages, the apical cell area kinetics driven by IKNM induce high lev-els of cell rearrangements in a regime of high junctional tension and contractility. After E9.5, there is a sharp decline in the extent of cell rearrangements, suggesting that the epi-thelium transitions from a fluid-like to a solid-like state. We found that this transition is regulated by the growth rate of the tissue, rather than by changes in cell-cell adhesion and contractile forces. Overall, our study provides a quantitative description of the relationship between tissue growth, cell cycle dynamics, epithelia rearrangements and the emergent tissue material properties, and novel insights on how epithelial cell dynamics influences tissue morphogenesis."}],"type":"dissertation","alternative_title":["ISTA Thesis"],"doi":"10.15479/at:ista:13081","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"supervisor":[{"first_name":"Anna","last_name":"Kicheva","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4509-4998","full_name":"Kicheva, Anna"}],"degree_awarded":"PhD","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"publication_identifier":{"issn":["2663 - 337X"]},"month":"05","related_material":{"record":[{"relation":"part_of_dissertation","status":"public","id":"9349"},{"id":"12837","status":"public","relation":"part_of_dissertation"}]},"author":[{"full_name":"Bocanegra, Laura","first_name":"Laura","last_name":"Bocanegra","id":"4896F754-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2023-05-23T19:10:42Z","date_updated":"2023-10-04T11:14:04Z","year":"2023","publisher":"Institute of Science and Technology Austria","department":[{"_id":"GradSch"},{"_id":"AnKi"}],"publication_status":"published","file_date_updated":"2023-05-25T06:32:16Z"},{"doi":"10.1146/annurev-cellbio-020823-011522","language":[{"iso":"eng"}],"external_id":{"pmid":["37418774"]},"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"oa":1,"quality_controlled":"1","project":[{"name":"Coordination of Patterning And Growth In the Spinal Cord","call_identifier":"H2020","_id":"B6FC0238-B512-11E9-945C-1524E6697425","grant_number":"680037"},{"name":"Mechanisms of tissue size regulation in spinal cord development","_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","grant_number":"101044579"},{"name":"Morphogen control of growth and pattern in the spinal cord","_id":"059DF620-7A3F-11EA-A408-12923DDC885E","grant_number":"F07802"}],"month":"10","publication_identifier":{"eissn":["1530-8995"],"issn":["1081-0706"]},"author":[{"full_name":"Kicheva, Anna","last_name":"Kicheva","first_name":"Anna","orcid":"0000-0003-4509-4998","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Briscoe, James","last_name":"Briscoe","first_name":"James"}],"date_created":"2023-11-05T23:00:53Z","date_updated":"2023-11-06T09:56:24Z","volume":39,"year":"2023","acknowledgement":"We are grateful to Zena Hadjivasiliou for comments on this article. A.K. is supported by grants from the European Research Council under the European Union (EU) Horizon 2020 research and innovation program (680037) and Horizon Europe (101044579), and the Austrian Science Fund (F78) (Stem Cell Modulation). J.B. is supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK (CC001051), the UK Medical Research Council (CC001051), and the Wellcome Trust (CC001051), and by a grant from the European Research Council under the EU Horizon 2020 research and innovation program (742138).","pmid":1,"publication_status":"published","department":[{"_id":"AnKi"}],"publisher":"Annual Reviews","file_date_updated":"2023-11-06T09:47:50Z","ec_funded":1,"date_published":"2023-10-16T00:00:00Z","publication":"Annual Review of Cell and Developmental Biology","citation":{"short":"A. Kicheva, J. Briscoe, Annual Review of Cell and Developmental Biology 39 (2023) 91–121.","mla":"Kicheva, Anna, and James Briscoe. “Control of Tissue Development by Morphogens.” Annual Review of Cell and Developmental Biology, vol. 39, Annual Reviews, 2023, pp. 91–121, doi:10.1146/annurev-cellbio-020823-011522.","chicago":"Kicheva, Anna, and James Briscoe. “Control of Tissue Development by Morphogens.” Annual Review of Cell and Developmental Biology. Annual Reviews, 2023. https://doi.org/10.1146/annurev-cellbio-020823-011522.","ama":"Kicheva A, Briscoe J. Control of tissue development by morphogens. Annual Review of Cell and Developmental Biology. 2023;39:91-121. doi:10.1146/annurev-cellbio-020823-011522","ieee":"A. Kicheva and J. Briscoe, “Control of tissue development by morphogens,” Annual Review of Cell and Developmental Biology, vol. 39. Annual Reviews, pp. 91–121, 2023.","apa":"Kicheva, A., & Briscoe, J. (2023). Control of tissue development by morphogens. Annual Review of Cell and Developmental Biology. Annual Reviews. https://doi.org/10.1146/annurev-cellbio-020823-011522","ista":"Kicheva A, Briscoe J. 2023. Control of tissue development by morphogens. Annual Review of Cell and Developmental Biology. 39, 91–121."},"article_type":"review","page":"91-121","day":"16","article_processing_charge":"Yes (in subscription journal)","has_accepted_license":"1","scopus_import":"1","oa_version":"Published Version","file":[{"date_created":"2023-11-06T09:47:50Z","date_updated":"2023-11-06T09:47:50Z","checksum":"461726014cf5907010afbd418d3c13ec","success":1,"relation":"main_file","file_id":"14491","file_size":434819,"content_type":"application/pdf","creator":"dernst","file_name":"2023_AnnualReviews_Kicheva.pdf","access_level":"open_access"}],"_id":"14484","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Control of tissue development by morphogens","status":"public","ddc":["570"],"intvolume":" 39","abstract":[{"text":"Intercellular signaling molecules, known as morphogens, act at a long range in developing tissues to provide spatial information and control properties such as cell fate and tissue growth. The production, transport, and removal of morphogens shape their concentration profiles in time and space. Downstream signaling cascades and gene regulatory networks within cells then convert the spatiotemporal morphogen profiles into distinct cellular responses. Current challenges are to understand the diverse molecular and cellular mechanisms underlying morphogen gradient formation, as well as the logic of downstream regulatory circuits involved in morphogen interpretation. This knowledge, combining experimental and theoretical results, is essential to understand emerging properties of morphogen-controlled systems, such as robustness and scaling.","lang":"eng"}],"type":"journal_article"},{"article_number":"dev201559","file_date_updated":"2024-01-10T12:41:13Z","year":"2023","acknowledgement":"We thank members of the Brand lab, as well as Justina Stark (Ivo Sbalzarini group, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany) for project-related discussions; Darren Gilmour (University of Zurich), Karuna Sampath (University of Warwick) and Gokul Kesavan (Vowels Lifesciences Private Limited, Bangalore) for comments on the manuscript; personnel of the CMCB technology platform, TU Dresden for imaging and image analysis-related support; and Maurizio Abbate (Technical support, Arivis) for help with image analysis. We are also grateful to Stapornwongkul and Briscoe for commenting on a preprint version of our work (Stapornwongkul and Briscoe, 2022).\r\nThis work was supported by the Deutsche Forschungsgemeinschaft (BR 1746/6-2, BR 1746/11-1 and BR 1746/3 to M.B.), by a Cluster of Excellence ‘Physics of Life’ seed grant and by institutional funds from Technische Universitat Dresden (to M.B.). Open Access funding provided by Technische Universitat Dresden. Deposited in PMC for immediate release.","pmid":1,"publication_status":"published","publisher":"The Company of Biologists","department":[{"_id":"AnKi"}],"author":[{"id":"1bae78aa-ee0e-11ec-9b76-bc42990f409d","first_name":"Rohit K","last_name":"Harish","full_name":"Harish, Rohit K"},{"last_name":"Gupta","first_name":"Mansi","full_name":"Gupta, Mansi"},{"full_name":"Zöller, Daniela","last_name":"Zöller","first_name":"Daniela"},{"full_name":"Hartmann, Hella","first_name":"Hella","last_name":"Hartmann"},{"full_name":"Gheisari, Ali","first_name":"Ali","last_name":"Gheisari"},{"last_name":"Machate","first_name":"Anja","full_name":"Machate, Anja"},{"full_name":"Hans, Stefan","last_name":"Hans","first_name":"Stefan"},{"first_name":"Michael","last_name":"Brand","full_name":"Brand, Michael"}],"date_created":"2024-01-10T09:18:54Z","date_updated":"2024-01-10T12:45:25Z","volume":150,"month":"10","publication_identifier":{"issn":["0950-1991"],"eissn":["1477-9129"]},"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["001097449100002"],"pmid":["37665167"]},"isi":1,"quality_controlled":"1","doi":"10.1242/dev.201559","language":[{"iso":"eng"}],"type":"journal_article","abstract":[{"text":"Morphogen gradients impart positional information to cells in a homogenous tissue field. Fgf8a, a highly conserved growth factor, has been proposed to act as a morphogen during zebrafish gastrulation. However, technical limitations have so far prevented direct visualization of the endogenous Fgf8a gradient and confirmation of its morphogenic activity. Here, we monitor Fgf8a propagation in the developing neural plate using a CRISPR/Cas9-mediated EGFP knock-in at the endogenous fgf8a locus. By combining sensitive imaging with single-molecule fluorescence correlation spectroscopy, we demonstrate that Fgf8a, which is produced at the embryonic margin, propagates by diffusion through the extracellular space and forms a graded distribution towards the animal pole. Overlaying the Fgf8a gradient curve with expression profiles of its downstream targets determines the precise input-output relationship of Fgf8a-mediated patterning. Manipulation of the extracellular Fgf8a levels alters the signaling outcome, thus establishing Fgf8a as a bona fide morphogen during zebrafish gastrulation. Furthermore, by hindering Fgf8a diffusion, we demonstrate that extracellular diffusion of the protein from the source is crucial for it to achieve its morphogenic potential.","lang":"eng"}],"issue":"19","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"14774","status":"public","title":"Real-time monitoring of an endogenous Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation","ddc":["570"],"intvolume":" 150","oa_version":"Published Version","file":[{"creator":"dernst","content_type":"application/pdf","file_size":12836306,"file_name":"2023_Development_Harish.pdf","access_level":"open_access","date_updated":"2024-01-10T12:41:13Z","date_created":"2024-01-10T12:41:13Z","success":1,"checksum":"2d6f52dc33260a9b2352b8f28374ba5f","file_id":"14790","relation":"main_file"}],"keyword":["Developmental Biology","Molecular Biology"],"day":"01","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","publication":"Development","citation":{"mla":"Harish, Rohit K., et al. “Real-Time Monitoring of an Endogenous Fgf8a Gradient Attests to Its Role as a Morphogen during Zebrafish Gastrulation.” Development, vol. 150, no. 19, dev201559, The Company of Biologists, 2023, doi:10.1242/dev.201559.","short":"R.K. Harish, M. Gupta, D. Zöller, H. Hartmann, A. Gheisari, A. Machate, S. Hans, M. Brand, Development 150 (2023).","chicago":"Harish, Rohit K, Mansi Gupta, Daniela Zöller, Hella Hartmann, Ali Gheisari, Anja Machate, Stefan Hans, and Michael Brand. “Real-Time Monitoring of an Endogenous Fgf8a Gradient Attests to Its Role as a Morphogen during Zebrafish Gastrulation.” Development. The Company of Biologists, 2023. https://doi.org/10.1242/dev.201559.","ama":"Harish RK, Gupta M, Zöller D, et al. Real-time monitoring of an endogenous Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation. Development. 2023;150(19). doi:10.1242/dev.201559","ista":"Harish RK, Gupta M, Zöller D, Hartmann H, Gheisari A, Machate A, Hans S, Brand M. 2023. Real-time monitoring of an endogenous Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation. Development. 150(19), dev201559.","ieee":"R. K. Harish et al., “Real-time monitoring of an endogenous Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation,” Development, vol. 150, no. 19. The Company of Biologists, 2023.","apa":"Harish, R. K., Gupta, M., Zöller, D., Hartmann, H., Gheisari, A., Machate, A., … Brand, M. (2023). Real-time monitoring of an endogenous Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation. Development. The Company of Biologists. https://doi.org/10.1242/dev.201559"},"article_type":"original","date_published":"2023-10-01T00:00:00Z"},{"day":"01","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","scopus_import":"1","date_published":"2023-09-01T00:00:00Z","article_type":"original","publication":"Current Opinion in Systems Biology","citation":{"ista":"Minchington T, Rus S, Kicheva A. 2023. Control of tissue dimensions in the developing neural tube and somites. Current Opinion in Systems Biology. 35, 100459.","apa":"Minchington, T., Rus, S., & Kicheva, A. (2023). Control of tissue dimensions in the developing neural tube and somites. Current Opinion in Systems Biology. Elsevier. https://doi.org/10.1016/j.coisb.2023.100459","ieee":"T. Minchington, S. Rus, and A. Kicheva, “Control of tissue dimensions in the developing neural tube and somites,” Current Opinion in Systems Biology, vol. 35. Elsevier, 2023.","ama":"Minchington T, Rus S, Kicheva A. Control of tissue dimensions in the developing neural tube and somites. Current Opinion in Systems Biology. 2023;35. doi:10.1016/j.coisb.2023.100459","chicago":"Minchington, Thomas, Stefanie Rus, and Anna Kicheva. “Control of Tissue Dimensions in the Developing Neural Tube and Somites.” Current Opinion in Systems Biology. Elsevier, 2023. https://doi.org/10.1016/j.coisb.2023.100459.","mla":"Minchington, Thomas, et al. “Control of Tissue Dimensions in the Developing Neural Tube and Somites.” Current Opinion in Systems Biology, vol. 35, 100459, Elsevier, 2023, doi:10.1016/j.coisb.2023.100459.","short":"T. Minchington, S. Rus, A. Kicheva, Current Opinion in Systems Biology 35 (2023)."},"abstract":[{"lang":"eng","text":"Despite its fundamental importance for development, the question of how organs achieve their correct size and shape is poorly understood. This complex process requires coordination between the generation of cell mass and the morphogenetic mechanisms that sculpt tissues. These processes are regulated by morphogen signalling pathways and mechanical forces. Yet, in many systems, it is unclear how biochemical and mechanical signalling are quantitatively interpreted to determine the behaviours of individual cells and how they contribute to growth and morphogenesis at the tissue scale. In this review, we discuss the development of the vertebrate neural tube and somites as an example of the state of knowledge, as well as the challenges in understanding the mechanisms of tissue size control in vertebrate organogenesis. We highlight how the recent advances in stem cell differentiation and organoid approaches can be harnessed to provide new insights into this question."}],"type":"journal_article","oa_version":"Published Version","file":[{"checksum":"8a75c4e29fd9b62e3c50663c2265b173","success":1,"date_updated":"2024-01-29T11:06:45Z","date_created":"2024-01-29T11:06:45Z","relation":"main_file","file_id":"14896","content_type":"application/pdf","file_size":598842,"creator":"dernst","access_level":"open_access","file_name":"2023_CurrOpSystBioloy_Minchington.pdf"}],"title":"Control of tissue dimensions in the developing neural tube and somites","ddc":["570"],"status":"public","intvolume":" 35","_id":"13136","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"09","publication_identifier":{"eissn":["2452-3100"]},"language":[{"iso":"eng"}],"doi":"10.1016/j.coisb.2023.100459","quality_controlled":"1","project":[{"name":"Mechanisms of tissue size regulation in spinal cord development","_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","grant_number":"101044579"},{"name":"Morphogen control of growth and pattern in the spinal cord","_id":"059DF620-7A3F-11EA-A408-12923DDC885E","grant_number":"F07802"},{"name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube","grant_number":"SC19-011","_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A"}],"oa":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"file_date_updated":"2024-01-29T11:06:45Z","article_number":"100459","date_updated":"2024-01-29T11:07:47Z","date_created":"2023-06-18T22:00:46Z","volume":35,"author":[{"full_name":"Minchington, Thomas","last_name":"Minchington","first_name":"Thomas","id":"7d1648cb-19e9-11eb-8e7a-f8c037fb3e3f"},{"last_name":"Rus","first_name":"Stefanie","orcid":"0000-0001-8703-1093","id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","full_name":"Rus, Stefanie"},{"last_name":"Kicheva","first_name":"Anna","orcid":"0000-0003-4509-4998","id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","full_name":"Kicheva, Anna"}],"publication_status":"published","department":[{"_id":"AnKi"}],"publisher":"Elsevier","year":"2023","acknowledgement":"We thank J. Briscoe for comments on the manuscript. Work in the AK lab is supported by ISTA, the European Research Council under Horizon Europe: grant 101044579, and Austrian Science Fund (FWF): F78 (Stem Cell Modulation). SR is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011."}]